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The map label for this gene is cocE [H]

Identifier: 124025352

GI number: 124025352

Start: 583125

End: 584702

Strand: Reverse

Name: cocE [H]

Synonym: NATL1_06411

Alternate gene names: 124025352

Gene position: 584702-583125 (Counterclockwise)

Preceding gene: 124025353

Following gene: 124025351

Centisome position: 31.36

GC content: 40.56

Gene sequence:

>1578_bases
ATGAATTCAATAAGATATAGGGACGAAGACTTACAACTTAGAGACGGAACAGTACTCAAATCTCGAATCTGGTCGCCTCA
GGGGAATGGACCATGGCCAGTCTTACTTATGCGCCAGCCATATGGAAGAGAAATTGCTTCTACAATTACTTATGCCCATC
CATCTTGGTGGGCTAGCAAGGGCTATCTAGTTGTCATTCAAGATGTTCGTGGTCAAGGTGGGTCTGGAGGGGAATTTTCA
GGTTTCAATCAAGAAGCTTCAGACACTAGTCAAACTCATAATTGGGTCCGATCCTTACCTGAATGCAATGGTCTACTAGG
CACATACGGTTTTTCATATCAAGGATTAACACAGCTCATTGCGGAGGAAGGTACTCCCCCACCTGATTGCATTATTCCTG
CAATGACAGGCCTTTCGGAGGATGAACATTGGAGTTGTGAAGGAGGAGCGTTTTGGTGGCACTTGGGAATTGGATGGGGT
TTGCAATTAGCCGCCCAAAAAGCACAAAGAGAGAAGAATTGGAAAGGTTGGCATGAAATCCGTGAAAATCTTGAATCGAA
AAAATATTTATATAACGGTCATGATTTACTTGAAAAGTATGACCCAGAAGGGATGGCATATAAATGGCTAAATCTCTCGT
CAAGCAAAACTCCTCAATGGAAAACTCATAAGCCATTAAGTAGCTGGTTAAAAAAACCCTTGCTTCTAATTGGGGGTTGG
TGGGATCCTCATTTGAAAGGAATTTTAGATATTTTTGAGAAAGCTAAAAAGGCTGGAGGCAATCCCAAATTACTAATTGG
TCCTGCTACTCATCTTCAATGGTGGGAGGGAGCACAACGCACCCAGTTAGATTTTTTTGATTCTCATTTAAAAGAAAAAA
ACAAAGAGAGTCATTTCTCTCATATCAATCTTTGGAATTTAACCACTAAGAGTTGGGAGCTTTCGGTTCAAAATAAAACT
CCTACTTGGAGTCTTCGGAGCGAAGGTTTAGCCTCAATAAATCATCTTGAAGGATTCCTAGCCCCGAATTCTGATTTAGA
AGCAGATAGTGAGGTGAAATTAGTTCACGACCCATGGAGACCCATACCTTCCATCGGAGGACATCTGAGTGATACCCCTG
GTGAAGCAAACAGATATCAGCTTGATATTCGACCTGATGTCGCAGTATTTACATCAGACTCTATTTCGAAAGATCTTAGG
CTGGAGGGCATTCCAACTCTTTTTTTAGAAACTAACTGTGACAGGGAATGTTTTGATCTGTTTGTTGCATTATCAATAAT
TCCTAAAGCGGTAGAGAATACAGTTACTCAACTTTCGACGGGTGTACTCAGAATTGCTGATTGTGATAAAGGTATAACAA
GTGCTAGAGAAATCAGACTTCAACCTATTCTCGCCACATTTAAAAAAGGGGATCGTTTAAGAATTTCAATTTCAGGATCT
GGATGGCCAGCAATTGGAATAAATCCTGGTCAGAGTAAATATTTGTGCGAAGGACCTAGCCCTAACTGCCTTGTGACAAC
AATTTCACTCTTACTTTTAAATTCAAAACTTAAATTTGAATCACTTATTTCCTCGTAG

Upstream 100 bases:

>100_bases
CAGAAAATCTCAATTCAAAAAAATATAAGCGAAGGAGATGCTGAGATCCTTTCTATGTACGTAAGAGGTCTTTATTGCCC
AGAAACTTTTTAAAGAGATA

Downstream 100 bases:

>100_bases
GGGTTCAATATTTACTTGTAGAGGATTAAACTTACTAGCTATTTAAGCTATAAGTCATGACCGTCTCTATCCTACTAGAT
TCTTTGAAAGACGACGGACA

Product: acyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MNSIRYRDEDLQLRDGTVLKSRIWSPQGNGPWPVLLMRQPYGREIASTITYAHPSWWASKGYLVVIQDVRGQGGSGGEFS
GFNQEASDTSQTHNWVRSLPECNGLLGTYGFSYQGLTQLIAEEGTPPPDCIIPAMTGLSEDEHWSCEGGAFWWHLGIGWG
LQLAAQKAQREKNWKGWHEIRENLESKKYLYNGHDLLEKYDPEGMAYKWLNLSSSKTPQWKTHKPLSSWLKKPLLLIGGW
WDPHLKGILDIFEKAKKAGGNPKLLIGPATHLQWWEGAQRTQLDFFDSHLKEKNKESHFSHINLWNLTTKSWELSVQNKT
PTWSLRSEGLASINHLEGFLAPNSDLEADSEVKLVHDPWRPIPSIGGHLSDTPGEANRYQLDIRPDVAVFTSDSISKDLR
LEGIPTLFLETNCDRECFDLFVALSIIPKAVENTVTQLSTGVLRIADCDKGITSAREIRLQPILATFKKGDRLRISISGS
GWPAIGINPGQSKYLCEGPSPNCLVTTISLLLLNSKLKFESLISS

Sequences:

>Translated_525_residues
MNSIRYRDEDLQLRDGTVLKSRIWSPQGNGPWPVLLMRQPYGREIASTITYAHPSWWASKGYLVVIQDVRGQGGSGGEFS
GFNQEASDTSQTHNWVRSLPECNGLLGTYGFSYQGLTQLIAEEGTPPPDCIIPAMTGLSEDEHWSCEGGAFWWHLGIGWG
LQLAAQKAQREKNWKGWHEIRENLESKKYLYNGHDLLEKYDPEGMAYKWLNLSSSKTPQWKTHKPLSSWLKKPLLLIGGW
WDPHLKGILDIFEKAKKAGGNPKLLIGPATHLQWWEGAQRTQLDFFDSHLKEKNKESHFSHINLWNLTTKSWELSVQNKT
PTWSLRSEGLASINHLEGFLAPNSDLEADSEVKLVHDPWRPIPSIGGHLSDTPGEANRYQLDIRPDVAVFTSDSISKDLR
LEGIPTLFLETNCDRECFDLFVALSIIPKAVENTVTQLSTGVLRIADCDKGITSAREIRLQPILATFKKGDRLRISISGS
GWPAIGINPGQSKYLCEGPSPNCLVTTISLLLLNSKLKFESLISS
>Mature_525_residues
MNSIRYRDEDLQLRDGTVLKSRIWSPQGNGPWPVLLMRQPYGREIASTITYAHPSWWASKGYLVVIQDVRGQGGSGGEFS
GFNQEASDTSQTHNWVRSLPECNGLLGTYGFSYQGLTQLIAEEGTPPPDCIIPAMTGLSEDEHWSCEGGAFWWHLGIGWG
LQLAAQKAQREKNWKGWHEIRENLESKKYLYNGHDLLEKYDPEGMAYKWLNLSSSKTPQWKTHKPLSSWLKKPLLLIGGW
WDPHLKGILDIFEKAKKAGGNPKLLIGPATHLQWWEGAQRTQLDFFDSHLKEKNKESHFSHINLWNLTTKSWELSVQNKT
PTWSLRSEGLASINHLEGFLAPNSDLEADSEVKLVHDPWRPIPSIGGHLSDTPGEANRYQLDIRPDVAVFTSDSISKDLR
LEGIPTLFLETNCDRECFDLFVALSIIPKAVENTVTQLSTGVLRIADCDKGITSAREIRLQPILATFKKGDRLRISISGS
GWPAIGINPGQSKYLCEGPSPNCLVTTISLLLLNSKLKFESLISS

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 58937; Mature: 58937

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIRYRDEDLQLRDGTVLKSRIWSPQGNGPWPVLLMRQPYGREIASTITYAHPSWWASK
CCCCCCCCCCCEECCCCEEHHHCCCCCCCCCCEEEEECCCCCHHHHHHHEECCCCCCCCC
GYLVVIQDVRGQGGSGGEFSGFNQEASDTSQTHNWVRSLPECNGLLGTYGFSYQGLTQLI
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHH
AEEGTPPPDCIIPAMTGLSEDEHWSCEGGAFWWHLGIGWGLQLAAQKAQREKNWKGWHEI
HHCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHH
RENLESKKYLYNGHDLLEKYDPEGMAYKWLNLSSSKTPQWKTHKPLSSWLKKPLLLIGGW
HHHHHHHHEEECCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCEEEEECC
WDPHLKGILDIFEKAKKAGGNPKLLIGPATHLQWWEGAQRTQLDFFDSHLKEKNKESHFS
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCC
HINLWNLTTKSWELSVQNKTPTWSLRSEGLASINHLEGFLAPNSDLEADSEVKLVHDPWR
EEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCC
PIPSIGGHLSDTPGEANRYQLDIRPDVAVFTSDSISKDLRLEGIPTLFLETNCDRECFDL
CCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCEEECCCCEEEEECCCCHHHHHH
FVALSIIPKAVENTVTQLSTGVLRIADCDKGITSAREIRLQPILATFKKGDRLRISISGS
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHEEHHHHHHHHCCCCEEEEEECCC
GWPAIGINPGQSKYLCEGPSPNCLVTTISLLLLNSKLKFESLISS
CCCEECCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MNSIRYRDEDLQLRDGTVLKSRIWSPQGNGPWPVLLMRQPYGREIASTITYAHPSWWASK
CCCCCCCCCCCEECCCCEEHHHCCCCCCCCCCEEEEECCCCCHHHHHHHEECCCCCCCCC
GYLVVIQDVRGQGGSGGEFSGFNQEASDTSQTHNWVRSLPECNGLLGTYGFSYQGLTQLI
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHH
AEEGTPPPDCIIPAMTGLSEDEHWSCEGGAFWWHLGIGWGLQLAAQKAQREKNWKGWHEI
HHCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHH
RENLESKKYLYNGHDLLEKYDPEGMAYKWLNLSSSKTPQWKTHKPLSSWLKKPLLLIGGW
HHHHHHHHEEECCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCEEEEECC
WDPHLKGILDIFEKAKKAGGNPKLLIGPATHLQWWEGAQRTQLDFFDSHLKEKNKESHFS
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCC
HINLWNLTTKSWELSVQNKTPTWSLRSEGLASINHLEGFLAPNSDLEADSEVKLVHDPWR
EEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCC
PIPSIGGHLSDTPGEANRYQLDIRPDVAVFTSDSISKDLRLEGIPTLFLETNCDRECFDL
CCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCEEECCCCEEEEECCCCHHHHHH
FVALSIIPKAVENTVTQLSTGVLRIADCDKGITSAREIRLQPILATFKKGDRLRISISGS
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHEEHHHHHHHHCCCCEEEEEECCC
GWPAIGINPGQSKYLCEGPSPNCLVTTISLLLLNSKLKFESLISS
CCCEECCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]