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The map label for this gene is pbpE [H]

Identifier: 124485644

GI number: 124485644

Start: 799401

End: 800768

Strand: Reverse

Name: pbpE [H]

Synonym: Mlab_0822

Alternate gene names: 124485644

Gene position: 800768-799401 (Counterclockwise)

Preceding gene: 124485645

Following gene: 124485643

Centisome position: 44.36

GC content: 55.26

Gene sequence:

>1368_bases
ATGGCGGTCGCGATTATTCAAAACGATACGGTCGTCTACGAAAAATGCTTCGGCGTGATCAATCTCACGACGCAGGAGCC
GGTAAATGCCGATACGCGGTTCCAGCTCGCCTCCATCTCGAAGTCCTTTACGACCGCGACGATTGCGTCGATGGTCGGGA
CCGGCGATCTTGCATGGGATGATTCGGCCGTGGAAATCAATCCTGACCTTCAGTTTTCGGATCCATGGATCACGGAACAT
GTGACCATTCGCGATCTTCTTTCGCACCGGACGGGACTTCCCGAGTATGCCGGCGACGAGCTGGCCGAGTTCGGATACAA
CCGTTCGGAAACCCTCTTTAAACTCAGATATCTTGCACTGACGGGCGCGTTCCGCTCGAGTTACGCCTACTCGAATCTCG
GGATCACTGCAGCCGCCGAAACTGCGGCGATAAAAGCCGGAATTCCTTGGGATGAACTGATTTCTGAGAGGATCTTCATC
CCGGCAGGTATGACGAACTCGAGTGCGGTCTTTTCCGACTTTGCCGAGGCCGAGAACCATGCCGACACCTATGTCGTGAC
GAACGGCGTTGCGGAACAAACCGCAGTTCTGTTCAACGATGATGCGAACAGTCCGGCCGGGGGAGTCAGTTCGACGCTGA
ACGATATGATCAGATATCTCCGCCTGCAGTTGAACGACGGAAATCTCGACGGCGTGCAGGTGATCGATGCCTCTGCGCTG
AGCGAGACCCACAAAGCCCAGAACATCCTGCTCTCCGACTATACCACCATCTCGGCATACGGTCTTGGATGGGACGTGGT
ATCTGCGGACGGCAGGGTCAGGGTCGAACACGGCGGCGACTTTACGAGTGGTGTCTCCACGCTGATCACCCTCTACCCGG
CAGAAGGGATGGGCATCGTTGTTTTAACGAACGGCTTTCCTGGAGGGCATGTCCTGAAAAAAGCCGTTGTGCGGGGCTGG
GAAGATATGTACTTCACGGGTTCCGTGCAGAATGACTGGTACGCGGAGATCGAGGAGGAGATCAATGCAGCCATGGAGCC
GGGATCTTCGGTGCTGAGTCCATATTCAACGCTTCCAGAGGCTCCGGAAAATGCGTCATCGCCCCGGGATCTGGTAAACT
ACTGCGGATCGTATTATCAGGATTATTACGGTACGATAACGGTCGAAGAGGGAGACAGCGGCCTTAATCTGTATCTCGGA
CCGGCCGAAATGCAGTACGTCCTTACGCCGTATGACGGGGATGCCTTTTACGAGCCGACGACCGGCACCGGTGTCTACTT
CACCTTCGATAGTGACGAGAACGTGCAGAGCGTGAATATTACGATGCTTGATCTTCCGGGAAGGACGGGCGTGTTTACGC
TGATTTAG

Upstream 100 bases:

>100_bases
GGCCTATGACCAGACGCCGGCGGCAGGAGATCTCGATAAAACGATTCCTGAGTTCGATCTCTACACAGAAGAACTCTTCA
ACAAAAGCGGCATCCCGGGC

Downstream 100 bases:

>100_bases
ACCGGGGCAATCTTTTTTTCAAGAGGCAACGATACCTTCAACAACCATGCTGCAGAAAATACTCCGAATCTTCGCCGATC
CACCCCAGCCGGCAGACGCC

Product: 6Fe-6S prismane cluster-containing protein-like protein

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAVAIIQNDTVVYEKCFGVINLTTQEPVNADTRFQLASISKSFTTATIASMVGTGDLAWDDSAVEINPDLQFSDPWITEH
VTIRDLLSHRTGLPEYAGDELAEFGYNRSETLFKLRYLALTGAFRSSYAYSNLGITAAAETAAIKAGIPWDELISERIFI
PAGMTNSSAVFSDFAEAENHADTYVVTNGVAEQTAVLFNDDANSPAGGVSSTLNDMIRYLRLQLNDGNLDGVQVIDASAL
SETHKAQNILLSDYTTISAYGLGWDVVSADGRVRVEHGGDFTSGVSTLITLYPAEGMGIVVLTNGFPGGHVLKKAVVRGW
EDMYFTGSVQNDWYAEIEEEINAAMEPGSSVLSPYSTLPEAPENASSPRDLVNYCGSYYQDYYGTITVEEGDSGLNLYLG
PAEMQYVLTPYDGDAFYEPTTGTGVYFTFDSDENVQSVNITMLDLPGRTGVFTLI

Sequences:

>Translated_455_residues
MAVAIIQNDTVVYEKCFGVINLTTQEPVNADTRFQLASISKSFTTATIASMVGTGDLAWDDSAVEINPDLQFSDPWITEH
VTIRDLLSHRTGLPEYAGDELAEFGYNRSETLFKLRYLALTGAFRSSYAYSNLGITAAAETAAIKAGIPWDELISERIFI
PAGMTNSSAVFSDFAEAENHADTYVVTNGVAEQTAVLFNDDANSPAGGVSSTLNDMIRYLRLQLNDGNLDGVQVIDASAL
SETHKAQNILLSDYTTISAYGLGWDVVSADGRVRVEHGGDFTSGVSTLITLYPAEGMGIVVLTNGFPGGHVLKKAVVRGW
EDMYFTGSVQNDWYAEIEEEINAAMEPGSSVLSPYSTLPEAPENASSPRDLVNYCGSYYQDYYGTITVEEGDSGLNLYLG
PAEMQYVLTPYDGDAFYEPTTGTGVYFTFDSDENVQSVNITMLDLPGRTGVFTLI
>Mature_454_residues
AVAIIQNDTVVYEKCFGVINLTTQEPVNADTRFQLASISKSFTTATIASMVGTGDLAWDDSAVEINPDLQFSDPWITEHV
TIRDLLSHRTGLPEYAGDELAEFGYNRSETLFKLRYLALTGAFRSSYAYSNLGITAAAETAAIKAGIPWDELISERIFIP
AGMTNSSAVFSDFAEAENHADTYVVTNGVAEQTAVLFNDDANSPAGGVSSTLNDMIRYLRLQLNDGNLDGVQVIDASALS
ETHKAQNILLSDYTTISAYGLGWDVVSADGRVRVEHGGDFTSGVSTLITLYPAEGMGIVVLTNGFPGGHVLKKAVVRGWE
DMYFTGSVQNDWYAEIEEEINAAMEPGSSVLSPYSTLPEAPENASSPRDLVNYCGSYYQDYYGTITVEEGDSGLNLYLGP
AEMQYVLTPYDGDAFYEPTTGTGVYFTFDSDENVQSVNITMLDLPGRTGVFTLI

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 49348; Mature: 49217

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAIIQNDTVVYEKCFGVINLTTQEPVNADTRFQLASISKSFTTATIASMVGTGDLAWD
CEEEEEECCEEEEEECCCEEEECCCCCCCCCCEEEEEHHCCHHHHHHHHHHHCCCCCCCC
DSAVEINPDLQFSDPWITEHVTIRDLLSHRTGLPEYAGDELAEFGYNRSETLFKLRYLAL
CCEEEECCCCEECCCCCCCEEEHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHEEEEHHH
TGAFRSSYAYSNLGITAAAETAAIKAGIPWDELISERIFIPAGMTNSSAVFSDFAEAENH
HHHHHHCCEECCCCEEEECCCEEEECCCCHHHHHHCCEEEECCCCCCHHHHHHHHHHCCC
ADTYVVTNGVAEQTAVLFNDDANSPAGGVSSTLNDMIRYLRLQLNDGNLDGVQVIDASAL
CCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCEEEEEHHHH
SETHKAQNILLSDYTTISAYGLGWDVVSADGRVRVEHGGDFTSGVSTLITLYPAEGMGIV
HHHHHHHEEEEECCEEEEEECCCEEEEECCCEEEEECCCCHHHHHEEEEEEECCCCCEEE
VLTNGFPGGHVLKKAVVRGWEDMYFTGSVQNDWYAEIEEEINAAMEPGSSVLSPYSTLPE
EEECCCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCCHHHCCC
APENASSPRDLVNYCGSYYQDYYGTITVEEGDSGLNLYLGPAEMQYVLTPYDGDAFYEPT
CCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCEEEECCCCEEEEEEECCCCCEECCC
TGTGVYFTFDSDENVQSVNITMLDLPGRTGVFTLI
CCCEEEEEECCCCCEEEEEEEEEECCCCCCEEEEC
>Mature Secondary Structure 
AVAIIQNDTVVYEKCFGVINLTTQEPVNADTRFQLASISKSFTTATIASMVGTGDLAWD
EEEEEECCEEEEEECCCEEEECCCCCCCCCCEEEEEHHCCHHHHHHHHHHHCCCCCCCC
DSAVEINPDLQFSDPWITEHVTIRDLLSHRTGLPEYAGDELAEFGYNRSETLFKLRYLAL
CCEEEECCCCEECCCCCCCEEEHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHEEEEHHH
TGAFRSSYAYSNLGITAAAETAAIKAGIPWDELISERIFIPAGMTNSSAVFSDFAEAENH
HHHHHHCCEECCCCEEEECCCEEEECCCCHHHHHHCCEEEECCCCCCHHHHHHHHHHCCC
ADTYVVTNGVAEQTAVLFNDDANSPAGGVSSTLNDMIRYLRLQLNDGNLDGVQVIDASAL
CCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCEEEEEHHHH
SETHKAQNILLSDYTTISAYGLGWDVVSADGRVRVEHGGDFTSGVSTLITLYPAEGMGIV
HHHHHHHEEEEECCEEEEEECCCEEEEECCCEEEEECCCCHHHHHEEEEEEECCCCCEEE
VLTNGFPGGHVLKKAVVRGWEDMYFTGSVQNDWYAEIEEEINAAMEPGSSVLSPYSTLPE
EEECCCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCCHHHCCC
APENASSPRDLVNYCGSYYQDYYGTITVEEGDSGLNLYLGPAEMQYVLTPYDGDAFYEPT
CCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCEEEECCCCEEEEEEECCCCCEECCC
TGTGVYFTFDSDENVQSVNITMLDLPGRTGVFTLI
CCCEEEEEECCCCCEEEEEEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]