Click here to switch to the map view.

The map label for this gene is thsB [H]

Identifier: 124485593

GI number: 124485593

Start: 752388

End: 753974

Strand: Reverse

Name: thsB [H]

Synonym: Mlab_0771

Alternate gene names: 124485593

Gene position: 753974-752388 (Counterclockwise)

Preceding gene: 124485594

Following gene: 124485592

Centisome position: 41.77

GC content: 51.98

Gene sequence:

>1587_bases
ATGGCAAACAACTCAGGTCAGCAGGTCGTCATTTTACGTAATAACGTAGAACGCGTTCCGGGACAGGAAGCACTCCGCTC
CAATATCATGGCAGCAAAGGTCCTTGGCAATACAGTGAGAACAACTCTTGGCCCCCGCGGTATGGACAAGATGATGGTTA
CCCAGGGTAGCGACGATATCGTCATCACCAACGACGGAGCAACAATCCTGCACCAGATCCATGTTGAACACCCGGGTGCA
AAACTTGTCGTCGAAGTAGCTGAAACCCAGGACAATGAATGCGGCGACGGCACTACGACCGCAGTTGTGATGGTCGGTTC
GTTTATGGAGCAGGCAGAACACCTGATCGACAGCGGTGTCCACCCATCTGTTATCGCAAAAGGATACAACCTTGGTATGC
TCAAGGCGCTCGAACTCCTCGAAAAACTTGCGATCGACGTCACGCCGAAAGACAAAAACATGCTGAAACTTATCGCCAAA
ACCGCGATGACCGGAAAATCCATCGAAAGCATCATGGATCAGGCATGCGATGTTGTTGTCGAGGCAGTTTCCGGTGTTGC
AGAAACCTCCGGGAAAAAGACGCAGGTCAATGAAGATGACATCCTTGTCAAGACAAAACGCAGCGACACGATGAGTGCCG
AACTCATCAAAGGTGTCCTGATTGATAAAACCAGACTTGACTCACTGATGCCGAAAAAGATCAAAGGCGTCAAAGCAGCC
TTCATTGCCGGCCCGCTTGAGATCACCAAGACCCAGACCAAATCCAAGATCAAGATCACTTCAGCAGAACAGCTCTCGGC
ATTTTCCTCTGCAGAACGTGCGACCCTCAAGGCAATGGCAGACAAGTTTGCTGCGAACGGCGTAAATGTCGTCCTCTGCC
AGAAAGGAATCGCCGACCCGGTACAGTATTACCTTGGCGAACACGGCATCTATGCACTTGAGTTCGTTCCGGAAAAAGAT
ATGAAGTATGCAGCAAAAGCACTTGGCGGTCAGGTCATCAACAAACCCGAAGACCTCGCCCCCGAAACTATTGGAACTGC
AGGCAAACTCGAGATGCTCGAAGATCTCGAGATGACGAAACTCTCCGAGTGCAAAAACCCGAAGGCAGTGACCATCCTTC
TCCGCGGCTCCTCACAGCACCTTGTCGATGAACTCGAACGTGCGATCGAAGATGCAAAACGTGTCGTGCAGGACGTCGTC
GAAGATGGTTCCTACACAATCGGCGGCGGTTCTGTTGAGACCGAACTGTCCCTCAGACTCAGGGAATACGCAGCAACCGA
AGGCGGCCGTGTCCAGCTCGCCATCGAAGGCTATGCAAAAGCATTCGAGATCATCCCGAAGACTCTTGCAGAAAACTCCG
GCTTCGACACCGTTGACAAAGTCATCGATCTCCGTCAGGCACACGCGATGGGCCAGAAGTATGCAGGTCTTGATGTGTTT
ACCGGCACCGTTGTCGACATGAAGGAACTCGGCGTTGTTGAACCGAAGAGAGTTAAACGCCAGGCAATTCAGAGTGCCTC
GGAGACCGCCATGCTTCTCATCCGCGTTGATGACATGATGATCTCCAAAGGCGCCGGCCAGATGTAA

Upstream 100 bases:

