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The map label for this gene is pleD [H]

Identifier: 126174323

GI number: 126174323

Start: 2442623

End: 2443651

Strand: Direct

Name: pleD [H]

Synonym: Sbal_2104

Alternate gene names: 126174323

Gene position: 2442623-2443651 (Clockwise)

Preceding gene: 126174322

Following gene: 126174325

Centisome position: 47.64

GC content: 45.19

Gene sequence:

>1029_bases
ATGTCTTCTACTGCAGAAAATAAAGAGCTTGATTCTGCCGCGCAAATTTTGCGTTTGGCCGTACCGCAAATGTCTGCCCT
CGATATCCCCGTTACACCTGAAAACTACACTGTTTGGTATGAATATTTTGCAGGGACAAATCTCGATTTAAACCGTGCGA
TTGATGGCTTTCTGGCTAATTCGGTCTCTTTTACTAAGGAGGTCAATACCAGCCTGTATAAGAACTTTATTCAAGAACAG
TCACCCGAAGTGATTGAAAATGTTCAGATTGAAACGCAAATCCTGATCAATAGTTTGTTAAGTAAAATCAGCCAGTTGAG
TCAAGGCACCGCGGCGTTTTCAGGTTCATTAGCCGAGTTTGGTTTACGCCTACAGACAAATCCCGACATTAATACCTTAA
ACGAGCTGGTTGACGGTGTAGTCGATGAAATGCAGATGCTACTCATCAACAACCGTGAGATGGAGCAAAGCCTGTGCACC
ATGGGCCAAGAAGTGCAAAATCTTAAATCGGAAATGGAAAACCTGAGCCTAGCAGCGATGACTGACCAATTAACCTCGCT
GCACAATCGCCGTGCCTACGAGTTAAGTATTCAAGATCAAATCTACCTCTCCCAAGATCACCAAACCCGTTGCAGTCTTT
TACTGATCGATATTGATAATTTTAAAGCCTTTAACGACACCTATGGTCATTTGACCGGCGATAAAGTGTTGGCCTATGTC
GCATTAGCCTTGAAACAATGTGTGCGAGGGGATGACTTTGTGGCGCGCTATGGCGGCGAAGAATTTGTGGTGGTGTTACC
TAACACTCATTTTCACGATGCATTACAAGTGGCTGAAAATCTTCGGGAACGTATCTCTGAGCGCCGCTTAACCATAGGGA
AAGACAAAAAGCTTTCCCTTGGCGCCATTACTGTTTCGATTGGTCTGGCTTCATTGCAGCAAGGTGACGATGCAGAAACC
CTATTCGGTCGCGCCGACAAAGCGCTATATCAAGCTAAGGCCGAAGGCCGTAACTGCGTGAGAGGATAA

Upstream 100 bases:

>100_bases
TGCGCCCAATCATAATAATTGATTTCTCCCATATCCAAATCGAAGCACTTGAGTTAACTTAATGCATACAAAAACATAAC
AAGTCTATGGTTCGCGATTT

Downstream 100 bases:

>100_bases
AGAGATAGGGGATTTGCGGCTGAGTTTTTGCTAGTTCAGTTACCATAAACAGGTTGATTTTGCTGCGTTTATTCAGCCAA
TGGTGCACGGCCTAGGCTCA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSSTAENKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAGTNLDLNRAIDGFLANSVSFTKEVNTSLYKNFIQEQ
SPEVIENVQIETQILINSLLSKISQLSQGTAAFSGSLAEFGLRLQTNPDINTLNELVDGVVDEMQMLLINNREMEQSLCT
MGQEVQNLKSEMENLSLAAMTDQLTSLHNRRAYELSIQDQIYLSQDHQTRCSLLLIDIDNFKAFNDTYGHLTGDKVLAYV
ALALKQCVRGDDFVARYGGEEFVVVLPNTHFHDALQVAENLRERISERRLTIGKDKKLSLGAITVSIGLASLQQGDDAET
LFGRADKALYQAKAEGRNCVRG

Sequences:

>Translated_342_residues
MSSTAENKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAGTNLDLNRAIDGFLANSVSFTKEVNTSLYKNFIQEQ
SPEVIENVQIETQILINSLLSKISQLSQGTAAFSGSLAEFGLRLQTNPDINTLNELVDGVVDEMQMLLINNREMEQSLCT
MGQEVQNLKSEMENLSLAAMTDQLTSLHNRRAYELSIQDQIYLSQDHQTRCSLLLIDIDNFKAFNDTYGHLTGDKVLAYV
ALALKQCVRGDDFVARYGGEEFVVVLPNTHFHDALQVAENLRERISERRLTIGKDKKLSLGAITVSIGLASLQQGDDAET
LFGRADKALYQAKAEGRNCVRG
>Mature_341_residues
SSTAENKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAGTNLDLNRAIDGFLANSVSFTKEVNTSLYKNFIQEQS
PEVIENVQIETQILINSLLSKISQLSQGTAAFSGSLAEFGLRLQTNPDINTLNELVDGVVDEMQMLLINNREMEQSLCTM
GQEVQNLKSEMENLSLAAMTDQLTSLHNRRAYELSIQDQIYLSQDHQTRCSLLLIDIDNFKAFNDTYGHLTGDKVLAYVA
LALKQCVRGDDFVARYGGEEFVVVLPNTHFHDALQVAENLRERISERRLTIGKDKKLSLGAITVSIGLASLQQGDDAETL
FGRADKALYQAKAEGRNCVRG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=220, Percent_Identity=37.2727272727273, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI145693134, Length=271, Percent_Identity=33.9483394833948, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87082007, Length=197, Percent_Identity=37.0558375634518, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=31.638418079096, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=36.6666666666667, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=36.7469879518072, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787541, Length=193, Percent_Identity=36.7875647668394, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=33.5294117647059, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=191, Percent_Identity=31.413612565445, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=30.1675977653631, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=29.1925465838509, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=168, Percent_Identity=32.1428571428571, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081977, Length=170, Percent_Identity=31.1764705882353, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787056, Length=196, Percent_Identity=31.6326530612245, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38084; Mature: 37953

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTAENKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAGTNLDLNRAIDGFLAN
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHC
SVSFTKEVNTSLYKNFIQEQSPEVIENVQIETQILINSLLSKISQLSQGTAAFSGSLAEF
CHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHC
GLRLQTNPDINTLNELVDGVVDEMQMLLINNREMEQSLCTMGQEVQNLKSEMENLSLAAM
CEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDQLTSLHNRRAYELSIQDQIYLSQDHQTRCSLLLIDIDNFKAFNDTYGHLTGDKVLAYV
HHHHHHHHCCCEEEEEECCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
ALALKQCVRGDDFVARYGGEEFVVVLPNTHFHDALQVAENLRERISERRLTIGKDKKLSL
HHHHHHHHCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
GAITVSIGLASLQQGDDAETLFGRADKALYQAKAEGRNCVRG
EEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSTAENKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAGTNLDLNRAIDGFLAN
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHC
SVSFTKEVNTSLYKNFIQEQSPEVIENVQIETQILINSLLSKISQLSQGTAAFSGSLAEF
CHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHC
GLRLQTNPDINTLNELVDGVVDEMQMLLINNREMEQSLCTMGQEVQNLKSEMENLSLAAM
CEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDQLTSLHNRRAYELSIQDQIYLSQDHQTRCSLLLIDIDNFKAFNDTYGHLTGDKVLAYV
HHHHHHHHCCCEEEEEECCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
ALALKQCVRGDDFVARYGGEEFVVVLPNTHFHDALQVAENLRERISERRLTIGKDKKLSL
HHHHHHHHCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
GAITVSIGLASLQQGDDAETLFGRADKALYQAKAEGRNCVRG
EEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA