Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 124265650

GI number: 124265650

Start: 495044

End: 496555

Strand: Direct

Name: murE [H]

Synonym: Mpe_A0457

Alternate gene names: 124265650

Gene position: 495044-496555 (Clockwise)

Preceding gene: 124265649

Following gene: 124265651

Centisome position: 12.24

GC content: 74.47

Gene sequence:

>1512_bases
ATGCTCACCGCGTTGCGTTCGCCCGCCGCCGCGGCCCGCTGGCTCCAGGAATGGACCACCGGCACGCTGCGCACCGACAG
CCGCCTGGTGCAGCGCGGCGATGCCTTCATTGCCTGGCCCGGCCATGCCACCGATGGCCGCCGCTTCGTGAAGGCCGCGC
TGGCCGCCGGTGCGGCGACCTGCCTGGTCGAGGCCGAGGGGGTGGACGCCCACGGCTTCGACGACGCACGCATCGGCGCG
CTGCCCGGGCTCAAGGCCGCCACCGGTGCGATCGCCGACGCCTACTTCGGCCAGCCGTCGCAGCAGCTGCGGGTGGTGGC
CACCACCGGCACCAACGGCAAGACCTCCACCGCGTGGTGGACCGCGCAGGCGCTGAGCCTGCTGGGCCGGCGCTGCGGCG
TGGTCGGCACGCTCGGCATCGGCGAGCCGCCGCTGCCCGGCCGCGAGACGCACATCGACTTCACCGGCCTCACCACGCCC
GACCCGGTCACGCTGCACGGCGGGCTGCGCCGCATGGCCGACGCCGGCTTCGAGGCCTGCGCGATCGAGGCCTCGTCGAT
CGGCATCGTCGAGCACCGGCTCGCGGCACTGCGCATCGAAGTGGCGCTGTTCACCAACTTCACCCGCGACCACCTCGACT
ACCACGGCAGCATGGACGCCTACTGGGCCGCCAAGCGCGTGCTGTTCGGCTGGCCCGGGCTGAAGGCCGCTGCGGTCAAC
CTCGACGATGCCCAGGGCGCCGAACTGGCACGCAGCCTGGCCGGGCACGTGCCCGAACTCTGGACCTATTCGCTCGAGCG
CGACGACGTGCGGCTGCGCGCGCGCGACCTCGGCTATGTCGACGGCGGGCTCGCGTTCACGCTCTACGAGGGCGGCACTG
CCCTGCCGGTGCGCAGCCGCCTGATCGGCGACTACAACGCCGCCAACCTGCTGGCCGTGATCGGCGGCCTGCGCGCGCTG
GGCGTGCCGCTGGCCGACGCGGTGCGCGTGGTGCCGCAGCTCACGCCGGTGCCGGGCCGCATGCAGAAGGTGGACGGCGA
CGCGGGCGTCGCCCCGCAGCCGGAGGTGGTGGTCGACTACGCGCACACCCCCGACGCGCTGGAGAAGGCGCTCGGTGCGC
TGCGTCCGCTGGCCGCGGCGCGCGGCGGCCGGTTGCTGTGCGTGTTCGGCTGCGGCGGCAACCGCGACGCCAGCAAGCGC
CCGCTGATGGCCGCGGTCGCCGAGCGACTGGCCGACCGCGTGATCGCGACCAGCGACAACCCGCGCGACGAGGCGCCGGC
GGCGATCCTGGTCGATGTCGCCGCAGGCTTCGCGGCGCCGGCCGCCGTCGAGATGATCGAAGACCGCCGCGCGGCGATCG
CCCGCGCGATCGGCAGCGCCGAGGCCCGCGACGTGCTGCTGATCGCCGGCAAGGGCCACGAGGACTACCAGGAGATCGCC
GGCGTCAAGCAGCCGTTCTCCGATGTGGCCGAGGCGCGGCAGGCGCTGGCGGCGCGCGGGTTGGCGTCATGA

Upstream 100 bases:

>100_bases
TGGTGCAGAAGACGCTGCATGCGATGGGTGTTTCGCCCGATCTCGACGTCAAGCCGCAGATCGTGGCGCGCCACGAGGAC
GCTGTGGAGGAGAGCTTCTG

Downstream 100 bases:

>100_bases
GCGTGATGATGGACTTGCGGTTGGCGCATGCGCTGCTGCCGGGCTCGACGCTCGTCGGCGACCCGGCCACGCCGCTGTTG
CGCGTGCACAGCGATACCCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MLTALRSPAAAARWLQEWTTGTLRTDSRLVQRGDAFIAWPGHATDGRRFVKAALAAGAATCLVEAEGVDAHGFDDARIGA
LPGLKAATGAIADAYFGQPSQQLRVVATTGTNGKTSTAWWTAQALSLLGRRCGVVGTLGIGEPPLPGRETHIDFTGLTTP
DPVTLHGGLRRMADAGFEACAIEASSIGIVEHRLAALRIEVALFTNFTRDHLDYHGSMDAYWAAKRVLFGWPGLKAAAVN
LDDAQGAELARSLAGHVPELWTYSLERDDVRLRARDLGYVDGGLAFTLYEGGTALPVRSRLIGDYNAANLLAVIGGLRAL
GVPLADAVRVVPQLTPVPGRMQKVDGDAGVAPQPEVVVDYAHTPDALEKALGALRPLAAARGGRLLCVFGCGGNRDASKR
PLMAAVAERLADRVIATSDNPRDEAPAAILVDVAAGFAAPAAVEMIEDRRAAIARAIGSAEARDVLLIAGKGHEDYQEIA
GVKQPFSDVAEARQALAARGLAS

Sequences:

