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The map label for this gene is hisF [H]

Identifier: 124025582

GI number: 124025582

Start: 812773

End: 813519

Strand: Direct

Name: hisF [H]

Synonym: NATL1_08751

Alternate gene names: 124025582

Gene position: 812773-813519 (Clockwise)

Preceding gene: 124025581

Following gene: 124025583

Centisome position: 43.59

GC content: 32.4

Gene sequence:

>747_bases
ATGAGAATTATAGGAAGATTGGATGTAAAGAATAATCATGTTATAAAAGGGATTCACTTGGAAGGTCTTAGGAAAGTCGG
AGATCCACAAGAGTTAGCAGTAGAATACTATAAACAAGGAATTGAAGAAATAGTTTTCATGGATGCAGTCGCAAGTCTAT
ATAATAGAAATAATTTATTTCACATAATACAAAAGGCGTGTGAGAAAGTTTTTGTCCCAATTGCCTTAGGTGGCGGATTA
AGATCCTTGGATGATGTTTCTAAAGCTTTGAACGCAGGTGCAGATAAGGTAGTTATAAATACGGCATTAGTAAATAGAAT
TGATTTAGCTAAAGAAATAGCACAAAAGTATGGTTCGCAATGTTTAGTGGGTTCTATCGAAGCTAAAAGAGAAAATGATT
CTTGGAGAGTTTACGTTGATAATGGTAGAGAGCCAACTAAACACAATGTAGTTGACTGGGCAATCAAACTTAAAGAAGCT
GGTGTAGGAGAAATACTTCTAACATCTATTGATCAAGAAGGAACTTCAAAGGGTTTTGATATTGAACTTATAAAACAAAT
AAATGAATCAATAAGATGTCCAATAATTGCAAGTGGAGGTTATGGAAATAAAAAACATCTTGCTGATTTATTAAAAATTG
TTGAACCTTCTGCTATTGCTATCGCAGGCTCTTTACATTACAAGAAAGATACCGTAAGTAGTATAAAACAAGAAATTAAT
TCTTTGTTTATACAAGAAAAAAAATGA

Upstream 100 bases:

>100_bases
AGAAAGATCTATAGACATTGATAATGAATCAGATTTTGAAAAAGTCTCTACATTTATGAATATTTAGTTTATAGTAAAAT
ATCTATAAAATAGAATTGAA

Downstream 100 bases:

>100_bases
AGGTAGGTATAATTGATTATGGAGTAGGTAACTTAAGAAGTATTTCTAATGCTCTTAATGAAATAGGAGCTGAAAATATA
GTATCAAGCAATAAGAAAGA

Product: imidazole glycerol-phosphate synthase

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRIIGRLDVKNNHVIKGIHLEGLRKVGDPQELAVEYYKQGIEEIVFMDAVASLYNRNNLFHIIQKACEKVFVPIALGGGL
RSLDDVSKALNAGADKVVINTALVNRIDLAKEIAQKYGSQCLVGSIEAKRENDSWRVYVDNGREPTKHNVVDWAIKLKEA
GVGEILLTSIDQEGTSKGFDIELIKQINESIRCPIIASGGYGNKKHLADLLKIVEPSAIAIAGSLHYKKDTVSSIKQEIN
SLFIQEKK

Sequences:

>Translated_248_residues
MRIIGRLDVKNNHVIKGIHLEGLRKVGDPQELAVEYYKQGIEEIVFMDAVASLYNRNNLFHIIQKACEKVFVPIALGGGL
RSLDDVSKALNAGADKVVINTALVNRIDLAKEIAQKYGSQCLVGSIEAKRENDSWRVYVDNGREPTKHNVVDWAIKLKEA
GVGEILLTSIDQEGTSKGFDIELIKQINESIRCPIIASGGYGNKKHLADLLKIVEPSAIAIAGSLHYKKDTVSSIKQEIN
SLFIQEKK
>Mature_248_residues
MRIIGRLDVKNNHVIKGIHLEGLRKVGDPQELAVEYYKQGIEEIVFMDAVASLYNRNNLFHIIQKACEKVFVPIALGGGL
RSLDDVSKALNAGADKVVINTALVNRIDLAKEIAQKYGSQCLVGSIEAKRENDSWRVYVDNGREPTKHNVVDWAIKLKEA
GVGEILLTSIDQEGTSKGFDIELIKQINESIRCPIIASGGYGNKKHLADLLKIVEPSAIAIAGSLHYKKDTVSSIKQEIN
SLFIQEKK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=234, Percent_Identity=31.6239316239316, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87082028, Length=187, Percent_Identity=26.7379679144385, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319725, Length=300, Percent_Identity=28.6666666666667, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27417; Mature: 27417

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIGRLDVKNNHVIKGIHLEGLRKVGDPQELAVEYYKQGIEEIVFMDAVASLYNRNNLF
CCEEEEEECCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
HIIQKACEKVFVPIALGGGLRSLDDVSKALNAGADKVVINTALVNRIDLAKEIAQKYGSQ
HHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCE
CLVGSIEAKRENDSWRVYVDNGREPTKHNVVDWAIKLKEAGVGEILLTSIDQEGTSKGFD
EEEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHEECCCCCCCCEEEEECCCCCCCCCCC
IELIKQINESIRCPIIASGGYGNKKHLADLLKIVEPSAIAIAGSLHYKKDTVSSIKQEIN
HHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
SLFIQEKK
HHHHCCCC
>Mature Secondary Structure
MRIIGRLDVKNNHVIKGIHLEGLRKVGDPQELAVEYYKQGIEEIVFMDAVASLYNRNNLF
CCEEEEEECCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
HIIQKACEKVFVPIALGGGLRSLDDVSKALNAGADKVVINTALVNRIDLAKEIAQKYGSQ
HHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCE
CLVGSIEAKRENDSWRVYVDNGREPTKHNVVDWAIKLKEAGVGEILLTSIDQEGTSKGFD
EEEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHEECCCCCCCCEEEEECCCCCCCCCCC
IELIKQINESIRCPIIASGGYGNKKHLADLLKIVEPSAIAIAGSLHYKKDTVSSIKQEIN
HHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
SLFIQEKK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11043980 [H]