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The map label for this gene is glgB

Identifier: 124025351

GI number: 124025351

Start: 580801

End: 583068

Strand: Reverse

Name: glgB

Synonym: NATL1_06401

Alternate gene names: 124025351

Gene position: 583068-580801 (Counterclockwise)

Preceding gene: 124025352

Following gene: 124025350

Centisome position: 31.27

GC content: 38.67

Gene sequence:

>2268_bases
ATGACCGTCTCTATCCTACTAGATTCTTTGAAAGACGACGGACAAAGACTTTCTGAATGCAGACATGAAAGTCCATTTTC
AATTCTTGGTCCTCAACCATTTAAAGATAAATGGATAATTCGAATATGGATGCCTGAGGCAAGTGAAGTTGAACTGATAA
CACAACAAACTAAAATTAAGCTACAAAATCCCAATCATGAATGGATATTTGAGGGGGTTTTAGAAAAAGATCCAGGTAAT
GACTATCAAATAAAAGTAAATAGAGGAGGAATTGAACATGTTCAACATGATCCTTGGAGTTTCCGAAAAGAGTGGATGGG
TGAAATTGATAGACATCTTTTCGCGGAGGGAAATCACCATCACATATGGCGAAAAATGGGTGCTCACCTCACTGAAATAA
ATAAAAAGCAAGGCGTTATGTTTTGCTTATGGGCGCCTCATGCAAAGAGTGTTTCCGTTATTGGTGATCTCAATTCATGG
GATGGACGCCATCACCCAATGCAAAAACGTCTAGGAGGAATTTGGGAACTATTCATACCTGGTCTAAGTGAGGGAGATTT
ATACAAATATGAAATCAGGACTGAAAAAGGACATTGCTACGAGAAAGCCGACCCTTATGGTTTTCAACATGAAGTAAGAC
CAGCAAAAAGCTCAGTAATATCAAAAATCGACTCATTTCAATGGAATGATCAGTCTTGGATATCCAATCGTGACAATAGA
GATCCTTTAGAGCAACCAATTTCGGTTTATGAAATGCATTTAGGAAGCTGGATGCATGCTTCCTCGGACGATCCATTTAT
CAACTCAAACGGAGAGCATAGAGCCCCTGTTCCAGCTGCAGATATGAAGCCTGGCTCAAGATTGCTTACATATAAAGAAC
TGGCAAATAAAGTCATTCCCTATGTCAAAGAAAGAGGCTTTACTCATATCGAGCTTATGCCAATTTCAGAGCACCCTTTT
GATGGGTCATGGGGGTATCAAGTTACAGGTTGGTATGCGCCTACAAGTAGATTTGGATCACCAGATGAGTTTCGTGCCTT
CGTTGATTCATGTCATAAAGAAGGAATAGGAATCATTCTCGATTGGGTCCCAGGCCACTTCCCTAAAGATCAGCATGGAC
TAGCTTATTTTGATGGCAGCCATCTATACGAGCATTCAGATCCCAGAGTTGGAGAGCATAAAGAATGGGGAACATTAATT
TTCAATTACAGTCGAAATGAAGTTCGTAATTTTCTAGTTGCAAATCTAATTTTCTGGTTTGATCAATTTCATATAGATGG
CATACGTGTTGATGCAGTTGCCTCAATGCTTTACAAAGACTATCTTCGTCCAGAGGGTGAATGGATACCTAATGAAGATG
GAGGGAATGAAAATTTTGAAGCAGTCAGATTTCTACAACAGGCTAATCACGTTCTTTTTCAACATTTTCCAGGTGCACTT
TCTATTGCAGAAGAATCAACGACATGGAGTGGTGTAACCAAACCAACTGACATGGATGGACTCGGTTTCAATCTGAAATG
GAACATGGGTTGGATGCACGATATGCTCGATTATTTTGAAATTGACCCATGGTTTAGACAATTCAATCAAAACAACATTA
CTTTCTCCATTTGTTATAACTTTACCGAAAACTTTATGCTTGCTTTAAGCCACGATGAAGTTGTTCACGGGAAAAGTCAT
CTCTTACACAAAATGCCAGGCGATGACTGGCAGAAGTACGCTAATACACGAGCCTTACTTGCCTATATGTGGACACATCC
AGGTAAAAAAACAATATTTATGGGAATGGAATTTGGGCAGCGCCAAGAATGGAATGTTTGGGATGATTTGCAATGGGATC
TTCTAAATTATGAACCTCATAAAGGAATACAGAAATTAGTAGATGATTTGAATAATTTATATAAAAGAGAACCTGCTTTG
TGGAGAAATGATTTTGACGAATACGGATTCCAGTGGATTGATTGCGATGATAATAAAAATTCAGTAATCAGTTTCATGAG
AAGAGAAAAAACTGATGGAGAATGGCTAGTAATAGTGGCAAACTTCACGCCTCAAAACCACTCTAATTACAGAATAGGTG
TGCCTGTTGACGGATTCTATGAAGAAATTTTCAATACAGATGCGAGTCAATATGGAGGTTCAAACCTAGGTAATATGGGA
GGGAAATCAACAGATCTATACAACATACATGGCTATGAAAATTCTATAGATCTTTGCCTTCCTCCTCTTAGCGTTCTGGT
TCTAAAGCATAAATCCAAGAAGAATTAA

Upstream 100 bases:

>100_bases
TTTTAAATTCAAAACTTAAATTTGAATCACTTATTTCCTCGTAGGGGTTCAATATTTACTTGTAGAGGATTAAACTTACT
AGCTATTTAAGCTATAAGTC

Downstream 100 bases:

>100_bases
GCTTCCTCGAATAGAAATGTTTCAACCTTGACTTAAAACCTAATTTCAACTGTCGTCTGGATGTAAAAATTCAAAAGGTA
TGTCGAACTTTTGCAGAGAA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MTVSILLDSLKDDGQRLSECRHESPFSILGPQPFKDKWIIRIWMPEASEVELITQQTKIKLQNPNHEWIFEGVLEKDPGN
DYQIKVNRGGIEHVQHDPWSFRKEWMGEIDRHLFAEGNHHHIWRKMGAHLTEINKKQGVMFCLWAPHAKSVSVIGDLNSW
DGRHHPMQKRLGGIWELFIPGLSEGDLYKYEIRTEKGHCYEKADPYGFQHEVRPAKSSVISKIDSFQWNDQSWISNRDNR
DPLEQPISVYEMHLGSWMHASSDDPFINSNGEHRAPVPAADMKPGSRLLTYKELANKVIPYVKERGFTHIELMPISEHPF
DGSWGYQVTGWYAPTSRFGSPDEFRAFVDSCHKEGIGIILDWVPGHFPKDQHGLAYFDGSHLYEHSDPRVGEHKEWGTLI
FNYSRNEVRNFLVANLIFWFDQFHIDGIRVDAVASMLYKDYLRPEGEWIPNEDGGNENFEAVRFLQQANHVLFQHFPGAL
SIAEESTTWSGVTKPTDMDGLGFNLKWNMGWMHDMLDYFEIDPWFRQFNQNNITFSICYNFTENFMLALSHDEVVHGKSH
LLHKMPGDDWQKYANTRALLAYMWTHPGKKTIFMGMEFGQRQEWNVWDDLQWDLLNYEPHKGIQKLVDDLNNLYKREPAL
WRNDFDEYGFQWIDCDDNKNSVISFMRREKTDGEWLVIVANFTPQNHSNYRIGVPVDGFYEEIFNTDASQYGGSNLGNMG
GKSTDLYNIHGYENSIDLCLPPLSVLVLKHKSKKN

Sequences:

>Translated_755_residues
MTVSILLDSLKDDGQRLSECRHESPFSILGPQPFKDKWIIRIWMPEASEVELITQQTKIKLQNPNHEWIFEGVLEKDPGN
DYQIKVNRGGIEHVQHDPWSFRKEWMGEIDRHLFAEGNHHHIWRKMGAHLTEINKKQGVMFCLWAPHAKSVSVIGDLNSW
DGRHHPMQKRLGGIWELFIPGLSEGDLYKYEIRTEKGHCYEKADPYGFQHEVRPAKSSVISKIDSFQWNDQSWISNRDNR
DPLEQPISVYEMHLGSWMHASSDDPFINSNGEHRAPVPAADMKPGSRLLTYKELANKVIPYVKERGFTHIELMPISEHPF
DGSWGYQVTGWYAPTSRFGSPDEFRAFVDSCHKEGIGIILDWVPGHFPKDQHGLAYFDGSHLYEHSDPRVGEHKEWGTLI
FNYSRNEVRNFLVANLIFWFDQFHIDGIRVDAVASMLYKDYLRPEGEWIPNEDGGNENFEAVRFLQQANHVLFQHFPGAL
SIAEESTTWSGVTKPTDMDGLGFNLKWNMGWMHDMLDYFEIDPWFRQFNQNNITFSICYNFTENFMLALSHDEVVHGKSH
LLHKMPGDDWQKYANTRALLAYMWTHPGKKTIFMGMEFGQRQEWNVWDDLQWDLLNYEPHKGIQKLVDDLNNLYKREPAL
WRNDFDEYGFQWIDCDDNKNSVISFMRREKTDGEWLVIVANFTPQNHSNYRIGVPVDGFYEEIFNTDASQYGGSNLGNMG
GKSTDLYNIHGYENSIDLCLPPLSVLVLKHKSKKN
>Mature_754_residues
TVSILLDSLKDDGQRLSECRHESPFSILGPQPFKDKWIIRIWMPEASEVELITQQTKIKLQNPNHEWIFEGVLEKDPGND
YQIKVNRGGIEHVQHDPWSFRKEWMGEIDRHLFAEGNHHHIWRKMGAHLTEINKKQGVMFCLWAPHAKSVSVIGDLNSWD
GRHHPMQKRLGGIWELFIPGLSEGDLYKYEIRTEKGHCYEKADPYGFQHEVRPAKSSVISKIDSFQWNDQSWISNRDNRD
PLEQPISVYEMHLGSWMHASSDDPFINSNGEHRAPVPAADMKPGSRLLTYKELANKVIPYVKERGFTHIELMPISEHPFD
GSWGYQVTGWYAPTSRFGSPDEFRAFVDSCHKEGIGIILDWVPGHFPKDQHGLAYFDGSHLYEHSDPRVGEHKEWGTLIF
NYSRNEVRNFLVANLIFWFDQFHIDGIRVDAVASMLYKDYLRPEGEWIPNEDGGNENFEAVRFLQQANHVLFQHFPGALS
IAEESTTWSGVTKPTDMDGLGFNLKWNMGWMHDMLDYFEIDPWFRQFNQNNITFSICYNFTENFMLALSHDEVVHGKSHL
LHKMPGDDWQKYANTRALLAYMWTHPGKKTIFMGMEFGQRQEWNVWDDLQWDLLNYEPHKGIQKLVDDLNNLYKREPALW
RNDFDEYGFQWIDCDDNKNSVISFMRREKTDGEWLVIVANFTPQNHSNYRIGVPVDGFYEEIFNTDASQYGGSNLGNMGG
KSTDLYNIHGYENSIDLCLPPLSVLVLKHKSKKN

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=674, Percent_Identity=25.9643916913947, Blast_Score=196, Evalue=8e-50,
Organism=Escherichia coli, GI1789839, Length=736, Percent_Identity=46.3315217391304, Blast_Score=669, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=639, Percent_Identity=25.3521126760563, Blast_Score=171, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32564391, Length=608, Percent_Identity=25, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320826, Length=696, Percent_Identity=26.2931034482759, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI28573410, Length=717, Percent_Identity=26.4993026499303, Blast_Score=202, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_PROM1 (A2C142)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014467.1
- ProteinModelPortal:   A2C142
- SMR:   A2C142
- STRING:   A2C142
- GeneID:   4779334
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_06401
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- ProtClustDB:   PRK05402
- BioCyc:   PMAR167555:NATL1_06401-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 87954; Mature: 87822

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: ACT_SITE 326-326 ACT_SITE 361-361 ACT_SITE 366-366 ACT_SITE 429-429 ACT_SITE 431-431 ACT_SITE 484-484 ACT_SITE 551-551 ACT_SITE 552-552

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSILLDSLKDDGQRLSECRHESPFSILGPQPFKDKWIIRIWMPEASEVELITQQTKIK
CCHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEEEE
LQNPNHEWIFEGVLEKDPGNDYQIKVNRGGIEHVQHDPWSFRKEWMGEIDRHLFAEGNHH
EECCCCCEEEEEHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCH
HIWRKMGAHLTEINKKQGVMFCLWAPHAKSVSVIGDLNSWDGRHHPMQKRLGGIWELFIP
HHHHHHCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHCCEEEECCC
GLSEGDLYKYEIRTEKGHCYEKADPYGFQHEVRPAKSSVISKIDSFQWNDQSWISNRDNR
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCC
DPLEQPISVYEMHLGSWMHASSDDPFINSNGEHRAPVPAADMKPGSRLLTYKELANKVIP
CHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
YVKERGFTHIELMPISEHPFDGSWGYQVTGWYAPTSRFGSPDEFRAFVDSCHKEGIGIIL
HHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEE
DWVPGHFPKDQHGLAYFDGSHLYEHSDPRVGEHKEWGTLIFNYSRNEVRNFLVANLIFWF
ECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
DQFHIDGIRVDAVASMLYKDYLRPEGEWIPNEDGGNENFEAVRFLQQANHVLFQHFPGAL
HHHEECCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
SIAEESTTWSGVTKPTDMDGLGFNLKWNMGWMHDMLDYFEIDPWFRQFNQNNITFSICYN
EECCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCEEEEEEEC
FTENFMLALSHDEVVHGKSHLLHKMPGDDWQKYANTRALLAYMWTHPGKKTIFMGMEFGQ
CCCCEEEEECCCCHHCCHHHHHHCCCCHHHHHHHCHHEEEEEEECCCCCEEEEEECCCCC
RQEWNVWDDLQWDLLNYEPHKGIQKLVDDLNNLYKREPALWRNDFDEYGFQWIDCDDNKN
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCEEEECCCCHH
SVISFMRREKTDGEWLVIVANFTPQNHSNYRIGVPVDGFYEEIFNTDASQYGGSNLGNMG
HHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHCCCHHHCCCCCCCCCC
GKSTDLYNIHGYENSIDLCLPPLSVLVLKHKSKKN
CCCCEEEEECCCCCCCCEECCCHHHHHHHCCCCCC
>Mature Secondary Structure 
TVSILLDSLKDDGQRLSECRHESPFSILGPQPFKDKWIIRIWMPEASEVELITQQTKIK
CHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEEEE
LQNPNHEWIFEGVLEKDPGNDYQIKVNRGGIEHVQHDPWSFRKEWMGEIDRHLFAEGNHH
EECCCCCEEEEEHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCH
HIWRKMGAHLTEINKKQGVMFCLWAPHAKSVSVIGDLNSWDGRHHPMQKRLGGIWELFIP
HHHHHHCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHCCEEEECCC
GLSEGDLYKYEIRTEKGHCYEKADPYGFQHEVRPAKSSVISKIDSFQWNDQSWISNRDNR
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCC
DPLEQPISVYEMHLGSWMHASSDDPFINSNGEHRAPVPAADMKPGSRLLTYKELANKVIP
CHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
YVKERGFTHIELMPISEHPFDGSWGYQVTGWYAPTSRFGSPDEFRAFVDSCHKEGIGIIL
HHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEE
DWVPGHFPKDQHGLAYFDGSHLYEHSDPRVGEHKEWGTLIFNYSRNEVRNFLVANLIFWF
ECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
DQFHIDGIRVDAVASMLYKDYLRPEGEWIPNEDGGNENFEAVRFLQQANHVLFQHFPGAL
HHHEECCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
SIAEESTTWSGVTKPTDMDGLGFNLKWNMGWMHDMLDYFEIDPWFRQFNQNNITFSICYN
EECCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCEEEEEEEC
FTENFMLALSHDEVVHGKSHLLHKMPGDDWQKYANTRALLAYMWTHPGKKTIFMGMEFGQ
CCCCEEEEECCCCHHCCHHHHHHCCCCHHHHHHHCHHEEEEEEECCCCCEEEEEECCCCC
RQEWNVWDDLQWDLLNYEPHKGIQKLVDDLNNLYKREPALWRNDFDEYGFQWIDCDDNKN
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCEEEECCCCHH
SVISFMRREKTDGEWLVIVANFTPQNHSNYRIGVPVDGFYEEIFNTDASQYGGSNLGNMG
HHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHCCCHHHCCCCCCCCCC
GKSTDLYNIHGYENSIDLCLPPLSVLVLKHKSKKN
CCCCEEEEECCCCCCCCEECCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA