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The map label for this gene is rpoE1 [H]

Identifier: 124485482

GI number: 124485482

Start: 627830

End: 628402

Strand: Reverse

Name: rpoE1 [H]

Synonym: Mlab_0658

Alternate gene names: 124485482

Gene position: 628402-627830 (Counterclockwise)

Preceding gene: 124485483

Following gene: 124485481

Centisome position: 34.82

GC content: 51.83

Gene sequence:

>573_bases
ATGTATTATAAACTGAAACTCAACGACAAAGTCAGAGTCCCCCCGGAACGCATGGGTGAAGATCTTAACACGGTTATCCT
AGACGTCCTGCAGGAACAGTTTGAAGGAAGTGTCGACAAAGAGATGGGTATTTTCATCGCAGTGACCGGCGTAAACCGCG
TCGGTGATGGAGAGATCATCTACAGTGACGGAGGGGCATACTATGATGTCGATTTCGAAGCTGTCGTCCTCCGCTTATCC
CTTCAGGAGATTATCGAGGGTGTTGTTGTCGAGACGACAAGCTTTGGCGCATTCGTGTCTCTTGGGCCGATCGATGCCAT
GCTGCACATGAGTCAGATCTCCGACGAGTACATCGATTATGACGAGAAGAACTCGCGTCTGGTCTGCAAAGACAGCGGCA
GAACGATCGGTGTCGGCGACACTCTGCGTGCACGTGTCGTTGCTCTTTCCTTAAACGAACGCGATCCCCGCGAATCGAAG
ATCGGTCTCACCATGCGCCAGCCAGGTCTTGGAACCCTGGCCTGGATCGAAGAGGACATGAACAAAGAGAAAGCCGAGAA
GGAGCTCAAGTAA

Upstream 100 bases:

>100_bases
AAATCGTCTGAGACAACGCCGTATTCCAGTTGTTGTTCTCAGATCGCGCTGCAAACTAGAATTAATCGGAAAATAAGCAA
AACAGGCGAGGAGACAAAAT

Downstream 100 bases:

>100_bases
TGGCAGCAAAACGTTCCACGCAAAAGAAAGTCCTGCAGGCTTGCCGAAACTGTCACAAAATTCTTGAGGCGGATAAAACC
GTCTGCCCCGAATGTCAGGG

Product: DNA-directed RNA polymerase subunit E'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MYYKLKLNDKVRVPPERMGEDLNTVILDVLQEQFEGSVDKEMGIFIAVTGVNRVGDGEIIYSDGGAYYDVDFEAVVLRLS
LQEIIEGVVVETTSFGAFVSLGPIDAMLHMSQISDEYIDYDEKNSRLVCKDSGRTIGVGDTLRARVVALSLNERDPRESK
IGLTMRQPGLGTLAWIEEDMNKEKAEKELK

Sequences:

>Translated_190_residues
MYYKLKLNDKVRVPPERMGEDLNTVILDVLQEQFEGSVDKEMGIFIAVTGVNRVGDGEIIYSDGGAYYDVDFEAVVLRLS
LQEIIEGVVVETTSFGAFVSLGPIDAMLHMSQISDEYIDYDEKNSRLVCKDSGRTIGVGDTLRARVVALSLNERDPRESK
IGLTMRQPGLGTLAWIEEDMNKEKAEKELK
>Mature_190_residues
MYYKLKLNDKVRVPPERMGEDLNTVILDVLQEQFEGSVDKEMGIFIAVTGVNRVGDGEIIYSDGGAYYDVDFEAVVLRLS
LQEIIEGVVVETTSFGAFVSLGPIDAMLHMSQISDEYIDYDEKNSRLVCKDSGRTIGVGDTLRARVVALSLNERDPRESK
IGLTMRQPGLGTLAWIEEDMNKEKAEKELK

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG1095

COG function: function code K; DNA-directed RNA polymerase, subunit E'

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI4505947, Length=158, Percent_Identity=26.5822784810127, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510349, Length=158, Percent_Identity=27.2151898734177, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR005576
- InterPro:   IPR004519 [H]

Pfam domain/function: PF03876 RNA_pol_Rpb7_N; PF00575 S1 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 21242; Mature: 21242

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYKLKLNDKVRVPPERMGEDLNTVILDVLQEQFEGSVDKEMGIFIAVTGVNRVGDGEII
CEEEEEECCCEECCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEE
YSDGGAYYDVDFEAVVLRLSLQEIIEGVVVETTSFGAFVSLGPIDAMLHMSQISDEYIDY
ECCCCEEEECCHHHHHHHHHHHHHHCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHCCC
DEKNSRLVCKDSGRTIGVGDTLRARVVALSLNERDPRESKIGLTMRQPGLGTLAWIEEDM
CCCCCEEEEECCCCEECCCCCCEEEEEEEECCCCCCCCHHCCEEEECCCCCCHHHHHHHH
NKEKAEKELK
CHHHHHHHCC
>Mature Secondary Structure
MYYKLKLNDKVRVPPERMGEDLNTVILDVLQEQFEGSVDKEMGIFIAVTGVNRVGDGEII
CEEEEEECCCEECCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEE
YSDGGAYYDVDFEAVVLRLSLQEIIEGVVVETTSFGAFVSLGPIDAMLHMSQISDEYIDY
ECCCCEEEECCHHHHHHHHHHHHHHCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHCCC
DEKNSRLVCKDSGRTIGVGDTLRARVVALSLNERDPRESKIGLTMRQPGLGTLAWIEEDM
CCCCCEEEEECCCCEECCCCCCEEEEEEEECCCCCCCCHHCCEEEECCCCCCHHHHHHHH
NKEKAEKELK
CHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]