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The map label for this gene is ilvD [H]

Identifier: 124025519

GI number: 124025519

Start: 748362

End: 750032

Strand: Reverse

Name: ilvD [H]

Synonym: NATL1_08121

Alternate gene names: 124025519

Gene position: 750032-748362 (Counterclockwise)

Preceding gene: 124025520

Following gene: 124025511

Centisome position: 40.22

GC content: 41.53

Gene sequence:

>1671_bases
ATGCTTAGATCAAATGCAATAACACAGGGTATTCAACGATCACCAAACCGAGCAATGCTTAGAGCTGTTGGGTTTGATGA
TAATGATTTTAATAAGCCAATCATTGGAATCGCTAATGGGCACAGCACAATAACCCCATGCAATATGGGATTAATGGATT
TAGCTAATAGAGCAGAGTCAGCTTTAAAAGAGGCTGGTGCAATGCCTCAAACTTTCGGAACCATAACTGTTAGTGACGGC
ATTTCCATGGGAACAGAAGGGATGAAATACTCATTAGTTTCAAGAGAAGTTATTGCAGATTCAATCGAGACGGCTTGCAA
TGCTCAAAGCATGGATGGGGTGTTAGCTATAGGTGGATGTGACAAAAATATGCCAGGAGCAATGTTATCAATGGCAAGAA
TGAACATTCCTTCGATTTTTGTTTATGGGGGGACAATTAAACCTGGGAAATTAGACGGTTGCGACTTGACCGTGGTGAGT
TCATTTGAGGCTGTCGGTCAATTAGCAAGCGGAAAAATCGACAAAGATCGTTTAATAGCTGTAGAAAAAAATGCTATCCC
TGGACCAGGAAGTTGCGGAGGGATGTTTACTGCTAACACCATGTCTGCGGCAATCGAAACGATGGGGTTTAGTTTGCCAT
TTAGTTCAACAATGGCTGCAGTAGATGATGAAAAAGCAGAAAGCGCTGCTGAAAGTGCTCAGGTCCTTGTCAATGCAGTA
AAGAACAACATCAGACCATTAGATTTACTTACAAAAGAAGCCTTTGAGAATGCAATCAGTGTCATCATGGCTGTAGGCGG
CTCAACAAATTCTGTCCTTCACCTACTTGCTATAGCTAGGACTGCAGGAGTTGATTTAACAATAGATGACTTTGAACGTA
TAAGGCAGACTGTTCCTGTGATTTGCGACCTCAAACCAAGCGGTAAATACGTAACAGTCGACCTACATAAAGCTGGTGGG
ATTCCTCAAGTAATGAAAATACTCCTCGATGCAGGAATGCTCCATGGAGAATGCAAAACCATTGAAGGAAAAACAATTAA
GGAAGTCCTTAGAGATATCCCCTCAAAGCCCAAAGAAAATCAAGACGTGATAAGACAAATATCAAACCCTATTTATAAAA
AAGGACATCTAGCTATTCTCAAAGGAAATTTAGCAAGTGAAGGAAGTGTCGCAAAAATTAGTGGTGTGAAGACTCCCGTT
TTAACCGGGCCTGCAAGGGTCTTTGAGAGTGAAGAAGAATGCTTAACTGCCATTCTTGACAATAAAGTTAAAGCTGGAGA
TGTAGTCGTCGTTAGATATGAAGGACCTGTAGGAGGGCCTGGCATGAGAGAGATGCTCTCTCCAACCTCAGCAATTGTAG
GTCAAGGATTAGGAGAGAAAGTTGCACTAATTACTGATGGTCGATTTAGTGGCGGATCATATGGATTAGTGGTTGGACAC
GTTGCTCCAGAAGCAGCCGTCGGAGGAACAATTGGATTAGTAGAGGAAGGAGACAGTATTACCGTTGACGCTAATAAATT
GTTAATTCAATTAAATGTCGAAGAGCGAGAATTAGCTAGAAGGAAAGAAAAATGGGAGAAGCCAAAGCCTAGATATAAGA
CAGGCATTCTTGGGAAGTATTCCAGATTAGTAAGTTCATCAAGCCAAGGAGCGACAACTGATCAAATATAA

Upstream 100 bases:

>100_bases
TGAACAGCTTAATTCGGCCATAGCAGACGTTCGCAAACAATTAGAAGACCTAGGATCTTCCAATTGATACTAATTTTCTA
AACAATTAAATAGAAATCAA

Downstream 100 bases:

>100_bases
CTCAGGACAATGAAGTCTTAAAAAACGTCCTTAGTCTTCTTGCAAGGGACTCAAAATGATTTCCATATGAAGGCTTTTCG
CATTTCACACCCGAAGAGCT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MLRSNAITQGIQRSPNRAMLRAVGFDDNDFNKPIIGIANGHSTITPCNMGLMDLANRAESALKEAGAMPQTFGTITVSDG
ISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMLSMARMNIPSIFVYGGTIKPGKLDGCDLTVVS
SFEAVGQLASGKIDKDRLIAVEKNAIPGPGSCGGMFTANTMSAAIETMGFSLPFSSTMAAVDDEKAESAAESAQVLVNAV
KNNIRPLDLLTKEAFENAISVIMAVGGSTNSVLHLLAIARTAGVDLTIDDFERIRQTVPVICDLKPSGKYVTVDLHKAGG
IPQVMKILLDAGMLHGECKTIEGKTIKEVLRDIPSKPKENQDVIRQISNPIYKKGHLAILKGNLASEGSVAKISGVKTPV
LTGPARVFESEEECLTAILDNKVKAGDVVVVRYEGPVGGPGMREMLSPTSAIVGQGLGEKVALITDGRFSGGSYGLVVGH
VAPEAAVGGTIGLVEEGDSITVDANKLLIQLNVEERELARRKEKWEKPKPRYKTGILGKYSRLVSSSSQGATTDQI

Sequences:

>Translated_556_residues
MLRSNAITQGIQRSPNRAMLRAVGFDDNDFNKPIIGIANGHSTITPCNMGLMDLANRAESALKEAGAMPQTFGTITVSDG
ISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMLSMARMNIPSIFVYGGTIKPGKLDGCDLTVVS
SFEAVGQLASGKIDKDRLIAVEKNAIPGPGSCGGMFTANTMSAAIETMGFSLPFSSTMAAVDDEKAESAAESAQVLVNAV
KNNIRPLDLLTKEAFENAISVIMAVGGSTNSVLHLLAIARTAGVDLTIDDFERIRQTVPVICDLKPSGKYVTVDLHKAGG
IPQVMKILLDAGMLHGECKTIEGKTIKEVLRDIPSKPKENQDVIRQISNPIYKKGHLAILKGNLASEGSVAKISGVKTPV
LTGPARVFESEEECLTAILDNKVKAGDVVVVRYEGPVGGPGMREMLSPTSAIVGQGLGEKVALITDGRFSGGSYGLVVGH
VAPEAAVGGTIGLVEEGDSITVDANKLLIQLNVEERELARRKEKWEKPKPRYKTGILGKYSRLVSSSSQGATTDQI
>Mature_556_residues
MLRSNAITQGIQRSPNRAMLRAVGFDDNDFNKPIIGIANGHSTITPCNMGLMDLANRAESALKEAGAMPQTFGTITVSDG
ISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMLSMARMNIPSIFVYGGTIKPGKLDGCDLTVVS
SFEAVGQLASGKIDKDRLIAVEKNAIPGPGSCGGMFTANTMSAAIETMGFSLPFSSTMAAVDDEKAESAAESAQVLVNAV
KNNIRPLDLLTKEAFENAISVIMAVGGSTNSVLHLLAIARTAGVDLTIDDFERIRQTVPVICDLKPSGKYVTVDLHKAGG
IPQVMKILLDAGMLHGECKTIEGKTIKEVLRDIPSKPKENQDVIRQISNPIYKKGHLAILKGNLASEGSVAKISGVKTPV
LTGPARVFESEEECLTAILDNKVKAGDVVVVRYEGPVGGPGMREMLSPTSAIVGQGLGEKVALITDGRFSGGSYGLVVGH
VAPEAAVGGTIGLVEEGDSITVDANKLLIQLNVEERELARRKEKWEKPKPRYKTGILGKYSRLVSSSSQGATTDQI

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=607, Percent_Identity=39.3739703459638, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1788157, Length=516, Percent_Identity=32.5581395348837, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI2367371, Length=494, Percent_Identity=33.4008097165992, Blast_Score=202, Evalue=6e-53,
Organism=Escherichia coli, GI1786464, Length=521, Percent_Identity=31.8618042226488, Blast_Score=189, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6322476, Length=560, Percent_Identity=49.2857142857143, Blast_Score=535, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 58649; Mature: 58649

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00589 PTS_HPR_SER ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSNAITQGIQRSPNRAMLRAVGFDDNDFNKPIIGIANGHSTITPCNMGLMDLANRAES
CCCCHHHHHHHHCCCCHHEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
ALKEAGAMPQTFGTITVSDGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGC
HHHHHCCCCCCCCEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
DKNMPGAMLSMARMNIPSIFVYGGTIKPGKLDGCDLTVVSSFEAVGQLASGKIDKDRLIA
CCCCCHHHHHHHHCCCCEEEEECCEECCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEE
VEKNAIPGPGSCGGMFTANTMSAAIETMGFSLPFSSTMAAVDDEKAESAAESAQVLVNAV
EECCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHH
KNNIRPLDLLTKEAFENAISVIMAVGGSTNSVLHLLAIARTAGVDLTIDDFERIRQTVPV
HCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCE
ICDLKPSGKYVTVDLHKAGGIPQVMKILLDAGMLHGECKTIEGKTIKEVLRDIPSKPKEN
EEEECCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHCCCCCCCC
QDVIRQISNPIYKKGHLAILKGNLASEGSVAKISGVKTPVLTGPARVFESEEECLTAILD
HHHHHHHCCCHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHHHC
NKVKAGDVVVVRYEGPVGGPGMREMLSPTSAIVGQGLGEKVALITDGRFSGGSYGLVVGH
CCCCCCCEEEEEECCCCCCCCHHHHHCHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEC
VAPEAAVGGTIGLVEEGDSITVDANKLLIQLNVEERELARRKEKWEKPKPRYKTGILGKY
CCCCHHCCCEEEEEECCCEEEEECCEEEEEEECHHHHHHHHHHHCCCCCCCCHHCCHHHH
SRLVSSSSQGATTDQI
HHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLRSNAITQGIQRSPNRAMLRAVGFDDNDFNKPIIGIANGHSTITPCNMGLMDLANRAES
CCCCHHHHHHHHCCCCHHEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
ALKEAGAMPQTFGTITVSDGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGC
HHHHHCCCCCCCCEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
DKNMPGAMLSMARMNIPSIFVYGGTIKPGKLDGCDLTVVSSFEAVGQLASGKIDKDRLIA
CCCCCHHHHHHHHCCCCEEEEECCEECCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEE
VEKNAIPGPGSCGGMFTANTMSAAIETMGFSLPFSSTMAAVDDEKAESAAESAQVLVNAV
EECCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHH
KNNIRPLDLLTKEAFENAISVIMAVGGSTNSVLHLLAIARTAGVDLTIDDFERIRQTVPV
HCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCE
ICDLKPSGKYVTVDLHKAGGIPQVMKILLDAGMLHGECKTIEGKTIKEVLRDIPSKPKEN
EEEECCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHCCCCCCCC
QDVIRQISNPIYKKGHLAILKGNLASEGSVAKISGVKTPVLTGPARVFESEEECLTAILD
HHHHHHHCCCHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHHHC
NKVKAGDVVVVRYEGPVGGPGMREMLSPTSAIVGQGLGEKVALITDGRFSGGSYGLVVGH
CCCCCCCEEEEEECCCCCCCCHHHHHCHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEC
VAPEAAVGGTIGLVEEGDSITVDANKLLIQLNVEERELARRKEKWEKPKPRYKTGILGKY
CCCCHHCCCEEEEEECCCEEEEECCEEEEEEECHHHHHHHHHHHCCCCCCCCHHCCHHHH
SRLVSSSSQGATTDQI
HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA