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The map label for this gene is proB [H]

Identifier: 124025535

GI number: 124025535

Start: 761482

End: 762564

Strand: Reverse

Name: proB [H]

Synonym: NATL1_08281

Alternate gene names: 124025535

Gene position: 762564-761482 (Counterclockwise)

Preceding gene: 124025536

Following gene: 124025534

Centisome position: 40.89

GC content: 34.72

Gene sequence:

>1083_bases
ATGACAACCTGGGTAATCAAGATTGGCACAAGCCTTTTGAGAGGAAACGATAAATATACAACTTTTGATGTAATTAATGA
TTATTGTTCTTATATATCAAAAGCTCAAAGGAATGGCGATAAAATTATATTGGTTTCTAGTGGTGCTGTAGGACTTGGAT
GTCATCAAATGAGATTCAAGACAAGACCTAAAGAAATAATCTCTCTTCAAGCTTCTGCCGCAATAGGCCAACTTCATTTA
ATGGCTTTATATGAAAAGGCTATGAGCATTTTTGGATATAAAGTCGCACAAATATTATTAACTAGATCAGAATTAGGCTC
AAGAAATAGTTATAACTCTGCTTCGCAAACATTAAAAAGATTGCTCGAATGGGATGTTATACCAATCGTAAACGAAAATG
ATATAACCTCAGATGAAGAGCTGAAGTATGGTGATAATGATACTTTATCTGCATTAGTTGCAACAGCTATATCTGCTGAT
CAATTAATATTATTAACTGATATCGATCATCTCTACTCTTCTGATCCCAAAATCAATAGTAAAGCTAAGCCAATCAAAGA
TATTAACAATTCAAAAGAATTAAATAATTTAGAACTAGCAAATGAACAAACTTCATGGGGGACTGGAGGAATAAAAACAA
AATTAACTGCTGCAAAGATCGCCACTGAAAGCGGGATAAAAGTTCAATTAGCAGATGGCAGAGATCCGAAAATATTAGGG
GAATTACTTGATGGAAAAAAAATAGGAACTGTTTTTCACCCCAATCCAAACCCTATCGGGAATAGAAAAAGTTGGTTGGC
ACATGCAATTAAACCAGTCGGAGAAATACACTTAGATGATGGTGCTAGTGAAGCTATCAAAAACAAAGGTGCTTCACTAT
TATTAGTTGGGGTGAAAAAAGTTAGCGGGAATTTTATTGCGAATCAACCTGTGAAAGTTATTAACACGGAAGGAGAGGAA
ATTGCTAAAGGAATTTGCTCAATGAGCAGTGATGCTATAAAGATAGGAATTAATTCAAGATCCGAAACAACAGGATCTCC
TCTAGTTATCCACCGAGATGTTCTGGTTCTAACAAGTGAATAA

Upstream 100 bases:

>100_bases
TATTGGTAGATTCGGTAGATTATTATGGAAAAAGAATTGAAAAAAATAATTTTCAATCCTTAGAAAGATACTTAGCAAGA
ATTATAACTGGAGACATTTC

Downstream 100 bases:

>100_bases
CTCAGATAACAATACTAATTTTCACTTCCCCAATCAAATCAAATCATGCAATTCATTGAAATTGTCGAAAAACTGAAGAA
AGGTCAATCTGGTGTCGTTG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTTWVIKIGTSLLRGNDKYTTFDVINDYCSYISKAQRNGDKIILVSSGAVGLGCHQMRFKTRPKEIISLQASAAIGQLHL
MALYEKAMSIFGYKVAQILLTRSELGSRNSYNSASQTLKRLLEWDVIPIVNENDITSDEELKYGDNDTLSALVATAISAD
QLILLTDIDHLYSSDPKINSKAKPIKDINNSKELNNLELANEQTSWGTGGIKTKLTAAKIATESGIKVQLADGRDPKILG
ELLDGKKIGTVFHPNPNPIGNRKSWLAHAIKPVGEIHLDDGASEAIKNKGASLLLVGVKKVSGNFIANQPVKVINTEGEE
IAKGICSMSSDAIKIGINSRSETTGSPLVIHRDVLVLTSE

Sequences:

>Translated_360_residues
MTTWVIKIGTSLLRGNDKYTTFDVINDYCSYISKAQRNGDKIILVSSGAVGLGCHQMRFKTRPKEIISLQASAAIGQLHL
MALYEKAMSIFGYKVAQILLTRSELGSRNSYNSASQTLKRLLEWDVIPIVNENDITSDEELKYGDNDTLSALVATAISAD
QLILLTDIDHLYSSDPKINSKAKPIKDINNSKELNNLELANEQTSWGTGGIKTKLTAAKIATESGIKVQLADGRDPKILG
ELLDGKKIGTVFHPNPNPIGNRKSWLAHAIKPVGEIHLDDGASEAIKNKGASLLLVGVKKVSGNFIANQPVKVINTEGEE
IAKGICSMSSDAIKIGINSRSETTGSPLVIHRDVLVLTSE
>Mature_359_residues
TTWVIKIGTSLLRGNDKYTTFDVINDYCSYISKAQRNGDKIILVSSGAVGLGCHQMRFKTRPKEIISLQASAAIGQLHLM
ALYEKAMSIFGYKVAQILLTRSELGSRNSYNSASQTLKRLLEWDVIPIVNENDITSDEELKYGDNDTLSALVATAISADQ
LILLTDIDHLYSSDPKINSKAKPIKDINNSKELNNLELANEQTSWGTGGIKTKLTAAKIATESGIKVQLADGRDPKILGE
LLDGKKIGTVFHPNPNPIGNRKSWLAHAIKPVGEIHLDDGASEAIKNKGASLLLVGVKKVSGNFIANQPVKVINTEGEEI
AKGICSMSSDAIKIGINSRSETTGSPLVIHRDVLVLTSE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=281, Percent_Identity=29.1814946619217, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI21361368, Length=283, Percent_Identity=28.9752650176678, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1786437, Length=361, Percent_Identity=37.1191135734072, Blast_Score=254, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17569969, Length=317, Percent_Identity=28.391167192429, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17569967, Length=317, Percent_Identity=28.391167192429, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320506, Length=344, Percent_Identity=35.7558139534884, Blast_Score=179, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321822, Length=262, Percent_Identity=38.9312977099237, Blast_Score=166, Evalue=5e-42,
Organism=Drosophila melanogaster, GI21357643, Length=280, Percent_Identity=28.9285714285714, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39023; Mature: 38892

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00018 EF_HAND_1 ; PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTWVIKIGTSLLRGNDKYTTFDVINDYCSYISKAQRNGDKIILVSSGAVGLGCHQMRFK
CCEEEEEECHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHC
TRPKEIISLQASAAIGQLHLMALYEKAMSIFGYKVAQILLTRSELGSRNSYNSASQTLKR
CCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LLEWDVIPIVNENDITSDEELKYGDNDTLSALVATAISADQLILLTDIDHLYSSDPKINS
HHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHCCCCCCCC
KAKPIKDINNSKELNNLELANEQTSWGTGGIKTKLTAAKIATESGIKVQLADGRDPKILG
CCCCHHHCCCCCCCCCEEECCCCCCCCCCCCEEEEEHHHHCCCCCCEEEECCCCCHHHHH
ELLDGKKIGTVFHPNPNPIGNRKSWLAHAIKPVGEIHLDDGASEAIKNKGASLLLVGVKK
HHHCCCEECEEECCCCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHCCCCEEEEEEEEE
VSGNFIANQPVKVINTEGEEIAKGICSMSSDAIKIGINSRSETTGSPLVIHRDVLVLTSE
CCCCEECCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEEEEEECC
>Mature Secondary Structure 
TTWVIKIGTSLLRGNDKYTTFDVINDYCSYISKAQRNGDKIILVSSGAVGLGCHQMRFK
CEEEEEECHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHC
TRPKEIISLQASAAIGQLHLMALYEKAMSIFGYKVAQILLTRSELGSRNSYNSASQTLKR
CCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LLEWDVIPIVNENDITSDEELKYGDNDTLSALVATAISADQLILLTDIDHLYSSDPKINS
HHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHCCCCCCCC
KAKPIKDINNSKELNNLELANEQTSWGTGGIKTKLTAAKIATESGIKVQLADGRDPKILG
CCCCHHHCCCCCCCCCEEECCCCCCCCCCCCEEEEEHHHHCCCCCCEEEECCCCCHHHHH
ELLDGKKIGTVFHPNPNPIGNRKSWLAHAIKPVGEIHLDDGASEAIKNKGASLLLVGVKK
HHHCCCEECEEECCCCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHCCCCEEEEEEEEE
VSGNFIANQPVKVINTEGEEIAKGICSMSSDAIKIGINSRSETTGSPLVIHRDVLVLTSE
CCCCEECCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA