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The map label for this gene is sir [H]

Identifier: 229000868

GI number: 229000868

Start: 726624

End: 728429

Strand: Reverse

Name: sir [H]

Synonym: NATL1_07941

Alternate gene names: 229000868

Gene position: 728429-726624 (Counterclockwise)

Preceding gene: 124025503

Following gene: 124025489

Centisome position: 39.06

GC content: 38.76

Gene sequence:

>1806_bases
ATGAATTTTGATGAAAAGGAAAGTACTAATATTGAAGAGATAGAACAAACTGTTCCATCACCTTGCATAGCTAATAATTC
ACCTCGGGCAAAATTTGAACAATTTAAAGCCGATAGTAATTATTTAAATGAACCCCTACATAGTGAGTTATTAAATGAGA
GCGATCACTTTACAAACGACGCAGTACAACTTTTAAAATTCCACGGTAGTTATCAACAAGACGATCGAGAGCATAGAAAA
AGAGGGGGCACAGGTAAAGATTGGCAAATGATGCTGAGGCTAAGAAATCCAGCTGGTTATGTACCTGGCCCACTCTTTGT
GGCATTAGACGAGCTATCTGATCGACTAGGGAATCAAACCCTTAGAGCGACCACTCGCCAATGCTTCCAAATGCATGGAA
TAAAAAAAAGAAACATTAAAGAAGTAATTGGAACAATAGTCAAATCGATGGGATCTACACTTGCAGCTTGTGGAGACGTC
AACAGAAATGTAATGGCTCCTGCAGCCCCCTACGAACAAGGGTCCTATCCTGCCGCGAGAAAATTAGCTAATGATATTGC
CGATGTTTTATCGCCCAAAAAAGCAGAAAAAACCTACATTGATTTATGGGTAGATGGCGAGATGAAATATGCTATTAAGC
CTTCTTCTGAAGTTAAAAAGAATAGAAAGCTCCAACTAAAGCCTGGTGTTTTTAGTGGGGATAAAAAAGAACCTTTATAT
GGTGCAACCTACTTACCAAGAAAATTCAAATGTGCAACTACAGTCCCTGGAGACAACTCTGTGGACATCCTTACTCATGA
CATAGGTTTGGTGACGTTCACAAACAAAAAAGGAGTTTTAGAAGGATGTAATGTCTATGTGGGAGGAGGCATGGGTAGAA
CACACAACTTAGACACGACATTTGCGAGGATAGCAGACCCAATTGGTTATGTAGAAGGAGAACATATCTTAGAACTTGTT
CAATCTATACTCGCTCTTCAAAGAGATTATGGAGATAGAAAAACAAGAAGACATTCAAGACTAAAATACGTTTTGCATGA
CATGGGTGTGGATTGGTTTAAGAAACAGTTAACATCTAAATATTTTACGAGAAAAATTGAAAACCTAAAGCACGAAGGAG
ATACAATACTCGAAGATTATTTAGGTTGGCATCAACAATCTGAAAAGTTATGGTTTGTTGGTCTACCTTTATTGTCAGGT
AGACTTACAGGAAGGGTAAAGAAAGAGCTTAGAAATATAGTTGAAAAATTCGCATTGGATGTGCGTTTAACTCCCAATCA
AGATCTTTTATTATGCAATATCGGAAATTATCAAAAAGCCAGTGTAAAGAGAGCACTCATTAATATTGGTTTTATGAATC
CAGGGGCTCCTGACCCGTTAGCCAGAAATGCCATGGCTTGTCCAGCTCTTCCATTATGTGGACTGGCAATGACAGAAGCA
GAGAGATTTTTACCTGAGCTCTTGGAGCGAATAAACAATCAATTAGAAATTCTAGAAATCAATAAATCAATCCTAATCAG
AGTAACCGGCTGTCCGAATGGGTGTGCTCGCCCTTATATGGCCGAATTAGCTCTTGTTGGTAGTGGTTTAAATCAATACC
AACTTTGGCTTGGAGGCAGCACTAATTTAAAAAGGTTGGCGACTCCATATCTACAAAAAATGCCTATCGATGACTTAGAG
AAGACATTAGAGCCTTTATTCCTTAGCTGGAAAGATACGGGAGCCTCATCAAGTCTTGGGGATCATGTGACTAAACTTGG
TTCTGAAAGTGTAATGTCTTTACTTACTTCAAGTGCAGCTCCATAA

Upstream 100 bases:

>100_bases
ACTAGAGGCAATTAAAAATAGCAATTCAAAAGAACCCCCAAAAAACATAGAAAAAGACTTAAAAGATGCTGGATTTAGTA
ATGCAGCTATTGAAGCTCTG

Downstream 100 bases:

>100_bases
ATAGGAATACCTTGATTGCTAGTCCAAGCCCATAATTGATCTCCATAGCTAAAATGCCACCACTCGTTTGGGTGTTGAAC
AAATCCAGCCTGTTCCATAA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MNFDEKESTNIEEIEQTVPSPCIANNSPRAKFEQFKADSNYLNEPLHSELLNESDHFTNDAVQLLKFHGSYQQDDREHRK
RGGTGKDWQMMLRLRNPAGYVPGPLFVALDELSDRLGNQTLRATTRQCFQMHGIKKRNIKEVIGTIVKSMGSTLAACGDV
NRNVMAPAAPYEQGSYPAARKLANDIADVLSPKKAEKTYIDLWVDGEMKYAIKPSSEVKKNRKLQLKPGVFSGDKKEPLY
GATYLPRKFKCATTVPGDNSVDILTHDIGLVTFTNKKGVLEGCNVYVGGGMGRTHNLDTTFARIADPIGYVEGEHILELV
QSILALQRDYGDRKTRRHSRLKYVLHDMGVDWFKKQLTSKYFTRKIENLKHEGDTILEDYLGWHQQSEKLWFVGLPLLSG
RLTGRVKKELRNIVEKFALDVRLTPNQDLLLCNIGNYQKASVKRALINIGFMNPGAPDPLARNAMACPALPLCGLAMTEA
ERFLPELLERINNQLEILEINKSILIRVTGCPNGCARPYMAELALVGSGLNQYQLWLGGSTNLKRLATPYLQKMPIDDLE
KTLEPLFLSWKDTGASSSLGDHVTKLGSESVMSLLTSSAAP

Sequences:

>Translated_601_residues
MNFDEKESTNIEEIEQTVPSPCIANNSPRAKFEQFKADSNYLNEPLHSELLNESDHFTNDAVQLLKFHGSYQQDDREHRK
RGGTGKDWQMMLRLRNPAGYVPGPLFVALDELSDRLGNQTLRATTRQCFQMHGIKKRNIKEVIGTIVKSMGSTLAACGDV
NRNVMAPAAPYEQGSYPAARKLANDIADVLSPKKAEKTYIDLWVDGEMKYAIKPSSEVKKNRKLQLKPGVFSGDKKEPLY
GATYLPRKFKCATTVPGDNSVDILTHDIGLVTFTNKKGVLEGCNVYVGGGMGRTHNLDTTFARIADPIGYVEGEHILELV
QSILALQRDYGDRKTRRHSRLKYVLHDMGVDWFKKQLTSKYFTRKIENLKHEGDTILEDYLGWHQQSEKLWFVGLPLLSG
RLTGRVKKELRNIVEKFALDVRLTPNQDLLLCNIGNYQKASVKRALINIGFMNPGAPDPLARNAMACPALPLCGLAMTEA
ERFLPELLERINNQLEILEINKSILIRVTGCPNGCARPYMAELALVGSGLNQYQLWLGGSTNLKRLATPYLQKMPIDDLE
KTLEPLFLSWKDTGASSSLGDHVTKLGSESVMSLLTSSAAP
>Mature_601_residues
MNFDEKESTNIEEIEQTVPSPCIANNSPRAKFEQFKADSNYLNEPLHSELLNESDHFTNDAVQLLKFHGSYQQDDREHRK
RGGTGKDWQMMLRLRNPAGYVPGPLFVALDELSDRLGNQTLRATTRQCFQMHGIKKRNIKEVIGTIVKSMGSTLAACGDV
NRNVMAPAAPYEQGSYPAARKLANDIADVLSPKKAEKTYIDLWVDGEMKYAIKPSSEVKKNRKLQLKPGVFSGDKKEPLY
GATYLPRKFKCATTVPGDNSVDILTHDIGLVTFTNKKGVLEGCNVYVGGGMGRTHNLDTTFARIADPIGYVEGEHILELV
QSILALQRDYGDRKTRRHSRLKYVLHDMGVDWFKKQLTSKYFTRKIENLKHEGDTILEDYLGWHQQSEKLWFVGLPLLSG
RLTGRVKKELRNIVEKFALDVRLTPNQDLLLCNIGNYQKASVKRALINIGFMNPGAPDPLARNAMACPALPLCGLAMTEA
ERFLPELLERINNQLEILEINKSILIRVTGCPNGCARPYMAELALVGSGLNQYQLWLGGSTNLKRLATPYLQKMPIDDLE
KTLEPLFLSWKDTGASSSLGDHVTKLGSESVMSLLTSSAAP

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=568, Percent_Identity=33.2746478873239, Blast_Score=290, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6322597, Length=577, Percent_Identity=36.7417677642981, Blast_Score=330, Evalue=4e-91,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR011787 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 67213; Mature: 67213

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDEKESTNIEEIEQTVPSPCIANNSPRAKFEQFKADSNYLNEPLHSELLNESDHFTND
CCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHCCHHHHHHCCCCCCCHHH
AVQLLKFHGSYQQDDREHRKRGGTGKDWQMMLRLRNPAGYVPGPLFVALDELSDRLGNQT
HHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCHH
LRATTRQCFQMHGIKKRNIKEVIGTIVKSMGSTLAACGDVNRNVMAPAAPYEQGSYPAAR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
KLANDIADVLSPKKAEKTYIDLWVDGEMKYAIKPSSEVKKNRKLQLKPGVFSGDKKEPLY
HHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCHHHHCCCCEEECCCCCCCCCCCCCC
GATYLPRKFKCATTVPGDNSVDILTHDIGLVTFTNKKGVLEGCNVYVGGGMGRTHNLDTT
CCCCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCCCCCCEEECCCCCCCCCCHHH
FARIADPIGYVEGEHILELVQSILALQRDYGDRKTRRHSRLKYVLHDMGVDWFKKQLTSK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YFTRKIENLKHEGDTILEDYLGWHQQSEKLWFVGLPLLSGRLTGRVKKELRNIVEKFALD
HHHHHHHHHHCCCHHHHHHHHCHHCCCCCEEEEECHHHCCHHHHHHHHHHHHHHHHHHEE
VRLTPNQDLLLCNIGNYQKASVKRALINIGFMNPGAPDPLARNAMACPALPLCGLAMTEA
EEECCCCCEEEEECCCCHHHHHHHHHHHEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHH
ERFLPELLERINNQLEILEINKSILIRVTGCPNGCARPYMAELALVGSGLNQYQLWLGGS
HHHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
TNLKRLATPYLQKMPIDDLEKTLEPLFLSWKDTGASSSLGDHVTKLGSESVMSLLTSSAA
CCHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHCCC
P
C
>Mature Secondary Structure
MNFDEKESTNIEEIEQTVPSPCIANNSPRAKFEQFKADSNYLNEPLHSELLNESDHFTND
CCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHCCHHHHHHCCCCCCCHHH
AVQLLKFHGSYQQDDREHRKRGGTGKDWQMMLRLRNPAGYVPGPLFVALDELSDRLGNQT
HHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCHH
LRATTRQCFQMHGIKKRNIKEVIGTIVKSMGSTLAACGDVNRNVMAPAAPYEQGSYPAAR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
KLANDIADVLSPKKAEKTYIDLWVDGEMKYAIKPSSEVKKNRKLQLKPGVFSGDKKEPLY
HHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCHHHHCCCCEEECCCCCCCCCCCCCC
GATYLPRKFKCATTVPGDNSVDILTHDIGLVTFTNKKGVLEGCNVYVGGGMGRTHNLDTT
CCCCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCCCCCCEEECCCCCCCCCCHHH
FARIADPIGYVEGEHILELVQSILALQRDYGDRKTRRHSRLKYVLHDMGVDWFKKQLTSK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YFTRKIENLKHEGDTILEDYLGWHQQSEKLWFVGLPLLSGRLTGRVKKELRNIVEKFALD
HHHHHHHHHHCCCHHHHHHHHCHHCCCCCEEEEECHHHCCHHHHHHHHHHHHHHHHHHEE
VRLTPNQDLLLCNIGNYQKASVKRALINIGFMNPGAPDPLARNAMACPALPLCGLAMTEA
EEECCCCCEEEEECCCCHHHHHHHHHHHEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHH
ERFLPELLERINNQLEILEINKSILIRVTGCPNGCARPYMAELALVGSGLNQYQLWLGGS
HHHHHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
TNLKRLATPYLQKMPIDDLEKTLEPLFLSWKDTGASSSLGDHVTKLGSESVMSLLTSSAA
CCHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]