>Translated_503_residues
MLTALRSPAAAARWLQEWTTGTLRTDSRLVQRGDAFIAWPGHATDGRRFVKAALAAGAATCLVEAEGVDAHGFDDARIGA
LPGLKAATGAIADAYFGQPSQQLRVVATTGTNGKTSTAWWTAQALSLLGRRCGVVGTLGIGEPPLPGRETHIDFTGLTTP
DPVTLHGGLRRMADAGFEACAIEASSIGIVEHRLAALRIEVALFTNFTRDHLDYHGSMDAYWAAKRVLFGWPGLKAAAVN
LDDAQGAELARSLAGHVPELWTYSLERDDVRLRARDLGYVDGGLAFTLYEGGTALPVRSRLIGDYNAANLLAVIGGLRAL
GVPLADAVRVVPQLTPVPGRMQKVDGDAGVAPQPEVVVDYAHTPDALEKALGALRPLAAARGGRLLCVFGCGGNRDASKR
PLMAAVAERLADRVIATSDNPRDEAPAAILVDVAAGFAAPAAVEMIEDRRAAIARAIGSAEARDVLLIAGKGHEDYQEIA
GVKQPFSDVAEARQALAARGLAS
>Mature_503_residues
MLTALRSPAAAARWLQEWTTGTLRTDSRLVQRGDAFIAWPGHATDGRRFVKAALAAGAATCLVEAEGVDAHGFDDARIGA
LPGLKAATGAIADAYFGQPSQQLRVVATTGTNGKTSTAWWTAQALSLLGRRCGVVGTLGIGEPPLPGRETHIDFTGLTTP
DPVTLHGGLRRMADAGFEACAIEASSIGIVEHRLAALRIEVALFTNFTRDHLDYHGSMDAYWAAKRVLFGWPGLKAAAVN
LDDAQGAELARSLAGHVPELWTYSLERDDVRLRARDLGYVDGGLAFTLYEGGTALPVRSRLIGDYNAANLLAVIGGLRAL
GVPLADAVRVVPQLTPVPGRMQKVDGDAGVAPQPEVVVDYAHTPDALEKALGALRPLAAARGGRLLCVFGCGGNRDASKR
PLMAAVAERLADRVIATSDNPRDEAPAAILVDVAAGFAAPAAVEMIEDRRAAIARAIGSAEARDVLLIAGKGHEDYQEIA
GVKQPFSDVAEARQALAARGLAS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=496, Percent_Identity=40.9274193548387, Blast_Score=285, Evalue=5e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52784; Mature: 52784

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTALRSPAAAARWLQEWTTGTLRTDSRLVQRGDAFIAWPGHATDGRRFVKAALAAGAAT
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCEE
CLVEAEGVDAHGFDDARIGALPGLKAATGAIADAYFGQPSQQLRVVATTGTNGKTSTAWW
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHH
TAQALSLLGRRCGVVGTLGIGEPPLPGRETHIDFTGLTTPDPVTLHGGLRRMADAGFEAC
HHHHHHHHHHHCCEEEECCCCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHCCCCEE
AIEASSIGIVEHRLAALRIEVALFTNFTRDHLDYHGSMDAYWAAKRVLFGWPGLKAAAVN
EEECCCCCHHHHHHHHHEEEEHHHHCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEC
LDDAQGAELARSLAGHVPELWTYSLERDDVRLRARDLGYVDGGLAFTLYEGGTALPVRSR
CCCCCHHHHHHHHHCCCCHHHEEECCCCCEEEEHHHCCEECCCEEEEEECCCCCCCHHHH
LIGDYNAANLLAVIGGLRALGVPLADAVRVVPQLTPVPGRMQKVDGDAGVAPQPEVVVDY
HHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHEECCCCCCCCCCCCEEEEE
AHTPDALEKALGALRPLAAARGGRLLCVFGCGGNRDASKRPLMAAVAERLADRVIATSDN
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEECCCC
PRDEAPAAILVDVAAGFAAPAAVEMIEDRRAAIARAIGSAEARDVLLIAGKGHEDYQEIA
CCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
GVKQPFSDVAEARQALAARGLAS
CCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLTALRSPAAAARWLQEWTTGTLRTDSRLVQRGDAFIAWPGHATDGRRFVKAALAAGAAT
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCEE
CLVEAEGVDAHGFDDARIGALPGLKAATGAIADAYFGQPSQQLRVVATTGTNGKTSTAWW
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHH
TAQALSLLGRRCGVVGTLGIGEPPLPGRETHIDFTGLTTPDPVTLHGGLRRMADAGFEAC
HHHHHHHHHHHCCEEEECCCCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHCCCCEE
AIEASSIGIVEHRLAALRIEVALFTNFTRDHLDYHGSMDAYWAAKRVLFGWPGLKAAAVN
EEECCCCCHHHHHHHHHEEEEHHHHCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEC
LDDAQGAELARSLAGHVPELWTYSLERDDVRLRARDLGYVDGGLAFTLYEGGTALPVRSR
CCCCCHHHHHHHHHCCCCHHHEEECCCCCEEEEHHHCCEECCCEEEEEECCCCCCCHHHH
LIGDYNAANLLAVIGGLRALGVPLADAVRVVPQLTPVPGRMQKVDGDAGVAPQPEVVVDY
HHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHEECCCCCCCCCCCCEEEEE
AHTPDALEKALGALRPLAAARGGRLLCVFGCGGNRDASKRPLMAAVAERLADRVIATSDN
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEECCCC
PRDEAPAAILVDVAAGFAAPAAVEMIEDRRAAIARAIGSAEARDVLLIAGKGHEDYQEIA
CCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
GVKQPFSDVAEARQALAARGLAS
CCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA