Click here to switch to the map view.

The map label for this gene is thiF [H]

Identifier: 126174246

GI number: 126174246

Start: 2344922

End: 2345851

Strand: Direct

Name: thiF [H]

Synonym: Sbal_2024

Alternate gene names: 126174246

Gene position: 2344922-2345851 (Clockwise)

Preceding gene: 126174245

Following gene: 126174247

Centisome position: 45.73

GC content: 48.71

Gene sequence:

>930_bases
ATGAGTTGGATGCAAAGCATGAGTAAAAACCCACCGCTTGCTCACGCAGAGGTTCTTTGCGGTCATATACACAGCGACAA
TGAACACTGCGGCCATACACTCAGTGATGCCGATTTCATCCGTTATTCTCGGCAAGTCTTATTGCCAGAAGTGGGTGAGG
CCGGGCAATTGCAACTAGCCGAAGCCGGTGTCGCTATTATCGGTCTTGGCGGTTTAGGGCAATTGGCGGCGCAGTATCTG
GCTTGTGCAGGTATTGGTCGCTTGACCTTAATCGATAGGGATAAGGTTGAAGTATCGAATTTGCCAAGACAATTGTTATT
CAACGATGCCGATATTGGACTCAATAAGGCGCGAGTTGCCAAGCAAAAGCTCAAAGGCTTAGCGCCGCAGTGCACTGTTA
CTGCCTATGAAACAGCATTTAATCCTGCGACATCGGCTCATCATTTGGCGGATATTTTACAAATAAAACAACAAGGTAAA
AGAGTACTTGTGCTTGACTGCACCGATAACTTTGCGACTCGCCAAGCCATTAATCGCCGCTGTATCGAAGCGGCTTTGCC
TTTAGTTAGCGCGTCAATCGCAGCATTTAGCGGCCAATTGTTCGCGGTCGACCAAATGCGGTTCCCCTCTGGTGGTTGTT
ATCACTGTATTTTCTCCGCACAGACGAGAGTGTCGCAGAGCTGCAGTACCCAAGGCGTACTCGGCCCCAGCGTGGGCGTG
ATGGCGTCGATGCAATCTTTGGTGGCCATGCAACTCTTGCTGAATATGGATAGATGTGATGAATCGAAAAATGTCTTGTT
GGGACGTTTTTGGCGCTTCGACGCTAAATCACTTTCATGGACAGCGGCGATATTAACGCGGGATCCCCATTGTGACGTAT
GTGGTCCAAAAGAGGTTCATTCATCATCCGATAAGCCCAGAAAACTATAA

Upstream 100 bases:

>100_bases
GTGCGCGCAATAACGAAAGCTAAGGATCCGTTAGCCGCCTTTGCAGAGTTGAGCCAAGCTTGGGAGCAATGTAGCTTGTC
TGAAGAACTGGCTGTAAAGC

Downstream 100 bases:

>100_bases
AGTATGGAGGTTTGATATGATTTTAATTCACATAAATAATGTCCCGCAGCCATTAACGGAACCGACTTCACTCGCGGCGG
TGATCCTCGCGCAAGATATA

Product: UBA/THIF-type NAD/FAD binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSWMQSMSKNPPLAHAEVLCGHIHSDNEHCGHTLSDADFIRYSRQVLLPEVGEAGQLQLAEAGVAIIGLGGLGQLAAQYL
ACAGIGRLTLIDRDKVEVSNLPRQLLFNDADIGLNKARVAKQKLKGLAPQCTVTAYETAFNPATSAHHLADILQIKQQGK
RVLVLDCTDNFATRQAINRRCIEAALPLVSASIAAFSGQLFAVDQMRFPSGGCYHCIFSAQTRVSQSCSTQGVLGPSVGV
MASMQSLVAMQLLLNMDRCDESKNVLLGRFWRFDAKSLSWTAAILTRDPHCDVCGPKEVHSSSDKPRKL

Sequences:

