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The map label for this gene is liaS [H]

Identifier: 124265670

GI number: 124265670

Start: 517830

End: 519296

Strand: Direct

Name: liaS [H]

Synonym: Mpe_A0477

Alternate gene names: 124265670

Gene position: 517830-519296 (Clockwise)

Preceding gene: 124265666

Following gene: 124265671

Centisome position: 12.8

GC content: 69.46

Gene sequence:

>1467_bases
GTGTATCGGGAGATCGCCCGCGCGAGATCGGGAGATCCTCCCGCTCGCCGCGATCGGGTCAGGAATGACGATTGCTGCCT
GCCCGGTGTGCATGCGATCGACGTAGCAAAGGAGACACCCATGGACTTGAGCAAGCGCCTCGTGGTCTGGCTCGCCTTGC
TCGCGGCGCTGTGGCTGGGGATCACGCTGGTCTACACCGCCGTGGCGCTGCGCTCGGGCATCCAGGACGAGATGCAGGGC
GCCAACGCGGTGGTGGAACTGCTGTCGACTGCTGCGGAGGCAAACCTCGGCGACGCGCACAGCCAACTCCGGCTGCAGGC
CCTGCTCGCGGAAGGGCATGCACGGCACCTGCGGGTGCGCTGGGCCGATGAGCCGAGTCAGGACAACCTGGAGCAGGTGT
CACTGCTCCACGACTACGCTCGCCGGCTGGCGCCCGCGGGAAAGCCCGTGGCCCAGCACCGCATCGCGATCGGTCCGCGA
ACGCTGATCGTCGAACCGGCGCCCGACAGCGAGATCGACGAGGTACTGGGCGATGTGCTGCCGTTGCTGCTGCTGTTGCT
CGTGGCCGGCGGGGCGATGCTGTTCGGCGTGTGGTGGATCGTGCGCCAAGCGCTGGCGCCGGCCAGCGTGCTGTGTGCCG
GACTGGCCCGGCTGCAGGCCGGCCAGCCGGAACCGGGCTTCCCGGCCTTGAGGCTGCGCGAGTACGCGGCCATCGCGCGG
GGCATCGACGAGCTCGCCGCCGGACTGGCCATGGCGCGATGCGAGCAGAGCCGGCTCACACAGCGACTGATCGACGTGCA
GGAGCGCGAGCGGCGCAGGCTGGCGAGCGACCTGCACGACGAGTTCGGCCAGCATCTGACCGCCATGTCGGCCACGGCTT
CGCTGCTTCAGCGCCATGCCGCACGGTTTGATCCGGTAGAGCTGGCGCGCTGCGCGGACCAGATGGTCGCTTCGCTGGGA
TGCATCCGACTGCAGCTGCGCACGCTCCTGAGCCGGCTGCGGCCGCACGGGCTCGAGGCCGAAGGACTGGCGCACGAACT
GGCGGGCTTGATCGATCGGGCCCGGGCTCATCACCCAACGGTGACGATCCGGGCCGACATCGCCAGGCCGCTGCCGGCAC
TGCCAGAGGACGCCGCGCTCGCGCTCTACCGCAGCCTGCAGGAAGCGTTGACGAACGCCTACCGACACAGCGGCGCAGGC
GAAGTGCAGGTCCGTATCGATGTGCCGGCCGTGCAGCGTGTGCGGATGCGGGTGATCGACGATGGCTGCGGCCGCGCCAG
TGCGCTGCCGCGGGGTGGCCTCGGCCTGCTCGGCATGCGCGAGCGCCTGGCCATGGTGGGTGGCGGATTGCGACTGGTCG
ACAACGCCGGTCCTGGCCTGTGCGTCGAAGTCTGGGTGCCGGTGCCGGCCGTCGCCGTCACCGAGTCACTTGAGGGAGAA
ATGCTTGATACACGTCTTGCTGCTTGA

Upstream 100 bases:

>100_bases
GCTGCGACGGACAGGGCGTGACGGACGGGATGCTTGCTCATCGGTATCTCCTCATGTTGTGGTGTACGAGCGAGCCGGAG
TCTAGGAAGCGGGGTCCACG

Downstream 100 bases:

>100_bases
CGATCACGCGGTGGTGCGGGCGGGGTACCGGCAGCTCATCGATGCCGAGCCCGACATGTGCGTGGTCGCAGCCTGCGCCA
CCTCGGCTCAGGCCTGCGAG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MYREIARARSGDPPARRDRVRNDDCCLPGVHAIDVAKETPMDLSKRLVVWLALLAALWLGITLVYTAVALRSGIQDEMQG
ANAVVELLSTAAEANLGDAHSQLRLQALLAEGHARHLRVRWADEPSQDNLEQVSLLHDYARRLAPAGKPVAQHRIAIGPR
TLIVEPAPDSEIDEVLGDVLPLLLLLLVAGGAMLFGVWWIVRQALAPASVLCAGLARLQAGQPEPGFPALRLREYAAIAR
GIDELAAGLAMARCEQSRLTQRLIDVQERERRRLASDLHDEFGQHLTAMSATASLLQRHAARFDPVELARCADQMVASLG
CIRLQLRTLLSRLRPHGLEAEGLAHELAGLIDRARAHHPTVTIRADIARPLPALPEDAALALYRSLQEALTNAYRHSGAG
EVQVRIDVPAVQRVRMRVIDDGCGRASALPRGGLGLLGMRERLAMVGGGLRLVDNAGPGLCVEVWVPVPAVAVTESLEGE
MLDTRLAA

Sequences:

>Translated_488_residues
MYREIARARSGDPPARRDRVRNDDCCLPGVHAIDVAKETPMDLSKRLVVWLALLAALWLGITLVYTAVALRSGIQDEMQG
ANAVVELLSTAAEANLGDAHSQLRLQALLAEGHARHLRVRWADEPSQDNLEQVSLLHDYARRLAPAGKPVAQHRIAIGPR
TLIVEPAPDSEIDEVLGDVLPLLLLLLVAGGAMLFGVWWIVRQALAPASVLCAGLARLQAGQPEPGFPALRLREYAAIAR
GIDELAAGLAMARCEQSRLTQRLIDVQERERRRLASDLHDEFGQHLTAMSATASLLQRHAARFDPVELARCADQMVASLG
CIRLQLRTLLSRLRPHGLEAEGLAHELAGLIDRARAHHPTVTIRADIARPLPALPEDAALALYRSLQEALTNAYRHSGAG
EVQVRIDVPAVQRVRMRVIDDGCGRASALPRGGLGLLGMRERLAMVGGGLRLVDNAGPGLCVEVWVPVPAVAVTESLEGE
MLDTRLAA
>Mature_488_residues
MYREIARARSGDPPARRDRVRNDDCCLPGVHAIDVAKETPMDLSKRLVVWLALLAALWLGITLVYTAVALRSGIQDEMQG
ANAVVELLSTAAEANLGDAHSQLRLQALLAEGHARHLRVRWADEPSQDNLEQVSLLHDYARRLAPAGKPVAQHRIAIGPR
TLIVEPAPDSEIDEVLGDVLPLLLLLLVAGGAMLFGVWWIVRQALAPASVLCAGLARLQAGQPEPGFPALRLREYAAIAR
GIDELAAGLAMARCEQSRLTQRLIDVQERERRRLASDLHDEFGQHLTAMSATASLLQRHAARFDPVELARCADQMVASLG
CIRLQLRTLLSRLRPHGLEAEGLAHELAGLIDRARAHHPTVTIRADIARPLPALPEDAALALYRSLQEALTNAYRHSGAG
EVQVRIDVPAVQRVRMRVIDDGCGRASALPRGGLGLLGMRERLAMVGGGLRLVDNAGPGLCVEVWVPVPAVAVTESLEGE
MLDTRLAA

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=235, Percent_Identity=28.936170212766, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787474, Length=212, Percent_Identity=30.188679245283, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52899; Mature: 52899

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYREIARARSGDPPARRDRVRNDDCCLPGVHAIDVAKETPMDLSKRLVVWLALLAALWLG
CCHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ITLVYTAVALRSGIQDEMQGANAVVELLSTAAEANLGDAHSQLRLQALLAEGHARHLRVR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEE
WADEPSQDNLEQVSLLHDYARRLAPAGKPVAQHRIAIGPRTLIVEPAPDSEIDEVLGDVL
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCCEEEECCCCCHHHHHHHHHHH
PLLLLLLVAGGAMLFGVWWIVRQALAPASVLCAGLARLQAGQPEPGFPALRLREYAAIAR
HHHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GIDELAAGLAMARCEQSRLTQRLIDVQERERRRLASDLHDEFGQHLTAMSATASLLQRHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARFDPVELARCADQMVASLGCIRLQLRTLLSRLRPHGLEAEGLAHELAGLIDRARAHHPT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCE
VTIRADIARPLPALPEDAALALYRSLQEALTNAYRHSGAGEVQVRIDVPAVQRVRMRVID
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHH
DGCGRASALPRGGLGLLGMRERLAMVGGGLRLVDNAGPGLCVEVWVPVPAVAVTESLEGE
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCHHHHHHHHCCH
MLDTRLAA
HHHHHHCC
>Mature Secondary Structure
MYREIARARSGDPPARRDRVRNDDCCLPGVHAIDVAKETPMDLSKRLVVWLALLAALWLG
CCHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ITLVYTAVALRSGIQDEMQGANAVVELLSTAAEANLGDAHSQLRLQALLAEGHARHLRVR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEE
WADEPSQDNLEQVSLLHDYARRLAPAGKPVAQHRIAIGPRTLIVEPAPDSEIDEVLGDVL
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCCEEEECCCCCHHHHHHHHHHH
PLLLLLLVAGGAMLFGVWWIVRQALAPASVLCAGLARLQAGQPEPGFPALRLREYAAIAR
HHHHHHHHHCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GIDELAAGLAMARCEQSRLTQRLIDVQERERRRLASDLHDEFGQHLTAMSATASLLQRHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARFDPVELARCADQMVASLGCIRLQLRTLLSRLRPHGLEAEGLAHELAGLIDRARAHHPT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCE
VTIRADIARPLPALPEDAALALYRSLQEALTNAYRHSGAGEVQVRIDVPAVQRVRMRVID
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHH
DGCGRASALPRGGLGLLGMRERLAMVGGGLRLVDNAGPGLCVEVWVPVPAVAVTESLEGE
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCHHHHHHHHCCH
MLDTRLAA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]