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The map label for this gene is lipA [H]

Identifier: 124265509

GI number: 124265509

Start: 341846

End: 342865

Strand: Direct

Name: lipA [H]

Synonym: Mpe_A0316

Alternate gene names: 124265509

Gene position: 341846-342865 (Clockwise)

Preceding gene: 124265508

Following gene: 124265510

Centisome position: 8.45

GC content: 69.22

Gene sequence:

>1020_bases
ATGAACACGCTGCCCTCCACGCCGACCCCGACCTCCGTCTCACCGTCGCCGGCTGCAGGTGAAGCGGCCTACGACCCGCT
CGCCAAGCAGAAGGCGCAAGCCAAGACGGCGCGCATCCCGATCAAGGTGGTGGCGGCCGAGACGCTGAAGAAGCCCGACT
GGATCCGCGTCAAGGCCGGCAGCCCCAGCACCCGCTTCTACGAGATCAAGCAGATCCTGCGCGAGCACAAGCTCCACACC
GTCTGCGAAGAGGCCAGCTGCCCGAACATCGGCGAGTGCTTCGGCAAGGGCACCGCCACCTTCATGATCATGGGCGACAA
GTGCACCCGGCGCTGCCCGTTCTGCGACGTCGGCCACGGCCGCCCCGACCCGCTCGACCCCGACGAACCGCAGAACCTGG
CCAAGACCATCGCCGCGCTCAAGCTCAGCTACGTGGTCATCACCAGCGTCGACCGCGACGACCTGCGCGACGGCGGCGCG
GCCCACTTCGTCGAGTGCATCCGCCAGGTGCGGGCCCTCTCCCCGGCCACCCGCATCGAGATCCTCACGCCCGACTTCCG
CGGCCGCGCCGACCGCGCGCTGGAGATCTTGAAGGCGGCCCCGCCGGACGTGATGAACCACAACCTCGAGACCGCCCCGC
GCCTGTACAAGGAAGCGCGGCCCGGCTCGGACTACCAGTTCAGCCTGGACCTGCTCAAGCGCTTCAAGGCCTTCGCCCCG
GGCGTGCCGACCAAGAGCGGGATCATGGTCGGGCTGGGCGAGACGGACGAGGAGATCCTGCAGGTCATGCGCGACATGCG
TGCGCACGACATCGACATGATCACCATCGGGCAGTACCTGGCGCCCAGCGGGCACCACCTGCCGGTGCGGCGCTACGTGA
CGCCCGAGACCTTCAAGCGCTTCGAGGCCGAGGCCTACGCGATGGGCTTCAGCCACGCCGCCGTGGGCGCCATGGTGCGC
TCGAGCTACCACGCCGACCAGCAGGCCCACGCCGCGGGTGTGGGTGTCGTCGCAGGCTGA

Upstream 100 bases:

>100_bases
TCTACAATCGGGGTCCCCGTCGATCCGGAACGCGCCGCCGCCGACCTCGGTGACCGGCTCGAGAGCTATCTCAGCCCCTG
ACACCGCGCGCCCACATTCC

Downstream 100 bases:

>100_bases
GACGCCGCGGCCGATGATCTGGCCGCCGTTGCGGGCCTGGCTGCCGGCGCGCCTCCCGGTCGGGCCGACCGAGCGAGCGC
GGGCCGCGCTGGGCGCGGCG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNTLPSTPTPTSVSPSPAAGEAAYDPLAKQKAQAKTARIPIKVVAAETLKKPDWIRVKAGSPSTRFYEIKQILREHKLHT
VCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPDEPQNLAKTIAALKLSYVVITSVDRDDLRDGGA
AHFVECIRQVRALSPATRIEILTPDFRGRADRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLDLLKRFKAFAP
GVPTKSGIMVGLGETDEEILQVMRDMRAHDIDMITIGQYLAPSGHHLPVRRYVTPETFKRFEAEAYAMGFSHAAVGAMVR
SSYHADQQAHAAGVGVVAG

Sequences:

>Translated_339_residues
MNTLPSTPTPTSVSPSPAAGEAAYDPLAKQKAQAKTARIPIKVVAAETLKKPDWIRVKAGSPSTRFYEIKQILREHKLHT
VCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPDEPQNLAKTIAALKLSYVVITSVDRDDLRDGGA
AHFVECIRQVRALSPATRIEILTPDFRGRADRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLDLLKRFKAFAP
GVPTKSGIMVGLGETDEEILQVMRDMRAHDIDMITIGQYLAPSGHHLPVRRYVTPETFKRFEAEAYAMGFSHAAVGAMVR
SSYHADQQAHAAGVGVVAG
>Mature_339_residues
MNTLPSTPTPTSVSPSPAAGEAAYDPLAKQKAQAKTARIPIKVVAAETLKKPDWIRVKAGSPSTRFYEIKQILREHKLHT
VCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPDEPQNLAKTIAALKLSYVVITSVDRDDLRDGGA
AHFVECIRQVRALSPATRIEILTPDFRGRADRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLDLLKRFKAFAP
GVPTKSGIMVGLGETDEEILQVMRDMRAHDIDMITIGQYLAPSGHHLPVRRYVTPETFKRFEAEAYAMGFSHAAVGAMVR
SSYHADQQAHAAGVGVVAG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=295, Percent_Identity=45.7627118644068, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI37577164, Length=257, Percent_Identity=45.9143968871595, Blast_Score=221, Evalue=9e-58,
Organism=Escherichia coli, GI1786846, Length=309, Percent_Identity=61.8122977346278, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32564533, Length=256, Percent_Identity=45.3125, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=319, Percent_Identity=42.6332288401254, Blast_Score=250, Evalue=2e-67,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=46.6898954703833, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 37063; Mature: 37063

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLPSTPTPTSVSPSPAAGEAAYDPLAKQKAQAKTARIPIKVVAAETLKKPDWIRVKAG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCEEEEECC
SPSTRFYEIKQILREHKLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCC
RPDPLDPDEPQNLAKTIAALKLSYVVITSVDRDDLRDGGAAHFVECIRQVRALSPATRIE
CCCCCCCCCHHHHHHHHHHHHHEEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEE
ILTPDFRGRADRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLDLLKRFKAFAP
EECCCCCCCHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCC
GVPTKSGIMVGLGETDEEILQVMRDMRAHDIDMITIGQYLAPSGHHLPVRRYVTPETFKR
CCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHCCCCCCCCEEECCCHHHHHH
FEAEAYAMGFSHAAVGAMVRSSYHADQQAHAAGVGVVAG
HHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MNTLPSTPTPTSVSPSPAAGEAAYDPLAKQKAQAKTARIPIKVVAAETLKKPDWIRVKAG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCEEEEECC
SPSTRFYEIKQILREHKLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCC
RPDPLDPDEPQNLAKTIAALKLSYVVITSVDRDDLRDGGAAHFVECIRQVRALSPATRIE
CCCCCCCCCHHHHHHHHHHHHHEEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEE
ILTPDFRGRADRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLDLLKRFKAFAP
EECCCCCCCHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCC
GVPTKSGIMVGLGETDEEILQVMRDMRAHDIDMITIGQYLAPSGHHLPVRRYVTPETFKR
CCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHCCCCCCCCEEECCCHHHHHH
FEAEAYAMGFSHAAVGAMVRSSYHADQQAHAAGVGVVAG
HHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA