Click here to switch to the map view.

The map label for this gene is 124265366

Identifier: 124265366

GI number: 124265366

Start: 186692

End: 187987

Strand: Direct

Name: 124265366

Synonym: Mpe_A0173

Alternate gene names: NA

Gene position: 186692-187987 (Clockwise)

Preceding gene: 124265365

Following gene: 124265367

Centisome position: 4.62

GC content: 65.51

Gene sequence:

>1296_bases
ATGGTCCCCCACCTCATTACCGCCCTGAACGGACCGATCAACGAGCTGGAGCAGCGCATCCTCGAGTCCATGCCGGCGAT
CGAGCGCTGGTTCCGTCTCGAGTGGATGGAGCACACGCCGCCGTTCTACAGCTCCGTGGACGTGCGCAACGCCGGCTTCA
AGCTGGCGCCGGTCGACACCAACCTCTACCCCGGCGGTTTCAACAACCTCACGCCCGAGATGCTGCCGCTGGCGGTGCAG
GCGGCGATGGCGGCGATCGAGAAGATCTGCCCCGAGGCGAAGAACCTCCTTCTGATCCCCGAGAAGCACACCCGCAACAC
CTTCTACCTGATGAACGTGCAGCGTCTGGTGCAGATCTTCGGCGCTGCCGGCCTGAACGTGCGGCTGGGGACGCTGGACG
ACAGCATCACCGAGCCGACGCGCCTGGCGCTGCCCGACGGCTCCGAACTGACGCTCGAGCCGCTGCTGCGCAGCAAGCGC
CGCCTGGGGCTGAAGGATTTCGACCCCTGCACCATCCTGCTCAACAATGACCTGTCCGCGGGCATCCCGAAGGTGCTCGA
AGGCCTGCACGAGCAATACCTGCTGCCGCCGCTGCATGCCGGCTGGGCGGTGCGGCGCAAGAGCCAGCATTTCCAGGCCT
ACGAGGAAGTGGCCAAGAAGTTCGCCAAGCTGCTGGGCATGGATCCGTGGCTGATCAACCCGATGTTCGCCAAGTGCGGC
AAGGTCAACTTCCAGGACGGCACCGGCCAGGAGTGCGTGCAGAGCCATGCCGACGCGCTGCTCGGCAAGATCCGCCGCAA
GTACAAGGAGTACGGCATCCAGGAGAAGCCGTTCGTCATCGTCAAGGCCGATGCCGGCACCTACGGCATGGGCATCATGA
CGGTGCGCGACGCCAAGGACCTGGACGAGCTCAACCGCAAGGCGCGCAACAAGATGAGCGTCATCAAGGACGGCCAGGAG
GTCTCCGAGGTGATCATCCAGGAGGGCGTGCCGACCTACGAACGCATGAACGACGCCGTGGCCGAGCCGGTGGTCTACAT
GATCGACCGCTACGTGGTCGGCGGCTTCTACCGCGTGCACGCCGAGCGGGGCATCGACGAGAACCTCAATTCGCCGGGGG
CGAGCTTCGTGCCGCTGGCCTTCGCCGAGAGCCACCATCTGCCGCGGCCGGGCGAGAAACCCGGTGCCAGCGCTCCGAAC
CGCTTCTACATGTACGGCGTGATCGGACGGCTGGCGATGCTGGGCGCCAGCTACGAACTCGAAGCGACCGACCCCGACGC
CGAGGTCTACGAGTAG

Upstream 100 bases:

>100_bases
GCCGCCTTCGGGCGGCCTTTTTTATGGCCGGACTTGCAAGCAGCGCGCGAACCGCCACCTGCCTAGAATCCCGTTTCCGG
TTACGCCAAAGGCTTGTTCC

Downstream 100 bases:

>100_bases
CGCGCGCCGGCTTCGCGCCGAGGCGGCATCGCGTTGCTGCGCTGCAACAACCCAAAGCGGATCCGCCCTGGGACGCAGCG
CAGAATGGGGGCCCTCGCCG

Product: putative glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MVPHLITALNGPINELEQRILESMPAIERWFRLEWMEHTPPFYSSVDVRNAGFKLAPVDTNLYPGGFNNLTPEMLPLAVQ
AAMAAIEKICPEAKNLLLIPEKHTRNTFYLMNVQRLVQIFGAAGLNVRLGTLDDSITEPTRLALPDGSELTLEPLLRSKR
RLGLKDFDPCTILLNNDLSAGIPKVLEGLHEQYLLPPLHAGWAVRRKSQHFQAYEEVAKKFAKLLGMDPWLINPMFAKCG
KVNFQDGTGQECVQSHADALLGKIRRKYKEYGIQEKPFVIVKADAGTYGMGIMTVRDAKDLDELNRKARNKMSVIKDGQE
VSEVIIQEGVPTYERMNDAVAEPVVYMIDRYVVGGFYRVHAERGIDENLNSPGASFVPLAFAESHHLPRPGEKPGASAPN
RFYMYGVIGRLAMLGASYELEATDPDAEVYE

Sequences:

>Translated_431_residues
MVPHLITALNGPINELEQRILESMPAIERWFRLEWMEHTPPFYSSVDVRNAGFKLAPVDTNLYPGGFNNLTPEMLPLAVQ
AAMAAIEKICPEAKNLLLIPEKHTRNTFYLMNVQRLVQIFGAAGLNVRLGTLDDSITEPTRLALPDGSELTLEPLLRSKR
RLGLKDFDPCTILLNNDLSAGIPKVLEGLHEQYLLPPLHAGWAVRRKSQHFQAYEEVAKKFAKLLGMDPWLINPMFAKCG
KVNFQDGTGQECVQSHADALLGKIRRKYKEYGIQEKPFVIVKADAGTYGMGIMTVRDAKDLDELNRKARNKMSVIKDGQE
VSEVIIQEGVPTYERMNDAVAEPVVYMIDRYVVGGFYRVHAERGIDENLNSPGASFVPLAFAESHHLPRPGEKPGASAPN
RFYMYGVIGRLAMLGASYELEATDPDAEVYE
>Mature_431_residues
MVPHLITALNGPINELEQRILESMPAIERWFRLEWMEHTPPFYSSVDVRNAGFKLAPVDTNLYPGGFNNLTPEMLPLAVQ
AAMAAIEKICPEAKNLLLIPEKHTRNTFYLMNVQRLVQIFGAAGLNVRLGTLDDSITEPTRLALPDGSELTLEPLLRSKR
RLGLKDFDPCTILLNNDLSAGIPKVLEGLHEQYLLPPLHAGWAVRRKSQHFQAYEEVAKKFAKLLGMDPWLINPMFAKCG
KVNFQDGTGQECVQSHADALLGKIRRKYKEYGIQEKPFVIVKADAGTYGMGIMTVRDAKDLDELNRKARNKMSVIKDGQE
VSEVIIQEGVPTYERMNDAVAEPVVYMIDRYVVGGFYRVHAERGIDENLNSPGASFVPLAFAESHHLPRPGEKPGASAPN
RFYMYGVIGRLAMLGASYELEATDPDAEVYE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 48222; Mature: 48222

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLITALNGPINELEQRILESMPAIERWFRLEWMEHTPPFYSSVDVRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCC
NLYPGGFNNLTPEMLPLAVQAAMAAIEKICPEAKNLLLIPEKHTRNTFYLMNVQRLVQIF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHCCCEEEECCCCCCCEEEEECHHHHHHHH
GAAGLNVRLGTLDDSITEPTRLALPDGSELTLEPLLRSKRRLGLKDFDPCTILLNNDLSA
CCCCCEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCC
GIPKVLEGLHEQYLLPPLHAGWAVRRKSQHFQAYEEVAKKFAKLLGMDPWLINPMFAKCG
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHCC
KVNFQDGTGQECVQSHADALLGKIRRKYKEYGIQEKPFVIVKADAGTYGMGIMTVRDAKD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCHHH
LDELNRKARNKMSVIKDGQEVSEVIIQEGVPTYERMNDAVAEPVVYMIDRYVVGGFYRVH
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
AERGIDENLNSPGASFVPLAFAESHHLPRPGEKPGASAPNRFYMYGVIGRLAMLGASYEL
ECCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCEEE
EATDPDAEVYE
ECCCCCCCCCC
>Mature Secondary Structure
MVPHLITALNGPINELEQRILESMPAIERWFRLEWMEHTPPFYSSVDVRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCC
NLYPGGFNNLTPEMLPLAVQAAMAAIEKICPEAKNLLLIPEKHTRNTFYLMNVQRLVQIF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHCCCEEEECCCCCCCEEEEECHHHHHHHH
GAAGLNVRLGTLDDSITEPTRLALPDGSELTLEPLLRSKRRLGLKDFDPCTILLNNDLSA
CCCCCEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCC
GIPKVLEGLHEQYLLPPLHAGWAVRRKSQHFQAYEEVAKKFAKLLGMDPWLINPMFAKCG
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHCC
KVNFQDGTGQECVQSHADALLGKIRRKYKEYGIQEKPFVIVKADAGTYGMGIMTVRDAKD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCHHH
LDELNRKARNKMSVIKDGQEVSEVIIQEGVPTYERMNDAVAEPVVYMIDRYVVGGFYRVH
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
AERGIDENLNSPGASFVPLAFAESHHLPRPGEKPGASAPNRFYMYGVIGRLAMLGASYEL
ECCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCEEE
EATDPDAEVYE
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA