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The map label for this gene is rpoD4 [H]

Identifier: 124025343

GI number: 124025343

Start: 572599

End: 573522

Strand: Reverse

Name: rpoD4 [H]

Synonym: NATL1_06321

Alternate gene names: 124025343

Gene position: 573522-572599 (Counterclockwise)

Preceding gene: 124025344

Following gene: 124025342

Centisome position: 30.76

GC content: 42.42

Gene sequence:

>924_bases
GTGAGTTCTTTAAGCGACTTTCTTGGAGAAATAGGAAGACACGAATTGCTCACACCAGAGCAAGAACTCACTTTGGGTAG
AGAAGTGCAAGCAATGGTTGCACTTAATGAACGCTGCCAACAAGCTGGAGGTAATGGACCAGAGTGTGAATATTCAAGCG
CAGAGAAGAAAAGATTTAAGGCAGGTGAACGGGCCAAGAATCAAATGATCACAGCAAATCTGAGGCTTGTTGTGAATTTG
GCCAAGCGCTATCAAGGCAAAGGGCTTGACCTTCTTGATTTAATTCAAGAGGGCACCTTAGGATTAACAAGAGCTGTTGA
GAAGTACGATCCCAAAAGAGGTCATCGATTTTCAACTTACGCTTACTGGTGGATTAGGCAAGGACTAAATAGAGCGCTTT
CAACGCAAAGTAGAACAATACGAATCCCTGTAAACATAAATGAAAAACTAACAAAACTTCGTGCTGCCAAGTCTCGACTA
ATGCAAGAGCTTGGGGTTCATCCCTCAACTAACCAGATAGCCATACAAATGAAAATTCCTTTAGAGGAAGTAGAAGAATT
ACTTGCATGTGAGCTAAGAAGTATCACAGTGAGCTTGCAAGGGGCAGTCAAATCTAAAGCAGATCCCTCTGAGCTTGTCG
ATATTCTTCCAAGTGAAGAAGTACCTCCCATGGAACTAGCTGAATTAGCAGAGAGAAGTGCATCAGCCTGGTCGTTATTG
GACAAATCAAATCTCACACCAAAAGAGAGAACGATACTAAGCCTGCGATTTGGCCTAGATGGATCGAACGAATGGAGAAC
TCTAGCCGAAGTCGCCAGACAAATGAATTGCAGCAGGGAATATTGCAGACAAGTAGTTCAACGTGCATTAAGAAAGTTGA
GAAAAACAGGGATTCAAAGTGGTCTTTTAGAGACAAGTATTTAA

Upstream 100 bases:

>100_bases
GAATAGATAAAAGGATTACTAATAAATTCCGAACACTTTATTACGCCTATTGAAAGTAAACAATTTTTCTCTAGGTTATA
AATATAAGGAGGAACAAATC

Downstream 100 bases:

>100_bases
TTGTGGTTAGTTCAAGAAACTCCAATAAAGAAGTTTTTGAAGCAGAGGTACTTGAAAGTTCTGTTATCGACGAGGGGGTA
CTTAAAAAGATCTTATTAAG

Product: type II alternative sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSSLSDFLGEIGRHELLTPEQELTLGREVQAMVALNERCQQAGGNGPECEYSSAEKKRFKAGERAKNQMITANLRLVVNL
AKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPKRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNINEKLTKLRAAKSRL
MQELGVHPSTNQIAIQMKIPLEEVEELLACELRSITVSLQGAVKSKADPSELVDILPSEEVPPMELAELAERSASAWSLL
DKSNLTPKERTILSLRFGLDGSNEWRTLAEVARQMNCSREYCRQVVQRALRKLRKTGIQSGLLETSI

Sequences:

>Translated_307_residues
MSSLSDFLGEIGRHELLTPEQELTLGREVQAMVALNERCQQAGGNGPECEYSSAEKKRFKAGERAKNQMITANLRLVVNL
AKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPKRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNINEKLTKLRAAKSRL
MQELGVHPSTNQIAIQMKIPLEEVEELLACELRSITVSLQGAVKSKADPSELVDILPSEEVPPMELAELAERSASAWSLL
DKSNLTPKERTILSLRFGLDGSNEWRTLAEVARQMNCSREYCRQVVQRALRKLRKTGIQSGLLETSI
>Mature_306_residues
SSLSDFLGEIGRHELLTPEQELTLGREVQAMVALNERCQQAGGNGPECEYSSAEKKRFKAGERAKNQMITANLRLVVNLA
KRYQGKGLDLLDLIQEGTLGLTRAVEKYDPKRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNINEKLTKLRAAKSRLM
QELGVHPSTNQIAIQMKIPLEEVEELLACELRSITVSLQGAVKSKADPSELVDILPSEEVPPMELAELAERSASAWSLLD
KSNLTPKERTILSLRFGLDGSNEWRTLAEVARQMNCSREYCRQVVQRALRKLRKTGIQSGLLETSI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=243, Percent_Identity=39.0946502057613, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1789098, Length=299, Percent_Identity=38.4615384615385, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789871, Length=253, Percent_Identity=28.4584980237154, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34545; Mature: 34414

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSDFLGEIGRHELLTPEQELTLGREVQAMVALNERCQQAGGNGPECEYSSAEKKRFK
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AGERAKNQMITANLRLVVNLAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPKRGHRFSTY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHH
AYWWIRQGLNRALSTQSRTIRIPVNINEKLTKLRAAKSRLMQELGVHPSTNQIAIQMKIP
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
LEEVEELLACELRSITVSLQGAVKSKADPSELVDILPSEEVPPMELAELAERSASAWSLL
HHHHHHHHHHHHHHHHEEEHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHH
DKSNLTPKERTILSLRFGLDGSNEWRTLAEVARQMNCSREYCRQVVQRALRKLRKTGIQS
HCCCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GLLETSI
CHHHCCC
>Mature Secondary Structure 
SSLSDFLGEIGRHELLTPEQELTLGREVQAMVALNERCQQAGGNGPECEYSSAEKKRFK
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AGERAKNQMITANLRLVVNLAKRYQGKGLDLLDLIQEGTLGLTRAVEKYDPKRGHRFSTY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHH
AYWWIRQGLNRALSTQSRTIRIPVNINEKLTKLRAAKSRLMQELGVHPSTNQIAIQMKIP
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
LEEVEELLACELRSITVSLQGAVKSKADPSELVDILPSEEVPPMELAELAERSASAWSLL
HHHHHHHHHHHHHHHHEEEHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHH
DKSNLTPKERTILSLRFGLDGSNEWRTLAEVARQMNCSREYCRQVVQRALRKLRKTGIQS
HCCCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GLLETSI
CHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA