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Identifier: 124485445

GI number: 124485445

Start: 595504

End: 597144

Strand: Reverse

Name: lig

Synonym: Mlab_0620

Alternate gene names: 124485445

Gene position: 597144-595504 (Counterclockwise)

Preceding gene: 124485446

Following gene: 124485443

Centisome position: 33.08

GC content: 54.05

Gene sequence:

>1641_bases
ATGCAGTTTGTCGAGTTTGCAGAGATATGTAATACGATCGAGGGAACATCCTCGCGTCTTGCGACGGCCGATATTCTGGC
CGAAAAATTTCCTCTACTCACCGAAGAGGAACTGCCCGTCTTCGTCAGATTCATGCGGGGAAGACTCTTTCCCGACTGGT
CGTCCGAGAAGCTCGGGTTCGGCCCGAATCTTTTGTATGATGCCCTTGCCTACGTCATCGGTAAAAAGCGGAGTTATGTG
GTCTCGGCGATCAACAACGCAGGTGATATCGGTAAAGTCGTCGAGAGTCTTATCGAAAAACGGGAGCAGACGATGTTTTT
CTCGGAAGAACTGGATCTTCTGGATGTGAACGCCCGGTTTCTGCAGATGGCAAAATCCTCGGGAAGACGATCTCAGCAGG
AGCGGCTGAGATCCGCCCAGTATCTTCTTTCGAACGCAACGCCGCTTGAGGGGAGATACCTTGCCCGTCTCATGCTCGAA
GAGATGCGGATCGGTGTTGGAGAAGGCGTCGTAAAAGACGCGGTCTCAAAAGCGTTCGGCGTTCCGTCAGACATTGTCGA
ACACGCTCATCAGGCTCTCAACGACCTCGGAGAGGTGGCGTTCCTCGCAAAAACCGACCCCTCCCGGCTTTCCAATGTTC
ATATCACCGCGTTTCGCCCGGTAAAAATGATGCTTGCCCAGCAGGGCTCCATCACCTCGATGGTCGAAACGCATGGAACA
CTCGCTGCGGAAAACAAATACGACGGCAGCAGATTCCAGTTCCATAAATCCGGCGGGAAGTGTGCGATCTACTCCAGGCG
TCTTGAAGAGATGACGGCGTCACTTCCCGATGTTGTTAAGATGCTGGATAAGGCGACCGATCATGACGTCATCATCGACG
GCGAGGTGATCGCGATCATGAATGGAAAACCCATGCCTTTCCAGACAATCCTTCGGCGGATCCGCAGGAAGCATGATGTC
GGGGATGCGCAGGAGGCAATCACCCTTCTTCCCTGGGTGTTCGATATCCTTGCTGCCGACGGAGAGACCCTGATCGATCT
GCCCTTTAGGGAGCGGCGCAAGATCCTTGAATCCGTCATGAATGCGTATGTCGCACCGCAGCTCGTCAGCGACTCCGCAG
AAGAGATCGAAGCGTATTACCACTCCTCGCTCGACAATGGGAATGAAGGGATCATGCTCAAAGTGCTCGACTCGCCGTAT
CTTCCGGGAAATCGTGGCAAGCTCTGGATCAAGATCAAGCCCGAGGTCGATACGATCGACCTGGTGGTGACGGCGGCCGA
GTGGGGCGAAGGAAAACGTGCGAAGATGTTCGGCTCGTTCCTTCTTGCCTGTCAGGATGAGAACGGCGACCTTCTGGAGA
TCTCGCGGGTGGCGACCGGTATCGACGACTCTATGCTTTCGACCCTGTATGATCTTTTCAAAGACAAGATCATTGCCGAA
AAGGGAAAGACCGTGACCTTCGAACCGGATGTGGTCTTTGAGGTGGGGTATGCGGAACTGCAGAAAAGTACGAATTACGA
GGCCGGGTATGCTCTCCGGTTCCCGCGTTTTGTCAGGCTTAGGGACGACAAGGATGTCTCGGAGATCGAGACGCTGGAAA
GCCTGACCCGCCGGTATTCTCTGCAGAATAAGGAAGAGTAA

Upstream 100 bases:

>100_bases
CTTTGGCTGCGGCGGGCGAGAGCGGAACGGAAATTTACGTCAGTGATCAGCTTGCGGATTACCGGGAACGCCGCCTTGTC
CGGTTCGAGGTGTAAAGAAC

Downstream 100 bases:

>100_bases
ACGCGAACAAAAAAACAGGCCGATACTCCTGGGCGGACTCGAACCACCGTCTCCGGCTCCAAAGGCCGGAATGATTGACC
ACTACACTACAGGAGTTGGC

Product: hypothetical protein

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MQFVEFAEICNTIEGTSSRLATADILAEKFPLLTEEELPVFVRFMRGRLFPDWSSEKLGFGPNLLYDALAYVIGKKRSYV
VSAINNAGDIGKVVESLIEKREQTMFFSEELDLLDVNARFLQMAKSSGRRSQQERLRSAQYLLSNATPLEGRYLARLMLE
EMRIGVGEGVVKDAVSKAFGVPSDIVEHAHQALNDLGEVAFLAKTDPSRLSNVHITAFRPVKMMLAQQGSITSMVETHGT
LAAENKYDGSRFQFHKSGGKCAIYSRRLEEMTASLPDVVKMLDKATDHDVIIDGEVIAIMNGKPMPFQTILRRIRRKHDV
GDAQEAITLLPWVFDILAADGETLIDLPFRERRKILESVMNAYVAPQLVSDSAEEIEAYYHSSLDNGNEGIMLKVLDSPY
LPGNRGKLWIKIKPEVDTIDLVVTAAEWGEGKRAKMFGSFLLACQDENGDLLEISRVATGIDDSMLSTLYDLFKDKIIAE
KGKTVTFEPDVVFEVGYAELQKSTNYEAGYALRFPRFVRLRDDKDVSEIETLESLTRRYSLQNKEE

Sequences:

>Translated_546_residues
MQFVEFAEICNTIEGTSSRLATADILAEKFPLLTEEELPVFVRFMRGRLFPDWSSEKLGFGPNLLYDALAYVIGKKRSYV
VSAINNAGDIGKVVESLIEKREQTMFFSEELDLLDVNARFLQMAKSSGRRSQQERLRSAQYLLSNATPLEGRYLARLMLE
EMRIGVGEGVVKDAVSKAFGVPSDIVEHAHQALNDLGEVAFLAKTDPSRLSNVHITAFRPVKMMLAQQGSITSMVETHGT
LAAENKYDGSRFQFHKSGGKCAIYSRRLEEMTASLPDVVKMLDKATDHDVIIDGEVIAIMNGKPMPFQTILRRIRRKHDV
GDAQEAITLLPWVFDILAADGETLIDLPFRERRKILESVMNAYVAPQLVSDSAEEIEAYYHSSLDNGNEGIMLKVLDSPY
LPGNRGKLWIKIKPEVDTIDLVVTAAEWGEGKRAKMFGSFLLACQDENGDLLEISRVATGIDDSMLSTLYDLFKDKIIAE
KGKTVTFEPDVVFEVGYAELQKSTNYEAGYALRFPRFVRLRDDKDVSEIETLESLTRRYSLQNKEE
>Mature_546_residues
MQFVEFAEICNTIEGTSSRLATADILAEKFPLLTEEELPVFVRFMRGRLFPDWSSEKLGFGPNLLYDALAYVIGKKRSYV
VSAINNAGDIGKVVESLIEKREQTMFFSEELDLLDVNARFLQMAKSSGRRSQQERLRSAQYLLSNATPLEGRYLARLMLE
EMRIGVGEGVVKDAVSKAFGVPSDIVEHAHQALNDLGEVAFLAKTDPSRLSNVHITAFRPVKMMLAQQGSITSMVETHGT
LAAENKYDGSRFQFHKSGGKCAIYSRRLEEMTASLPDVVKMLDKATDHDVIIDGEVIAIMNGKPMPFQTILRRIRRKHDV
GDAQEAITLLPWVFDILAADGETLIDLPFRERRKILESVMNAYVAPQLVSDSAEEIEAYYHSSLDNGNEGIMLKVLDSPY
LPGNRGKLWIKIKPEVDTIDLVVTAAEWGEGKRAKMFGSFLLACQDENGDLLEISRVATGIDDSMLSTLYDLFKDKIIAE
KGKTVTFEPDVVFEVGYAELQKSTNYEAGYALRFPRFVRLRDDKDVSEIETLESLTRRYSLQNKEE

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=625, Percent_Identity=27.68, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI73747829, Length=506, Percent_Identity=27.4703557312253, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI73747844, Length=506, Percent_Identity=27.4703557312253, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI46255052, Length=503, Percent_Identity=26.6401590457256, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI23199993, Length=503, Percent_Identity=26.6401590457256, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI148539894, Length=503, Percent_Identity=26.6401590457256, Blast_Score=139, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71985269, Length=605, Percent_Identity=28.5950413223141, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320038, Length=610, Percent_Identity=29.8360655737705, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6324578, Length=236, Percent_Identity=27.5423728813559, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24762424, Length=619, Percent_Identity=27.6252019386107, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI85815841, Length=453, Percent_Identity=29.5805739514349, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24641829, Length=514, Percent_Identity=24.124513618677, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI_METLZ (A2SR38)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030061.1
- ProteinModelPortal:   A2SR38
- SMR:   A2SR38
- STRING:   A2SR38
- GeneID:   4795702
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0620
- eggNOG:   arNOG04596
- HOGENOM:   HBG570821
- OMA:   GRPRPFQ
- PhylomeDB:   A2SR38
- ProtClustDB:   CLSK949919
- BioCyc:   MLAB410358:MLAB_0620-MONOMER
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 61359; Mature: 61359

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 246-246 BINDING 244-244 BINDING 251-251 BINDING 266-266 BINDING 295-295 BINDING 334-334 BINDING 405-405 BINDING 411-411

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFVEFAEICNTIEGTSSRLATADILAEKFPLLTEEELPVFVRFMRGRLFPDWSSEKLGF
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
GPNLLYDALAYVIGKKRSYVVSAINNAGDIGKVVESLIEKREQTMFFSEELDLLDVNARF
CHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHH
LQMAKSSGRRSQQERLRSAQYLLSNATPLEGRYLARLMLEEMRIGVGEGVVKDAVSKAFG
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
VPSDIVEHAHQALNDLGEVAFLAKTDPSRLSNVHITAFRPVKMMLAQQGSITSMVETHGT
CCHHHHHHHHHHHHHHHHEEEEECCCHHHHCCEEEEEECHHHHHHHCCCCHHHHHHHCCC
LAAENKYDGSRFQFHKSGGKCAIYSRRLEEMTASLPDVVKMLDKATDHDVIIDGEVIAIM
EEECCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEE
NGKPMPFQTILRRIRRKHDVGDAQEAITLLPWVFDILAADGETLIDLPFRERRKILESVM
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHH
NAYVAPQLVSDSAEEIEAYYHSSLDNGNEGIMLKVLDSPYLPGNRGKLWIKIKPEVDTID
HHHHCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEE
LVVTAAEWGEGKRAKMFGSFLLACQDENGDLLEISRVATGIDDSMLSTLYDLFKDKIIAE
EEEEECCCCCCCHHHHHHHEEEEEECCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHC
KGKTVTFEPDVVFEVGYAELQKSTNYEAGYALRFPRFVRLRDDKDVSEIETLESLTRRYS
CCCEEEECCCEEEECCHHHHHHCCCCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHHC
LQNKEE
CCCCCC
>Mature Secondary Structure
MQFVEFAEICNTIEGTSSRLATADILAEKFPLLTEEELPVFVRFMRGRLFPDWSSEKLGF
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
GPNLLYDALAYVIGKKRSYVVSAINNAGDIGKVVESLIEKREQTMFFSEELDLLDVNARF
CHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHH
LQMAKSSGRRSQQERLRSAQYLLSNATPLEGRYLARLMLEEMRIGVGEGVVKDAVSKAFG
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
VPSDIVEHAHQALNDLGEVAFLAKTDPSRLSNVHITAFRPVKMMLAQQGSITSMVETHGT
CCHHHHHHHHHHHHHHHHEEEEECCCHHHHCCEEEEEECHHHHHHHCCCCHHHHHHHCCC
LAAENKYDGSRFQFHKSGGKCAIYSRRLEEMTASLPDVVKMLDKATDHDVIIDGEVIAIM
EEECCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEE
NGKPMPFQTILRRIRRKHDVGDAQEAITLLPWVFDILAADGETLIDLPFRERRKILESVM
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHH
NAYVAPQLVSDSAEEIEAYYHSSLDNGNEGIMLKVLDSPYLPGNRGKLWIKIKPEVDTID
HHHHCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEE
LVVTAAEWGEGKRAKMFGSFLLACQDENGDLLEISRVATGIDDSMLSTLYDLFKDKIIAE
EEEEECCCCCCCHHHHHHHEEEEEECCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHC
KGKTVTFEPDVVFEVGYAELQKSTNYEAGYALRFPRFVRLRDDKDVSEIETLESLTRRYS
CCCEEEECCCEEEECCHHHHHHCCCCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHHC
LQNKEE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA