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The map label for this gene is ruvA

Identifier: 126174263

GI number: 126174263

Start: 2365861

End: 2366478

Strand: Direct

Name: ruvA

Synonym: Sbal_2041

Alternate gene names: 126174263

Gene position: 2365861-2366478 (Clockwise)

Preceding gene: 126174262

Following gene: 126174264

Centisome position: 46.14

GC content: 46.93

Gene sequence:

>618_bases
ATGATAGGTCGTTTACGGGGCGTGTTAGTTGAGAAACAAGCGCCAGAAATTTTGATCGATGTTAATGGTGTGGGTTACGA
GTTGCAGATGCCGCTCACCAGTTTTTATGAGCTGCCTGAAGTTAACCATGAAACTATGGTCTACACCCATTTTGTGGTGC
GTGAAGATGCACAGTTACTTTATGGTTTTATCACTAAGCAGGAAAGGGCGTTATTTCGCTTATTGATCAAGACCAACGGT
GTCGGCCCCAAGCTTGCTCTGACCATTTTATCTGGCATGACAGCCGGAGAATTTGTGGGCTGTGTTGAGCGTGATGACAT
AGTGACCTTAGTGAAATTACCCGGTGTGGGTAAGAAGACCGCCGAGCGTTTACTGGTAGAAATGCGCGATAAGCTCAAGA
GCTTGATGGAAGCCTCAGCAGGTTCAGAGCGTGAGTTCGTACTGCAATCTAATTACTCACCCGCGCCGACAGTTAATAGC
GCCGAAGAAGATGCCATTTCAGCTTTGATTTCGCTGGGTTATAAACCGCCGCAGGCGAGTAAATCAGTTTCTGCCGCTTA
TAAAGAGGGCATGGATTCAGAGACTTTGATCAAAGCTGCCCTTAAGTCGATGCTTTGA

Upstream 100 bases:

>100_bases
CCGCAGATGCACTCGGTGTTGCTGTGTGTCATTATCACACCAGCCAGAGTTTAATTGCATTGAGTGGCCGGGCAACGGCA
AGAACATACGGACGGTACAG

Downstream 100 bases:

>100_bases
TTGAACAATGATGATTATTAAGATGAAAGCTGCGAGAAGGAAGAACAATGATTGAGGCAGACAGACTCATCCAGCCGCAG
ATCCAAGCTCAAGATGAATC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MIGRLRGVLVEKQAPEILIDVNGVGYELQMPLTSFYELPEVNHETMVYTHFVVREDAQLLYGFITKQERALFRLLIKTNG
VGPKLALTILSGMTAGEFVGCVERDDIVTLVKLPGVGKKTAERLLVEMRDKLKSLMEASAGSEREFVLQSNYSPAPTVNS
AEEDAISALISLGYKPPQASKSVSAAYKEGMDSETLIKAALKSML

Sequences:

>Translated_205_residues
MIGRLRGVLVEKQAPEILIDVNGVGYELQMPLTSFYELPEVNHETMVYTHFVVREDAQLLYGFITKQERALFRLLIKTNG
VGPKLALTILSGMTAGEFVGCVERDDIVTLVKLPGVGKKTAERLLVEMRDKLKSLMEASAGSEREFVLQSNYSPAPTVNS
AEEDAISALISLGYKPPQASKSVSAAYKEGMDSETLIKAALKSML
>Mature_205_residues
MIGRLRGVLVEKQAPEILIDVNGVGYELQMPLTSFYELPEVNHETMVYTHFVVREDAQLLYGFITKQERALFRLLIKTNG
VGPKLALTILSGMTAGEFVGCVERDDIVTLVKLPGVGKKTAERLLVEMRDKLKSLMEASAGSEREFVLQSNYSPAPTVNS
AEEDAISALISLGYKPPQASKSVSAAYKEGMDSETLIKAALKSML

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=205, Percent_Identity=60, Blast_Score=248, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_SHEB2 (B8EA74)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357970.1
- ProteinModelPortal:   B8EA74
- SMR:   B8EA74
- GeneID:   7086877
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_2043
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22483; Mature: 22483

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRLRGVLVEKQAPEILIDVNGVGYELQMPLTSFYELPEVNHETMVYTHFVVREDAQLL
CCCCHHHEEECCCCCEEEEEECCCCEEEECCHHHHHHCCCCCCCEEEEEEHHHHHHHHHH
YGFITKQERALFRLLIKTNGVGPKLALTILSGMTAGEFVGCVERDDIVTLVKLPGVGKKT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCCHHH
AERLLVEMRDKLKSLMEASAGSEREFVLQSNYSPAPTVNSAEEDAISALISLGYKPPQAS
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCH
KSVSAAYKEGMDSETLIKAALKSML
HHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIGRLRGVLVEKQAPEILIDVNGVGYELQMPLTSFYELPEVNHETMVYTHFVVREDAQLL
CCCCHHHEEECCCCCEEEEEECCCCEEEECCHHHHHHCCCCCCCEEEEEEHHHHHHHHHH
YGFITKQERALFRLLIKTNGVGPKLALTILSGMTAGEFVGCVERDDIVTLVKLPGVGKKT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCCHHH
AERLLVEMRDKLKSLMEASAGSEREFVLQSNYSPAPTVNSAEEDAISALISLGYKPPQAS
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCH
KSVSAAYKEGMDSETLIKAALKSML
HHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA