Click here to switch to the map view.

The map label for this gene is thiC [H]

Identifier: 126174244

GI number: 126174244

Start: 2342318

End: 2342992

Strand: Direct

Name: thiC [H]

Synonym: Sbal_2022

Alternate gene names: 126174244

Gene position: 2342318-2342992 (Clockwise)

Preceding gene: 126174240

Following gene: 126174245

Centisome position: 45.68

GC content: 50.96

Gene sequence:

>675_bases
ATGCAGCTCATTAAAGAGCACATGGACAAGCAGTTAGCTCACTGCGATGAGGCGCCGTTTTACACGCTTGGCCCGCAAAT
CACCGATATCGCACCTGGGTATGATCATTTCACCTCCGGCATAGGCGCGGCCATGATTGCTTGGTACGGCTGCGCTATGT
TGTGTTATGTCACGCCAAAAGAACACTTAGGATTACCGAATAAACAAGATGTTAAGCAAGGTTTGATTGCTTATAAGATT
GCCGCCCACGCCGCCGATGTGGCGAAAGGTCATCCGAGTGCGCAAATTCGTGATAATGCGCTGGCAAAGGCGCGGTTTGA
ATTCCGCTGGGAAGATCAATATAACTTAGGACTCGATCCTGAAACCGCACGTGCTTATCACGATGAATCTTTACCGCAGG
AATCCGCTAAAGTCGCGCATTTTTGCTCTATGTGCGGCCCTAAATTTTGTTCGATGAAAATCACCCAAGATGTGCGCGCC
TATGCCGCAGGCTTAGAGGCGGCACAAGCGAAGGCGGATCAAGTTGAAGCTGCTGACCAAGCCATGCAAGTCACAGTCAA
GATGGCGCAGGAGTTGGAAGCGGCAATGGCGCTAAAATCGGCACAATTTGCTGCTTCGGGCGCCGAGATCTATCACGTGA
AAGACAAGCTTGTCGCCGAAGCTGAGGAAGCCTAA

Upstream 100 bases:

>100_bases
CACAAAGAACTCCCTTAGAATCTTTGTCCAGAATGTATCAGATCAACCGGCCATTCTAATTGTCGCTGAACCGGTCAGGG
TAATTGTCGCGCAATACCCG

Downstream 100 bases:

>100_bases
TTATGTTGGGTATTCATGGGGATAATGCGCCCATGAATACCGAGCATCCCGCGTTTGTTTGGACCATAGCTGGTTCAGAT
AGTGGCGGTGGTGCCGGTAT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MQLIKEHMDKQLAHCDEAPFYTLGPQITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPKEHLGLPNKQDVKQGLIAYKI
AAHAADVAKGHPSAQIRDNALAKARFEFRWEDQYNLGLDPETARAYHDESLPQESAKVAHFCSMCGPKFCSMKITQDVRA
YAAGLEAAQAKADQVEAADQAMQVTVKMAQELEAAMALKSAQFAASGAEIYHVKDKLVAEAEEA

Sequences:

>Translated_224_residues
MQLIKEHMDKQLAHCDEAPFYTLGPQITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPKEHLGLPNKQDVKQGLIAYKI
AAHAADVAKGHPSAQIRDNALAKARFEFRWEDQYNLGLDPETARAYHDESLPQESAKVAHFCSMCGPKFCSMKITQDVRA
YAAGLEAAQAKADQVEAADQAMQVTVKMAQELEAAMALKSAQFAASGAEIYHVKDKLVAEAEEA
>Mature_224_residues
MQLIKEHMDKQLAHCDEAPFYTLGPQITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPKEHLGLPNKQDVKQGLIAYKI
AAHAADVAKGHPSAQIRDNALAKARFEFRWEDQYNLGLDPETARAYHDESLPQESAKVAHFCSMCGPKFCSMKITQDVRA
YAAGLEAAQAKADQVEAADQAMQVTVKMAQELEAAMALKSAQFAASGAEIYHVKDKLVAEAEEA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=216, Percent_Identity=68.5185185185185, Blast_Score=317, Evalue=5e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 24465; Mature: 24465

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLIKEHMDKQLAHCDEAPFYTLGPQITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPK
CCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCH
EHLGLPNKQDVKQGLIAYKIAAHAADVAKGHPSAQIRDNALAKARFEFRWEDQYNLGLDP
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCC
ETARAYHDESLPQESAKVAHFCSMCGPKFCSMKITQDVRAYAAGLEAAQAKADQVEAADQ
HHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMQVTVKMAQELEAAMALKSAQFAASGAEIYHVKDKLVAEAEEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCC
>Mature Secondary Structure
MQLIKEHMDKQLAHCDEAPFYTLGPQITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPK
CCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCH
EHLGLPNKQDVKQGLIAYKIAAHAADVAKGHPSAQIRDNALAKARFEFRWEDQYNLGLDP
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCC
ETARAYHDESLPQESAKVAHFCSMCGPKFCSMKITQDVRAYAAGLEAAQAKADQVEAADQ
HHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMQVTVKMAQELEAAMALKSAQFAASGAEIYHVKDKLVAEAEEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA