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The map label for this gene is pheA [H]

Identifier: 124485423

GI number: 124485423

Start: 567641

End: 568438

Strand: Reverse

Name: pheA [H]

Synonym: Mlab_0598

Alternate gene names: 124485423

Gene position: 568438-567641 (Counterclockwise)

Preceding gene: 124485424

Following gene: 124485422

Centisome position: 31.49

GC content: 51.13

Gene sequence:

>798_bases
ATGACATTGGCAGTACTCGGCCCGAAAGGCACGTTCTCCTGCGAGCTTGCCGAAAAAATCAGGAACGAAAATGAAGAGAT
CATTCTGTTCCCAACGATCCGGGACGTTTTCACCGCGGTCCTCGAGAAAAATATCCGGGGCATCGTTCCGGTTGAAAACA
GCGAGGCGGGCGGCGTTGGCGAAACCCTGGACGGCCTTTTACAGACTGAGTGCCGTATCACCGCTGAATATTATATGCCG
ATCCGTCATTTCTTCGTTTCAAGATACAGTCCTGATGAGATCTCGGTCATCTACACTCATCCGCAGTCCCATGAGCAGTG
CAGCATCTATCTGAACGGCATGAAAAGGGCCTCCCTGATCCACACGAGCAGCAATGCCCAGAGTGCCAAAGAGGCGTCGT
TTATCAGCGGATCGGCGGCCGTAACGACGGAAAGCGCCGCAAAACTGTATGATCTGCCCATACTGCAGAAGGATATTCAG
AATTCACTAAACAATACCACGCGGTTTCTTGAGATCTCGGCAGGTGCGCTAGATCCGGATGACCCCGAAAAATGCAGTGT
CGTCATCATCCCAAGAGAAAACAGGCCGGGTCTGTTGTATGGGATCTTGGGAATTTTTGCACAAAGAGGAATCAATCTGA
CGCGTATCGAGTCCCGGCCGTCGAAGGAGGGGATCGGAAGATATGTCTTCTTCATCGATTTTGAGACCGATCCCGGCTGG
CAGGAGACGATCACGGAGTTGAAAAAAATCACCGGCGTCAAGGAACTGGGCTGCTATAGAAAAAAGGATTACGTATAA

Upstream 100 bases:

>100_bases
TCGCCGCGTTCTTCACGGAAAACAGCGATGCGTTCAAAGCCTATATTCCCCAGGCAACGGAAGAGACCGATCTTATGATA
AATACACTGGTGAAGATGAA

Downstream 100 bases:

>100_bases
TCAGGTGTCGAATACAGAAATACTGATACGGATATCTCACCGGGCACAAGTAGAGATATCTCCTTTATTATTCCTCTATT
ATTATAGTGCTAAAGACGGG

Product: prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTLAVLGPKGTFSCELAEKIRNENEEIILFPTIRDVFTAVLEKNIRGIVPVENSEAGGVGETLDGLLQTECRITAEYYMP
IRHFFVSRYSPDEISVIYTHPQSHEQCSIYLNGMKRASLIHTSSNAQSAKEASFISGSAAVTTESAAKLYDLPILQKDIQ
NSLNNTTRFLEISAGALDPDDPEKCSVVIIPRENRPGLLYGILGIFAQRGINLTRIESRPSKEGIGRYVFFIDFETDPGW
QETITELKKITGVKELGCYRKKDYV

Sequences:

>Translated_265_residues
MTLAVLGPKGTFSCELAEKIRNENEEIILFPTIRDVFTAVLEKNIRGIVPVENSEAGGVGETLDGLLQTECRITAEYYMP
IRHFFVSRYSPDEISVIYTHPQSHEQCSIYLNGMKRASLIHTSSNAQSAKEASFISGSAAVTTESAAKLYDLPILQKDIQ
NSLNNTTRFLEISAGALDPDDPEKCSVVIIPRENRPGLLYGILGIFAQRGINLTRIESRPSKEGIGRYVFFIDFETDPGW
QETITELKKITGVKELGCYRKKDYV
>Mature_264_residues
TLAVLGPKGTFSCELAEKIRNENEEIILFPTIRDVFTAVLEKNIRGIVPVENSEAGGVGETLDGLLQTECRITAEYYMPI
RHFFVSRYSPDEISVIYTHPQSHEQCSIYLNGMKRASLIHTSSNAQSAKEASFISGSAAVTTESAAKLYDLPILQKDIQN
SLNNTTRFLEISAGALDPDDPEKCSVVIIPRENRPGLLYGILGIFAQRGINLTRIESRPSKEGIGRYVFFIDFETDPGWQ
ETITELKKITGVKELGCYRKKDYV

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=277, Percent_Identity=26.7148014440433, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324013, Length=294, Percent_Identity=26.530612244898, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 29560; Mature: 29429

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00857 PREPHENATE_DEHYDR_1 ; PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAVLGPKGTFSCELAEKIRNENEEIILFPTIRDVFTAVLEKNIRGIVPVENSEAGGVG
CEEEEECCCCCEEHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCH
ETLDGLLQTECRITAEYYMPIRHFFVSRYSPDEISVIYTHPQSHEQCSIYLNGMKRASLI
HHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCEEEEE
HTSSNAQSAKEASFISGSAAVTTESAAKLYDLPILQKDIQNSLNNTTRFLEISAGALDPD
EECCCCCHHHHHHHCCCCCEEEHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCC
DPEKCSVVIIPRENRPGLLYGILGIFAQRGINLTRIESRPSKEGIGRYVFFIDFETDPGW
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEEEECCCCCH
QETITELKKITGVKELGCYRKKDYV
HHHHHHHHHHCCHHHHCCCHHCCCC
>Mature Secondary Structure 
TLAVLGPKGTFSCELAEKIRNENEEIILFPTIRDVFTAVLEKNIRGIVPVENSEAGGVG
EEEEECCCCCEEHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCH
ETLDGLLQTECRITAEYYMPIRHFFVSRYSPDEISVIYTHPQSHEQCSIYLNGMKRASLI
HHHHHHHHHHHEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCEEEEE
HTSSNAQSAKEASFISGSAAVTTESAAKLYDLPILQKDIQNSLNNTTRFLEISAGALDPD
EECCCCCHHHHHHHCCCCCEEEHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCC
DPEKCSVVIIPRENRPGLLYGILGIFAQRGINLTRIESRPSKEGIGRYVFFIDFETDPGW
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEEEECCCCCH
QETITELKKITGVKELGCYRKKDYV
HHHHHHHHHHCCHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]