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The map label for this gene is mmsB [C]

Identifier: 124025605

GI number: 124025605

Start: 834559

End: 835422

Strand: Direct

Name: mmsB [C]

Synonym: NATL1_08981

Alternate gene names: 124025605

Gene position: 834559-835422 (Clockwise)

Preceding gene: 124025601

Following gene: 124025606

Centisome position: 44.75

GC content: 33.68

Gene sequence:

>864_bases
ATGAAACTAGCTTTTATTGGCCTTGGAGCTATTGGTAAGCCCATGTCTCAGCGCCTAATTGATAATGGCTATGATTTGAA
TTTGTATCAGAGAAATAAATTAAATAATGACTCAAAAAGAAAATATTTTTTTGACCCTATAGAGGCTGTTAGTGACTGCG
ATGGCCTTTTGATTTGCGTGACTGATGATAATGCAGTTGAATCTGTTTTGTTTGGGGATAATGGAGTATCAGGATCACTA
AAGCCTAATTCATTTGTTATTGATTTCTCTACAATAAGTCCTGATAAATCTATCTCAATACATAAACGATTAGGTGAACA
AAATATTTTTTATGTTGACTGTCCAGTTTCTGGAGGGACAGAGGGTGCTCATAACGGTTCACTATCTTTATTTATTGGTG
CAAGTGAAAAAGATTGTTTATCTTTTGAAAATATATTTCAAGTGCTAGGTAAATCAATTAATTATTTTAATGGTGTAGGT
AAAGGTCAACAAGTCAAAGCTCTTAATCAGATACTGGTTGCAGGAACATATGCAGCTGTTGCAGAGGCAATGGAATTAGG
GAAAATGCTTGAATTGCCAATGGATGATGTCGTTGCTGCTTTGAAAGTAGGAGCAGCGAATTCATGGCCATTAGAAAATA
GATCAAAAGCAATGTTGATTGATAAGCATCCATTGGGATTTAAATTAGAGTTGCATCATAAGGATTTATCTATTGCCACT
GAGTTAGCTAAATCTATAAATATTGATTTACCAATTGCATCAAAAGTAAAAGAAATGGAGCAACGATTAATGCAGACTGG
TTTAGGTGAATTAGATGTTTCTGTTCTTCATAGGTATATATCAAATAAAAGAATAAATCACTAG

Upstream 100 bases:

>100_bases
ATTTTCTTCTTTTGGCAGCATATTAATTTTTTATAGTAATAGTTCAAATCTTTGTTGTACTTATTACTCTTAGTTCGTTT
TGAATGAAAATCCAATTTTG

Downstream 100 bases:

>100_bases
AAATCCTGTAAATATTGGTTTTTGATAACTTACTTTATTTACAAATTCAAAGAAGTATGCTAAGTTGATATTAATTATTT
AATTCTTAAATTTGTTTTAT

Product: 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKLAFIGLGAIGKPMSQRLIDNGYDLNLYQRNKLNNDSKRKYFFDPIEAVSDCDGLLICVTDDNAVESVLFGDNGVSGSL
KPNSFVIDFSTISPDKSISIHKRLGEQNIFYVDCPVSGGTEGAHNGSLSLFIGASEKDCLSFENIFQVLGKSINYFNGVG
KGQQVKALNQILVAGTYAAVAEAMELGKMLELPMDDVVAALKVGAANSWPLENRSKAMLIDKHPLGFKLELHHKDLSIAT
ELAKSINIDLPIASKVKEMEQRLMQTGLGELDVSVLHRYISNKRINH

Sequences:

>Translated_287_residues
MKLAFIGLGAIGKPMSQRLIDNGYDLNLYQRNKLNNDSKRKYFFDPIEAVSDCDGLLICVTDDNAVESVLFGDNGVSGSL
KPNSFVIDFSTISPDKSISIHKRLGEQNIFYVDCPVSGGTEGAHNGSLSLFIGASEKDCLSFENIFQVLGKSINYFNGVG
KGQQVKALNQILVAGTYAAVAEAMELGKMLELPMDDVVAALKVGAANSWPLENRSKAMLIDKHPLGFKLELHHKDLSIAT
ELAKSINIDLPIASKVKEMEQRLMQTGLGELDVSVLHRYISNKRINH
>Mature_287_residues
MKLAFIGLGAIGKPMSQRLIDNGYDLNLYQRNKLNNDSKRKYFFDPIEAVSDCDGLLICVTDDNAVESVLFGDNGVSGSL
KPNSFVIDFSTISPDKSISIHKRLGEQNIFYVDCPVSGGTEGAHNGSLSLFIGASEKDCLSFENIFQVLGKSINYFNGVG
KGQQVKALNQILVAGTYAAVAEAMELGKMLELPMDDVVAALKVGAANSWPLENRSKAMLIDKHPLGFKLELHHKDLSIAT
ELAKSINIDLPIASKVKEMEQRLMQTGLGELDVSVLHRYISNKRINH

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=290, Percent_Identity=27.5862068965517, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI40556376, Length=287, Percent_Identity=24.0418118466899, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI145693186, Length=290, Percent_Identity=31.7241379310345, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1786719, Length=279, Percent_Identity=34.0501792114695, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790315, Length=284, Percent_Identity=28.8732394366197, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1789092, Length=284, Percent_Identity=29.2253521126761, Blast_Score=88, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17557316, Length=271, Percent_Identity=25.4612546125461, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24655230, Length=294, Percent_Identity=25.1700680272109, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19922568, Length=294, Percent_Identity=25.1700680272109, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=21.7857142857143, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31404; Mature: 31404

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAFIGLGAIGKPMSQRLIDNGYDLNLYQRNKLNNDSKRKYFFDPIEAVSDCDGLLICV
CEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCHHHHHCCCCCEEEEE
TDDNAVESVLFGDNGVSGSLKPNSFVIDFSTISPDKSISIHKRLGEQNIFYVDCPVSGGT
ECCCHHHEEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHCCCCCEEEEECCCCCCC
EGAHNGSLSLFIGASEKDCLSFENIFQVLGKSINYFNGVGKGQQVKALNQILVAGTYAAV
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AEAMELGKMLELPMDDVVAALKVGAANSWPLENRSKAMLIDKHPLGFKLELHHKDLSIAT
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCHHHHH
ELAKSINIDLPIASKVKEMEQRLMQTGLGELDVSVLHRYISNKRINH
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLAFIGLGAIGKPMSQRLIDNGYDLNLYQRNKLNNDSKRKYFFDPIEAVSDCDGLLICV
CEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCHHHHHCCCCCEEEEE
TDDNAVESVLFGDNGVSGSLKPNSFVIDFSTISPDKSISIHKRLGEQNIFYVDCPVSGGT
ECCCHHHEEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHCCCCCEEEEECCCCCCC
EGAHNGSLSLFIGASEKDCLSFENIFQVLGKSINYFNGVGKGQQVKALNQILVAGTYAAV
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AEAMELGKMLELPMDDVVAALKVGAANSWPLENRSKAMLIDKHPLGFKLELHHKDLSIAT
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCHHHHH
ELAKSINIDLPIASKVKEMEQRLMQTGLGELDVSVLHRYISNKRINH
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA