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The map label for this gene is tcyJ [H]

Identifier: 125624394

GI number: 125624394

Start: 1566722

End: 1567570

Strand: Reverse

Name: tcyJ [H]

Synonym: llmg_1593

Alternate gene names: 125624394

Gene position: 1567570-1566722 (Counterclockwise)

Preceding gene: 125624395

Following gene: 125624393

Centisome position: 61.97

GC content: 35.34

Gene sequence:

>849_bases
ATGAGAACATCACTAAAGGTCACATTTGCTGCTCTTTCACTTATCGCTGCTGGAACTTTGGTTGCATGCTCCTCAGGTTC
AAAAAAGACGTCAGATAATAAAACAACATCAATTGTAATTGCAACAGATGGTGCAACAAAACCATTTACTTATTCAGATA
GTCAAGGAAATTTAACTGGTTATGATATTGAAGTCGCTCGTGCTGTCTTCAAAAAAATGCCGCAATACACTGTGAAATTT
CAAGCCACAGACTTTTCTGGAATTGCGCCAGGGATTGATTCCGGTCGTTACCAAATGGGTGCTAATGATTTTGGTTGGGA
AAAAAGCAGAGCTGAAAAATACTATTTCTCATCACCACTTTCCAAATCTAATAACGCTGTTCTTGTGAAATCTGGAACTT
ATAAAACCTTGGCAGATTTGGCTGGAAAATCAACAATTGGTAATCCAGCTTCAAATTATACAAAATCAATTCAAGATTGG
AACAAAGCCAATCCAGATAAACAAATTAAAATTAGTTACTCAGCAGATTCCACTTCTATTAATACTCGTTTCACACAAGT
AGAATCAGGTAAGATTGATTTCATGCTTTATGATAAAATTTCTTTACAGTCAGCTGTTAAAGAACAAGGATTTGATAATC
TAAAAATTGAAGATATCAGTATGGATACTGGCGATGCTGAACATGACGGTTATGAATATTTCCTTTTTGGTAAAGATAAA
GAAGGGCAAAAGCTTCAAAAAGATGTGAATGGAGTATTAGCTAAAATGCAAGCTGATGGTAGCTTGGCTAAGATTTCTAA
GAAATATCTTGGTGGAGATTTCGTCCCAAAAGCTGAGATGTTTAAATAG

Upstream 100 bases:

>100_bases
TGTTATTCTTATAGCCAAAAGTGGCTATAAGTTTTTTTTATGGCAAATTGGTTAAGTTCATGTTAAGATAGGTCTATCAA
ATTTTTAAAGGGGAACTTTT

Downstream 100 bases:

>100_bases
ATAGTTCTGACGAACAATCCTATTTTAATTAGCTCATTCTGTCAGTAAAATAAATGTTTTATAATTCATCAATTTACTGA
CAGCTCTGTTCGTAAAAAAT

Product: amino acid ABC transporter substrate binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRTSLKVTFAALSLIAAGTLVACSSGSKKTSDNKTTSIVIATDGATKPFTYSDSQGNLTGYDIEVARAVFKKMPQYTVKF
QATDFSGIAPGIDSGRYQMGANDFGWEKSRAEKYYFSSPLSKSNNAVLVKSGTYKTLADLAGKSTIGNPASNYTKSIQDW
NKANPDKQIKISYSADSTSINTRFTQVESGKIDFMLYDKISLQSAVKEQGFDNLKIEDISMDTGDAEHDGYEYFLFGKDK
EGQKLQKDVNGVLAKMQADGSLAKISKKYLGGDFVPKAEMFK

Sequences:

>Translated_282_residues
MRTSLKVTFAALSLIAAGTLVACSSGSKKTSDNKTTSIVIATDGATKPFTYSDSQGNLTGYDIEVARAVFKKMPQYTVKF
QATDFSGIAPGIDSGRYQMGANDFGWEKSRAEKYYFSSPLSKSNNAVLVKSGTYKTLADLAGKSTIGNPASNYTKSIQDW
NKANPDKQIKISYSADSTSINTRFTQVESGKIDFMLYDKISLQSAVKEQGFDNLKIEDISMDTGDAEHDGYEYFLFGKDK
EGQKLQKDVNGVLAKMQADGSLAKISKKYLGGDFVPKAEMFK
>Mature_282_residues
MRTSLKVTFAALSLIAAGTLVACSSGSKKTSDNKTTSIVIATDGATKPFTYSDSQGNLTGYDIEVARAVFKKMPQYTVKF
QATDFSGIAPGIDSGRYQMGANDFGWEKSRAEKYYFSSPLSKSNNAVLVKSGTYKTLADLAGKSTIGNPASNYTKSIQDW
NKANPDKQIKISYSADSTSINTRFTQVESGKIDFMLYDKISLQSAVKEQGFDNLKIEDISMDTGDAEHDGYEYFLFGKDK
EGQKLQKDVNGVLAKMQADGSLAKISKKYLGGDFVPKAEMFK

Specific function: Part of the ABC transporter complex tcyJKLMN involved in L-cystine import. Is also involved in cystathionine, djenkolate, and S-methylcysteine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=247, Percent_Identity=27.9352226720648, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787085, Length=223, Percent_Identity=25.1121076233184, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787031, Length=274, Percent_Identity=26.6423357664234, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30770; Mature: 30770

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSLKVTFAALSLIAAGTLVACSSGSKKTSDNKTTSIVIATDGATKPFTYSDSQGNLTG
CCCCEEHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCEEE
YDIEVARAVFKKMPQYTVKFQATDFSGIAPGIDSGRYQMGANDFGWEKSRAEKYYFSSPL
CHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHHCCCEEECCCC
SKSNNAVLVKSGTYKTLADLAGKSTIGNPASNYTKSIQDWNKANPDKQIKISYSADSTSI
CCCCCEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
NTRFTQVESGKIDFMLYDKISLQSAVKEQGFDNLKIEDISMDTGDAEHDGYEYFLFGKDK
CEEEEEEECCCEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEECCC
EGQKLQKDVNGVLAKMQADGSLAKISKKYLGGDFVPKAEMFK
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHCCC
>Mature Secondary Structure
MRTSLKVTFAALSLIAAGTLVACSSGSKKTSDNKTTSIVIATDGATKPFTYSDSQGNLTG
CCCCEEHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCEEE
YDIEVARAVFKKMPQYTVKFQATDFSGIAPGIDSGRYQMGANDFGWEKSRAEKYYFSSPL
CHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHHCCCEEECCCC
SKSNNAVLVKSGTYKTLADLAGKSTIGNPASNYTKSIQDWNKANPDKQIKISYSADSTSI
CCCCCEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
NTRFTQVESGKIDFMLYDKISLQSAVKEQGFDNLKIEDISMDTGDAEHDGYEYFLFGKDK
CEEEEEEECCCEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEECCC
EGQKLQKDVNGVLAKMQADGSLAKISKKYLGGDFVPKAEMFK
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]