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The map label for this gene is lipB

Identifier: 124265508

GI number: 124265508

Start: 341152

End: 341826

Strand: Direct

Name: lipB

Synonym: Mpe_A0315

Alternate gene names: 124265508

Gene position: 341152-341826 (Clockwise)

Preceding gene: 124265507

Following gene: 124265509

Centisome position: 8.44

GC content: 68.74

Gene sequence:

>675_bases
ATGGCGATGCAGCGACGGCGGCTGGGCCGCGTGGACTATGCGACGACCTTCGACGCGATGCGCGCCTTCACCGAGACGCG
CACGCCGGGCACGCCCGATGAGCTGTGGCTGTGCGAGCACCCGCCGGTGTTCACCCAGGGACTGGCCGGCAAGGCCGAGC
ACGTGCTCGACGCGGGAGACATTCCGGTGGTCGCCAGCAACCGCGGCGGCCAGGTGACCTATCACGGCCCCGGCCAGGTG
GTGGCCTATCCGCTGATCGATCTCCAGGCGCTGGGCATCTTCGTCAAGGAGTATGTGTTCCGCCTCGAGCAGGCGGTGAT
CAAGACGCTGGAAGGCTATGGCGTCACCGGCCACCGGGTCACGGGCGCGCCGGGCATCTACGTGAATCTCTGCGACCCCT
TCGGCCACGGCGCGCTGCCGGGGCCGCCCGACCCGCGTGACCCCTGGCGTGGCATCGGCAAGATCGCCGCGCTGGGGATC
AAGGTGTCGCGCCACTGCAGCTACCACGGTCTGGCGTTGAACGTGGCGATGGATCTGAGCCCCTATGAGCGCATCAATCC
CTGCGGCTACGCCGGGCTGCGCACGGTCGATCTTTCTACAATCGGGGTCCCCGTCGATCCGGAACGCGCCGCCGCCGACC
TCGGTGACCGGCTCGAGAGCTATCTCAGCCCCTGA

Upstream 100 bases:

>100_bases
GTGCCGAAGGCGGCGGATGGCTGGGCGCCGGGATCGCCGCGCTGCAGGCGGCTGCAGCGGTGTTCGTCGCGCGCACCCGC
TGGCGGCCGGCGGGGGCCTG

Downstream 100 bases:

>100_bases
CACCGCGCGCCCACATTCCATGAACACGCTGCCCTCCACGCCGACCCCGACCTCCGTCTCACCGTCGCCGGCTGCAGGTG
AAGCGGCCTACGACCCGCTC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MAMQRRRLGRVDYATTFDAMRAFTETRTPGTPDELWLCEHPPVFTQGLAGKAEHVLDAGDIPVVASNRGGQVTYHGPGQV
VAYPLIDLQALGIFVKEYVFRLEQAVIKTLEGYGVTGHRVTGAPGIYVNLCDPFGHGALPGPPDPRDPWRGIGKIAALGI
KVSRHCSYHGLALNVAMDLSPYERINPCGYAGLRTVDLSTIGVPVDPERAAADLGDRLESYLSP

Sequences:

>Translated_224_residues
MAMQRRRLGRVDYATTFDAMRAFTETRTPGTPDELWLCEHPPVFTQGLAGKAEHVLDAGDIPVVASNRGGQVTYHGPGQV
VAYPLIDLQALGIFVKEYVFRLEQAVIKTLEGYGVTGHRVTGAPGIYVNLCDPFGHGALPGPPDPRDPWRGIGKIAALGI
KVSRHCSYHGLALNVAMDLSPYERINPCGYAGLRTVDLSTIGVPVDPERAAADLGDRLESYLSP
>Mature_223_residues
AMQRRRLGRVDYATTFDAMRAFTETRTPGTPDELWLCEHPPVFTQGLAGKAEHVLDAGDIPVVASNRGGQVTYHGPGQVV
AYPLIDLQALGIFVKEYVFRLEQAVIKTLEGYGVTGHRVTGAPGIYVNLCDPFGHGALPGPPDPRDPWRGIGKIAALGIK
VSRHCSYHGLALNVAMDLSPYERINPCGYAGLRTVDLSTIGVPVDPERAAADLGDRLESYLSP

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=212, Percent_Identity=31.6037735849057, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI87081767, Length=213, Percent_Identity=47.887323943662, Blast_Score=188, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323268, Length=140, Percent_Identity=33.5714285714286, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355483, Length=203, Percent_Identity=30.5418719211823, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_METPP (A2SCI8)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019512.1
- ProteinModelPortal:   A2SCI8
- SMR:   A2SCI8
- STRING:   A2SCI8
- GeneID:   4786865
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0315
- NMPDR:   fig|279263.3.peg.138
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   A2SCI8
- ProtClustDB:   PRK14346
- BioCyc:   MPET420662:MPE_A0315-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24207; Mature: 24076

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMQRRRLGRVDYATTFDAMRAFTETRTPGTPDELWLCEHPPVFTQGLAGKAEHVLDAGD
CCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHCCCCCHHHHCCCCC
IPVVASNRGGQVTYHGPGQVVAYPLIDLQALGIFVKEYVFRLEQAVIKTLEGYGVTGHRV
CCEEECCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TGAPGIYVNLCDPFGHGALPGPPDPRDPWRGIGKIAALGIKVSRHCSYHGLALNVAMDLS
CCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEHCCCCCCCCEEEEEEECCC
PYERINPCGYAGLRTVDLSTIGVPVDPERAAADLGDRLESYLSP
CHHHCCCCCCCCEEEEEHEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AMQRRRLGRVDYATTFDAMRAFTETRTPGTPDELWLCEHPPVFTQGLAGKAEHVLDAGD
CCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHCCCCCHHHHCCCCC
IPVVASNRGGQVTYHGPGQVVAYPLIDLQALGIFVKEYVFRLEQAVIKTLEGYGVTGHRV
CCEEECCCCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TGAPGIYVNLCDPFGHGALPGPPDPRDPWRGIGKIAALGIKVSRHCSYHGLALNVAMDLS
CCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEHCCCCCCCCEEEEEEECCC
PYERINPCGYAGLRTVDLSTIGVPVDPERAAADLGDRLESYLSP
CHHHCCCCCCCCEEEEEHEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA