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The map label for this gene is ttcA

Identifier: 124265747

GI number: 124265747

Start: 586478

End: 587419

Strand: Direct

Name: ttcA

Synonym: Mpe_A0554

Alternate gene names: 124265747

Gene position: 586478-587419 (Clockwise)

Preceding gene: 124265746

Following gene: 124265748

Centisome position: 14.5

GC content: 66.14

Gene sequence:

>942_bases
ATGAGTGCCGTCGTCATCGAGCCCGGCGTCACGCAACGTCAGGCCGGCAAGCCCGCCTTCGAGGCCAACAAGCTCAGCAA
ACGCCTGCACCGGCTGGTCGGCCAGGCCATCGGCGACTTCAACATGATCGAGCCCGGCGACAAGCTCATGGTCTGCGTGT
CGGGCGGCAAGGACAGCTACGCGCTGCTCGACATCCTGCTGAACCTGCAGCAGCGCGCGCCGATCGACTTCTCGCTCGTC
GCGGTCAACCTCGACCAGAAACAGCCCGACTTTCCCGAGCACGTGCTGCCCGAATACCTGGCGAGCCGCGGTGTGCCTTT
CCACATCGAGAACCAGGACACCTACTCCATCGTCAAGCGCGTGGTGCCAGAAGGCAAGACGATGTGCAGCCTGTGCTCGC
GGCTGAGGCGCGGCATCCTGTACCGCGTGGCCGGTGAGCTGGGGGCGACCAAGATCGCGCTCGGCCACCACCGTGACGAC
ATGCTGCAGACGCTGTTCCTCAACATGTTCTTCGGCGCCAAGCTCAAGGGCATGCCACCCAAACTGGTCAGCGACGACGG
CCGGCATGTCGTGATCCGCCCGCTGGCCTACGTGCCCGAGCACGACCTGGAAGCGTGGGCCGCGCACCGCCAGTTCCCCA
TCATCCCCTGCACCCTGTGCGGCAGCCAGGAGAACCTGCAGCGCAAGCAGATCGCGCAGATGCTGCGCGACTGGGAGCGC
CAGCACCCGGGTCGGCTCGACAACATGGCCAGCGCGCTGGCCAACGTGGTGCCTTCTCATCTGCAGGACCGGAACCTCTA
CCCCTTCACCACCCTCCAAGCCACCGGAAGAGCCGACGAACTGGGCGACAAGGCCTTCGATGACGACGAGACCTGTGCGC
CGCCCGGCGCGGCGTCGGTCATCGAGGCCGCGATGCCGATCCGTTGGCATGGGCAAGGCTGA

Upstream 100 bases:

>100_bases
CCCAGGTGCGCGCGGTGCGCGTGTCGACCGAGAAACCCGACGTCTACGCCGACTGCGCCGCGGTCGGCGTGGAGGTGTTC
CACATCAAGGAGAGCGACGC

Downstream 100 bases:

>100_bases
GTCAGGGCAAGGAGTCCGCATGCATCGAACCCGATCCGCCATGGCATCGCTGGCGGCATGCGCTGCGGCGCTGCTCGTCG
CCGGTTGCGCCAGCCTCAAC

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSAVVIEPGVTQRQAGKPAFEANKLSKRLHRLVGQAIGDFNMIEPGDKLMVCVSGGKDSYALLDILLNLQQRAPIDFSLV
AVNLDQKQPDFPEHVLPEYLASRGVPFHIENQDTYSIVKRVVPEGKTMCSLCSRLRRGILYRVAGELGATKIALGHHRDD
MLQTLFLNMFFGAKLKGMPPKLVSDDGRHVVIRPLAYVPEHDLEAWAAHRQFPIIPCTLCGSQENLQRKQIAQMLRDWER
QHPGRLDNMASALANVVPSHLQDRNLYPFTTLQATGRADELGDKAFDDDETCAPPGAASVIEAAMPIRWHGQG

Sequences:

>Translated_313_residues
MSAVVIEPGVTQRQAGKPAFEANKLSKRLHRLVGQAIGDFNMIEPGDKLMVCVSGGKDSYALLDILLNLQQRAPIDFSLV
AVNLDQKQPDFPEHVLPEYLASRGVPFHIENQDTYSIVKRVVPEGKTMCSLCSRLRRGILYRVAGELGATKIALGHHRDD
MLQTLFLNMFFGAKLKGMPPKLVSDDGRHVVIRPLAYVPEHDLEAWAAHRQFPIIPCTLCGSQENLQRKQIAQMLRDWER
QHPGRLDNMASALANVVPSHLQDRNLYPFTTLQATGRADELGDKAFDDDETCAPPGAASVIEAAMPIRWHGQG
>Mature_312_residues
SAVVIEPGVTQRQAGKPAFEANKLSKRLHRLVGQAIGDFNMIEPGDKLMVCVSGGKDSYALLDILLNLQQRAPIDFSLVA
VNLDQKQPDFPEHVLPEYLASRGVPFHIENQDTYSIVKRVVPEGKTMCSLCSRLRRGILYRVAGELGATKIALGHHRDDM
LQTLFLNMFFGAKLKGMPPKLVSDDGRHVVIRPLAYVPEHDLEAWAAHRQFPIIPCTLCGSQENLQRKQIAQMLRDWERQ
HPGRLDNMASALANVVPSHLQDRNLYPFTTLQATGRADELGDKAFDDDETCAPPGAASVIEAAMPIRWHGQG

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=287, Percent_Identity=64.808362369338, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI193211090, Length=234, Percent_Identity=32.0512820512821, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17540090, Length=230, Percent_Identity=28.2608695652174, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651972, Length=222, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_METPP (A2SD77)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019751.1
- ProteinModelPortal:   A2SD77
- SMR:   A2SD77
- STRING:   A2SD77
- GeneID:   4785240
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0554
- NMPDR:   fig|279263.3.peg.2794
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- PhylomeDB:   A2SD77
- ProtClustDB:   PRK10696
- BioCyc:   MPET420662:MPE_A0554-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 34759; Mature: 34628

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVVIEPGVTQRQAGKPAFEANKLSKRLHRLVGQAIGDFNMIEPGDKLMVCVSGGKDSY
CCEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHH
ALLDILLNLQQRAPIDFSLVAVNLDQKQPDFPEHVLPEYLASRGVPFHIENQDTYSIVKR
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHH
VVPEGKTMCSLCSRLRRGILYRVAGELGATKIALGHHRDDMLQTLFLNMFFGAKLKGMPP
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCC
KLVSDDGRHVVIRPLAYVPEHDLEAWAAHRQFPIIPCTLCGSQENLQRKQIAQMLRDWER
CCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
QHPGRLDNMASALANVVPSHLQDRNLYPFTTLQATGRADELGDKAFDDDETCAPPGAASV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCHHHH
IEAAMPIRWHGQG
HHHHCCEEECCCC
>Mature Secondary Structure 
SAVVIEPGVTQRQAGKPAFEANKLSKRLHRLVGQAIGDFNMIEPGDKLMVCVSGGKDSY
CEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHH
ALLDILLNLQQRAPIDFSLVAVNLDQKQPDFPEHVLPEYLASRGVPFHIENQDTYSIVKR
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHH
VVPEGKTMCSLCSRLRRGILYRVAGELGATKIALGHHRDDMLQTLFLNMFFGAKLKGMPP
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCC
KLVSDDGRHVVIRPLAYVPEHDLEAWAAHRQFPIIPCTLCGSQENLQRKQIAQMLRDWER
CCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
QHPGRLDNMASALANVVPSHLQDRNLYPFTTLQATGRADELGDKAFDDDETCAPPGAASV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCHHHH
IEAAMPIRWHGQG
HHHHCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA