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The map label for this gene is amyA [H]

Identifier: 124025621

GI number: 124025621

Start: 845213

End: 846964

Strand: Direct

Name: amyA [H]

Synonym: NATL1_09141

Alternate gene names: 124025621

Gene position: 845213-846964 (Clockwise)

Preceding gene: 124025619

Following gene: 124025622

Centisome position: 45.33

GC content: 33.9

Gene sequence:

>1752_bases
GTGTCTGAGTTGGATAGTGAATTAGACGAGCTGAGATTGTCTCTCAGAGAAATTTATCCTGAGCACTCTGAACAAGAAAT
CAATTCAGTGTGGTCGCAATTGTTGCAGATTCTTGATCCATTTTGTGTTAGCAAGGGCACTGATGAATTTGAGATCGAAT
CAATTTGGGATTCTTCAAGTGTTGTTTTGATTACTTACCCTGATTCAATTTATAGGAAAGATGAATCAACTTTAAAAACA
TTAACTGAATTCGTAAAAAATAGATTAGGTGGCCTTTCATCAGTCATACATGTTTTACCATTTCTTCCTTCTACAAGTGA
TGGAGGATTCGCTGTATCTAATCATGAAAAAATCGATGATACCTTTGGGAATTGGAATGATTTAAAAGATTTATCTAGTA
AGCATAAAATAATGGCAGATCTAGTTTTAAATCATGTCTCTTCTTCTCATCCATGGGTTCATCAATTTATAAAATCAGAG
GATCCAGGTCCGTCTTACATTGTTTCTCCTTCTGAGACTAATGTTTGGGAAAATGTGATAAGGCCCAGAAATACATCACT
CTTTACCAATATCAATACTAAACAAGGCTTTAAGAGTGTTTGGACAACCTTTGGACCAGATCAGATTGATGTTGATTGGA
GGAATCCACATATCTTTTTAGAGTTTTTGAAATTATTGGTTAGATACATAACTAATGGAGCTGACTGGATTCGACTTGAT
GCAATTGCATTTATTTGGAAGGAGCCACATACTACTTGTTTACATTTAGACCCAGTACACTCAATAGTTAAGCTGTTAAA
TAAATGTTTGAAGATTATAAAACCTTCGGCAGTATTAATTACCGAGACTAATGTGCCAGAGAAAGAAAACCTTTCTTATT
TGATTGAAGGAAATGAAGCTAATCTTGCATATAACTTTACTCTACCTCCTTTATTATTAGAAGCTATTTATACCGGTAAA
ACAGATTTATTGAAGAGTTGGTTGTCTACGTGGAAAGAGTTGCCAAGGCACACTTCTTTGCTCAACTTCACTTCTTCACA
CGATGGTATTGGATTACGAGCACTAGAAGGCATTATGGACGATCAGAGAATACATAATCTCTTGGTTGAATCAGAGAAAC
GAGGAGGATTAGTTAGTCATCGTCGCTTGTCAAATGGAGATGATCAACCTTATGAATTAAACATTAGTTGGTGGAGTGCG
ATGTCAAACGAAGGCTCCGATAAAACGGTATTTCAATTTGAGCGTTTTTTATTAAGTCAGCTTTTTACTTTATCCATAAA
AGGTGTTCCAGCTTTTTATCTTCCATCTGTATTAGCTTCTCCGAATGATATAGATTCTTTTAGGAAAACAGGGCAAAGAA
GAGATTTAAATCGAGAAAAATTTGAAGCTAACCAATTACTTGATGTACTTAAGAACTTTGATTCTCCCGCTAGTAAAAAT
ATTTCATACCTCACTCATATAGTTAAAGTCAGGTCAAGACTTAAAGCTTTTCATCCGGAGGCGAGTATGAAATGTATCTC
TACGAATATAGCTAATTGTATAATTCTTCAAAGAGGTTTGGATGAAGATACTGTCTATGTTATTTGTAATATGTCTAGTA
AATTCTTATCTATTTCTCCATTAAATCAAATTAATTCATTAGAATTAACCTCTGAAAAACGTTTACTAGATAATATTTCA
GGTTCTTATTTTAATACTGATACTTTTAAACTTAATCCTTATCAAGTCGTTTGGCTTACATTAGCTGATTAG

Upstream 100 bases:

>100_bases
AGTTGTGATTAAACCCTGCTGAAAGTCCATTCAAGAAAAAAGTTTTACAGATTCAAGGTGGGAGCTACGATGAGGTAGAG
ATGATCGTTTTCTTTAAAGA

Downstream 100 bases:

>100_bases
TTTTATGAAAAATAATTCTAAATATTGGATAGTTACAGATCTTGATGGCACTTTAATGGACGAAGATTACGATATAACTC
CTGCCAAAGAAACCTTAAAA

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MSELDSELDELRLSLREIYPEHSEQEINSVWSQLLQILDPFCVSKGTDEFEIESIWDSSSVVLITYPDSIYRKDESTLKT
LTEFVKNRLGGLSSVIHVLPFLPSTSDGGFAVSNHEKIDDTFGNWNDLKDLSSKHKIMADLVLNHVSSSHPWVHQFIKSE
DPGPSYIVSPSETNVWENVIRPRNTSLFTNINTKQGFKSVWTTFGPDQIDVDWRNPHIFLEFLKLLVRYITNGADWIRLD
AIAFIWKEPHTTCLHLDPVHSIVKLLNKCLKIIKPSAVLITETNVPEKENLSYLIEGNEANLAYNFTLPPLLLEAIYTGK
TDLLKSWLSTWKELPRHTSLLNFTSSHDGIGLRALEGIMDDQRIHNLLVESEKRGGLVSHRRLSNGDDQPYELNISWWSA
MSNEGSDKTVFQFERFLLSQLFTLSIKGVPAFYLPSVLASPNDIDSFRKTGQRRDLNREKFEANQLLDVLKNFDSPASKN
ISYLTHIVKVRSRLKAFHPEASMKCISTNIANCIILQRGLDEDTVYVICNMSSKFLSISPLNQINSLELTSEKRLLDNIS
GSYFNTDTFKLNPYQVVWLTLAD

Sequences:

>Translated_583_residues
MSELDSELDELRLSLREIYPEHSEQEINSVWSQLLQILDPFCVSKGTDEFEIESIWDSSSVVLITYPDSIYRKDESTLKT
LTEFVKNRLGGLSSVIHVLPFLPSTSDGGFAVSNHEKIDDTFGNWNDLKDLSSKHKIMADLVLNHVSSSHPWVHQFIKSE
DPGPSYIVSPSETNVWENVIRPRNTSLFTNINTKQGFKSVWTTFGPDQIDVDWRNPHIFLEFLKLLVRYITNGADWIRLD
AIAFIWKEPHTTCLHLDPVHSIVKLLNKCLKIIKPSAVLITETNVPEKENLSYLIEGNEANLAYNFTLPPLLLEAIYTGK
TDLLKSWLSTWKELPRHTSLLNFTSSHDGIGLRALEGIMDDQRIHNLLVESEKRGGLVSHRRLSNGDDQPYELNISWWSA
MSNEGSDKTVFQFERFLLSQLFTLSIKGVPAFYLPSVLASPNDIDSFRKTGQRRDLNREKFEANQLLDVLKNFDSPASKN
ISYLTHIVKVRSRLKAFHPEASMKCISTNIANCIILQRGLDEDTVYVICNMSSKFLSISPLNQINSLELTSEKRLLDNIS
GSYFNTDTFKLNPYQVVWLTLAD
>Mature_582_residues
SELDSELDELRLSLREIYPEHSEQEINSVWSQLLQILDPFCVSKGTDEFEIESIWDSSSVVLITYPDSIYRKDESTLKTL
TEFVKNRLGGLSSVIHVLPFLPSTSDGGFAVSNHEKIDDTFGNWNDLKDLSSKHKIMADLVLNHVSSSHPWVHQFIKSED
PGPSYIVSPSETNVWENVIRPRNTSLFTNINTKQGFKSVWTTFGPDQIDVDWRNPHIFLEFLKLLVRYITNGADWIRLDA
IAFIWKEPHTTCLHLDPVHSIVKLLNKCLKIIKPSAVLITETNVPEKENLSYLIEGNEANLAYNFTLPPLLLEAIYTGKT
DLLKSWLSTWKELPRHTSLLNFTSSHDGIGLRALEGIMDDQRIHNLLVESEKRGGLVSHRRLSNGDDQPYELNISWWSAM
SNEGSDKTVFQFERFLLSQLFTLSIKGVPAFYLPSVLASPNDIDSFRKTGQRRDLNREKFEANQLLDVLKNFDSPASKNI
SYLTHIVKVRSRLKAFHPEASMKCISTNIANCIILQRGLDEDTVYVICNMSSKFLSISPLNQINSLELTSEKRLLDNISG
SYFNTDTFKLNPYQVVWLTLAD

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI87081873, Length=527, Percent_Identity=32.4478178368121, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1790687, Length=208, Percent_Identity=27.4038461538462, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 66513; Mature: 66382

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELDSELDELRLSLREIYPEHSEQEINSVWSQLLQILDPFCVSKGTDEFEIESIWDSSS
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHCCCCCCEEEHEECCCCC
VVLITYPDSIYRKDESTLKTLTEFVKNRLGGLSSVIHVLPFLPSTSDGGFAVSNHEKIDD
EEEEECCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEECCCCHHHH
TFGNWNDLKDLSSKHKIMADLVLNHVSSSHPWVHQFIKSEDPGPSYIVSPSETNVWENVI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEECCCCCHHHHHHC
RPRNTSLFTNINTKQGFKSVWTTFGPDQIDVDWRNPHIFLEFLKLLVRYITNGADWIRLD
CCCCCEEEEECCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEH
AIAFIWKEPHTTCLHLDPVHSIVKLLNKCLKIIKPSAVLITETNVPEKENLSYLIEGNEA
HEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCC
NLAYNFTLPPLLLEAIYTGKTDLLKSWLSTWKELPRHTSLLNFTSSHDGIGLRALEGIMD
CEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHEEECCCCCCCCHHHHHHHHH
DQRIHNLLVESEKRGGLVSHRRLSNGDDQPYELNISWWSAMSNEGSDKTVFQFERFLLSQ
HHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHH
LFTLSIKGVPAFYLPSVLASPNDIDSFRKTGQRRDLNREKFEANQLLDVLKNFDSPASKN
HHHHHCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ISYLTHIVKVRSRLKAFHPEASMKCISTNIANCIILQRGLDEDTVYVICNMSSKFLSISP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCCEEEECC
LNQINSLELTSEKRLLDNISGSYFNTDTFKLNPYQVVWLTLAD
CCCCCCCEECHHHHHHHHCCCCCCCCCCEEECCEEEEEEEECC
>Mature Secondary Structure 
SELDSELDELRLSLREIYPEHSEQEINSVWSQLLQILDPFCVSKGTDEFEIESIWDSSS
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHCCCCCCEEEHEECCCCC
VVLITYPDSIYRKDESTLKTLTEFVKNRLGGLSSVIHVLPFLPSTSDGGFAVSNHEKIDD
EEEEECCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEECCCCHHHH
TFGNWNDLKDLSSKHKIMADLVLNHVSSSHPWVHQFIKSEDPGPSYIVSPSETNVWENVI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEECCCCCHHHHHHC
RPRNTSLFTNINTKQGFKSVWTTFGPDQIDVDWRNPHIFLEFLKLLVRYITNGADWIRLD
CCCCCEEEEECCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEH
AIAFIWKEPHTTCLHLDPVHSIVKLLNKCLKIIKPSAVLITETNVPEKENLSYLIEGNEA
HEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCC
NLAYNFTLPPLLLEAIYTGKTDLLKSWLSTWKELPRHTSLLNFTSSHDGIGLRALEGIMD
CEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHEEECCCCCCCCHHHHHHHHH
DQRIHNLLVESEKRGGLVSHRRLSNGDDQPYELNISWWSAMSNEGSDKTVFQFERFLLSQ
HHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHH
LFTLSIKGVPAFYLPSVLASPNDIDSFRKTGQRRDLNREKFEANQLLDVLKNFDSPASKN
HHHHHCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ISYLTHIVKVRSRLKAFHPEASMKCISTNIANCIILQRGLDEDTVYVICNMSSKFLSISP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCCEEEECC
LNQINSLELTSEKRLLDNISGSYFNTDTFKLNPYQVVWLTLAD
CCCCCCCEECHHHHHHHHCCCCCCCCCCEEECCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]