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The map label for this gene is hbpA [H]

Identifier: 124485545

GI number: 124485545

Start: 694933

End: 696501

Strand: Reverse

Name: hbpA [H]

Synonym: Mlab_0722

Alternate gene names: 124485545

Gene position: 696501-694933 (Counterclockwise)

Preceding gene: 124485546

Following gene: 124485544

Centisome position: 38.59

GC content: 54.11

Gene sequence:

>1569_bases
ATGCAGAGTGGTTTCCAGAAAATAATCTTGCTCATAAGTATCGCGGTCCTCCTTATCGCCGCGGTTTGCGTGGCAGGATG
CGTTCAGTCATCCGATTCCGGAGAAAAAGTTCTTCGTCTCGTGGAGATCGAGGGCCCGGACACGGGCGGCAGTCTCGACC
CGGCAAACGGCTGGGAAGGATGGTATGTCGATAAAGCAGGCATTTACGAGACCCTGTTCGCATACGACCCGGACATGGTC
CTCCAGCCCAAACTTGCGACCGGATACAAGCTTTTGAACGACACGACGTGGGAGATCACTCTGCGTAAAGGTGTTACGTT
CCATGACGGGACGCCGTTCAATGCCGACGCGGTCATTTTCTCGTTCAACCGTGTCCTCAATGCATCAAACAGCCGTGCTT
ACGAGTATGCGTTCATTGAAGATGTCAGAAAAACGGACGACTATACGATCATCATCGAGACCAATAAACCGTATGCTCCG
CTGATCGCATCCCTCGTCGACCCGATCATGTCTATCGTCAGTCCGAACATCGTCGATGTCAACAAACAACCGGTCGGAAC
GGGACCGTTCGTTTTCTCCGCGCTTGAATCCGGCGCAAGCCTGGACGTCGTGAGAAACGAGAATTACTGGGGCGGCAAAG
TAGGACTTGCCGGAGTGAACACGACCTACATCGGTGATGCCACCGCACGTACGCTTCTCATCAAATCTGGTGATGCCGAC
GTAGTTCGTGACATTCTCCCAAGCGAGTATGCAGCCGTGAAAAACGCGGCAGACACGCATGTCGAATCGAAAGCGATGCT
CCGTACGTACTTTGTCTACATGAACGAAAACAAAGCGCCGTTCAATGACGTCCGCGTCCGTCAGGCACTCAGTTACGCAG
TGAACCGTCAGGAGATCGTCGACACGGCGCTTGAAGGCGTCGGCGGCGTTGTCGCGGTCGGTCCGTTCTCATACTCCTCG
CCGTGGAACGCAAACGACGAGATCGAATCATATGCATACAACAAAGAAAAGGCGCTGGCTCTCTTAGCCGAGGCAGGGAT
CCTGCCGGGGGCTGACGGGAAACTGTATTACAACGGCAAACCGTTCACCATCGAGATAACGACCTACTCCAAACGTGCGG
CTCTCCCGCCGACACTGGAAGTCATCGCAGCCCAGTATGAAGATCTGGGTATTACCGTCAACACGCGTATCATGGAAAGC
AGCGCCATCAAAGTCGATGTCGCCGCCGGAAATTACGACATGACGATGGCCGCCTGGTCGACCATGCCGACCGGAGACCC
GGATTATTTCCTGAGCAGAATGTTCTTCTCGACCGCCGCGTATGCTTCCACCTGGCTGCACTACTCCAACCCCGAAGTCG
ATGAACTCATCCTGAAAGCAAGCACGACCTTCGATCAGGCGGAGCGGGCTGAACTGTACGACGAGATCCAGAACATCACC
CAGAACGACGCAGGACTGATCTATCTGTTCTATGAATCGCAGAACTGGGGAGTCGGCAATGATGTCCTGAATCTCGAGAT
CTACCCGAACGAATACACGATGATGACCAAAGACATCACCATCACCTGA

Upstream 100 bases:

>100_bases
GAATATAATATTTGTAGATTTTAAAAACATACGAATAAAAAGAGGCAATTAATATAATATTACAAACATAAAATTTGTTC
ATACTTTTCGAGGTAAAAAC

Downstream 100 bases:

>100_bases
ATCCCATGTCGAACGAAACGATCCGCAAACACTGGAACGAGCTGAGTCCGGGATATCGGAAACGATATCATGCATATCTC
GACGAGGAGATCGTTTTGAT

Product: radical SAM domain-containing protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Hemin-binding lipoprotein [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MQSGFQKIILLISIAVLLIAAVCVAGCVQSSDSGEKVLRLVEIEGPDTGGSLDPANGWEGWYVDKAGIYETLFAYDPDMV
LQPKLATGYKLLNDTTWEITLRKGVTFHDGTPFNADAVIFSFNRVLNASNSRAYEYAFIEDVRKTDDYTIIIETNKPYAP
LIASLVDPIMSIVSPNIVDVNKQPVGTGPFVFSALESGASLDVVRNENYWGGKVGLAGVNTTYIGDATARTLLIKSGDAD
VVRDILPSEYAAVKNAADTHVESKAMLRTYFVYMNENKAPFNDVRVRQALSYAVNRQEIVDTALEGVGGVVAVGPFSYSS
PWNANDEIESYAYNKEKALALLAEAGILPGADGKLYYNGKPFTIEITTYSKRAALPPTLEVIAAQYEDLGITVNTRIMES
SAIKVDVAAGNYDMTMAAWSTMPTGDPDYFLSRMFFSTAAYASTWLHYSNPEVDELILKASTTFDQAERAELYDEIQNIT
QNDAGLIYLFYESQNWGVGNDVLNLEIYPNEYTMMTKDITIT

Sequences:

>Translated_522_residues
MQSGFQKIILLISIAVLLIAAVCVAGCVQSSDSGEKVLRLVEIEGPDTGGSLDPANGWEGWYVDKAGIYETLFAYDPDMV
LQPKLATGYKLLNDTTWEITLRKGVTFHDGTPFNADAVIFSFNRVLNASNSRAYEYAFIEDVRKTDDYTIIIETNKPYAP
LIASLVDPIMSIVSPNIVDVNKQPVGTGPFVFSALESGASLDVVRNENYWGGKVGLAGVNTTYIGDATARTLLIKSGDAD
VVRDILPSEYAAVKNAADTHVESKAMLRTYFVYMNENKAPFNDVRVRQALSYAVNRQEIVDTALEGVGGVVAVGPFSYSS
PWNANDEIESYAYNKEKALALLAEAGILPGADGKLYYNGKPFTIEITTYSKRAALPPTLEVIAAQYEDLGITVNTRIMES
SAIKVDVAAGNYDMTMAAWSTMPTGDPDYFLSRMFFSTAAYASTWLHYSNPEVDELILKASTTFDQAERAELYDEIQNIT
QNDAGLIYLFYESQNWGVGNDVLNLEIYPNEYTMMTKDITIT
>Mature_522_residues
MQSGFQKIILLISIAVLLIAAVCVAGCVQSSDSGEKVLRLVEIEGPDTGGSLDPANGWEGWYVDKAGIYETLFAYDPDMV
LQPKLATGYKLLNDTTWEITLRKGVTFHDGTPFNADAVIFSFNRVLNASNSRAYEYAFIEDVRKTDDYTIIIETNKPYAP
LIASLVDPIMSIVSPNIVDVNKQPVGTGPFVFSALESGASLDVVRNENYWGGKVGLAGVNTTYIGDATARTLLIKSGDAD
VVRDILPSEYAAVKNAADTHVESKAMLRTYFVYMNENKAPFNDVRVRQALSYAVNRQEIVDTALEGVGGVVAVGPFSYSS
PWNANDEIESYAYNKEKALALLAEAGILPGADGKLYYNGKPFTIEITTYSKRAALPPTLEVIAAQYEDLGITVNTRIMES
SAIKVDVAAGNYDMTMAAWSTMPTGDPDYFLSRMFFSTAAYASTWLHYSNPEVDELILKASTTFDQAERAELYDEIQNIT
QNDAGLIYLFYESQNWGVGNDVLNLEIYPNEYTMMTKDITIT

Specific function: Important role in heme acquisition or metabolism [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=435, Percent_Identity=28.9655172413793, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1787762, Length=484, Percent_Identity=27.2727272727273, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI1789887, Length=460, Percent_Identity=30.4347826086957, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1787052, Length=448, Percent_Identity=27.6785714285714, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787551, Length=478, Percent_Identity=24.4769874476987, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787495, Length=519, Percent_Identity=24.4701348747592, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI87081878, Length=512, Percent_Identity=23.6328125, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57357; Mature: 57357

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSGFQKIILLISIAVLLIAAVCVAGCVQSSDSGEKVLRLVEIEGPDTGGSLDPANGWEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCC
WYVDKAGIYETLFAYDPDMVLQPKLATGYKLLNDTTWEITLRKGVTFHDGTPFNADAVIF
EEEECCCHHHHHHHCCCCCEECCCHHCCEEEECCCEEEEEEECCEEECCCCCCCCCEEEE
SFNRVLNASNSRAYEYAFIEDVRKTDDYTIIIETNKPYAPLIASLVDPIMSIVSPNIVDV
EEHHHHCCCCCCEEEEHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEC
NKQPVGTGPFVFSALESGASLDVVRNENYWGGKVGLAGVNTTYIGDATARTLLIKSGDAD
CCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCEEEECCCCEEEEEEECCCHH
VVRDILPSEYAAVKNAADTHVESKAMLRTYFVYMNENKAPFNDVRVRQALSYAVNRQEIV
HHHHHCCHHHHHHHCCHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHH
DTALEGVGGVVAVGPFSYSSPWNANDEIESYAYNKEKALALLAEAGILPGADGKLYYNGK
HHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCEEEECCC
PFTIEITTYSKRAALPPTLEVIAAQYEDLGITVNTRIMESSAIKVDVAAGNYDMTMAAWS
EEEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEEEECCCEEEEEECCCCEEEEEEEC
TMPTGDPDYFLSRMFFSTAAYASTWLHYSNPEVDELILKASTTFDQAERAELYDEIQNIT
CCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHEEECCCCCCHHHHHHHHHHHHHCC
QNDAGLIYLFYESQNWGVGNDVLNLEIYPNEYTMMTKDITIT
CCCCCEEEEEEECCCCCCCCCEEEEEEECCCEEEEEEEEEEC
>Mature Secondary Structure
MQSGFQKIILLISIAVLLIAAVCVAGCVQSSDSGEKVLRLVEIEGPDTGGSLDPANGWEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCC
WYVDKAGIYETLFAYDPDMVLQPKLATGYKLLNDTTWEITLRKGVTFHDGTPFNADAVIF
EEEECCCHHHHHHHCCCCCEECCCHHCCEEEECCCEEEEEEECCEEECCCCCCCCCEEEE
SFNRVLNASNSRAYEYAFIEDVRKTDDYTIIIETNKPYAPLIASLVDPIMSIVSPNIVDV
EEHHHHCCCCCCEEEEHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEC
NKQPVGTGPFVFSALESGASLDVVRNENYWGGKVGLAGVNTTYIGDATARTLLIKSGDAD
CCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCEEEECCCCEEEEEEECCCHH
VVRDILPSEYAAVKNAADTHVESKAMLRTYFVYMNENKAPFNDVRVRQALSYAVNRQEIV
HHHHHCCHHHHHHHCCHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHH
DTALEGVGGVVAVGPFSYSSPWNANDEIESYAYNKEKALALLAEAGILPGADGKLYYNGK
HHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCEEEECCC
PFTIEITTYSKRAALPPTLEVIAAQYEDLGITVNTRIMESSAIKVDVAAGNYDMTMAAWS
EEEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEEEECCCEEEEEECCCCEEEEEEEC
TMPTGDPDYFLSRMFFSTAAYASTWLHYSNPEVDELILKASTTFDQAERAELYDEIQNIT
CCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHEEECCCCCCHHHHHHHHHHHHHCC
QNDAGLIYLFYESQNWGVGNDVLNLEIYPNEYTMMTKDITIT
CCCCCEEEEEEECCCCCCCCCEEEEEEECCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1339409; 7542800; 2041470 [H]