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The map label for this gene is hisS [H]

Identifier: 124025391

GI number: 124025391

Start: 628611

End: 629882

Strand: Direct

Name: hisS [H]

Synonym: NATL1_06821

Alternate gene names: 124025391

Gene position: 628611-629882 (Clockwise)

Preceding gene: 124025390

Following gene: 124025392

Centisome position: 33.71

GC content: 36.4

Gene sequence:

>1272_bases
TTGACCAATCTCAAGAATTTAAGGGGAATGTTGGATCTTTTACCTGCTCAGAGTCAGGGCTGGCAGAAGGTCGAATCAAT
TGCACTTGAACATTTTAGTCGGGCTGGGCTTCAAGAAATTAGAACACCGATTCTTGAACAAACTGAATTGTTTTCAAGGG
GAATTGGCGAAAATACTGATGTTGTGGGCAAAGAAATGTATAGTTTCGACGATAGAGGTGGTCGTTCTTGCACATTGAGG
CCTGAGGGCACAGCCCCTGTCGCGAGGTCAATTATCCAGCATGGATTATTAAATAATGGCCCTCAAAGACTCTGGTATAG
AGGGCCAATGTTTAGATATGAGCGCCCTCAAGCAGGAAGACAAAGGCAGTTCCATCAAATAGGAGTTGAATTTGTAGGAT
TAGCTTCTGTTATGAGTGATGCTGAGGTCATTTCAATAGCTTGGAACTTTTTAAAGGATGTTGGTCTAAATGATTTAACT
TTAGAAATTAATAGTCTTGGAAGTAATGAAGATCGAAATATTTTCAAAGAAGAGTTGAAAGATTGGCTTAATCAGAGATT
TGACTTGTTAGATGAAGATTCTCAGAAAAGAATTAATGTTAATCCCTTGAGAATATTAGATAGTAAAAATAATTCTACAA
AAGAACTTTTATCTGAAGCTCCTTCTTTAAACGACTTTTTATCTAGTGAGAGTAAAACTAGATTTGATTATTTACAGGAG
TTACTCGTTAATCTCAAGATTCCATATAAAATTAATTATAATTTGGTAAGAGGGCTTGATTATTATTCTCATACAGCTTT
CGAAATAACAAGTGATCATTTAGGTTCTCAAGCTACCGTATGCGGAGGAGGACGTTACGATGGATTGATAAGTGAACTTG
GGGGGCCTCAAGCTCCTTCTATAGGTTGGGCTATTGGAATGGAAAGGCTGGTTATTCTAGCTGGAGATAAAATTTTACAG
ACAAAATCTCCGGATGTTTATGTGATTCATAAAGGTAAAAAAGCTGAGCAACTTGCTTTGGAAATTACTTGTCAGTTAAG
GTCATCTAACTTAATTATTGAATTGGACTACTCAGGTTCATCTTTTTCAAAACAATTTAAGCGAGCAGATAAAAGTAGGG
CTAAATGGGCCTTAGTTATAGGTGAGGATGAGGTATCTAAAGGTCAGTTATTAATGAAGAAATTAAGGGATAAACAAAAG
GATGAGGAGAGTAGGGAATATATTTTCTCAAAGGGGGATCTAGATCAGTTAATTAAAAAGTTGATTGCTTAA

Upstream 100 bases:

>100_bases
ACCAATTTGGTTTAGCAAAACTCATAGATTAAATATTATTTTTCAGTTTACGTAAACAATTACTATTAAACAAACAACAA
ATAAGTTTGCTGAAGTTACT

Downstream 100 bases:

>100_bases
AAAAATGATGAATTATTTTAAATGAGCTCCATTAAAATCCAAAAAATATGTTGTATTGGCGCTGGTTATGTAGGTGGTCC
AACCATGTCTGTTATTGCAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTNLKNLRGMLDLLPAQSQGWQKVESIALEHFSRAGLQEIRTPILEQTELFSRGIGENTDVVGKEMYSFDDRGGRSCTLR
PEGTAPVARSIIQHGLLNNGPQRLWYRGPMFRYERPQAGRQRQFHQIGVEFVGLASVMSDAEVISIAWNFLKDVGLNDLT
LEINSLGSNEDRNIFKEELKDWLNQRFDLLDEDSQKRINVNPLRILDSKNNSTKELLSEAPSLNDFLSSESKTRFDYLQE
LLVNLKIPYKINYNLVRGLDYYSHTAFEITSDHLGSQATVCGGGRYDGLISELGGPQAPSIGWAIGMERLVILAGDKILQ
TKSPDVYVIHKGKKAEQLALEITCQLRSSNLIIELDYSGSSFSKQFKRADKSRAKWALVIGEDEVSKGQLLMKKLRDKQK
DEESREYIFSKGDLDQLIKKLIA

Sequences:

>Translated_423_residues
MTNLKNLRGMLDLLPAQSQGWQKVESIALEHFSRAGLQEIRTPILEQTELFSRGIGENTDVVGKEMYSFDDRGGRSCTLR
PEGTAPVARSIIQHGLLNNGPQRLWYRGPMFRYERPQAGRQRQFHQIGVEFVGLASVMSDAEVISIAWNFLKDVGLNDLT
LEINSLGSNEDRNIFKEELKDWLNQRFDLLDEDSQKRINVNPLRILDSKNNSTKELLSEAPSLNDFLSSESKTRFDYLQE
LLVNLKIPYKINYNLVRGLDYYSHTAFEITSDHLGSQATVCGGGRYDGLISELGGPQAPSIGWAIGMERLVILAGDKILQ
TKSPDVYVIHKGKKAEQLALEITCQLRSSNLIIELDYSGSSFSKQFKRADKSRAKWALVIGEDEVSKGQLLMKKLRDKQK
DEESREYIFSKGDLDQLIKKLIA
>Mature_422_residues
TNLKNLRGMLDLLPAQSQGWQKVESIALEHFSRAGLQEIRTPILEQTELFSRGIGENTDVVGKEMYSFDDRGGRSCTLRP
EGTAPVARSIIQHGLLNNGPQRLWYRGPMFRYERPQAGRQRQFHQIGVEFVGLASVMSDAEVISIAWNFLKDVGLNDLTL
EINSLGSNEDRNIFKEELKDWLNQRFDLLDEDSQKRINVNPLRILDSKNNSTKELLSEAPSLNDFLSSESKTRFDYLQEL
LVNLKIPYKINYNLVRGLDYYSHTAFEITSDHLGSQATVCGGGRYDGLISELGGPQAPSIGWAIGMERLVILAGDKILQT
KSPDVYVIHKGKKAEQLALEITCQLRSSNLIIELDYSGSSFSKQFKRADKSRAKWALVIGEDEVSKGQLLMKKLRDKQKD
EESREYIFSKGDLDQLIKKLIA

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=417, Percent_Identity=45.8033573141487, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71993686, Length=452, Percent_Identity=26.3274336283186, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71993693, Length=452, Percent_Identity=26.3274336283186, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6325290, Length=438, Percent_Identity=22.8310502283105, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47877; Mature: 47746

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLKNLRGMLDLLPAQSQGWQKVESIALEHFSRAGLQEIRTPILEQTELFSRGIGENTD
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
VVGKEMYSFDDRGGRSCTLRPEGTAPVARSIIQHGLLNNGPQRLWYRGPMFRYERPQAGR
HHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCHH
QRQFHQIGVEFVGLASVMSDAEVISIAWNFLKDVGLNDLTLEINSLGSNEDRNIFKEELK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
DWLNQRFDLLDEDSQKRINVNPLRILDSKNNSTKELLSEAPSLNDFLSSESKTRFDYLQE
HHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHH
LLVNLKIPYKINYNLVRGLDYYSHTAFEITSDHLGSQATVCGGGRYDGLISELGGPQAPS
HHHHCCCCEEECCHHEECCHHHCCCEEEEEHHHCCCCEEEECCCCHHHHHHHCCCCCCCC
IGWAIGMERLVILAGDKILQTKSPDVYVIHKGKKAEQLALEITCQLRSSNLIIELDYSGS
CHHHHHHHEEEEEECCCEEECCCCCEEEEECCCCHHHEEEEEEEEEECCCEEEEEECCCC
SFSKQFKRADKSRAKWALVIGEDEVSKGQLLMKKLRDKQKDEESREYIFSKGDLDQLIKK
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHH
LIA
HCC
>Mature Secondary Structure 
TNLKNLRGMLDLLPAQSQGWQKVESIALEHFSRAGLQEIRTPILEQTELFSRGIGENTD
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
VVGKEMYSFDDRGGRSCTLRPEGTAPVARSIIQHGLLNNGPQRLWYRGPMFRYERPQAGR
HHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCHH
QRQFHQIGVEFVGLASVMSDAEVISIAWNFLKDVGLNDLTLEINSLGSNEDRNIFKEELK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
DWLNQRFDLLDEDSQKRINVNPLRILDSKNNSTKELLSEAPSLNDFLSSESKTRFDYLQE
HHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHH
LLVNLKIPYKINYNLVRGLDYYSHTAFEITSDHLGSQATVCGGGRYDGLISELGGPQAPS
HHHHCCCCEEECCHHEECCHHHCCCEEEEEHHHCCCCEEEECCCCHHHHHHHCCCCCCCC
IGWAIGMERLVILAGDKILQTKSPDVYVIHKGKKAEQLALEITCQLRSSNLIIELDYSGS
CHHHHHHHEEEEEECCCEEECCCCCEEEEECCCCHHHEEEEEEEEEECCCEEEEEECCCC
SFSKQFKRADKSRAKWALVIGEDEVSKGQLLMKKLRDKQKDEESREYIFSKGDLDQLIKK
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHH
LIA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA