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The map label for this gene is def1 [H]

Identifier: 124265476

GI number: 124265476

Start: 308903

End: 309415

Strand: Direct

Name: def1 [H]

Synonym: Mpe_A0283

Alternate gene names: 124265476

Gene position: 308903-309415 (Clockwise)

Preceding gene: 124265472

Following gene: 124265477

Centisome position: 7.64

GC content: 63.16

Gene sequence:

>513_bases
ATGCCGTTACTCGACATCCTGCGTTATCCCGATCCCCGCCTTCACACCGTGGCCAAACCGGTAAAGGAAGTCGACGAGCG
CGTGCGCCAACTTGTCGACGACATGCTGGAGACCATGTACGACGCCCAGGGCGTCGGACTGGCGGCGACCCAGGTCGACG
TGCACGAGCGCGTGATCGTCATCGACGTTTCCGACGATCGCAACGAGCCCCGGGTGCTGATCAACCCCGAGTTGCTGCAG
GTCAGCGACGAGATGGTCGTGGGGGACGAGGGCTGCCTGTCGGTGCCGCAGATCTACGACAAGGTCCCTCGCCACGCGAA
GGTCACGGTCCGGGCGTTGGGGCGGGACGGGCAGGCCTACCAGTTCGATGCCGAGGAACTGCTGTCGGTGTGCGTTCAGC
ACGAGATGGATCACCTGATGGGCAAGGTCTTCGTCGAGTACCTGTCCCCGCTCAAGCGCAACCGCATCAAGAGCAAGATG
CTCAAGAAAACGCGCGACGAGGCGCGAGACTGA

Upstream 100 bases:

>100_bases
CAATGAAACCGCCGGGCGCGCGGGTGCAGCCATCGGTAGCCCTTCACGCCCGCTCGTTGCTTGTGGCCCACACCGTCCCG
AAGCATTTCGTTACTCTGCC

Downstream 100 bases:

>100_bases
GGCGGACTGCGTGCGCATCGCATTTGCCGGCACGCCCGAGTTCGCGCGTGTTGCGCTGAGGCAGTTGCTGGACGCCGGGT
TCGACGTTCCGCTGGTGCTG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MPLLDILRYPDPRLHTVAKPVKEVDERVRQLVDDMLETMYDAQGVGLAATQVDVHERVIVIDVSDDRNEPRVLINPELLQ
VSDEMVVGDEGCLSVPQIYDKVPRHAKVTVRALGRDGQAYQFDAEELLSVCVQHEMDHLMGKVFVEYLSPLKRNRIKSKM
LKKTRDEARD

Sequences:

>Translated_170_residues
MPLLDILRYPDPRLHTVAKPVKEVDERVRQLVDDMLETMYDAQGVGLAATQVDVHERVIVIDVSDDRNEPRVLINPELLQ
VSDEMVVGDEGCLSVPQIYDKVPRHAKVTVRALGRDGQAYQFDAEELLSVCVQHEMDHLMGKVFVEYLSPLKRNRIKSKM
LKKTRDEARD
>Mature_169_residues
PLLDILRYPDPRLHTVAKPVKEVDERVRQLVDDMLETMYDAQGVGLAATQVDVHERVIVIDVSDDRNEPRVLINPELLQV
SDEMVVGDEGCLSVPQIYDKVPRHAKVTVRALGRDGQAYQFDAEELLSVCVQHEMDHLMGKVFVEYLSPLKRNRIKSKML
KKTRDEARD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=164, Percent_Identity=28.0487804878049, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=56.2130177514793, Blast_Score=196, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24645728, Length=164, Percent_Identity=29.2682926829268, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19462; Mature: 19330

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLDILRYPDPRLHTVAKPVKEVDERVRQLVDDMLETMYDAQGVGLAATQVDVHERVIV
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHEEEHHHCEEEE
IDVSDDRNEPRVLINPELLQVSDEMVVGDEGCLSVPQIYDKVPRHAKVTVRALGRDGQAY
EEECCCCCCCEEEECHHHHHHCCCEEECCCCCCCHHHHHHHCCCCHHHHHEEECCCCCEE
QFDAEELLSVCVQHEMDHLMGKVFVEYLSPLKRNRIKSKMLKKTRDEARD
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLLDILRYPDPRLHTVAKPVKEVDERVRQLVDDMLETMYDAQGVGLAATQVDVHERVIV
CHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHEEEHHHCEEEE
IDVSDDRNEPRVLINPELLQVSDEMVVGDEGCLSVPQIYDKVPRHAKVTVRALGRDGQAY
EEECCCCCCCEEEECHHHHHHCCCEEECCCCCCCHHHHHHHCCCCHHHHHEEECCCCCEE
QFDAEELLSVCVQHEMDHLMGKVFVEYLSPLKRNRIKSKMLKKTRDEARD
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]