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The map label for this gene is phbI [H]

Identifier: 124265519

GI number: 124265519

Start: 350084

End: 351919

Strand: Direct

Name: phbI [H]

Synonym: Mpe_A0326

Alternate gene names: 124265519

Gene position: 350084-351919 (Clockwise)

Preceding gene: 124265517

Following gene: 124265520

Centisome position: 8.66

GC content: 67.27

Gene sequence:

>1836_bases
ATGTGCGCCGTGGTAACCCCTGCCGGCAGCTGGACGGGCCGCTGCTACGATCGATTCATGAGCCTGCAGATGTTCGGAAT
TCCGGTCTCGCGCGGCGTGGCCATTGGCCGCGCCGTGCTGGTCGCGTCCAGTCGCGTGGACGTGGCCCACTACTTCATCG
AGCCGGCGCAGGTCGAGCGCGAGATCGCGCGGCTGCTGCAGGCGCGCGACGCGGTGGCGGCCGAGCTCGGCGGCCTCCAG
CGCGACCTGCCAGAGGACGCGCCCGCGGAACTGTCCGCGCTGCTCGACGTGCACCTGATGCTGCTGCACGACGAGGCGCT
GACCGGCGCCACGTCGCAATGGGTGCACGAGCGGCACTACAACGCGGAATGGGCGCTGTCGGCCCAGCTGGAAGTGCTGG
CGCGGCATTTCGACGACATGGAAAATGATTACCTGCGTGAGCGCAAGGCCGACCTTGAACAGGTGGTCGAGCGCTTGTTG
CGTGTGCTGATGCACGACAGCAGCGCCGTACCGCCGTCGATCGGAGTCAACCCGCGCGACTTCGCCGGCGAGGACCCGCT
GGTGCTGGTGGCCAACGACATCGCGCCGGCCGACATGCTGCAATTCAAGCGCAGCGTCTTCACCGGTTTTGTCACCGACG
TCGGCGGCAAGACCTCGCACACGGCCATCGTCGCCCGCAGCCTCGACATTCCGGCGGTGGTGGGGGCCCGTGAGGCCAGC
CGCATCATCCGGCAGGATGACTGGGTGGTGATCGACGGTGACGCCGGAGTCGTGATTGTCGATCCGTCGTCGATCGTGCT
GGAGGAATACCGCTTTCGCCAGCGGCAGAGCGAACTGGAGCGTGTGCGGCTTACGCGCCTGCGGCATACACCCGCCGTCA
CGCTGGACGGTGAGCGGGTCGAGCTGTTTGCCAATATCGAGCTCCCTGGCGACGCGGCCGCGGCACTGGAGGCCGGGGCG
GTCGGCGTCGGGCTGTTTCGTAGCGAATTCCTCTTCATGAACCGGACCGACGACCTGCCCGGCGAGGATGAGCAGTACCA
GGCGTACTGCGCAGTGGTCGATGCGATGAAAGGTCTGCCGGTGACGATCCGGACGGTCGATATTGGCGCCGACAAGCCGC
TCGACCGGATGTCGGCCCACGAACTGCGGCACGAGCATGCGCTCAACCCCGCGCTCGGCTTGCGCGCGATCCGCTGGAGC
CTGTCCGAGCCCAGCATGTTCCGCCAGCAGCTGCGCGCCATCCTGCGCGCCAGTGCGCATGGTCAGGTGCGTTTGCTGGT
GCCGATGCTCGCGCACGAGTCCGAGATTCGCGGCACTTTCGATGCGCTGGCGCGCGCGAAGCAGCAGTTGACGGAATCAG
GGCGCGCGTTCGGCGATGTCCAGGTGGGCGCGATGATCGAAGTGCCCGCCGCGGCGTTGATGATCGATCGCTTTCTCGAT
GCCTTCGATTTCGTGTCGCTCGGCACCAATGATCTGATCCAGTACACGCTGGCCATCGACCGAGCCGATGAGGCCGTCGC
CCACCTGTACGATCCCTGGCACCCCGCCGTGCTGGAGCTCGTCGCCAGAACCATTCGCGCAGCAAGAGCGCGCGGCAGGG
CGGTCAGCGTGTGCGGAGAGATGGCCGGTGACCCGAGCTTCACTTCTGTCCTGCTGGCCATGGGCCTGCGCAGCTTTTCC
ATGCACCCGTCGCAGATCGCTGCGATCAAGCAGCAGATCCTGCGCACGGACACGCGGCGGCTGAGCGATCTGCTATTGGG
GGCCAGGAGCGACGCTCCGACGTTCACGCCGCTGCGGAACGGCGGGGGCGTGGCGGCGACGCCCCCGCGGCCTTGA

Upstream 100 bases:

>100_bases
CGCTCCTTGTTCGGCAATGGCGACGAGCTTGAGTCAGCCGCGTGACGACGCGATGTAGCTCCGATGACGCGCCGATGACA
CGCGCACAGGGTCCGGGTCG

Downstream 100 bases:

>100_bases
AGACTGCATCAACAATTTCGTCGACGACCTTGCGCGGCGGAAAAACTTCGTGCTACAGTAGCTGTCTTCGCTGAACACAG
CGCCTCGCCGGAAACGGCAG

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MCAVVTPAGSWTGRCYDRFMSLQMFGIPVSRGVAIGRAVLVASSRVDVAHYFIEPAQVEREIARLLQARDAVAAELGGLQ
RDLPEDAPAELSALLDVHLMLLHDEALTGATSQWVHERHYNAEWALSAQLEVLARHFDDMENDYLRERKADLEQVVERLL
RVLMHDSSAVPPSIGVNPRDFAGEDPLVLVANDIAPADMLQFKRSVFTGFVTDVGGKTSHTAIVARSLDIPAVVGAREAS
RIIRQDDWVVIDGDAGVVIVDPSSIVLEEYRFRQRQSELERVRLTRLRHTPAVTLDGERVELFANIELPGDAAAALEAGA
VGVGLFRSEFLFMNRTDDLPGEDEQYQAYCAVVDAMKGLPVTIRTVDIGADKPLDRMSAHELRHEHALNPALGLRAIRWS
LSEPSMFRQQLRAILRASAHGQVRLLVPMLAHESEIRGTFDALARAKQQLTESGRAFGDVQVGAMIEVPAAALMIDRFLD
AFDFVSLGTNDLIQYTLAIDRADEAVAHLYDPWHPAVLELVARTIRAARARGRAVSVCGEMAGDPSFTSVLLAMGLRSFS
MHPSQIAAIKQQILRTDTRRLSDLLLGARSDAPTFTPLRNGGGVAATPPRP

Sequences:

>Translated_611_residues
MCAVVTPAGSWTGRCYDRFMSLQMFGIPVSRGVAIGRAVLVASSRVDVAHYFIEPAQVEREIARLLQARDAVAAELGGLQ
RDLPEDAPAELSALLDVHLMLLHDEALTGATSQWVHERHYNAEWALSAQLEVLARHFDDMENDYLRERKADLEQVVERLL
RVLMHDSSAVPPSIGVNPRDFAGEDPLVLVANDIAPADMLQFKRSVFTGFVTDVGGKTSHTAIVARSLDIPAVVGAREAS
RIIRQDDWVVIDGDAGVVIVDPSSIVLEEYRFRQRQSELERVRLTRLRHTPAVTLDGERVELFANIELPGDAAAALEAGA
VGVGLFRSEFLFMNRTDDLPGEDEQYQAYCAVVDAMKGLPVTIRTVDIGADKPLDRMSAHELRHEHALNPALGLRAIRWS
LSEPSMFRQQLRAILRASAHGQVRLLVPMLAHESEIRGTFDALARAKQQLTESGRAFGDVQVGAMIEVPAAALMIDRFLD
AFDFVSLGTNDLIQYTLAIDRADEAVAHLYDPWHPAVLELVARTIRAARARGRAVSVCGEMAGDPSFTSVLLAMGLRSFS
MHPSQIAAIKQQILRTDTRRLSDLLLGARSDAPTFTPLRNGGGVAATPPRP
>Mature_611_residues
MCAVVTPAGSWTGRCYDRFMSLQMFGIPVSRGVAIGRAVLVASSRVDVAHYFIEPAQVEREIARLLQARDAVAAELGGLQ
RDLPEDAPAELSALLDVHLMLLHDEALTGATSQWVHERHYNAEWALSAQLEVLARHFDDMENDYLRERKADLEQVVERLL
RVLMHDSSAVPPSIGVNPRDFAGEDPLVLVANDIAPADMLQFKRSVFTGFVTDVGGKTSHTAIVARSLDIPAVVGAREAS
RIIRQDDWVVIDGDAGVVIVDPSSIVLEEYRFRQRQSELERVRLTRLRHTPAVTLDGERVELFANIELPGDAAAALEAGA
VGVGLFRSEFLFMNRTDDLPGEDEQYQAYCAVVDAMKGLPVTIRTVDIGADKPLDRMSAHELRHEHALNPALGLRAIRWS
LSEPSMFRQQLRAILRASAHGQVRLLVPMLAHESEIRGTFDALARAKQQLTESGRAFGDVQVGAMIEVPAAALMIDRFLD
AFDFVSLGTNDLIQYTLAIDRADEAVAHLYDPWHPAVLELVARTIRAARARGRAVSVCGEMAGDPSFTSVLLAMGLRSFS
MHPSQIAAIKQQILRTDTRRLSDLLLGARSDAPTFTPLRNGGGVAATPPRP

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=554, Percent_Identity=38.8086642599278, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI48994992, Length=505, Percent_Identity=34.6534653465347, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1788726, Length=569, Percent_Identity=31.2829525483304, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI1789193, Length=569, Percent_Identity=32.8646748681898, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI1787994, Length=399, Percent_Identity=26.3157894736842, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI226510935, Length=195, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 67050; Mature: 67050

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAVVTPAGSWTGRCYDRFMSLQMFGIPVSRGVAIGRAVLVASSRVDVAHYFIEPAQVER
CEEEECCCCCCCHHHHHHHHHHHHCCCCHHCCHHHHHHHEEECCCCHHHHHHCCHHHHHH
EIARLLQARDAVAAELGGLQRDLPEDAPAELSALLDVHLMLLHDEALTGATSQWVHERHY
HHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
NAEWALSAQLEVLARHFDDMENDYLRERKADLEQVVERLLRVLMHDSSAVPPSIGVNPRD
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
FAGEDPLVLVANDIAPADMLQFKRSVFTGFVTDVGGKTSHTAIVARSLDIPAVVGAREAS
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCHHHH
RIIRQDDWVVIDGDAGVVIVDPSSIVLEEYRFRQRQSELERVRLTRLRHTPAVTLDGERV
HHHCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
ELFANIELPGDAAAALEAGAVGVGLFRSEFLFMNRTDDLPGEDEQYQAYCAVVDAMKGLP
EEEEEEECCCCHHHHHHCCCHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
VTIRTVDIGADKPLDRMSAHELRHEHALNPALGLRAIRWSLSEPSMFRQQLRAILRASAH
EEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
GQVRLLVPMLAHESEIRGTFDALARAKQQLTESGRAFGDVQVGAMIEVPAAALMIDRFLD
CCEEEEEHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECEEEECHHHHHHHHHHHH
AFDFVSLGTNDLIQYTLAIDRADEAVAHLYDPWHPAVLELVARTIRAARARGRAVSVCGE
HHHHHHCCCHHHEEEHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
MAGDPSFTSVLLAMGLRSFSMHPSQIAAIKQQILRTDTRRLSDLLLGARSDAPTFTPLRN
HCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
GGGVAATPPRP
CCCCCCCCCCC
>Mature Secondary Structure
MCAVVTPAGSWTGRCYDRFMSLQMFGIPVSRGVAIGRAVLVASSRVDVAHYFIEPAQVER
CEEEECCCCCCCHHHHHHHHHHHHCCCCHHCCHHHHHHHEEECCCCHHHHHHCCHHHHHH
EIARLLQARDAVAAELGGLQRDLPEDAPAELSALLDVHLMLLHDEALTGATSQWVHERHY
HHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
NAEWALSAQLEVLARHFDDMENDYLRERKADLEQVVERLLRVLMHDSSAVPPSIGVNPRD
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
FAGEDPLVLVANDIAPADMLQFKRSVFTGFVTDVGGKTSHTAIVARSLDIPAVVGAREAS
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCHHHH
RIIRQDDWVVIDGDAGVVIVDPSSIVLEEYRFRQRQSELERVRLTRLRHTPAVTLDGERV
HHHCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
ELFANIELPGDAAAALEAGAVGVGLFRSEFLFMNRTDDLPGEDEQYQAYCAVVDAMKGLP
EEEEEEECCCCHHHHHHCCCHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
VTIRTVDIGADKPLDRMSAHELRHEHALNPALGLRAIRWSLSEPSMFRQQLRAILRASAH
EEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
GQVRLLVPMLAHESEIRGTFDALARAKQQLTESGRAFGDVQVGAMIEVPAAALMIDRFLD
CCEEEEEHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECEEEECHHHHHHHHHHHH
AFDFVSLGTNDLIQYTLAIDRADEAVAHLYDPWHPAVLELVARTIRAARARGRAVSVCGE
HHHHHHCCCHHHEEEHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
MAGDPSFTSVLLAMGLRSFSMHPSQIAAIKQQILRTDTRRLSDLLLGARSDAPTFTPLRN
HCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
GGGVAATPPRP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]