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The map label for this gene is dat [H]

Identifier: 124265505

GI number: 124265505

Start: 337608

End: 338519

Strand: Direct

Name: dat [H]

Synonym: Mpe_A0312

Alternate gene names: 124265505

Gene position: 337608-338519 (Clockwise)

Preceding gene: 124265504

Following gene: 124265506

Centisome position: 8.35

GC content: 69.96

Gene sequence:

>912_bases
ATGAACCCGCTGCCCAGCGCCATCCCCGCGGCGAGCGCCGACTCGCTGTGCTACCTGAACGGCGACTACACCCGCCTGGC
GGACGCTCGCGTCAGCGTGCTCGACCGCGGCTTCATGTTCGGCGACGGCGTCTACGAGGTCCTGCCCGTCTACGATCGTC
GGCTGTTCCGCTTCGACGAGCACATGGCGCGCCTGGAGCGCAGCCTCGCCAAGGTGCGCATCACCGCGCCGCTGACCCGC
GAGGACTGGCTGGCGCGCATGCGCCGGCTGGTCGCGGCCCAGCACGAGCACAGCGGGGCGACCGACCAGCTCGTGTACCT
GCAGGTCACGCGCGGCGTGGCGCTGCGCGAGCACACGATGCCGACCGACATCGAGCCCACGGTCTTCATGATGTGCAGTC
CGGCGAAGCCGCCGACGCCCGAGCAGCGCCATGCCGGCGTGGCCTGCATCAGCGCGCGCGACTTCCGCTGGGAGCGCGGC
GACATCAAGAGCATTTCGCTGCTCGGCAACGTGCTGGCGCGGCAGATGTCGGCCGACAAGGGCGCCGTCGAGACCCTCCT
GTTTCGCGACGGCTTCCTGACCGAGGCAGCGGCGTCCAACGTGTGGATGGTGAAGGAAGGCGCACTGATCGGCCCGCCGA
AGAGCGAACTGCTGCTCGAAGGCGTGCGGGTCGACCTGCTGGCCGAGCTGTGCGAGGAGTGCGGCATCGGCTACAGCCTG
CGGCCGGTCAGCGAGGGCGAGGTCTTCTCGGCCGACGAACTGCTGCTGAGTTCGGCGATGAAGGAAGTGCTGGCGGTCAC
CCGTCTCGATGGCGAACTGGTCGGGCACGGCGCGTTGCGCGGCAAGCCCGGGCCGGTGTACGCCCGGCTCTACGAGGCCT
ACCAGCGGGCCAAGCCCGCCCAGTCGATCTGA

Upstream 100 bases:

>100_bases
GTGGGATTCGCTGCGGCTGTGGATCCAGTGATGCCGGTGTCGATTCGTACCGGGTGAAACGCTGCTTGCCCGTTGCCGCC
GACGGGCCTACGCTGCGGGC

Downstream 100 bases:

>100_bases
GCCGCGGCGGCCGCGCGGATTTCGCGGGTGCGGGACAATCCGGCCATCGCGGGCGCCGCAGCGGCGTGCCCGCCCGATGC
AGCGGCAGCAGACCATGACG

Product: branched subunit Amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNPLPSAIPAASADSLCYLNGDYTRLADARVSVLDRGFMFGDGVYEVLPVYDRRLFRFDEHMARLERSLAKVRITAPLTR
EDWLARMRRLVAAQHEHSGATDQLVYLQVTRGVALREHTMPTDIEPTVFMMCSPAKPPTPEQRHAGVACISARDFRWERG
DIKSISLLGNVLARQMSADKGAVETLLFRDGFLTEAAASNVWMVKEGALIGPPKSELLLEGVRVDLLAELCEECGIGYSL
RPVSEGEVFSADELLLSSAMKEVLAVTRLDGELVGHGALRGKPGPVYARLYEAYQRAKPAQSI

Sequences:

>Translated_303_residues
MNPLPSAIPAASADSLCYLNGDYTRLADARVSVLDRGFMFGDGVYEVLPVYDRRLFRFDEHMARLERSLAKVRITAPLTR
EDWLARMRRLVAAQHEHSGATDQLVYLQVTRGVALREHTMPTDIEPTVFMMCSPAKPPTPEQRHAGVACISARDFRWERG
DIKSISLLGNVLARQMSADKGAVETLLFRDGFLTEAAASNVWMVKEGALIGPPKSELLLEGVRVDLLAELCEECGIGYSL
RPVSEGEVFSADELLLSSAMKEVLAVTRLDGELVGHGALRGKPGPVYARLYEAYQRAKPAQSI
>Mature_303_residues
MNPLPSAIPAASADSLCYLNGDYTRLADARVSVLDRGFMFGDGVYEVLPVYDRRLFRFDEHMARLERSLAKVRITAPLTR
EDWLARMRRLVAAQHEHSGATDQLVYLQVTRGVALREHTMPTDIEPTVFMMCSPAKPPTPEQRHAGVACISARDFRWERG
DIKSISLLGNVLARQMSADKGAVETLLFRDGFLTEAAASNVWMVKEGALIGPPKSELLLEGVRVDLLAELCEECGIGYSL
RPVSEGEVFSADELLLSSAMKEVLAVTRLDGELVGHGALRGKPGPVYARLYEAYQRAKPAQSI

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=294, Percent_Identity=26.530612244898, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 33446; Mature: 33446

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLPSAIPAASADSLCYLNGDYTRLADARVSVLDRGFMFGDGVYEVLPVYDRRLFRFDE
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
HMARLERSLAKVRITAPLTREDWLARMRRLVAAQHEHSGATDQLVYLQVTRGVALREHTM
HHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHCCC
PTDIEPTVFMMCSPAKPPTPEQRHAGVACISARDFRWERGDIKSISLLGNVLARQMSADK
CCCCCCEEEEEECCCCCCCCCHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GAVETLLFRDGFLTEAAASNVWMVKEGALIGPPKSELLLEGVRVDLLAELCEECGIGYSL
HHHHHHHHCCCCHHHHHCCCEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
RPVSEGEVFSADELLLSSAMKEVLAVTRLDGELVGHGALRGKPGPVYARLYEAYQRAKPA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHHHHCCCC
QSI
CCC
>Mature Secondary Structure
MNPLPSAIPAASADSLCYLNGDYTRLADARVSVLDRGFMFGDGVYEVLPVYDRRLFRFDE
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
HMARLERSLAKVRITAPLTREDWLARMRRLVAAQHEHSGATDQLVYLQVTRGVALREHTM
HHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHCCC
PTDIEPTVFMMCSPAKPPTPEQRHAGVACISARDFRWERGDIKSISLLGNVLARQMSADK
CCCCCCEEEEEECCCCCCCCCHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GAVETLLFRDGFLTEAAASNVWMVKEGALIGPPKSELLLEGVRVDLLAELCEECGIGYSL
HHHHHHHHCCCCHHHHHCCCEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
RPVSEGEVFSADELLLSSAMKEVLAVTRLDGELVGHGALRGKPGPVYARLYEAYQRAKPA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHHHHCCCC
QSI
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]