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The map label for this gene is ruvC

Identifier: 126174262

GI number: 126174262

Start: 2365343

End: 2365864

Strand: Direct

Name: ruvC

Synonym: Sbal_2040

Alternate gene names: 126174262

Gene position: 2365343-2365864 (Clockwise)

Preceding gene: 126174261

Following gene: 126174263

Centisome position: 46.13

GC content: 50.96

Gene sequence:

>522_bases
ATGGCAATTATTTTAGGTGTTGACCCAGGTTCACGGATCACAGGCTACGGCGTGATCCAATGTCAGGGGCGGCATCAGAT
TTACCTCGGCAGTGGCTGTATTCGCACTTCGTCTGAAGAGCTTCCTGGCAGGCTTAAGCAGATATTTGATGGGATCACAG
AGATTATTCGTCAGTATCAACCGGACGAGTTTGCGATTGAGCGAGTATTTATGGCAAAAAATGCCGATTCTGCGCTCAAG
CTTGGCCAAGCACGTGGCGCGGCGATTGTCGCCGCCACGGTCGCCAATTTACCTGTGGCTGAATACAGTGCGACTCAAAT
TAAAAGCGCAGTCGTTGGCACAGGGCGAGCACAGAAAGCTCAAGTACAACACATGATCCAACAACTACTTAAATTACCCG
CAGCCCCACAAGCTGACGCCGCAGATGCACTCGGTGTTGCTGTGTGTCATTATCACACCAGCCAGAGTTTAATTGCATTG
AGTGGCCGGGCAACGGCAAGAACATACGGACGGTACAGATGA

Upstream 100 bases:

>100_bases
GGTCGTCAATCTAGTCCAATTTCAGCTCGCACACGCTAACCTAGAACACTCGCTAATCCAGGAACTGAGACGAACATTCA
GATCCCATATAAGGCATTAA

Downstream 100 bases:

>100_bases
TAGGTCGTTTACGGGGCGTGTTAGTTGAGAAACAAGCGCCAGAAATTTTGATCGATGTTAATGGTGTGGGTTACGAGTTG
CAGATGCCGCTCACCAGTTT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MAIILGVDPGSRITGYGVIQCQGRHQIYLGSGCIRTSSEELPGRLKQIFDGITEIIRQYQPDEFAIERVFMAKNADSALK
LGQARGAAIVAATVANLPVAEYSATQIKSAVVGTGRAQKAQVQHMIQQLLKLPAAPQADAADALGVAVCHYHTSQSLIAL
SGRATARTYGRYR

Sequences:

>Translated_173_residues
MAIILGVDPGSRITGYGVIQCQGRHQIYLGSGCIRTSSEELPGRLKQIFDGITEIIRQYQPDEFAIERVFMAKNADSALK
LGQARGAAIVAATVANLPVAEYSATQIKSAVVGTGRAQKAQVQHMIQQLLKLPAAPQADAADALGVAVCHYHTSQSLIAL
SGRATARTYGRYR
>Mature_172_residues
AIILGVDPGSRITGYGVIQCQGRHQIYLGSGCIRTSSEELPGRLKQIFDGITEIIRQYQPDEFAIERVFMAKNADSALKL
GQARGAAIVAATVANLPVAEYSATQIKSAVVGTGRAQKAQVQHMIQQLLKLPAAPQADAADALGVAVCHYHTSQSLIALS
GRATARTYGRYR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=173, Percent_Identity=65.3179190751445, Blast_Score=208, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_SHEB2 (B8EA73)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357969.1
- ProteinModelPortal:   B8EA73
- SMR:   B8EA73
- GeneID:   7086876
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_2042
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- ProtClustDB:   PRK00039
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18496; Mature: 18364

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIILGVDPGSRITGYGVIQCQGRHQIYLGSGCIRTSSEELPGRLKQIFDGITEIIRQYQ
CEEEEECCCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PDEFAIERVFMAKNADSALKLGQARGAAIVAATVANLPVAEYSATQIKSAVVGTGRAQKA
CCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCCHHHH
QVQHMIQQLLKLPAAPQADAADALGVAVCHYHTSQSLIALSGRATARTYGRYR
HHHHHHHHHHHCCCCCCCCCHHHHHHHEEEEECCCCEEEECCCCHHHHCCCCC
>Mature Secondary Structure 
AIILGVDPGSRITGYGVIQCQGRHQIYLGSGCIRTSSEELPGRLKQIFDGITEIIRQYQ
EEEEECCCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PDEFAIERVFMAKNADSALKLGQARGAAIVAATVANLPVAEYSATQIKSAVVGTGRAQKA
CCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHCCCCHHHH
QVQHMIQQLLKLPAAPQADAADALGVAVCHYHTSQSLIALSGRATARTYGRYR
HHHHHHHHHHHCCCCCCCCCHHHHHHHEEEEECCCCEEEECCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA