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The map label for this gene is hisH [H]

Identifier: 124025583

GI number: 124025583

Start: 813516

End: 814169

Strand: Direct

Name: hisH [H]

Synonym: NATL1_08761

Alternate gene names: 124025583

Gene position: 813516-814169 (Clockwise)

Preceding gene: 124025582

Following gene: 124025584

Centisome position: 43.63

GC content: 31.19

Gene sequence:

>654_bases
ATGAAGGTAGGTATAATTGATTATGGAGTAGGTAACTTAAGAAGTATTTCTAATGCTCTTAATGAAATAGGAGCTGAAAA
TATAGTATCAAGCAATAAGAAAGAATTGGAAGAATGTCAGCGACTAATACTTCCAGGAGTTGGAGCATTTAATCATGGTG
TTAATTCTCTGCGAGAAAGAGATCTAATAAATTTTATAAAAAATCAACATTTTAATAAAATACCTATCCTAGGAATATGC
TTGGGCATGCAACTTTTAACTGAAGCAAGCTATGAATTTGGTGAAACAAAAGGTTTAGAAATTATTGAAGGCAAAGTTAA
AAAGATTGAAGATATGAACAATCTCGAAAAAAGTCTAAGACTTCCTATCGTTGGTTGGTTTCACATCAAAGAAACAAATA
TTAAGGCTAAGAAAAATTATTTTTTAGATAAAGTGCTCACAAAAAGACTAAAAGGGGCGAAATACTATTTTATTCATTCT
TATAGTGTTAACAGTGAGTCAAATGATGTAATTGCTGTTACTGAACATTTACAAACTAAAACAACGGCGGTAATTGCAAA
AGGAAATACTATTGGCACTCAATTCCATCCAGAAAAAAGCGGGATCTATGGTTTAGAATTCTTAAAAAGCTTTATATTTG
CAGCCCTCAGCTAA

Upstream 100 bases:

>100_bases
AACCTTCTGCTATTGCTATCGCAGGCTCTTTACATTACAAGAAAGATACCGTAAGTAGTATAAAACAAGAAATTAATTCT
TTGTTTATACAAGAAAAAAA

Downstream 100 bases:

>100_bases
GAATGGAATACAGTATTTCTTTGAATGTTCTATTCTTTTATCATCTATATTGTTATTTGTTTATCTTTGGATCTTCAGCA
ATTTGAACACAGATTTCATT

Product: glutamine amidotransferase

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKVGIIDYGVGNLRSISNALNEIGAENIVSSNKKELEECQRLILPGVGAFNHGVNSLRERDLINFIKNQHFNKIPILGIC
LGMQLLTEASYEFGETKGLEIIEGKVKKIEDMNNLEKSLRLPIVGWFHIKETNIKAKKNYFLDKVLTKRLKGAKYYFIHS
YSVNSESNDVIAVTEHLQTKTTAVIAKGNTIGTQFHPEKSGIYGLEFLKSFIFAALS

Sequences:

>Translated_217_residues
MKVGIIDYGVGNLRSISNALNEIGAENIVSSNKKELEECQRLILPGVGAFNHGVNSLRERDLINFIKNQHFNKIPILGIC
LGMQLLTEASYEFGETKGLEIIEGKVKKIEDMNNLEKSLRLPIVGWFHIKETNIKAKKNYFLDKVLTKRLKGAKYYFIHS
YSVNSESNDVIAVTEHLQTKTTAVIAKGNTIGTQFHPEKSGIYGLEFLKSFIFAALS
>Mature_217_residues
MKVGIIDYGVGNLRSISNALNEIGAENIVSSNKKELEECQRLILPGVGAFNHGVNSLRERDLINFIKNQHFNKIPILGIC
LGMQLLTEASYEFGETKGLEIIEGKVKKIEDMNNLEKSLRLPIVGWFHIKETNIKAKKNYFLDKVLTKRLKGAKYYFIHS
YSVNSESNDVIAVTEHLQTKTTAVIAKGNTIGTQFHPEKSGIYGLEFLKSFIFAALS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=212, Percent_Identity=34.4339622641509, Blast_Score=109, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319725, Length=221, Percent_Identity=35.2941176470588, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 24335; Mature: 24335

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGIIDYGVGNLRSISNALNEIGAENIVSSNKKELEECQRLILPGVGAFNHGVNSLRER
CEEEEEECCCCHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
DLINFIKNQHFNKIPILGICLGMQLLTEASYEFGETKGLEIIEGKVKKIEDMNNLEKSLR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LPIVGWFHIKETNIKAKKNYFLDKVLTKRLKGAKYYFIHSYSVNSESNDVIAVTEHLQTK
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEHHHHHCH
TTAVIAKGNTIGTQFHPEKSGIYGLEFLKSFIFAALS
HEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVGIIDYGVGNLRSISNALNEIGAENIVSSNKKELEECQRLILPGVGAFNHGVNSLRER
CEEEEEECCCCHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
DLINFIKNQHFNKIPILGICLGMQLLTEASYEFGETKGLEIIEGKVKKIEDMNNLEKSLR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LPIVGWFHIKETNIKAKKNYFLDKVLTKRLKGAKYYFIHSYSVNSESNDVIAVTEHLQTK
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEHHHHHCH
TTAVIAKGNTIGTQFHPEKSGIYGLEFLKSFIFAALS
HEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA