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The map label for this gene is hisA

Identifier: 124025543

GI number: 124025543

Start: 768731

End: 769501

Strand: Reverse

Name: hisA

Synonym: NATL1_08361

Alternate gene names: 124025543

Gene position: 769501-768731 (Counterclockwise)

Preceding gene: 124025545

Following gene: 124025542

Centisome position: 41.27

GC content: 36.06

Gene sequence:

>771_bases
ATGGAAATCATACCTGCAATAGATCTACTGAATGGTAAATGTGTTCGACTAAATCAAGGAAATTATAATGAAGTTACTAA
GTTCAATAGTGATCCTGTAAAACAAGCACAAATTTGGGAAAGCAAGGGAGCAAAACGACTACATCTTGTAGATCTTGATG
GTGCTAAGACAGGTGAGCCCATAAATGATCTAACTATAAAAGAGATAAAAAAATCTATTACAATACCTATTCAACTTGGC
GGTGGAATTAGGAGTATTGATCGTGCGAAAGAATTATTCGACATTGGAATAGACAGAATTATTTTAGGAACAATTGCAAT
AGAGAAGCCCGAATTAGTTAAAGACCTATCTAAAGAATATCCAAAAAGAGTTGCAGTAGGAATTGATGCCAAAGAGGGAA
TGGTAGCCACTCGAGGTTGGTTAAAACAAAGCAAAATATCTTCTCTAGACTTAGCAAAACAACTTAACGATCTTGACTTA
GCGGCAATCATATCAACTGACATTGCTACCGATGGCACTCTAAAAGGACCTAATGTTCAAGCCTTGAGAGAAATAGCTGA
GATAAGTATTAATCCAGTAATTGCCTCAGGGGGGATAGGTTCAATAGCTGATTTAATTTCACTAGCAGATTTCGCGGATG
AAGGTATTGAAGGAATAATCGTAGGCAGAGCCCTATATGACGGCTCAATAGATTTAAAGGAAGCGATTTTAACTCTAAAA
AATCTTCTTCTGCAAGATGCTTTCAATGAGAAAGATAAATTTCTTGTCTAA

Upstream 100 bases:

>100_bases
CTTTCAAGATCATTTATTAAAAAAATTATATTAAAGCCTATAGATAATCTCCCAGAGCTACATAAATGATGTTAGATGAA
TAGTAATAATTTTTTTCTTG

Downstream 100 bases:

>100_bases
CATTTAAAAAAATGTTCAATCCTTTGGATTTTGGAAATAGGGCTGGAAGTATTTTTAAATCAAGTTATCTTAAAAAGGAT
AGGTTCTAGATTTAAAATTG

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MEIIPAIDLLNGKCVRLNQGNYNEVTKFNSDPVKQAQIWESKGAKRLHLVDLDGAKTGEPINDLTIKEIKKSITIPIQLG
GGIRSIDRAKELFDIGIDRIILGTIAIEKPELVKDLSKEYPKRVAVGIDAKEGMVATRGWLKQSKISSLDLAKQLNDLDL
AAIISTDIATDGTLKGPNVQALREIAEISINPVIASGGIGSIADLISLADFADEGIEGIIVGRALYDGSIDLKEAILTLK
NLLLQDAFNEKDKFLV

Sequences:

>Translated_256_residues
MEIIPAIDLLNGKCVRLNQGNYNEVTKFNSDPVKQAQIWESKGAKRLHLVDLDGAKTGEPINDLTIKEIKKSITIPIQLG
GGIRSIDRAKELFDIGIDRIILGTIAIEKPELVKDLSKEYPKRVAVGIDAKEGMVATRGWLKQSKISSLDLAKQLNDLDL
AAIISTDIATDGTLKGPNVQALREIAEISINPVIASGGIGSIADLISLADFADEGIEGIIVGRALYDGSIDLKEAILTLK
NLLLQDAFNEKDKFLV
>Mature_256_residues
MEIIPAIDLLNGKCVRLNQGNYNEVTKFNSDPVKQAQIWESKGAKRLHLVDLDGAKTGEPINDLTIKEIKKSITIPIQLG
GGIRSIDRAKELFDIGIDRIILGTIAIEKPELVKDLSKEYPKRVAVGIDAKEGMVATRGWLKQSKISSLDLAKQLNDLDL
AAIISTDIATDGTLKGPNVQALREIAEISINPVIASGGIGSIADLISLADFADEGIEGIIVGRALYDGSIDLKEAILTLK
NLLLQDAFNEKDKFLV

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=248, Percent_Identity=35.0806451612903, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1788336, Length=243, Percent_Identity=23.8683127572016, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_PROM1 (A2C1N4)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014659.1
- ProteinModelPortal:   A2C1N4
- SMR:   A2C1N4
- STRING:   A2C1N4
- GeneID:   4780472
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_08361
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   PMAR167555:NATL1_08361-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 27721; Mature: 27721

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIPAIDLLNGKCVRLNQGNYNEVTKFNSDPVKQAQIWESKGAKRLHLVDLDGAKTGEP
CCCCCCEECCCCEEEEECCCCCCHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
INDLTIKEIKKSITIPIQLGGGIRSIDRAKELFDIGIDRIILGTIAIEKPELVKDLSKEY
CCHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHC
PKRVAVGIDAKEGMVATRGWLKQSKISSLDLAKQLNDLDLAAIISTDIATDGTLKGPNVQ
CHHEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHH
ALREIAEISINPVIASGGIGSIADLISLADFADEGIEGIIVGRALYDGSIDLKEAILTLK
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHH
NLLLQDAFNEKDKFLV
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEIIPAIDLLNGKCVRLNQGNYNEVTKFNSDPVKQAQIWESKGAKRLHLVDLDGAKTGEP
CCCCCCEECCCCEEEEECCCCCCHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
INDLTIKEIKKSITIPIQLGGGIRSIDRAKELFDIGIDRIILGTIAIEKPELVKDLSKEY
CCHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHC
PKRVAVGIDAKEGMVATRGWLKQSKISSLDLAKQLNDLDLAAIISTDIATDGTLKGPNVQ
CHHEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHH
ALREIAEISINPVIASGGIGSIADLISLADFADEGIEGIIVGRALYDGSIDLKEAILTLK
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHH
NLLLQDAFNEKDKFLV
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA