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The map label for this gene is lepA [H]

Identifier: 125624271

GI number: 125624271

Start: 1436035

End: 1437858

Strand: Reverse

Name: lepA [H]

Synonym: llmg_1463

Alternate gene names: 125624271

Gene position: 1437858-1436035 (Counterclockwise)

Preceding gene: 125624272

Following gene: 125624270

Centisome position: 56.84

GC content: 39.8

Gene sequence:

>1824_bases
ATGAATTTACAAGAAATGAACGCGCGTAAGGAAAAAATTCGTAATTTTTCGATTATCGCCCATATTGACCACGGGAAATC
AACCCTTGCTGACCGTATTCTCGAGCAAACTGAAACTGTGTCAAAACGTGAAATGCAAGCCCAACTTTTAGACTCAATGG
ACCTTGAACGTGAACGTGGGATTACCATTAAATTAAATGCTATCGAGTTAAATTATAAAGCTAAAGATGGCGAAACTTAT
ATTTTCCACCTCATTGACACGCCAGGGCACGTCGATTTTACTTATGAAGTTTCACGAAGTCTTGCTGCCTGTGAAGGGGC
AATTTTAGTTGTTGATGCCGCCCAAGGAATCGAAGCTCAAACCCTCGCTAACGTTTATCTTGCTTTGGATAATGACCTCG
AAATTTTACCAGTCATCAATAAAATTGACTTACCAGCTGCTGACCCAGAAATGGTTCGCCAAGAAATTGAAGATGTGATT
GGTTTAGATGCTTCCGAAGCGGTTCTTGCCTCTGCTAAAGCTGGGATTGGGATTGAAGAAATTCTCGAACAAATCGTCGA
GAAAGTTCCAGCCCCTCAAGGTGAAGTTGACGCACCTCTCAAAGCTCTAATTTTTGACTCAGTTTATGATGCTTATCGAG
GAGTTATTCTTCAAATTCGTGTGATTGACGGTTCAGTCAAGGTTGGAGATCGTATTCAACTCATGAGTAACGGCAAAGAA
TTTGATGTTACAGAAGTTGGGATTTTCACACCAAAAGCAGTTTCCCGTGATTTCTTGATGGCCGGAGATGTTGGTTATGT
TGCAGCCGCAATCAAGACAGTTGCTGACACACGTGTTGGGGATACAGTGACACTCGCTTCAAATCCAGCGACCGAAGCCC
TAGAGGGTTATAAGGAAATGAACCCAATGGTGTTCGCTGGGATTTACCCAATTGAATCAAATAAATTTAACGACCTCCGT
GAGGCCCTTGAAAAACTTCAATTAAACGACGCCAGTCTTCGTTTTGAGCCTGAAACATCACAAGCTCTTGGTTTTGGATT
CCGTTGTGGTTTCCTTGGCCTCTTGCACATGGATGTCATTCAAGAGCGTTTAGAACGTGAATTTGGCATTGATTTGATTA
TGACTGCTCCATCCGTTGTTTATCATATTAATACTACAGATGGTGAAACATTAGAAGTTGCTAACCCATCGGAATTCCCA
GATCCTACACGCATTGAAAATATTGAAGAACCATTTGTCAAAGCTCAAATCATGGTGCCAAATGATTTCGTTGGTCCTGT
GATGGAATTGGCCCAACGAAAACGCGGGATTTTTCTAACAATGGATTATTTAGATGCAAATCGTGTCAATATCATTTATC
ATATTCCATTGAGTGAAATTGTCTTTGATTTCTTTGATAAACTTAAATCATCAACTAAAGGTTATGCCAGCTTTGATTAT
GAAATTTCTGATTATCGTCCATCAAATCTTGTAAAAATGGATATTCTCTTAAATGCTGAAAAAGTCGATGCCTTAAGTTT
CATTGTTCACAAAGACTTTGCCTTTGAACGCGGAAAAGTAATTGTTGAAAAACTTAAAAAACTCATTCCACGTCAACAAT
TTGAAGTTCCAATCCAAGCAACCATTGGAAACAAAATCGTTGCTCGTTCAGATATTAAAGCTCTTCGTAAAAACGTATTG
GCCAAATGTTATGGTGGAGATATTTCCCGTAAACGAAAACTCCTTGAAAAACAAAAAGCCGGTAAAAAACGGATGAAAGC
CATTGGTTCAGTTGAAGTCCCTCAAGAAGCCTTCCTCTCTGTCCTCTCAATGGACGAAGAATAA

Upstream 100 bases:

>100_bases
TTTTTAGTTATAAAAAAATTTAGCGCAAAAAAAGCGAAAAGAAAGTTCGTGGACGAAAAGCAAATGATAAGCCCACAAGA
ACTCTTATAAAGGAATCAAA

Downstream 100 bases:

>100_bases
AATAAAAAAAACCACCATTCAGGTGATTTTTTTTTATTTACTATTGTAAAAATCCTATTGACTATTTTATGTAACATATT
GTATAATAACAGAAATATAA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MNLQEMNARKEKIRNFSIIAHIDHGKSTLADRILEQTETVSKREMQAQLLDSMDLERERGITIKLNAIELNYKAKDGETY
IFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPEMVRQEIEDVI
GLDASEAVLASAKAGIGIEEILEQIVEKVPAPQGEVDAPLKALIFDSVYDAYRGVILQIRVIDGSVKVGDRIQLMSNGKE
FDVTEVGIFTPKAVSRDFLMAGDVGYVAAAIKTVADTRVGDTVTLASNPATEALEGYKEMNPMVFAGIYPIESNKFNDLR
EALEKLQLNDASLRFEPETSQALGFGFRCGFLGLLHMDVIQERLEREFGIDLIMTAPSVVYHINTTDGETLEVANPSEFP
DPTRIENIEEPFVKAQIMVPNDFVGPVMELAQRKRGIFLTMDYLDANRVNIIYHIPLSEIVFDFFDKLKSSTKGYASFDY
EISDYRPSNLVKMDILLNAEKVDALSFIVHKDFAFERGKVIVEKLKKLIPRQQFEVPIQATIGNKIVARSDIKALRKNVL
AKCYGGDISRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDEE

Sequences:

>Translated_607_residues
MNLQEMNARKEKIRNFSIIAHIDHGKSTLADRILEQTETVSKREMQAQLLDSMDLERERGITIKLNAIELNYKAKDGETY
IFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPEMVRQEIEDVI
GLDASEAVLASAKAGIGIEEILEQIVEKVPAPQGEVDAPLKALIFDSVYDAYRGVILQIRVIDGSVKVGDRIQLMSNGKE
FDVTEVGIFTPKAVSRDFLMAGDVGYVAAAIKTVADTRVGDTVTLASNPATEALEGYKEMNPMVFAGIYPIESNKFNDLR
EALEKLQLNDASLRFEPETSQALGFGFRCGFLGLLHMDVIQERLEREFGIDLIMTAPSVVYHINTTDGETLEVANPSEFP
DPTRIENIEEPFVKAQIMVPNDFVGPVMELAQRKRGIFLTMDYLDANRVNIIYHIPLSEIVFDFFDKLKSSTKGYASFDY
EISDYRPSNLVKMDILLNAEKVDALSFIVHKDFAFERGKVIVEKLKKLIPRQQFEVPIQATIGNKIVARSDIKALRKNVL
AKCYGGDISRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDEE
>Mature_607_residues
MNLQEMNARKEKIRNFSIIAHIDHGKSTLADRILEQTETVSKREMQAQLLDSMDLERERGITIKLNAIELNYKAKDGETY
IFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPEMVRQEIEDVI
GLDASEAVLASAKAGIGIEEILEQIVEKVPAPQGEVDAPLKALIFDSVYDAYRGVILQIRVIDGSVKVGDRIQLMSNGKE
FDVTEVGIFTPKAVSRDFLMAGDVGYVAAAIKTVADTRVGDTVTLASNPATEALEGYKEMNPMVFAGIYPIESNKFNDLR
EALEKLQLNDASLRFEPETSQALGFGFRCGFLGLLHMDVIQERLEREFGIDLIMTAPSVVYHINTTDGETLEVANPSEFP
DPTRIENIEEPFVKAQIMVPNDFVGPVMELAQRKRGIFLTMDYLDANRVNIIYHIPLSEIVFDFFDKLKSSTKGYASFDY
EISDYRPSNLVKMDILLNAEKVDALSFIVHKDFAFERGKVIVEKLKKLIPRQQFEVPIQATIGNKIVARSDIKALRKNVL
AKCYGGDISRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDEE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=600, Percent_Identity=49.3333333333333, Blast_Score=612, Evalue=1e-175,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=45.4545454545455, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI18390331, Length=161, Percent_Identity=37.888198757764, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI4503483, Length=147, Percent_Identity=38.7755102040816, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI25306283, Length=141, Percent_Identity=42.5531914893617, Blast_Score=100, Evalue=8e-21,
Organism=Homo sapiens, GI19923640, Length=135, Percent_Identity=43.7037037037037, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI25306287, Length=135, Percent_Identity=43.7037037037037, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI310132016, Length=117, Percent_Identity=40.1709401709402, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310110807, Length=117, Percent_Identity=40.1709401709402, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310123363, Length=117, Percent_Identity=40.1709401709402, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI217272894, Length=170, Percent_Identity=32.3529411764706, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI217272892, Length=170, Percent_Identity=32.3529411764706, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI53729339, Length=252, Percent_Identity=26.984126984127, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI53729337, Length=252, Percent_Identity=26.984126984127, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI34147630, Length=269, Percent_Identity=26.3940520446097, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=38.1443298969072, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=57.286432160804, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=550, Percent_Identity=30.5454545454545, Blast_Score=179, Evalue=7e-46,
Organism=Escherichia coli, GI1789738, Length=183, Percent_Identity=34.9726775956284, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790835, Length=163, Percent_Identity=33.1288343558282, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789559, Length=256, Percent_Identity=29.6875, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790412, Length=249, Percent_Identity=26.5060240963855, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789737, Length=249, Percent_Identity=26.5060240963855, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789108, Length=288, Percent_Identity=26.7361111111111, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=622, Percent_Identity=42.604501607717, Blast_Score=520, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=39.7260273972603, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=161, Percent_Identity=34.7826086956522, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=40, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=40, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=159, Percent_Identity=33.9622641509434, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=32.4137931034483, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=254, Percent_Identity=29.9212598425197, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=285, Percent_Identity=25.9649122807018, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=612, Percent_Identity=44.9346405228758, Blast_Score=531, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6323098, Length=186, Percent_Identity=37.6344086021505, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=39.8601398601399, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=39.8601398601399, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=113, Percent_Identity=40.7079646017699, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=153, Percent_Identity=34.640522875817, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=273, Percent_Identity=27.4725274725275, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322675, Length=141, Percent_Identity=28.3687943262411, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=600, Percent_Identity=44.5, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24582462, Length=185, Percent_Identity=37.2972972972973, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28574573, Length=224, Percent_Identity=33.4821428571429, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585709, Length=151, Percent_Identity=33.7748344370861, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=33.7748344370861, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=33.7748344370861, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI221458488, Length=150, Percent_Identity=38.6666666666667, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21357743, Length=162, Percent_Identity=32.7160493827161, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281363316, Length=258, Percent_Identity=29.0697674418605, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17864358, Length=258, Percent_Identity=29.0697674418605, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19921738, Length=233, Percent_Identity=30.9012875536481, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67700; Mature: 67700

