| Definition | Methylibium petroleiphilum PM1 chromosome, complete genome. |
|---|---|
| Accession | NC_008825 |
| Length | 4,044,195 |
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The map label for this gene is murF [H]
Identifier: 124265651
GI number: 124265651
Start: 496552
End: 497964
Strand: Direct
Name: murF [H]
Synonym: Mpe_A0458
Alternate gene names: 124265651
Gene position: 496552-497964 (Clockwise)
Preceding gene: 124265650
Following gene: 124265652
Centisome position: 12.28
GC content: 74.31
Gene sequence:
>1413_bases ATGAGCGTGATGATGGACTTGCGGTTGGCGCATGCGCTGCTGCCGGGCTCGACGCTCGTCGGCGACCCGGCCACGCCGCT GTTGCGCGTGCACAGCGATACCCGCACGCTGCGCGCCGGTGACCTGTTCGTTGCGCTCAAGGGCGAGCGCTTCGACGCGC ACGACTTCCTGCCGCAGGCGCGTGCCGCCGGCGCAGTGGCGGCGATCGCCGAGCACGGCCTCGCCGAGGCCGGCCTGGCC GGGCTGCACGTGGCCGACAGCCGCGCCGCGCTCGGCACGCTGGGCGCGAACTGGCGCGCCCGCATGAGCCTGCCGCTGAT CGCTGTGACCGGCAGCAACGGCAAGACCACCGTCACGCAGATGATCGCGAGCATCCTGCGCGCCTGGGTCGGCGACGCGG CATTCGCGACCGAGGGCAACTTCAACAACGACATCGGCGTGCCGCTCACGCTGCTGCGGTTGCGCCAGGACGCCGACACC TGGCACCGCGCGGCGGTCGTCGAGCTGGGCATGAACCACGTCGGCGAGATCGCCCAGCTCGCGCGCATGGCCGCGCCGAC GGTCGCGCTGGTCAACAACGCCCAGCGCGAGCACCAGGAATTCATGGCCAGCGTCGAGGCGGTGGCCCGCGAGAACGGCG CGGTGATCGAGGTGCTGGGTGCCAACGGCACGGCGGTCTTCCCGTCCGACGACGCGCATGCCGCGGTATGGCAGGCGCTG GCCGGCACGCGGCCGACGTTGACGTTCTCGCTCGAAGGTCCGTCCGACTTGGTGGCCGAGGCGGTGTGGAGCGGCGACCA TTGGGCGGCGGTGCTGCACACGCCGGCCGGCGCCACCGCGATCCGCCTGCGCGTGGCCGGCTGGCACAACGTGAAGAACG CGCTCGCCGCGACCGCCTGCGCCCTGGCGGCGGGCTGCCCGCTGGAGGCGGTGGCGCGTGGCCTCGAGGCCTTCGAGCCG GTGAAGGGCCGCTCGCAGGTCAAGGCCCTGCAGCGCGGCGGCCGCGCGGTGACGCTGGTGGACGACAGCTACAACGCCAA CCCCGACTCGGTGCGCGCGGCGATCGACGTGCTGGCCACGCTGCCCGGCCCGCGCTGGCTGGTGCTGGGCGACATGGGCG AGGTCGGCGACCAGGGCCCGGCCTTCCACGCCGAGGTCGGCCGCTATGCGGCCGCTTGCGGCATCGACGCGCTGTGGACC GCCGGCGCCGCCAGCGCCGCCGCCGCCGCCGCCTTCGGCCCGGCCGCACGGGCCCACGCGAGCGTGGCCGAGCTGATCGC GGCGCTGGGGCCGGACACGCACGCCGCCCCGGAGGCCGCGTCCATCGTCGTCAAGGGCTCGCGCTTCATGAAGATGGAGC AGGTGATCGCCGCGCTGCAGGCCGCTGGCGCCGCATCGCCCACCCCGCACTGA
Upstream 100 bases:
>100_bases GCAAGGGCCACGAGGACTACCAGGAGATCGCCGGCGTCAAGCAGCCGTTCTCCGATGTGGCCGAGGCGCGGCAGGCGCTG GCGGCGCGCGGGTTGGCGTC
Downstream 100 bases:
>100_bases ACCGAAGGCACCGCAACTTCATGCTGATCTCCTTGTCGCAATGGCTGCAACTGCTCTACCCCGAGCAGCTCGGTTTCCTG CGCGTGTTTCAGTACCTCAC
Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 470; Mature: 469
Protein sequence:
>470_residues MSVMMDLRLAHALLPGSTLVGDPATPLLRVHSDTRTLRAGDLFVALKGERFDAHDFLPQARAAGAVAAIAEHGLAEAGLA GLHVADSRAALGTLGANWRARMSLPLIAVTGSNGKTTVTQMIASILRAWVGDAAFATEGNFNNDIGVPLTLLRLRQDADT WHRAAVVELGMNHVGEIAQLARMAAPTVALVNNAQREHQEFMASVEAVARENGAVIEVLGANGTAVFPSDDAHAAVWQAL AGTRPTLTFSLEGPSDLVAEAVWSGDHWAAVLHTPAGATAIRLRVAGWHNVKNALAATACALAAGCPLEAVARGLEAFEP VKGRSQVKALQRGGRAVTLVDDSYNANPDSVRAAIDVLATLPGPRWLVLGDMGEVGDQGPAFHAEVGRYAAACGIDALWT AGAASAAAAAAFGPAARAHASVAELIAALGPDTHAAPEAASIVVKGSRFMKMEQVIAALQAAGAASPTPH
Sequences:
>Translated_470_residues MSVMMDLRLAHALLPGSTLVGDPATPLLRVHSDTRTLRAGDLFVALKGERFDAHDFLPQARAAGAVAAIAEHGLAEAGLA GLHVADSRAALGTLGANWRARMSLPLIAVTGSNGKTTVTQMIASILRAWVGDAAFATEGNFNNDIGVPLTLLRLRQDADT WHRAAVVELGMNHVGEIAQLARMAAPTVALVNNAQREHQEFMASVEAVARENGAVIEVLGANGTAVFPSDDAHAAVWQAL AGTRPTLTFSLEGPSDLVAEAVWSGDHWAAVLHTPAGATAIRLRVAGWHNVKNALAATACALAAGCPLEAVARGLEAFEP VKGRSQVKALQRGGRAVTLVDDSYNANPDSVRAAIDVLATLPGPRWLVLGDMGEVGDQGPAFHAEVGRYAAACGIDALWT AGAASAAAAAAFGPAARAHASVAELIAALGPDTHAAPEAASIVVKGSRFMKMEQVIAALQAAGAASPTPH >Mature_469_residues SVMMDLRLAHALLPGSTLVGDPATPLLRVHSDTRTLRAGDLFVALKGERFDAHDFLPQARAAGAVAAIAEHGLAEAGLAG LHVADSRAALGTLGANWRARMSLPLIAVTGSNGKTTVTQMIASILRAWVGDAAFATEGNFNNDIGVPLTLLRLRQDADTW HRAAVVELGMNHVGEIAQLARMAAPTVALVNNAQREHQEFMASVEAVARENGAVIEVLGANGTAVFPSDDAHAAVWQALA GTRPTLTFSLEGPSDLVAEAVWSGDHWAAVLHTPAGATAIRLRVAGWHNVKNALAATACALAAGCPLEAVARGLEAFEPV KGRSQVKALQRGGRAVTLVDDSYNANPDSVRAAIDVLATLPGPRWLVLGDMGEVGDQGPAFHAEVGRYAAACGIDALWTA GAASAAAAAAFGPAARAHASVAELIAALGPDTHAAPEAASIVVKGSRFMKMEQVIAALQAAGAASPTPH
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=438, Percent_Identity=42.0091324200913, Blast_Score=248, Evalue=9e-67,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 48375; Mature: 48244
Theoretical pI: Translated: 6.41; Mature: 6.41
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSVMMDLRLAHALLPGSTLVGDPATPLLRVHSDTRTLRAGDLFVALKGERFDAHDFLPQA CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCEEEEEECCCCCHHHCCHHH RAAGAVAAIAEHGLAEAGLAGLHVADSRAALGTLGANWRARMSLPLIAVTGSNGKTTVTQ HHHHHHHHHHHCCHHHCCCCCEEECCCCHHHHHCCCCCEEEECCEEEEEECCCCCHHHHH MIASILRAWVGDAAFATEGNFNNDIGVPLTLLRLRQDADTWHRAAVVELGMNHVGEIAQL HHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH ARMAAPTVALVNNAQREHQEFMASVEAVARENGAVIEVLGANGTAVFPSDDAHAAVWQAL HHHHCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCHHHHHHHHH AGTRPTLTFSLEGPSDLVAEAVWSGDHWAAVLHTPAGATAIRLRVAGWHNVKNALAATAC CCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHH ALAAGCPLEAVARGLEAFEPVKGRSQVKALQRGGRAVTLVDDSYNANPDSVRAAIDVLAT HHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH LPGPRWLVLGDMGEVGDQGPAFHAEVGRYAAACGIDALWTAGAASAAAAAAFGPAARAHA CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHCCCHHHHHH SVAELIAALGPDTHAAPEAASIVVKGSRFMKMEQVIAALQAAGAASPTPH HHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCC >Mature Secondary Structure SVMMDLRLAHALLPGSTLVGDPATPLLRVHSDTRTLRAGDLFVALKGERFDAHDFLPQA CHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCEEEEEECCCCCHHHCCHHH RAAGAVAAIAEHGLAEAGLAGLHVADSRAALGTLGANWRARMSLPLIAVTGSNGKTTVTQ HHHHHHHHHHHCCHHHCCCCCEEECCCCHHHHHCCCCCEEEECCEEEEEECCCCCHHHHH MIASILRAWVGDAAFATEGNFNNDIGVPLTLLRLRQDADTWHRAAVVELGMNHVGEIAQL HHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH ARMAAPTVALVNNAQREHQEFMASVEAVARENGAVIEVLGANGTAVFPSDDAHAAVWQAL HHHHCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCHHHHHHHHH AGTRPTLTFSLEGPSDLVAEAVWSGDHWAAVLHTPAGATAIRLRVAGWHNVKNALAATAC CCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHH ALAAGCPLEAVARGLEAFEPVKGRSQVKALQRGGRAVTLVDDSYNANPDSVRAAIDVLAT HHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH LPGPRWLVLGDMGEVGDQGPAFHAEVGRYAAACGIDALWTAGAASAAAAAAFGPAARAHA CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHCCCHHHHHH SVAELIAALGPDTHAAPEAASIVVKGSRFMKMEQVIAALQAAGAASPTPH HHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]