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The map label for this gene is mls [H]

Identifier: 124265352

GI number: 124265352

Start: 166878

End: 168464

Strand: Direct

Name: mls [H]

Synonym: Mpe_A0159

Alternate gene names: 124265352

Gene position: 166878-168464 (Clockwise)

Preceding gene: 124265351

Following gene: 124265353

Centisome position: 4.13

GC content: 66.41

Gene sequence:

>1587_bases
ATGACGACCCTCCAACTGCCCGCCGGCCTGCAGATCAACGCGGCGATCCTGCCGGGCTTCGAGACCATCCTCACGCCGGC
GGCGCTCGAGCTCGTGGCCAAGCTGCACCGCACCTTCGAGCCCCGCCGCCAGGAACTGCTGGCCGCGCGCGTGGCACGCG
TGAAGCGGCTCGATGCCGGCGAGCGCCCCGATTTCCTGCCGGAGACGAAGGCGATCCGCGAAGGCGACTGGAAGATCGCG
CCGATCCCGAAGGCGCTGGAGTGCCGTCGCGTCGAGATCACCGGGCCGGTCGAGGCCAAGATGGTCATCAACGCCTTCAA
TTCGGGCGCTGATTCGTACATGACCGACTTCGAGGACAGCAACTCGCCGGTCTGGGCGAACCAGATCCAGGGGCAGATCA
ACCTGTACAAGGCGATCCGCCGCACGCTGACGCTGGGCCAGGCGGGCAAGACCTACAAGCTCAACGACAAGATCGCGACG
CTGCAGGTGCGCCCGCGCGGCTGGCACCTCGACGAGAAGCACGTGCTGGTCGACGGCCAGCGCGTCTCCGGCGGCATCTT
CGACTTCGCGCTATTCCTGTTCCACAACGCCAAGGAGCAGATCGAGCGCGGCGCCGGCCCGTTCTTCTACCTGCCGAAGA
TGGAGTCGCACCTCGAGGCGCGGCTGTGGAACGACATCTTCATCGCCGCGCAGAAGGAGATCGGCCTGCCGCAGGGCACG
ATCAAGGCGACGGTGCTGATCGAGACCATCCTCGCCGCGTTCGAGATGGACGAGATCCTGTACGAGCTGCGCGAGCACAG
CGCCGGGCTGAATGCCGGCCGCTGGGACTACATCTTCTCGTGCATCAAGAAGTTCAAGAACGACCGCGACTTCTGCCTGG
CCGACCGCGCCAAGGTCACGATGACGGCGCCCTTCATGCGCGCCTACGCGCTGCTGCTGCTCAAGACCTGCCACAAGCGC
GGCGCGCCCGCGATCGGCGGCATGAGCGCGCTGATCCCGATCAAGAACGACCCCGAGAAGAACGCCATCGCGATGGCCGG
GATCATCAAGGACAAGCGCCGCGACGCGAACGACGGCTACGACGGTGGCTGGGTCGCGCACCCGGGCCTGGTCGAGTCGG
CGATGAAGGAGTTCGTCGACGTGCTGGGCGATCGCCCGAACCAGATCGAGCGCCAGAAGCCCTACACCCACATCACCGCC
GCGCAACTGCTGGAGTTCGCGCCCGAGGCCCCGATCACCGAGGCCGGGCTGCGCATGAACATCAACGTCGGCATCTATTA
CCTGGCGTCCTGGCTGGCCGGCAACGGCTGCGTGCCGATCTACAACCTGATGGAGGACGCCGCCACCGCCGAGATCTCGC
GCTCGCAGGTGTGGCAGTGGATCCGCAGTCCCAAGGGCGTGCTCGCCGACGGCCGCAAGGTCACCGCCGACATGGTGCGC
AAGATGGTGGCCGAGGAGCTCAAGGGCATCAAGGACGCCGGCTACGAAGGCCAGACGGTCGACCGCGCGGCCGAGATCTT
CGAGCAGATGAGCACCCAGGATGCGTTCGCCGAGTTCCTGACGCTGCCGCTGTACGAAGAGATCTGA

Upstream 100 bases:

>100_bases
GAGGCCCTCGACACCGAGCCGACGCGCACCGGCAGCAGCCTGCGCGACGTGCTCGACTTCTTTCCCAAGACCTGAAACTC
CCTGTCCCTAGGAGCTGACC

Downstream 100 bases:

>100_bases
TCGAGGCCGTGAAGGACGGGCTGCGGGGCAGCCCGTCCCCGCCGTGCACCGCGACGCGGCGCGCCGCGGTGCGCACGGTT
CGTGCATGAGTGCGGGTTGA

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MTTLQLPAGLQINAAILPGFETILTPAALELVAKLHRTFEPRRQELLAARVARVKRLDAGERPDFLPETKAIREGDWKIA
PIPKALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDSNSPVWANQIQGQINLYKAIRRTLTLGQAGKTYKLNDKIAT
LQVRPRGWHLDEKHVLVDGQRVSGGIFDFALFLFHNAKEQIERGAGPFFYLPKMESHLEARLWNDIFIAAQKEIGLPQGT
IKATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKNDRDFCLADRAKVTMTAPFMRAYALLLLKTCHKR
GAPAIGGMSALIPIKNDPEKNAIAMAGIIKDKRRDANDGYDGGWVAHPGLVESAMKEFVDVLGDRPNQIERQKPYTHITA
AQLLEFAPEAPITEAGLRMNINVGIYYLASWLAGNGCVPIYNLMEDAATAEISRSQVWQWIRSPKGVLADGRKVTADMVR
KMVAEELKGIKDAGYEGQTVDRAAEIFEQMSTQDAFAEFLTLPLYEEI

Sequences:

>Translated_528_residues
MTTLQLPAGLQINAAILPGFETILTPAALELVAKLHRTFEPRRQELLAARVARVKRLDAGERPDFLPETKAIREGDWKIA
PIPKALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDSNSPVWANQIQGQINLYKAIRRTLTLGQAGKTYKLNDKIAT
LQVRPRGWHLDEKHVLVDGQRVSGGIFDFALFLFHNAKEQIERGAGPFFYLPKMESHLEARLWNDIFIAAQKEIGLPQGT
IKATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKNDRDFCLADRAKVTMTAPFMRAYALLLLKTCHKR
GAPAIGGMSALIPIKNDPEKNAIAMAGIIKDKRRDANDGYDGGWVAHPGLVESAMKEFVDVLGDRPNQIERQKPYTHITA
AQLLEFAPEAPITEAGLRMNINVGIYYLASWLAGNGCVPIYNLMEDAATAEISRSQVWQWIRSPKGVLADGRKVTADMVR
KMVAEELKGIKDAGYEGQTVDRAAEIFEQMSTQDAFAEFLTLPLYEEI
>Mature_527_residues
TTLQLPAGLQINAAILPGFETILTPAALELVAKLHRTFEPRRQELLAARVARVKRLDAGERPDFLPETKAIREGDWKIAP
IPKALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDSNSPVWANQIQGQINLYKAIRRTLTLGQAGKTYKLNDKIATL
QVRPRGWHLDEKHVLVDGQRVSGGIFDFALFLFHNAKEQIERGAGPFFYLPKMESHLEARLWNDIFIAAQKEIGLPQGTI
KATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKNDRDFCLADRAKVTMTAPFMRAYALLLLKTCHKRG
APAIGGMSALIPIKNDPEKNAIAMAGIIKDKRRDANDGYDGGWVAHPGLVESAMKEFVDVLGDRPNQIERQKPYTHITAA
QLLEFAPEAPITEAGLRMNINVGIYYLASWLAGNGCVPIYNLMEDAATAEISRSQVWQWIRSPKGVLADGRKVTADMVRK
MVAEELKGIKDAGYEGQTVDRAAEIFEQMSTQDAFAEFLTLPLYEEI

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=510, Percent_Identity=51.1764705882353, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17561814, Length=524, Percent_Identity=50, Blast_Score=507, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=51.4285714285714, Blast_Score=419, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324212, Length=514, Percent_Identity=49.8054474708171, Blast_Score=503, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6322222, Length=514, Percent_Identity=49.2217898832685, Blast_Score=492, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59006; Mature: 58875

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLQLPAGLQINAAILPGFETILTPAALELVAKLHRTFEPRRQELLAARVARVKRLDAG
CCEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
ERPDFLPETKAIREGDWKIAPIPKALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDS
CCCCCCCCCHHHHCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHCCCCC
NSPVWANQIQGQINLYKAIRRTLTLGQAGKTYKLNDKIATLQVRPRGWHLDEKHVLVDGQ
CCCEEHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCCCCCEEEEECC
RVSGGIFDFALFLFHNAKEQIERGAGPFFYLPKMESHLEARLWNDIFIAAQKEIGLPQGT
CCCCHHHHHHHHHHHCCHHHHHCCCCCEEECCCHHHHHHHHHHHHHEEEEHHHCCCCCCH
IKATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKNDRDFCLADRAKVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEE
MTAPFMRAYALLLLKTCHKRGAPAIGGMSALIPIKNDPEKNAIAMAGIIKDKRRDANDGY
EEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCC
DGGWVAHPGLVESAMKEFVDVLGDRPNQIERQKPYTHITAAQLLEFAPEAPITEAGLRMN
CCCEEECCHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
INVGIYYLASWLAGNGCVPIYNLMEDAATAEISRSQVWQWIRSPKGVLADGRKVTADMVR
ECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
KMVAEELKGIKDAGYEGQTVDRAAEIFEQMSTQDAFAEFLTLPLYEEI
HHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
TTLQLPAGLQINAAILPGFETILTPAALELVAKLHRTFEPRRQELLAARVARVKRLDAG
CEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
ERPDFLPETKAIREGDWKIAPIPKALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDS
CCCCCCCCCHHHHCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHCCCCC
NSPVWANQIQGQINLYKAIRRTLTLGQAGKTYKLNDKIATLQVRPRGWHLDEKHVLVDGQ
CCCEEHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCCCCCEEEEECC
RVSGGIFDFALFLFHNAKEQIERGAGPFFYLPKMESHLEARLWNDIFIAAQKEIGLPQGT
CCCCHHHHHHHHHHHCCHHHHHCCCCCEEECCCHHHHHHHHHHHHHEEEEHHHCCCCCCH
IKATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKNDRDFCLADRAKVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEE
MTAPFMRAYALLLLKTCHKRGAPAIGGMSALIPIKNDPEKNAIAMAGIIKDKRRDANDGY
EEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCC
DGGWVAHPGLVESAMKEFVDVLGDRPNQIERQKPYTHITAAQLLEFAPEAPITEAGLRMN
CCCEEECCHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
INVGIYYLASWLAGNGCVPIYNLMEDAATAEISRSQVWQWIRSPKGVLADGRKVTADMVR
ECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
KMVAEELKGIKDAGYEGQTVDRAAEIFEQMSTQDAFAEFLTLPLYEEI
HHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA