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The map label for this gene is amtB [H]

Identifier: 124265365

GI number: 124265365

Start: 185012

End: 186538

Strand: Direct

Name: amtB [H]

Synonym: Mpe_A0172

Alternate gene names: 124265365

Gene position: 185012-186538 (Clockwise)

Preceding gene: 124265364

Following gene: 124265366

Centisome position: 4.57

GC content: 67.32

Gene sequence:

>1527_bases
ATGAGAAAGCTCATTGCCTCCCTCGCCACGTCGTTGGCGCTCGGCGTTGCGGTACTCGGTTTCGCCGATGTCGCGATGGC
CCAGGAAGCTGCATCGGCGCCCGCCGCTGCCGCAGTCGTCGAAGCGGCCGCCGCCGCTACCCCTGCTGCCGAAGCCTCCG
CGCCCGAAGCGGCGCCGGCCGCAGCCGCGCCCGTGCCCAACAAGGGCGACGTCGCGTGGATGCTGGTGTCCACGCTGCTG
GTCATCATGATGTCGATCCCCGGCCTGGCGCTGTTCTACGGCGGCCTGGTGCGCAGCAAGAACATGCTGTCGGTGCTGAT
GCAGGTCTTCGTGGTGTTCTCGCTGATCAGCGTGCTGTGGTTCATCTACGGCTACAGCCTGGCGTTCACCGAAGGCAACG
CCTACATCGGCGGCTTCGACCGGCTGTTCCTGAAGGGCGTGTTCGATCCCGCCACCGGCGCATTCGCGATGGGTGCCACC
TTCAGCAAGGGCGTGGTCATCCCTGAGATCGTGTTCGTCGCCTTCCAGGCCACGTTCGCGGCGATCACCTGCGGCCTGAT
CGTCGGCGCCTTCGCCGAGCGCATCAAGTTCAGCGCGGTGCTGCTGTTCGTCACGCTGTGGTTCACCTTCAGCTACACGC
CGATCGCCCACATGGTGTGGTACTGGATGGGTCCGGACGCCTACACCGGCAAGGAAGTGGTTGATGCCATGAATGGCAAG
GCCGGCCAGATCTGGCAGTGGGGTGCGCTGGACTTCGCTGGCGGCACTGTGGTGCACATCAACGCCGCCGTCGCGGGTCT
GGTGGGTGCCTACATGGTCGGCAAGCGCATCGGCTACGGCAAGGAAGCCTTCACGCCGCACTCGCTGACGCTGACGATGG
TCGGTGCCTCGCTGCTGTGGGTGGGCTGGTTCGGCTTCAACGCGGGCTCCGCGCTGGAAGCCGGCAACAGCGCCGCGCTG
GCCTTCCTGAACACCTTCTCGGCCACGGCCTGTGCCGTGCTGGCCTGGTGCGTCGGTGAGTCGCTGTTCAAGGGCAAGGC
CTCGATGCTGGGTGCGGCGTCGGGTGCGGTGGCCGGCCTGGTGGCCATCACGCCGGCCGCCGGCAACGTCGGCATCGTCG
GCGCCATGGTGATCGGCCTCGTCGCCGGCTTCGCCTGCCTGTGGGGTGTCAGCGGCCTGAAGAAGCTGCTCGGCGCGGAC
GATTCGCTCGACGTGTTCGGCGTCCATGGCGTCGGCGGCATCGTCGGTGCGCTGCTGACCGGCGTGTTCAACTCGCCGGC
CCTGGGCGGCCCGGGCTACGTGACCGACTGGGTCACCGCCAGCGTCATCTCGGCGGCCGACTACTCGATCGCCTCGCAGG
TGTGGATCCAGGCCAAGGCCGTGCTGCTGACCATCGTCTGGTCGGGCGTGGTCTCGGTGGTGGCCTACAAGATCACCGAC
CTGGTGATCGGCCTGCGCGTTAGCGAGGATGCCGAGCGCGAAGGCCTCGACATCACCTCGCACGGCGAGACCGCGTACCA
CAAGTGA

Upstream 100 bases:

>100_bases
GCAAGGTCTTCGTCACCAACCTCGAGCAGGTGATCCGCATCCGCACCGGCGAAACCGGCAAGGACGCGCTCTGAGCGCTT
TCAACAGGAACGAGTCAAAC

Downstream 100 bases:

>100_bases
TCTCTCCGTAAGTTGCCCCGTGCCGGCCCACGCCGGCCGGGGCTTGCAGCCAGGCCGCCTTCGGGCGGCCTTTTTTATGG
CCGGACTTGCAAGCAGCGCG

Product: putative ammonium transporter transmembrane protein

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MRKLIASLATSLALGVAVLGFADVAMAQEAASAPAAAAVVEAAAAATPAAEASAPEAAPAAAAPVPNKGDVAWMLVSTLL
VIMMSIPGLALFYGGLVRSKNMLSVLMQVFVVFSLISVLWFIYGYSLAFTEGNAYIGGFDRLFLKGVFDPATGAFAMGAT
FSKGVVIPEIVFVAFQATFAAITCGLIVGAFAERIKFSAVLLFVTLWFTFSYTPIAHMVWYWMGPDAYTGKEVVDAMNGK
AGQIWQWGALDFAGGTVVHINAAVAGLVGAYMVGKRIGYGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSALEAGNSAAL
AFLNTFSATACAVLAWCVGESLFKGKASMLGAASGAVAGLVAITPAAGNVGIVGAMVIGLVAGFACLWGVSGLKKLLGAD
DSLDVFGVHGVGGIVGALLTGVFNSPALGGPGYVTDWVTASVISAADYSIASQVWIQAKAVLLTIVWSGVVSVVAYKITD
LVIGLRVSEDAEREGLDITSHGETAYHK

Sequences:

>Translated_508_residues
MRKLIASLATSLALGVAVLGFADVAMAQEAASAPAAAAVVEAAAAATPAAEASAPEAAPAAAAPVPNKGDVAWMLVSTLL
VIMMSIPGLALFYGGLVRSKNMLSVLMQVFVVFSLISVLWFIYGYSLAFTEGNAYIGGFDRLFLKGVFDPATGAFAMGAT
FSKGVVIPEIVFVAFQATFAAITCGLIVGAFAERIKFSAVLLFVTLWFTFSYTPIAHMVWYWMGPDAYTGKEVVDAMNGK
AGQIWQWGALDFAGGTVVHINAAVAGLVGAYMVGKRIGYGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSALEAGNSAAL
AFLNTFSATACAVLAWCVGESLFKGKASMLGAASGAVAGLVAITPAAGNVGIVGAMVIGLVAGFACLWGVSGLKKLLGAD
DSLDVFGVHGVGGIVGALLTGVFNSPALGGPGYVTDWVTASVISAADYSIASQVWIQAKAVLLTIVWSGVVSVVAYKITD
LVIGLRVSEDAEREGLDITSHGETAYHK
>Mature_508_residues
MRKLIASLATSLALGVAVLGFADVAMAQEAASAPAAAAVVEAAAAATPAAEASAPEAAPAAAAPVPNKGDVAWMLVSTLL
VIMMSIPGLALFYGGLVRSKNMLSVLMQVFVVFSLISVLWFIYGYSLAFTEGNAYIGGFDRLFLKGVFDPATGAFAMGAT
FSKGVVIPEIVFVAFQATFAAITCGLIVGAFAERIKFSAVLLFVTLWFTFSYTPIAHMVWYWMGPDAYTGKEVVDAMNGK
AGQIWQWGALDFAGGTVVHINAAVAGLVGAYMVGKRIGYGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSALEAGNSAAL
AFLNTFSATACAVLAWCVGESLFKGKASMLGAASGAVAGLVAITPAAGNVGIVGAMVIGLVAGFACLWGVSGLKKLLGAD
DSLDVFGVHGVGGIVGALLTGVFNSPALGGPGYVTDWVTASVISAADYSIASQVWIQAKAVLLTIVWSGVVSVVAYKITD
LVIGLRVSEDAEREGLDITSHGETAYHK

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=447, Percent_Identity=53.2438478747204, Blast_Score=452, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17550182, Length=469, Percent_Identity=28.997867803838, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17550180, Length=455, Percent_Identity=27.9120879120879, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=451, Percent_Identity=25.0554323725055, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI32564042, Length=454, Percent_Identity=24.4493392070485, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324187, Length=456, Percent_Identity=33.9912280701754, Blast_Score=209, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6325396, Length=450, Percent_Identity=31.5555555555556, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321559, Length=446, Percent_Identity=30.7174887892377, Blast_Score=203, Evalue=5e-53,
Organism=Drosophila melanogaster, GI161078314, Length=448, Percent_Identity=25.2232142857143, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 52178; Mature: 52178

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLIASLATSLALGVAVLGFADVAMAQEAASAPAAAAVVEAAAAATPAAEASAPEAAPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
AAAPVPNKGDVAWMLVSTLLVIMMSIPGLALFYGGLVRSKNMLSVLMQVFVVFSLISVLW
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
FIYGYSLAFTEGNAYIGGFDRLFLKGVFDPATGAFAMGATFSKGVVIPEIVFVAFQATFA
HHHCCEEEEECCCCEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AITCGLIVGAFAERIKFSAVLLFVTLWFTFSYTPIAHMVWYWMGPDAYTGKEVVDAMNGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHCCC
AGQIWQWGALDFAGGTVVHINAAVAGLVGAYMVGKRIGYGKEAFTPHSLTLTMVGASLLW
CCCEEECCCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
VGWFGFNAGSALEAGNSAALAFLNTFSATACAVLAWCVGESLFKGKASMLGAASGAVAGL
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHH
VAITPAAGNVGIVGAMVIGLVAGFACLWGVSGLKKLLGADDSLDVFGVHGVGGIVGALLT
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
GVFNSPALGGPGYVTDWVTASVISAADYSIASQVWIQAKAVLLTIVWSGVVSVVAYKITD
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVIGLRVSEDAEREGLDITSHGETAYHK
HHHEEEECCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKLIASLATSLALGVAVLGFADVAMAQEAASAPAAAAVVEAAAAATPAAEASAPEAAPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
AAAPVPNKGDVAWMLVSTLLVIMMSIPGLALFYGGLVRSKNMLSVLMQVFVVFSLISVLW
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
FIYGYSLAFTEGNAYIGGFDRLFLKGVFDPATGAFAMGATFSKGVVIPEIVFVAFQATFA
HHHCCEEEEECCCCEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AITCGLIVGAFAERIKFSAVLLFVTLWFTFSYTPIAHMVWYWMGPDAYTGKEVVDAMNGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHCCC
AGQIWQWGALDFAGGTVVHINAAVAGLVGAYMVGKRIGYGKEAFTPHSLTLTMVGASLLW
CCCEEECCCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
VGWFGFNAGSALEAGNSAALAFLNTFSATACAVLAWCVGESLFKGKASMLGAASGAVAGL
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHH
VAITPAAGNVGIVGAMVIGLVAGFACLWGVSGLKKLLGADDSLDVFGVHGVGGIVGALLT
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
GVFNSPALGGPGYVTDWVTASVISAADYSIASQVWIQAKAVLLTIVWSGVVSVVAYKITD
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVIGLRVSEDAEREGLDITSHGETAYHK
HHHEEEECCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]