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQEMNARKEKIRNFSIIAHIDHGKSTLADRILEQTETVSKREMQAQLLDSMDLERERG
CCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
ITIKLNAIELNYKAKDGETYIFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQ
EEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHH
TLANVYLALDNDLEILPVINKIDLPAADPEMVRQEIEDVIGLDASEAVLASAKAGIGIEE
HHEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
ILEQIVEKVPAPQGEVDAPLKALIFDSVYDAYRGVILQIRVIDGSVKVGDRIQLMSNGKE
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEEECCCCC
FDVTEVGIFTPKAVSRDFLMAGDVGYVAAAIKTVADTRVGDTVTLASNPATEALEGYKEM
CCEEEEEEECCHHHCCCCEEECCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHC
NPMVFAGIYPIESNKFNDLREALEKLQLNDASLRFEPETSQALGFGFRCGFLGLLHMDVI
CCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCHHHCCCHHHHHHHHHHHHHH
QERLEREFGIDLIMTAPSVVYHINTTDGETLEVANPSEFPDPTRIENIEEPFVKAQIMVP
HHHHHHHHCCEEEEECCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECC
NDFVGPVMELAQRKRGIFLTMDYLDANRVNIIYHIPLSEIVFDFFDKLKSSTKGYASFDY
CCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEE
EISDYRPSNLVKMDILLNAEKVDALSFIVHKDFAFERGKVIVEKLKKLIPRQQFEVPIQA
EECCCCCCCEEEEEEEECCHHHHHHHHHHHHCCHHHCHHHHHHHHHHHCCCCCCCCCEEE
TIGNKIVARSDIKALRKNVLAKCYGGDISRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLS
ECCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VLSMDEE
HHHCCCC
>Mature Secondary Structure
MNLQEMNARKEKIRNFSIIAHIDHGKSTLADRILEQTETVSKREMQAQLLDSMDLERERG
CCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
ITIKLNAIELNYKAKDGETYIFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQ
EEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHH
TLANVYLALDNDLEILPVINKIDLPAADPEMVRQEIEDVIGLDASEAVLASAKAGIGIEE
HHEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
ILEQIVEKVPAPQGEVDAPLKALIFDSVYDAYRGVILQIRVIDGSVKVGDRIQLMSNGKE
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEEECCCCC
FDVTEVGIFTPKAVSRDFLMAGDVGYVAAAIKTVADTRVGDTVTLASNPATEALEGYKEM
CCEEEEEEECCHHHCCCCEEECCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHC
NPMVFAGIYPIESNKFNDLREALEKLQLNDASLRFEPETSQALGFGFRCGFLGLLHMDVI
CCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCHHHCCCHHHHHHHHHHHHHH
QERLEREFGIDLIMTAPSVVYHINTTDGETLEVANPSEFPDPTRIENIEEPFVKAQIMVP
HHHHHHHHCCEEEEECCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECC
NDFVGPVMELAQRKRGIFLTMDYLDANRVNIIYHIPLSEIVFDFFDKLKSSTKGYASFDY
CCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEE
EISDYRPSNLVKMDILLNAEKVDALSFIVHKDFAFERGKVIVEKLKKLIPRQQFEVPIQA
EECCCCCCCEEEEEEEECCHHHHHHHHHHHHCCHHHCHHHHHHHHHHHCCCCCCCCCEEE
TIGNKIVARSDIKALRKNVLAKCYGGDISRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLS
ECCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VLSMDEE
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]