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The map label for this gene is thiE [H]

Identifier: 126174245

GI number: 126174245

Start: 2342995

End: 2344947

Strand: Direct

Name: thiE [H]

Synonym: Sbal_2023

Alternate gene names: 126174245

Gene position: 2342995-2344947 (Clockwise)

Preceding gene: 126174244

Following gene: 126174246

Centisome position: 45.7

GC content: 48.44

Gene sequence:

>1953_bases
ATGTTGGGTATTCATGGGGATAATGCGCCCATGAATACCGAGCATCCCGCGTTTGTTTGGACCATAGCTGGTTCAGATAG
TGGCGGTGGTGCCGGTATTCAGGCGGACTTAGCGACAATTCAAGATTTGGGCTGTCACGGTTGCAGTGTGGTCACCACAG
TGACGGCGCAAAGTTCTGTGGCGGTCACTTTAGTCGAGCCCGTATCAGCGGCGATGTTAATGGCTCAGCTGACGACTTTA
CTTTCCGACTTACCACCCAAAGCAATTAAAATCGGCTTACTGGCAGATCAAACTCAAGTGGCATTGCTGGCAGATTGGAT
CGCGAGTTTTAAAATCCACTATCCATCTGTGCCTGTGATTGTCGATCCTGTGATGGTCGCCAGTTGTGGCGATGCATTAG
CAGTAGATAACTGTCAGGATATAAAAAGTGCGGCTAAATCAGCCTTAGATTTTAAGCCTTTCAAAGGTTTAATCGAACTT
ATCACGCCCAATGTGCTTGAACTTGGGCGGTTAACTCACAGTGATGTTTCAACGAAAGCGCAATTCGCTGCCGCGGCACT
GGCCTTATCCCAGAGCCTTGATTGCAGTGTGCTCGCCAAAGGGGGCGATGTGAGCTTTGGCAGCACTGACATTCTTGATG
ATACTCATGCTCAAACTCACGATAACACTTATGCTCAAACTCAGGCTAACGTTCATGTTATCGCTCTTGATAGCAACGGC
TGGGACCTTGAGCTTGCCGAGGATTATCTAGTTTGTCGTCAAGTGCGCGCGAGCTCTAAACTACATCAAAATGGGCGTTT
CTGGTTAGCAAGCCAGCGGGTTAATACCCCTCATAACCATGGTAGCGGCTGTACTTTGTCATCGGCCATTGCCGCCGTGT
TAGCGCAGGGGTTTGTATTACAAGATGCGGTGGTGGTTGCCAAAGCCTACGTGAGTCAAGGCTTAAGCGCGGCGATTGGT
TTAGGGCAAGGTCCAGGGCCGTTGGCCCGTACGGGTTGGCCTAATGATGTGTCCCGCTATGCCAAGATAAATCTGTGTGA
TAGCAATTTTATTAGTCATCAACTCAACCAACACCTTGATGTTGGTAATGATTTAGTTGCAACAGTTTTATCCGCAACAG
ATCAGGCAACCGCTCAGGTAAGAATAGCCTCGACGCAACCTCAAAATATTTTATCCCACGGTTTTAAAGTGCTCGATGCC
GATCTTGGTGTTTACCCCGTAGTGAATGACTTAACCATGCTGGAGAGTTTGTTAGCTGCGGGCGTTAAAACCCTGCAGTT
ACGGATAAAAACCGACATCAGCGAGTTAACTACTGCAGGGTTAGCCGAATCTGATTTAGGTAAATCTGCGCTAAGTAGAT
GTGAGTCAGGCAAATCTAAGTCAGGCGAACCTGAGTTAATTGGCTCCGAATTAGAAGCACAAATTCAAACGGCCATTGCC
TTAGGTAAGCATTTTAATGCGCAGCTTTTTATCAATGATCACTGGCAGTTAGCGATAAAATACCATGCCTTTGGGGTACA
TTTAGGCCAAGAAGATCTCGCCGTTACCGACTTAGCGGCCATTCAAGCCGCGGGGCTCGCGCTAGGCATATCGAGCCACA
GTTATTTCGAGCTGTTATTGGCGCACCAATACTCGCCATCCTACATAGCGCTTGGGCATATATTCCCAACCACGACGAAG
CAAATGCCTTCGGCGCCCCAAGGGCTCGCAAAACTTAAACACTATGTGGCGTTACTCCAAGACCATTATCCCTTGGTCGC
CATTGGCGGTATTGACTTAACAAATCTGGCAAAGGTGAAAGCAACGGGGGTGGGCAATATTGCTGTGGTGCGCGCAATAA
CGAAAGCTAAGGATCCGTTAGCCGCCTTTGCAGAGTTGAGCCAAGCTTGGGAGCAATGTAGCTTGTCTGAAGAACTGGCT
GTAAAGCATGAGTTGGATGCAAAGCATGAGTAA

Upstream 100 bases:

>100_bases
AAGCGGCAATGGCGCTAAAATCGGCACAATTTGCTGCTTCGGGCGCCGAGATCTATCACGTGAAAGACAAGCTTGTCGCC
GAAGCTGAGGAAGCCTAATT

Downstream 100 bases:

>100_bases
AAACCCACCGCTTGCTCACGCAGAGGTTCTTTGCGGTCATATACACAGCGACAATGAACACTGCGGCCATACACTCAGTG
ATGCCGATTTCATCCGTTAT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MLGIHGDNAPMNTEHPAFVWTIAGSDSGGGAGIQADLATIQDLGCHGCSVVTTVTAQSSVAVTLVEPVSAAMLMAQLTTL
LSDLPPKAIKIGLLADQTQVALLADWIASFKIHYPSVPVIVDPVMVASCGDALAVDNCQDIKSAAKSALDFKPFKGLIEL
ITPNVLELGRLTHSDVSTKAQFAAAALALSQSLDCSVLAKGGDVSFGSTDILDDTHAQTHDNTYAQTQANVHVIALDSNG
WDLELAEDYLVCRQVRASSKLHQNGRFWLASQRVNTPHNHGSGCTLSSAIAAVLAQGFVLQDAVVVAKAYVSQGLSAAIG
LGQGPGPLARTGWPNDVSRYAKINLCDSNFISHQLNQHLDVGNDLVATVLSATDQATAQVRIASTQPQNILSHGFKVLDA
DLGVYPVVNDLTMLESLLAAGVKTLQLRIKTDISELTTAGLAESDLGKSALSRCESGKSKSGEPELIGSELEAQIQTAIA
LGKHFNAQLFINDHWQLAIKYHAFGVHLGQEDLAVTDLAAIQAAGLALGISSHSYFELLLAHQYSPSYIALGHIFPTTTK
QMPSAPQGLAKLKHYVALLQDHYPLVAIGGIDLTNLAKVKATGVGNIAVVRAITKAKDPLAAFAELSQAWEQCSLSEELA
VKHELDAKHE

Sequences:

>Translated_650_residues
MLGIHGDNAPMNTEHPAFVWTIAGSDSGGGAGIQADLATIQDLGCHGCSVVTTVTAQSSVAVTLVEPVSAAMLMAQLTTL
LSDLPPKAIKIGLLADQTQVALLADWIASFKIHYPSVPVIVDPVMVASCGDALAVDNCQDIKSAAKSALDFKPFKGLIEL
ITPNVLELGRLTHSDVSTKAQFAAAALALSQSLDCSVLAKGGDVSFGSTDILDDTHAQTHDNTYAQTQANVHVIALDSNG
WDLELAEDYLVCRQVRASSKLHQNGRFWLASQRVNTPHNHGSGCTLSSAIAAVLAQGFVLQDAVVVAKAYVSQGLSAAIG
LGQGPGPLARTGWPNDVSRYAKINLCDSNFISHQLNQHLDVGNDLVATVLSATDQATAQVRIASTQPQNILSHGFKVLDA
DLGVYPVVNDLTMLESLLAAGVKTLQLRIKTDISELTTAGLAESDLGKSALSRCESGKSKSGEPELIGSELEAQIQTAIA
LGKHFNAQLFINDHWQLAIKYHAFGVHLGQEDLAVTDLAAIQAAGLALGISSHSYFELLLAHQYSPSYIALGHIFPTTTK
QMPSAPQGLAKLKHYVALLQDHYPLVAIGGIDLTNLAKVKATGVGNIAVVRAITKAKDPLAAFAELSQAWEQCSLSEELA
VKHELDAKHE
>Mature_650_residues
MLGIHGDNAPMNTEHPAFVWTIAGSDSGGGAGIQADLATIQDLGCHGCSVVTTVTAQSSVAVTLVEPVSAAMLMAQLTTL
LSDLPPKAIKIGLLADQTQVALLADWIASFKIHYPSVPVIVDPVMVASCGDALAVDNCQDIKSAAKSALDFKPFKGLIEL
ITPNVLELGRLTHSDVSTKAQFAAAALALSQSLDCSVLAKGGDVSFGSTDILDDTHAQTHDNTYAQTQANVHVIALDSNG
WDLELAEDYLVCRQVRASSKLHQNGRFWLASQRVNTPHNHGSGCTLSSAIAAVLAQGFVLQDAVVVAKAYVSQGLSAAIG
LGQGPGPLARTGWPNDVSRYAKINLCDSNFISHQLNQHLDVGNDLVATVLSATDQATAQVRIASTQPQNILSHGFKVLDA
DLGVYPVVNDLTMLESLLAAGVKTLQLRIKTDISELTTAGLAESDLGKSALSRCESGKSKSGEPELIGSELEAQIQTAIA
LGKHFNAQLFINDHWQLAIKYHAFGVHLGQEDLAVTDLAAIQAAGLALGISSHSYFELLLAHQYSPSYIALGHIFPTTTK
QMPSAPQGLAKLKHYVALLQDHYPLVAIGGIDLTNLAKVKATGVGNIAVVRAITKAKDPLAAFAELSQAWEQCSLSEELA
VKHELDAKHE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=224, Percent_Identity=52.2321428571429, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788420, Length=314, Percent_Identity=29.9363057324841, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6325378, Length=305, Percent_Identity=28.1967213114754, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324997, Length=195, Percent_Identity=29.2307692307692, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324517, Length=203, Percent_Identity=29.5566502463054, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 68611; Mature: 68611

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGIHGDNAPMNTEHPAFVWTIAGSDSGGGAGIQADLATIQDLGCHGCSVVTTVTAQSSV
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCE
AVTLVEPVSAAMLMAQLTTLLSDLPPKAIKIGLLADQTQVALLADWIASFKIHYPSVPVI
EEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCEEECCCCCEE
VDPVMVASCGDALAVDNCQDIKSAAKSALDFKPFKGLIELITPNVLELGRLTHSDVSTKA
ECCHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHH
QFAAAALALSQSLDCSVLAKGGDVSFGSTDILDDTHAQTHDNTYAQTQANVHVIALDSNG
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCC
WDLELAEDYLVCRQVRASSKLHQNGRFWLASQRVNTPHNHGSGCTLSSAIAAVLAQGFVL
CEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHH
QDAVVVAKAYVSQGLSAAIGLGQGPGPLARTGWPNDVSRYAKINLCDSNFISHQLNQHLD
HHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCHHHHHEEEEEECCCHHHHHHHHHHCC
VGNDLVATVLSATDQATAQVRIASTQPQNILSHGFKVLDADLGVYPVVNDLTMLESLLAA
CCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHC
GVKTLQLRIKTDISELTTAGLAESDLGKSALSRCESGKSKSGEPELIGSELEAQIQTAIA
CCEEEEEEEECCHHHHHHCCCCHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LGKHFNAQLFINDHWQLAIKYHAFGVHLGQEDLAVTDLAAIQAAGLALGISSHSYFELLL
HCCCCCEEEEEECCEEEEEEEEEEEEEECCCCHHHHHHHHHHHCCEEEECCCCHHHHHHH
AHQYSPSYIALGHIFPTTTKQMPSAPQGLAKLKHYVALLQDHYPLVAIGGIDLTNLAKVK
HHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHEEE
ATGVGNIAVVRAITKAKDPLAAFAELSQAWEQCSLSEELAVKHELDAKHE
ECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MLGIHGDNAPMNTEHPAFVWTIAGSDSGGGAGIQADLATIQDLGCHGCSVVTTVTAQSSV
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCE
AVTLVEPVSAAMLMAQLTTLLSDLPPKAIKIGLLADQTQVALLADWIASFKIHYPSVPVI
EEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCEEECCCCCEE
VDPVMVASCGDALAVDNCQDIKSAAKSALDFKPFKGLIELITPNVLELGRLTHSDVSTKA
ECCHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHH
QFAAAALALSQSLDCSVLAKGGDVSFGSTDILDDTHAQTHDNTYAQTQANVHVIALDSNG
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCC
WDLELAEDYLVCRQVRASSKLHQNGRFWLASQRVNTPHNHGSGCTLSSAIAAVLAQGFVL
CEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHH
QDAVVVAKAYVSQGLSAAIGLGQGPGPLARTGWPNDVSRYAKINLCDSNFISHQLNQHLD
HHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCHHHHHEEEEEECCCHHHHHHHHHHCC
VGNDLVATVLSATDQATAQVRIASTQPQNILSHGFKVLDADLGVYPVVNDLTMLESLLAA
CCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHC
GVKTLQLRIKTDISELTTAGLAESDLGKSALSRCESGKSKSGEPELIGSELEAQIQTAIA
CCEEEEEEEECCHHHHHHCCCCHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LGKHFNAQLFINDHWQLAIKYHAFGVHLGQEDLAVTDLAAIQAAGLALGISSHSYFELLL
HCCCCCEEEEEECCEEEEEEEEEEEEEECCCCHHHHHHHHHHHCCEEEECCCCHHHHHHH
AHQYSPSYIALGHIFPTTTKQMPSAPQGLAKLKHYVALLQDHYPLVAIGGIDLTNLAKVK
HHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHEEE
ATGVGNIAVVRAITKAKDPLAAFAELSQAWEQCSLSEELAVKHELDAKHE
ECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA