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The map label for this gene is cspR [C]

Identifier: 124025726

GI number: 124025726

Start: 944837

End: 945337

Strand: Direct

Name: cspR [C]

Synonym: NATL1_10191

Alternate gene names: 124025726

Gene position: 944837-945337 (Clockwise)

Preceding gene: 124025725

Following gene: 124025728

Centisome position: 50.67

GC content: 30.54

Gene sequence:

>501_bases
ATGAATTCATTAAATCGAACAAGACCTCGTGTCTCATTATTTGAACCAAGAATTCCACAGAACACTGGAACAATTGGTAG
AACATGCTTAGCCTTTAATATGTCATTAGATATTATTAAGCCAACTGGATTTAGCTTCGAAGATAAATATTTAAAAAGAG
CAGGTTTAGATTATTGGCCTCACGTAGATGTACATTTATATGAATCGTTTGATCAATATAAAAATTCATTTAAAAACTCA
AGAATTATAGCTTTTACAAAAAAAAGTAATAATTTAATTTCAAATATTATTTATAAAGATACAGATATACTATTATTTGG
ACGAGAGGATACAGGTTTGCCCGAAAGCATAATTAATAAGTGTGAAATTGTCGCAGGAATACCTATGCCAGGAGGTGAGA
ATCAATTAAAGACAGGGGGAGTTAGAAGTTTGAATTTATCCGTTGCCAGTGGTATCGTTTGTTATTATGCATGTTTACAA
TTAAACTTATTAAGCAAGTAG

Upstream 100 bases:

>100_bases
ATAGCACGTTAACCTCATTGCTTAGTTGTCGTTCAAAAAAAAGGTTGCTTGCTATCAATGGGATTGCAATTGATTTAGAT
CAAATTGGCGAGCTCATACC

Downstream 100 bases:

>100_bases
AAAGTCATTATCCAAGATAATTACAATATAAAACCCCATCATTTATTACATTATCATCATCAACCTCTAACCATTCTTTG
TATTCATCATACAGAGCTTC

Product: SpoU family tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MNSLNRTRPRVSLFEPRIPQNTGTIGRTCLAFNMSLDIIKPTGFSFEDKYLKRAGLDYWPHVDVHLYESFDQYKNSFKNS
RIIAFTKKSNNLISNIIYKDTDILLFGREDTGLPESIINKCEIVAGIPMPGGENQLKTGGVRSLNLSVASGIVCYYACLQ
LNLLSK

Sequences:

>Translated_166_residues
MNSLNRTRPRVSLFEPRIPQNTGTIGRTCLAFNMSLDIIKPTGFSFEDKYLKRAGLDYWPHVDVHLYESFDQYKNSFKNS
RIIAFTKKSNNLISNIIYKDTDILLFGREDTGLPESIINKCEIVAGIPMPGGENQLKTGGVRSLNLSVASGIVCYYACLQ
LNLLSK
>Mature_166_residues
MNSLNRTRPRVSLFEPRIPQNTGTIGRTCLAFNMSLDIIKPTGFSFEDKYLKRAGLDYWPHVDVHLYESFDQYKNSFKNS
RIIAFTKKSNNLISNIIYKDTDILLFGREDTGLPESIINKCEIVAGIPMPGGENQLKTGGVRSLNLSVASGIVCYYACLQ
LNLLSK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=151, Percent_Identity=37.7483443708609, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18686; Mature: 18686

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLNRTRPRVSLFEPRIPQNTGTIGRTCLAFNMSLDIIKPTGFSFEDKYLKRAGLDYWP
CCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECEEEEECCCCCCHHHHHHHHCCCCCCC
HVDVHLYESFDQYKNSFKNSRIIAFTKKSNNLISNIIYKDTDILLFGREDTGLPESIINK
CCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHEECCCCEEEECCCCCCCCHHHHHH
CEIVAGIPMPGGENQLKTGGVRSLNLSVASGIVCYYACLQLNLLSK
EEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSLNRTRPRVSLFEPRIPQNTGTIGRTCLAFNMSLDIIKPTGFSFEDKYLKRAGLDYWP
CCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECEEEEECCCCCCHHHHHHHHCCCCCCC
HVDVHLYESFDQYKNSFKNSRIIAFTKKSNNLISNIIYKDTDILLFGREDTGLPESIINK
CCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHEECCCCEEEECCCCCCCCHHHHHH
CEIVAGIPMPGGENQLKTGGVRSLNLSVASGIVCYYACLQLNLLSK
EEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA