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The map label for this gene is purC [H]

Identifier: 124485499

GI number: 124485499

Start: 647306

End: 647986

Strand: Reverse

Name: purC [H]

Synonym: Mlab_0676

Alternate gene names: 124485499

Gene position: 647986-647306 (Counterclockwise)

Preceding gene: 124485500

Following gene: 124485498

Centisome position: 35.9

GC content: 47.28

Gene sequence:

>681_bases
ATGAAATTGGTATACACCGGCAAAACAAAGGATGTATTCTCACTAGAAGATGGGAACTATCTGCTGAAATTTAAGGATAA
CTGTACTGGTGCTGATGGGGTATTCGATCCAGGGATGAACACAGTTGGCCCAACTATTGAAGGTGCCGGACGTGCTGGTC
TTAGATTAACTGAGTACTTTATGAAGATCCTGAACGAAAAAGGGGTCCCAACTCACTTCATCAGTGCAGACATCGAAAAT
GTAACGATGACCGTAAAGCCCGCCAAGACATTCGGCAAGGGACTTGAAGTCATCTGCAGATTCCGTGCAGTCGGTAGCTT
CTACCGTCGCTATGGCGACTACATTACGGAAGCAGCAGTACTGCCGGCATTTGTCGAGACAACGTTCAAAGATGATGCAC
GTGAAGACCCGCCAGTAACCAAAGATGCACTTGAAATTCTGGGCATCATGTCCCCCGAGGATTATGAGAGCCTGAAAGTT
CTCACCCAGAAAATCGCCGGCATCATGAAGGAAGAGCTCGCAAAGAAGAACATTGAACTCTACGACATTAAGTTCGAGTT
CGGTAAGATCGGCGACCAGGTTGTTTTAATCGATGAGATCTCCGGCGGAAACATGCGTGCATACAGAAACGGCGAGTACC
TTTTACCGCTCGATCTGACAAGCAAGGTTCTCGACGAGTAA

Upstream 100 bases:

>100_bases
CTGAACGGCGAACCCTACCACAAATAACAGCGTATGGCCATGTTACCTGTTGTCATGTAGCATCAATATTATACGTGGTT
AGTTCCAACATATAAGTACC

Downstream 100 bases:

>100_bases
CCATCCCTTTTTTTCTAAAAAAATACTTATATTAGTCTGGTCCATCATTCAGTATGAAGAAAATCAGACTGATGATTCTT
GGAGCATGCATACTTGTCAT

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKLVYTGKTKDVFSLEDGNYLLKFKDNCTGADGVFDPGMNTVGPTIEGAGRAGLRLTEYFMKILNEKGVPTHFISADIEN
VTMTVKPAKTFGKGLEVICRFRAVGSFYRRYGDYITEAAVLPAFVETTFKDDAREDPPVTKDALEILGIMSPEDYESLKV
LTQKIAGIMKEELAKKNIELYDIKFEFGKIGDQVVLIDEISGGNMRAYRNGEYLLPLDLTSKVLDE

Sequences:

>Translated_226_residues
MKLVYTGKTKDVFSLEDGNYLLKFKDNCTGADGVFDPGMNTVGPTIEGAGRAGLRLTEYFMKILNEKGVPTHFISADIEN
VTMTVKPAKTFGKGLEVICRFRAVGSFYRRYGDYITEAAVLPAFVETTFKDDAREDPPVTKDALEILGIMSPEDYESLKV
LTQKIAGIMKEELAKKNIELYDIKFEFGKIGDQVVLIDEISGGNMRAYRNGEYLLPLDLTSKVLDE
>Mature_226_residues
MKLVYTGKTKDVFSLEDGNYLLKFKDNCTGADGVFDPGMNTVGPTIEGAGRAGLRLTEYFMKILNEKGVPTHFISADIEN
VTMTVKPAKTFGKGLEVICRFRAVGSFYRRYGDYITEAAVLPAFVETTFKDDAREDPPVTKDALEILGIMSPEDYESLKV
LTQKIAGIMKEELAKKNIELYDIKFEFGKIGDQVVLIDEISGGNMRAYRNGEYLLPLDLTSKVLDE

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=221, Percent_Identity=28.0542986425339, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI5453539, Length=221, Percent_Identity=28.0542986425339, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1788820, Length=212, Percent_Identity=30.188679245283, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 25180; Mature: 25180

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVYTGKTKDVFSLEDGNYLLKFKDNCTGADGVFDPGMNTVGPTIEGAGRAGLRLTEYF
CEEEEECCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
MKILNEKGVPTHFISADIENVTMTVKPAKTFGKGLEVICRFRAVGSFYRRYGDYITEAAV
HHHHCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPAFVETTFKDDAREDPPVTKDALEILGIMSPEDYESLKVLTQKIAGIMKEELAKKNIEL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
YDIKFEFGKIGDQVVLIDEISGGNMRAYRNGEYLLPLDLTSKVLDE
EEEEEECCCCCCEEEEEEECCCCCEEEEECCCEEEECCCHHHHHCC
>Mature Secondary Structure
MKLVYTGKTKDVFSLEDGNYLLKFKDNCTGADGVFDPGMNTVGPTIEGAGRAGLRLTEYF
CEEEEECCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
MKILNEKGVPTHFISADIENVTMTVKPAKTFGKGLEVICRFRAVGSFYRRYGDYITEAAV
HHHHCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPAFVETTFKDDAREDPPVTKDALEILGIMSPEDYESLKVLTQKIAGIMKEELAKKNIEL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
YDIKFEFGKIGDQVVLIDEISGGNMRAYRNGEYLLPLDLTSKVLDE
EEEEEECCCCCCEEEEEEECCCCCEEEEECCCEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]