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The map label for this gene is pdhB [H]

Identifier: 124025658

GI number: 124025658

Start: 875727

End: 876716

Strand: Direct

Name: pdhB [H]

Synonym: NATL1_09511

Alternate gene names: 124025658

Gene position: 875727-876716 (Clockwise)

Preceding gene: 124025657

Following gene: 124025659

Centisome position: 46.96

GC content: 37.47

Gene sequence:

>990_bases
GTGAAAGGGACTCTTTTATTTAATGCTCTTCGAGAAGCAATTGATGAAGAAATGGGTAGAGATCCCTTGGTATGCGTGAT
GGGTGAGGACGTTGGACAATATGGCGGGTCTTATAAAGTGACGAAAGATTTATATGAAAAATATGGAGAGTTCAGAGTCT
TAGATACCCCTATTGCTGAAAATAGTTTTACTGGTATGGCTGTTGGTGCAGCCATGACAGGTTTAAGACCAATTGTGGAA
GGGATGAATATGGGTTTTTTGTTGCTTGCGTTTAATCAAATATCAAACAATATGGGAATGCTTAGATATACGAGCGGGGG
AAATTTTACTATTCCAACAGTTGTAAGAGGCCCTGGTGGTGTTGGAAGGCAATTAGGCGCTGAACACAGTCAAAGACTTG
AAGCCTATTTTCATGCAGTTCCTGGTATCAAAATTGTCGCTTGTAGTACTCCTACAAATGCTAAAGGTCTAATGAAAGCT
GCTATAAGGGACAATAATCCTGTTCTTTTCTTTGAACATGTTCTTCTATATAACCTCACAGAAGAGCTGCCTGAGGGTGA
TTATGTCTGTGCCCTAGATCAAGCCGATTTAGTAAAGCAGGGAAGCGACATTACTATTTTGACTTATTCGAGAATGCGTC
ACCACTGTTTAAAAGCAGTCGAACTTCTTGAGGCTAAGGGAATTGATGTCGAATTAATTGATTTAATTAGTCTTAAGCCT
TTTGATATGAATACAATCTCTGAATCAATTAAAAAAACTCATAGAGTCATTATTGTTGAGGAATGTATGAAAACAGGTGG
TATTGCAGCTGAATTGATGTCTTTGATTACTGAAAATTGCTTCGATGATTTAGATTCTCCTCCTGTTCGTTTAAGCAGTC
AGGATATTCCGACTCCTTATAATGGAAACCTAGAAAATTTAACCATTATTCAACCTCATCAGATAGTAGATGCTGCTGAA
AAAATTATTAAAAATGGTGGATTAGATTAA

Upstream 100 bases:

>100_bases
CCATATTGCTTTTATCGACTTTTGCCGCAGGTGGGTAATTAGGCACTGTTTGAGATAGTTTGGAATAACAAACCGGTAAT
CCGGACGATCTGTTAAAGCT

Downstream 100 bases:

>100_bases
TAATGGGTAGAAAGAATTACTGGTTTCTTTTCGTTATCCTTTTTGCAATATTTAGTATTTTTATATGTACTAATATTCCT
TTTCAATTAGGATTGGATTT

Product: pyruvate dehydrogenase E1 beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKGTLLFNALREAIDEEMGRDPLVCVMGEDVGQYGGSYKVTKDLYEKYGEFRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNFTIPTVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDNNPVLFFEHVLLYNLTEELPEGDYVCALDQADLVKQGSDITILTYSRMRHHCLKAVELLEAKGIDVELIDLISLKP
FDMNTISESIKKTHRVIIVEECMKTGGIAAELMSLITENCFDDLDSPPVRLSSQDIPTPYNGNLENLTIIQPHQIVDAAE
KIIKNGGLD

Sequences:

>Translated_329_residues
MKGTLLFNALREAIDEEMGRDPLVCVMGEDVGQYGGSYKVTKDLYEKYGEFRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNFTIPTVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDNNPVLFFEHVLLYNLTEELPEGDYVCALDQADLVKQGSDITILTYSRMRHHCLKAVELLEAKGIDVELIDLISLKP
FDMNTISESIKKTHRVIIVEECMKTGGIAAELMSLITENCFDDLDSPPVRLSSQDIPTPYNGNLENLTIIQPHQIVDAAE
KIIKNGGLD
>Mature_329_residues
MKGTLLFNALREAIDEEMGRDPLVCVMGEDVGQYGGSYKVTKDLYEKYGEFRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNFTIPTVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDNNPVLFFEHVLLYNLTEELPEGDYVCALDQADLVKQGSDITILTYSRMRHHCLKAVELLEAKGIDVELIDLISLKP
FDMNTISESIKKTHRVIIVEECMKTGGIAAELMSLITENCFDDLDSPPVRLSSQDIPTPYNGNLENLTIIQPHQIVDAAE
KIIKNGGLD

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=321, Percent_Identity=40.4984423676012, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI291084858, Length=321, Percent_Identity=38.3177570093458, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4557353, Length=321, Percent_Identity=36.1370716510903, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI34101272, Length=321, Percent_Identity=36.1370716510903, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17538422, Length=319, Percent_Identity=41.692789968652, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17506935, Length=323, Percent_Identity=36.2229102167183, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319698, Length=321, Percent_Identity=40.1869158878505, Blast_Score=253, Evalue=3e-68,
Organism=Drosophila melanogaster, GI21358145, Length=321, Percent_Identity=38.3177570093458, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24650940, Length=321, Percent_Identity=38.3177570093458, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI160714832, Length=321, Percent_Identity=37.0716510903427, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI160714828, Length=321, Percent_Identity=37.0716510903427, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24650943, Length=85, Percent_Identity=51.7647058823529, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650945, Length=85, Percent_Identity=51.7647058823529, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36074; Mature: 36074

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGTLLFNALREAIDEEMGRDPLVCVMGEDVGQYGGSYKVTKDLYEKYGEFRVLDTPIAE
CCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEHHHHHHHCCCEEEEECCCCC
NSFTGMAVGAAMTGLRPIVEGMNMGFLLLAFNQISNNMGMLRYTSGGNFTIPTVVRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEEECCCCEEECEEEECCCC
VGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAAIRDNNPVLFFEHVLLYNLT
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHH
EELPEGDYVCALDQADLVKQGSDITILTYSRMRHHCLKAVELLEAKGIDVELIDLISLKP
HHCCCCCEEEEECCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEHHEECCC
FDMNTISESIKKTHRVIIVEECMKTGGIAAELMSLITENCFDDLDSPPVRLSSQDIPTPY
CCHHHHHHHHHHHHCEEEHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCC
NGNLENLTIIQPHQIVDAAEKIIKNGGLD
CCCCCEEEEECCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKGTLLFNALREAIDEEMGRDPLVCVMGEDVGQYGGSYKVTKDLYEKYGEFRVLDTPIAE
CCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEHHHHHHHCCCEEEEECCCCC
NSFTGMAVGAAMTGLRPIVEGMNMGFLLLAFNQISNNMGMLRYTSGGNFTIPTVVRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEEECCCCEEECEEEECCCC
VGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAAIRDNNPVLFFEHVLLYNLT
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHH
EELPEGDYVCALDQADLVKQGSDITILTYSRMRHHCLKAVELLEAKGIDVELIDLISLKP
HHCCCCCEEEEECCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEHHEECCC
FDMNTISESIKKTHRVIIVEECMKTGGIAAELMSLITENCFDDLDSPPVRLSSQDIPTPY
CCHHHHHHHHHHHHCEEEHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCC
NGNLENLTIIQPHQIVDAAEKIIKNGGLD
CCCCCEEEEECCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]