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The map label for this gene is eptA [H]

Identifier: 126174322

GI number: 126174322

Start: 2440726

End: 2442351

Strand: Direct

Name: eptA [H]

Synonym: Sbal_2103

Alternate gene names: 126174322

Gene position: 2440726-2442351 (Clockwise)

Preceding gene: 126174321

Following gene: 126174323

Centisome position: 47.6

GC content: 43.6

Gene sequence:

>1626_bases
GTGCTAGCAAGATTTAAGACTCTATCGGTGAATCGATTCACTTTTATCACAGCGTTATTTTACGTTTGTATCTTCAATAT
CCCACTATTCGGCATCATTAGAAAAGGCATCGAAAAACAACCGGATGTCGATCCTATTTTCATCGCCACTATGCCGTTGT
TTTTAGCCTTTGCCCTCAGTTTTGTGTTTTCCTTATTTACGGTGAAGTATCTCGTTAAGCCATTTTTCATCATACTCACC
TTGTTGTCATCGAGTGTGTTCTTTGCCGCCTATCAATACAATGTCGTGTTTGATTACGGCATGATAGAAAACACCTTTCA
AACCAATCATGCCGAAGCGCTGATGTATGTGAATTATGCTTCGATAATGAATATGTTACTGACAGGGATTTTACCTGCGT
ACCTTATCTATAAGGCCGATATTCAGTACCAGCCTTTCTTCAAAGAGTTACTGCATAAAGTGCTGTTTATGCTGGCGATG
TTGGCGGGGCTCGGCATTATTGCTTTCTTTTATTTTCAAGATTACGCCGCCTTTGGCCGCAACAACGACGAGCTAAAACG
TTATATCGTACCGACGTATTTTATTGGCGCTGCGTCTAAGTACATTAATGTGCACTACTTACAAACGCCGATTGAATATC
AGCCACTGGGTCTCGATGCCAAAAATGTCAGTGCTAACCCTAATGGTAAACCTAACTTATTAGTGCTTGTGGTCGGTGAA
ACCGCGCGTTCGATGAGTTATCAGTATTATGGTTATGACAAGCCAACCAACGCCCATACCCAAGATCAAGGCTTGATTGT
CTTTAAAGATACCAGTTCATGTGGCACGGCAACGGCAGTCTCCCTGCCTTGCATGTTCTCGCGCATGGGACGGGAAGATT
ACGATTCTCGCCGCGCCTATGCTCAAGATACTGCGGTAGATGTGTTGAACCATGGCGGTATTAAAGTGCAATGGTTTGAT
AACGACTCTGGCTGTAAAGGGGTGTGCGATCAAGTTGAAAACATTTCGATTGATTTGAACAGCGATCCTGAGTTGTGTAC
TGGCCAATATTGTTTCGACCAAGTGTTACTCAACAAATTAGATGAAACCTTGGCCACTGCAGAACGCAAAGATACTGTGA
TTGTGTTGCATGTGATTGGTAGCCATGGACCGACGTATTTCTTACGTTATCCACCGGAGCACCGTAAGTTCACACCGGAT
TGTCAGCGCAGCGATATCCAAAACTGTAGCCCAGATGAGCTGATGAACACCTACGACAACACCATTTTGTATACTGACTA
CATCATCAGCGAAGTAGTGAAAAAGTTACAAAATCAGCAAGATAAATTTGATCCAGCCATGTTGTATCTCAGCGACCATG
GCGAATCTTTAGGCGAGAAGGGCATGTATTTACATGGCGCACCTTACAGCATTGCACCAATCGAACAGACGAGTATTCCT
ATGCTTGCTTGGGTATCTGAGGATTTCAGTAAAGATAACCACTTAGACATGGCCTGTTTAGCTGAGCACGCGGCGAAGGG
CGGTTTCTCCCATGACAATTTATTCGACAGTCTTTTAGGCCTAATGAATGTCAAGACCGAGGTATATCAGCCTAAGTTAG
ATATCTTTGCCAGTTGCCGAGGTTAA

Upstream 100 bases:

>100_bases
GCTTGCTACTATGGCAAAAAATTTGACTGTTACAGCCTAAGATTACCTTAAGAATATTAAGTTAGTCTCGACAAACAACT
AATTTGAGTGAGAGCTATCA

Downstream 100 bases:

>100_bases
TCAACCCATTAACTCATTAATATTGAGTCGATAACATTATCGTGCGACTCAATGTTATCTTGTGACTAAGTATGATCTAC
TGACCAAAAGTTATTGAGTG

Product: hypothetical protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MLARFKTLSVNRFTFITALFYVCIFNIPLFGIIRKGIEKQPDVDPIFIATMPLFLAFALSFVFSLFTVKYLVKPFFIILT
LLSSSVFFAAYQYNVVFDYGMIENTFQTNHAEALMYVNYASIMNMLLTGILPAYLIYKADIQYQPFFKELLHKVLFMLAM
LAGLGIIAFFYFQDYAAFGRNNDELKRYIVPTYFIGAASKYINVHYLQTPIEYQPLGLDAKNVSANPNGKPNLLVLVVGE
TARSMSYQYYGYDKPTNAHTQDQGLIVFKDTSSCGTATAVSLPCMFSRMGREDYDSRRAYAQDTAVDVLNHGGIKVQWFD
NDSGCKGVCDQVENISIDLNSDPELCTGQYCFDQVLLNKLDETLATAERKDTVIVLHVIGSHGPTYFLRYPPEHRKFTPD
CQRSDIQNCSPDELMNTYDNTILYTDYIISEVVKKLQNQQDKFDPAMLYLSDHGESLGEKGMYLHGAPYSIAPIEQTSIP
MLAWVSEDFSKDNHLDMACLAEHAAKGGFSHDNLFDSLLGLMNVKTEVYQPKLDIFASCRG

Sequences:

>Translated_541_residues
MLARFKTLSVNRFTFITALFYVCIFNIPLFGIIRKGIEKQPDVDPIFIATMPLFLAFALSFVFSLFTVKYLVKPFFIILT
LLSSSVFFAAYQYNVVFDYGMIENTFQTNHAEALMYVNYASIMNMLLTGILPAYLIYKADIQYQPFFKELLHKVLFMLAM
LAGLGIIAFFYFQDYAAFGRNNDELKRYIVPTYFIGAASKYINVHYLQTPIEYQPLGLDAKNVSANPNGKPNLLVLVVGE
TARSMSYQYYGYDKPTNAHTQDQGLIVFKDTSSCGTATAVSLPCMFSRMGREDYDSRRAYAQDTAVDVLNHGGIKVQWFD
NDSGCKGVCDQVENISIDLNSDPELCTGQYCFDQVLLNKLDETLATAERKDTVIVLHVIGSHGPTYFLRYPPEHRKFTPD
CQRSDIQNCSPDELMNTYDNTILYTDYIISEVVKKLQNQQDKFDPAMLYLSDHGESLGEKGMYLHGAPYSIAPIEQTSIP
MLAWVSEDFSKDNHLDMACLAEHAAKGGFSHDNLFDSLLGLMNVKTEVYQPKLDIFASCRG
>Mature_541_residues
MLARFKTLSVNRFTFITALFYVCIFNIPLFGIIRKGIEKQPDVDPIFIATMPLFLAFALSFVFSLFTVKYLVKPFFIILT
LLSSSVFFAAYQYNVVFDYGMIENTFQTNHAEALMYVNYASIMNMLLTGILPAYLIYKADIQYQPFFKELLHKVLFMLAM
LAGLGIIAFFYFQDYAAFGRNNDELKRYIVPTYFIGAASKYINVHYLQTPIEYQPLGLDAKNVSANPNGKPNLLVLVVGE
TARSMSYQYYGYDKPTNAHTQDQGLIVFKDTSSCGTATAVSLPCMFSRMGREDYDSRRAYAQDTAVDVLNHGGIKVQWFD
NDSGCKGVCDQVENISIDLNSDPELCTGQYCFDQVLLNKLDETLATAERKDTVIVLHVIGSHGPTYFLRYPPEHRKFTPD
CQRSDIQNCSPDELMNTYDNTILYTDYIISEVVKKLQNQQDKFDPAMLYLSDHGESLGEKGMYLHGAPYSIAPIEQTSIP
MLAWVSEDFSKDNHLDMACLAEHAAKGGFSHDNLFDSLLGLMNVKTEVYQPKLDIFASCRG

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=530, Percent_Identity=37.7358490566038, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI87082286, Length=472, Percent_Identity=24.7881355932203, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082223, Length=451, Percent_Identity=23.9467849223947, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790392, Length=283, Percent_Identity=24.0282685512367, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61254; Mature: 61254

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLARFKTLSVNRFTFITALFYVCIFNIPLFGIIRKGIEKQPDVDPIFIATMPLFLAFALS
CCCCEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHH
FVFSLFTVKYLVKPFFIILTLLSSSVFFAAYQYNVVFDYGMIENTFQTNHAEALMYVNYA
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEHH
SIMNMLLTGILPAYLIYKADIQYQPFFKELLHKVLFMLAMLAGLGIIAFFYFQDYAAFGR
HHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNDELKRYIVPTYFIGAASKYINVHYLQTPIEYQPLGLDAKNVSANPNGKPNLLVLVVGE
CHHHHHHHCCCHHEECCHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
TARSMSYQYYGYDKPTNAHTQDQGLIVFKDTSSCGTATAVSLPCMFSRMGREDYDSRRAY
CCHHCCEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
AQDTAVDVLNHGGIKVQWFDNDSGCKGVCDQVENISIDLNSDPELCTGQYCFDQVLLNKL
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHH
DETLATAERKDTVIVLHVIGSHGPTYFLRYPPEHRKFTPDCQRSDIQNCSPDELMNTYDN
HHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
TILYTDYIISEVVKKLQNQQDKFDPAMLYLSDHGESLGEKGMYLHGAPYSIAPIEQTSIP
EEEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCEECCCCCCCCC
MLAWVSEDFSKDNHLDMACLAEHAAKGGFSHDNLFDSLLGLMNVKTEVYQPKLDIFASCR
EEEEHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
G
C
>Mature Secondary Structure
MLARFKTLSVNRFTFITALFYVCIFNIPLFGIIRKGIEKQPDVDPIFIATMPLFLAFALS
CCCCEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHH
FVFSLFTVKYLVKPFFIILTLLSSSVFFAAYQYNVVFDYGMIENTFQTNHAEALMYVNYA
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEHH
SIMNMLLTGILPAYLIYKADIQYQPFFKELLHKVLFMLAMLAGLGIIAFFYFQDYAAFGR
HHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNDELKRYIVPTYFIGAASKYINVHYLQTPIEYQPLGLDAKNVSANPNGKPNLLVLVVGE
CHHHHHHHCCCHHEECCHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
TARSMSYQYYGYDKPTNAHTQDQGLIVFKDTSSCGTATAVSLPCMFSRMGREDYDSRRAY
CCHHCCEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
AQDTAVDVLNHGGIKVQWFDNDSGCKGVCDQVENISIDLNSDPELCTGQYCFDQVLLNKL
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHH
DETLATAERKDTVIVLHVIGSHGPTYFLRYPPEHRKFTPDCQRSDIQNCSPDELMNTYDN
HHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
TILYTDYIISEVVKKLQNQQDKFDPAMLYLSDHGESLGEKGMYLHGAPYSIAPIEQTSIP
EEEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCEECCCCCCCCC
MLAWVSEDFSKDNHLDMACLAEHAAKGGFSHDNLFDSLLGLMNVKTEVYQPKLDIFASCR
EEEEHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]