>Translated_309_residues
MSWMQSMSKNPPLAHAEVLCGHIHSDNEHCGHTLSDADFIRYSRQVLLPEVGEAGQLQLAEAGVAIIGLGGLGQLAAQYL
ACAGIGRLTLIDRDKVEVSNLPRQLLFNDADIGLNKARVAKQKLKGLAPQCTVTAYETAFNPATSAHHLADILQIKQQGK
RVLVLDCTDNFATRQAINRRCIEAALPLVSASIAAFSGQLFAVDQMRFPSGGCYHCIFSAQTRVSQSCSTQGVLGPSVGV
MASMQSLVAMQLLLNMDRCDESKNVLLGRFWRFDAKSLSWTAAILTRDPHCDVCGPKEVHSSSDKPRKL
>Mature_308_residues
SWMQSMSKNPPLAHAEVLCGHIHSDNEHCGHTLSDADFIRYSRQVLLPEVGEAGQLQLAEAGVAIIGLGGLGQLAAQYLA
CAGIGRLTLIDRDKVEVSNLPRQLLFNDADIGLNKARVAKQKLKGLAPQCTVTAYETAFNPATSAHHLADILQIKQQGKR
VLVLDCTDNFATRQAINRRCIEAALPLVSASIAAFSGQLFAVDQMRFPSGGCYHCIFSAQTRVSQSCSTQGVLGPSVGVM
ASMQSLVAMQLLLNMDRCDESKNVLLGRFWRFDAKSLSWTAAILTRDPHCDVCGPKEVHSSSDKPRKL

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=185, Percent_Identity=44.3243243243243, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI4885649, Length=181, Percent_Identity=30.3867403314917, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI13376212, Length=265, Percent_Identity=27.9245283018868, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI38327032, Length=243, Percent_Identity=27.5720164609054, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI87082356, Length=263, Percent_Identity=41.06463878327, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1787048, Length=263, Percent_Identity=35.7414448669202, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1789177, Length=139, Percent_Identity=31.6546762589928, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540406, Length=268, Percent_Identity=34.7014925373134, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI193203301, Length=165, Percent_Identity=29.0909090909091, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=267, Percent_Identity=31.4606741573034, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24582879, Length=281, Percent_Identity=36.6548042704626, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24660640, Length=178, Percent_Identity=29.7752808988764, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24641311, Length=245, Percent_Identity=29.3877551020408, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 33501; Mature: 33370

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWMQSMSKNPPLAHAEVLCGHIHSDNEHCGHTLSDADFIRYSRQVLLPEVGEAGQLQLA
CCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
EAGVAIIGLGGLGQLAAQYLACAGIGRLTLIDRDKVEVSNLPRQLLFNDADIGLNKARVA
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCHHHHCCCCCCCCHHHHHH
KQKLKGLAPQCTVTAYETAFNPATSAHHLADILQIKQQGKRVLVLDCTDNFATRQAINRR
HHHHHCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
CIEAALPLVSASIAAFSGQLFAVDQMRFPSGGCYHCIFSAQTRVSQSCSTQGVLGPSVGV
HHHHHHHHHHHHHHHHCCCEEEEECEECCCCCEEEEEECHHHHHHHHCCCCCCCCCCHHH
MASMQSLVAMQLLLNMDRCDESKNVLLGRFWRFDAKSLSWTAAILTRDPHCDVCGPKEVH
HHHHHHHHHHHHHHHHHHCCCCCCEEEEHEEECCCCCCEEEEEEEECCCCCCCCCCHHHH
SSSDKPRKL
CCCCCCCCC
>Mature Secondary Structure 
SWMQSMSKNPPLAHAEVLCGHIHSDNEHCGHTLSDADFIRYSRQVLLPEVGEAGQLQLA
CHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
EAGVAIIGLGGLGQLAAQYLACAGIGRLTLIDRDKVEVSNLPRQLLFNDADIGLNKARVA
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCHHHHCCCCCCCCHHHHHH
KQKLKGLAPQCTVTAYETAFNPATSAHHLADILQIKQQGKRVLVLDCTDNFATRQAINRR
HHHHHCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
CIEAALPLVSASIAAFSGQLFAVDQMRFPSGGCYHCIFSAQTRVSQSCSTQGVLGPSVGV
HHHHHHHHHHHHHHHHCCCEEEEECEECCCCCEEEEEECHHHHHHHHCCCCCCCCCCHHH
MASMQSLVAMQLLLNMDRCDESKNVLLGRFWRFDAKSLSWTAAILTRDPHCDVCGPKEVH
HHHHHHHHHHHHHHHHHHCCCCCCEEEEHEEECCCCCCEEEEEEEECCCCCCCCCCHHHH
SSSDKPRKL
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]