>100_bases
CAATTAAACGTATCGACTGAAGAGTCAATAACCAATACAACATATTTATATAGAAGTCCAATCCACTTTTTTAACATATT
AGTACAGGAGAATAACGATT

Downstream 100 bases:

>100_bases
ATCCGGCCACCAATCCCATATATTTTTTACATTCAAACACGTATGCTTACTACGCGCATACCATAACGTATCGTATAGAC
ACCTGCCGAGGTAGTCTAGT

Product: hypothetical protein

Products: NA

Alternate protein names: Chaperonin subunit beta; Thermosome subunit 2 [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MANNSGQQVVILRNNVERVPGQEALRSNIMAAKVLGNTVRTTLGPRGMDKMMVTQGSDDIVITNDGATILHQIHVEHPGA
KLVVEVAETQDNECGDGTTTAVVMVGSFMEQAEHLIDSGVHPSVIAKGYNLGMLKALELLEKLAIDVTPKDKNMLKLIAK
TAMTGKSIESIMDQACDVVVEAVSGVAETSGKKTQVNEDDILVKTKRSDTMSAELIKGVLIDKTRLDSLMPKKIKGVKAA
FIAGPLEITKTQTKSKIKITSAEQLSAFSSAERATLKAMADKFAANGVNVVLCQKGIADPVQYYLGEHGIYALEFVPEKD
MKYAAKALGGQVINKPEDLAPETIGTAGKLEMLEDLEMTKLSECKNPKAVTILLRGSSQHLVDELERAIEDAKRVVQDVV
EDGSYTIGGGSVETELSLRLREYAATEGGRVQLAIEGYAKAFEIIPKTLAENSGFDTVDKVIDLRQAHAMGQKYAGLDVF
TGTVVDMKELGVVEPKRVKRQAIQSASETAMLLIRVDDMMISKGAGQM

Sequences:

>Translated_528_residues
MANNSGQQVVILRNNVERVPGQEALRSNIMAAKVLGNTVRTTLGPRGMDKMMVTQGSDDIVITNDGATILHQIHVEHPGA
KLVVEVAETQDNECGDGTTTAVVMVGSFMEQAEHLIDSGVHPSVIAKGYNLGMLKALELLEKLAIDVTPKDKNMLKLIAK
TAMTGKSIESIMDQACDVVVEAVSGVAETSGKKTQVNEDDILVKTKRSDTMSAELIKGVLIDKTRLDSLMPKKIKGVKAA
FIAGPLEITKTQTKSKIKITSAEQLSAFSSAERATLKAMADKFAANGVNVVLCQKGIADPVQYYLGEHGIYALEFVPEKD
MKYAAKALGGQVINKPEDLAPETIGTAGKLEMLEDLEMTKLSECKNPKAVTILLRGSSQHLVDELERAIEDAKRVVQDVV
EDGSYTIGGGSVETELSLRLREYAATEGGRVQLAIEGYAKAFEIIPKTLAENSGFDTVDKVIDLRQAHAMGQKYAGLDVF
TGTVVDMKELGVVEPKRVKRQAIQSASETAMLLIRVDDMMISKGAGQM
>Mature_527_residues
ANNSGQQVVILRNNVERVPGQEALRSNIMAAKVLGNTVRTTLGPRGMDKMMVTQGSDDIVITNDGATILHQIHVEHPGAK
LVVEVAETQDNECGDGTTTAVVMVGSFMEQAEHLIDSGVHPSVIAKGYNLGMLKALELLEKLAIDVTPKDKNMLKLIAKT
AMTGKSIESIMDQACDVVVEAVSGVAETSGKKTQVNEDDILVKTKRSDTMSAELIKGVLIDKTRLDSLMPKKIKGVKAAF
IAGPLEITKTQTKSKIKITSAEQLSAFSSAERATLKAMADKFAANGVNVVLCQKGIADPVQYYLGEHGIYALEFVPEKDM
KYAAKALGGQVINKPEDLAPETIGTAGKLEMLEDLEMTKLSECKNPKAVTILLRGSSQHLVDELERAIEDAKRVVQDVVE
DGSYTIGGGSVETELSLRLREYAATEGGRVQLAIEGYAKAFEIIPKTLAENSGFDTVDKVIDLRQAHAMGQKYAGLDVFT
GTVVDMKELGVVEPKRVKRQAIQSASETAMLLIRVDDMMISKGAGQM

Specific function: Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TCP-1 chaperonin family [H]

Homologues:

Organism=Homo sapiens, GI63162572, Length=523, Percent_Identity=34.7992351816444, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI24307939, Length=510, Percent_Identity=34.9019607843137, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI57863257, Length=524, Percent_Identity=35.3053435114504, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI38455427, Length=507, Percent_Identity=32.3471400394477, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI5453607, Length=531, Percent_Identity=32.5800376647834, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI58761484, Length=460, Percent_Identity=33.9130434782609, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI4502643, Length=525, Percent_Identity=31.047619047619, Blast_Score=251, Evalue=2e-66,
Organism=Homo sapiens, GI58331173, Length=505, Percent_Identity=32.2772277227723, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI261399877, Length=492, Percent_Identity=31.5040650406504, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI5453603, Length=527, Percent_Identity=30.3605313092979, Blast_Score=231, Evalue=2e-60,
Organism=Homo sapiens, GI302058290, Length=498, Percent_Identity=31.1244979919679, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI48762932, Length=513, Percent_Identity=30.9941520467836, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI261399875, Length=443, Percent_Identity=29.7968397291196, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI58331171, Length=525, Percent_Identity=27.6190476190476, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI302058292, Length=505, Percent_Identity=28.7128712871287, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI57863259, Length=377, Percent_Identity=33.9522546419098, Blast_Score=169, Evalue=7e-42,
Organism=Homo sapiens, GI58331185, Length=318, Percent_Identity=31.4465408805031, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI7657253, Length=507, Percent_Identity=24.2603550295858, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI41399285, Length=526, Percent_Identity=23.9543726235741, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI31542947, Length=526, Percent_Identity=23.9543726235741, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144674, Length=534, Percent_Identity=36.3295880149813, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17532603, Length=520, Percent_Identity=34.0384615384615, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17532601, Length=523, Percent_Identity=34.4168260038241, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17564182, Length=517, Percent_Identity=31.3346228239845, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI25148561, Length=533, Percent_Identity=29.8311444652908, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI25144678, Length=514, Percent_Identity=31.5175097276265, Blast_Score=244, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI25147750, Length=522, Percent_Identity=28.9272030651341, Blast_Score=228, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI71998178, Length=551, Percent_Identity=27.7676950998185, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI25144680, Length=396, Percent_Identity=31.0606060606061, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI32566944, Length=444, Percent_Identity=29.2792792792793, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71981457, Length=314, Percent_Identity=33.7579617834395, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322446, Length=525, Percent_Identity=32.5714285714286, Blast_Score=292, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6322524, Length=536, Percent_Identity=33.3955223880597, Blast_Score=282, Evalue=7e-77,
Organism=Saccharomyces cerevisiae, GI6322350, Length=536, Percent_Identity=31.1567164179104, Blast_Score=253, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6320418, Length=546, Percent_Identity=32.2344322344322, Blast_Score=249, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6322049, Length=521, Percent_Identity=32.0537428023033, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6320058, Length=513, Percent_Identity=29.8245614035088, Blast_Score=237, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6320393, Length=533, Percent_Identity=29.2682926829268, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322452, Length=524, Percent_Identity=28.4351145038168, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI17647245, Length=536, Percent_Identity=35.634328358209, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24652903, Length=510, Percent_Identity=36.078431372549, Blast_Score=295, Evalue=8e-80,
Organism=Drosophila melanogaster, GI24645179, Length=530, Percent_Identity=32.8301886792453, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24649027, Length=522, Percent_Identity=34.2911877394636, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24649029, Length=522, Percent_Identity=34.2911877394636, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24647512, Length=533, Percent_Identity=33.2082551594747, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24647510, Length=533, Percent_Identity=33.2082551594747, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24583944, Length=506, Percent_Identity=31.6205533596838, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI28571140, Length=520, Percent_Identity=30, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI18858175, Length=520, Percent_Identity=30, Blast_Score=241, Evalue=9e-64,
Organism=Drosophila melanogaster, GI18859933, Length=525, Percent_Identity=32, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI19921848, Length=517, Percent_Identity=29.9806576402321, Blast_Score=208, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24641193, Length=524, Percent_Identity=25.381679389313, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24641191, Length=524, Percent_Identity=25.381679389313, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45550936, Length=563, Percent_Identity=24.5115452930728, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550132, Length=563, Percent_Identity=24.5115452930728, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550935, Length=563, Percent_Identity=24.5115452930728, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17864606, Length=539, Percent_Identity=23.9332096474954, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017998
- InterPro:   IPR002194
- InterPro:   IPR002423
- InterPro:   IPR012714 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 56887; Mature: 56756

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00750 TCP1_1 ; PS00751 TCP1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNSGQQVVILRNNVERVPGQEALRSNIMAAKVLGNTVRTTLGPRGMDKMMVTQGSDDI
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCE
VITNDGATILHQIHVEHPGAKLVVEVAETQDNECGDGTTTAVVMVGSFMEQAEHLIDSGV
EEECCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
HPSVIAKGYNLGMLKALELLEKLAIDVTPKDKNMLKLIAKTAMTGKSIESIMDQACDVVV
CHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
EAVSGVAETSGKKTQVNEDDILVKTKRSDTMSAELIKGVLIDKTRLDSLMPKKIKGVKAA
HHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHHCHHHHCCCHHH
FIAGPLEITKTQTKSKIKITSAEQLSAFSSAERATLKAMADKFAANGVNVVLCQKGIADP
EEECCCCEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
VQYYLGEHGIYALEFVPEKDMKYAAKALGGQVINKPEDLAPETIGTAGKLEMLEDLEMTK
HHHHHCCCCEEEEEECCCHHHHHHHHHHCCHHCCCCHHCCCHHCCCCCHHHHHHHHHHHH
LSECKNPKAVTILLRGSSQHLVDELERAIEDAKRVVQDVVEDGSYTIGGGSVETELSLRL
HHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHH
REYAATEGGRVQLAIEGYAKAFEIIPKTLAENSGFDTVDKVIDLRQAHAMGQKYAGLDVF
HHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
TGTVVDMKELGVVEPKRVKRQAIQSASETAMLLIRVDDMMISKGAGQM
CCCEECHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCC
>Mature Secondary Structure 
ANNSGQQVVILRNNVERVPGQEALRSNIMAAKVLGNTVRTTLGPRGMDKMMVTQGSDDI
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCE
VITNDGATILHQIHVEHPGAKLVVEVAETQDNECGDGTTTAVVMVGSFMEQAEHLIDSGV
EEECCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
HPSVIAKGYNLGMLKALELLEKLAIDVTPKDKNMLKLIAKTAMTGKSIESIMDQACDVVV
CHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
EAVSGVAETSGKKTQVNEDDILVKTKRSDTMSAELIKGVLIDKTRLDSLMPKKIKGVKAA
HHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHHCHHHHCCCHHH
FIAGPLEITKTQTKSKIKITSAEQLSAFSSAERATLKAMADKFAANGVNVVLCQKGIADP
EEECCCCEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
VQYYLGEHGIYALEFVPEKDMKYAAKALGGQVINKPEDLAPETIGTAGKLEMLEDLEMTK
HHHHHCCCCEEEEEECCCHHHHHHHHHHCCHHCCCCHHCCCHHCCCCCHHHHHHHHHHHH
LSECKNPKAVTILLRGSSQHLVDELERAIEDAKRVVQDVVEDGSYTIGGGSVETELSLRL
HHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHH
REYAATEGGRVQLAIEGYAKAFEIIPKTLAENSGFDTVDKVIDLRQAHAMGQKYAGLDVF
HHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
TGTVVDMKELGVVEPKRVKRQAIQSASETAMLLIRVDDMMISKGAGQM
CCCEECHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]