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The map label for this gene is phrB [H]

Identifier: 124025078

GI number: 124025078

Start: 337695

End: 339209

Strand: Reverse

Name: phrB [H]

Synonym: NATL1_03651

Alternate gene names: 124025078

Gene position: 339209-337695 (Counterclockwise)

Preceding gene: 124025079

Following gene: 124025075

Centisome position: 18.19

GC content: 32.48

Gene sequence:

>1515_bases
ATGGTTCAGAGCATGCCAACTCTTGAATATAAGATGACAAAATTTCAATCAATATTTTGGCATAGGAGAGATTTAAGATT
TGGAGATAATATCGGCCTATTCGAAGCATCAAAAAATTCAAAAAGCCTCATAGGAGTATATGTTTTAGATCCCAACCTCT
TAGATCTAAATAGAACTACATCTGAAGCAAAAAACTGGTTTTTAGGTGAAAGTCTTTTAGAACTACAAAAGAATTGGGAA
ATTAGAGGAAGTCTTTTATTAATATTAAATGGAGATCCTATTGAATTAATATCTAAATTAGCTGAGTTAGTTCATGCTGA
ATGTATTTACTGGAACGAGAATATTGAACCTTATGAAATTAATAGAGACAAACAAATTGCAGAAAAACTTTCAAAAGAAA
AAAGGAAAGTTTATACATTTCTAGATCAATTAATTGTTAACCCAAGTGATATCAAAACAAACAACGATGAACCATACAAG
GTATATGGACCTTTTTATCGCAAATGGATTGATATAATCAATAGAACCAAATCATCAGATAATAATTTAATACAAACCTC
AGAGACAGCTAAAAAACTTACAGGCCTGAATGAAAGAGAATTATCATCAATTAAAAACTCTGATTTAAACTACTGCATTA
CAAAAAAAAGCAAATCTATTTACGAATTACTTTCTTCAAATAGATTCAGCAATACCAGTCTATGTCCTTGCAAGCCGGGA
GAATCGGAATCAATAAAACAATTAAACTCTTTTATACATTCAGGAGTTATAAATTCATACAATCAAGCAAGAGATATTCC
ATCTTTAGAGAATACTTCTAATCTAAGTGCTGCTTTAAGTTTAGGCACTATAAGTTGTAGAGCAGTATGGAATGGGGCTC
AAGTATCAAAAAGTTCGACACATGACGAATATAAAATAAATTCTATTGATACATGGATAAAGGAACTTGCTTGGAGAGAG
TTTTATCAAAATGCTCTCATTAATTTTCCAGAACTTGAGAAAGGTCCATATAGGGAGAAATGGTTAAATTTTCCATGGCA
AAATAGACCTGATTGGTTTGAAGCGTGGGGAGATGGATTGACTGGTATCCCAATAATTGATGCTGCAATGAGACAACTTA
AGTGTTCTGGATGGATGCATAATCGTTGCAGAATGATTGTTGCCTCTTTTCTAGTAAAAGACCTTTTAATTGATTGGAGA
TTGGGAGAACTTTTCTTCATGAAAAGTTTAGTTGATGGTGACTTGGCAGCAAACAACGGAGGTTGGCAATGGAGTGCTAG
CAGCGGAATGGATCCAAAACCTATGAGAATATTTAATCCTTTTAGACAAGCTTCTAAGTTCGACGAAGATGGTGAATATA
TCCGAAAATGGATTCCTGAGTTATCACATATTTCAACACCTAATTTACTTTCAGGTGAAATAAGTTCTGCGGAGAGAAAT
AGCTATCCCAACCCAATAATAAATCACAAAAATCAAACATCAATCTTCAAAGAATTATATTCGAATATTAAATAA

Upstream 100 bases:

>100_bases
ATGCAGATGAAGATATTGAAGTATTAAAAATTTCTCCAAGAAAGTTAAATGAAATCATTGCAAGTGGGGAAGAGTCACTT
GATGGGAAAACTATTACAGC

Downstream 100 bases:

>100_bases
CTTATTTTTTAAATAGTTCTTTTATTTTATCAATGACATATGATTGCTGAATAGATTTTAATTCTGGGAAAATAGGTAAA
CTAATAACCTGACTACATAC

Product: putative DNA photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MVQSMPTLEYKMTKFQSIFWHRRDLRFGDNIGLFEASKNSKSLIGVYVLDPNLLDLNRTTSEAKNWFLGESLLELQKNWE
IRGSLLLILNGDPIELISKLAELVHAECIYWNENIEPYEINRDKQIAEKLSKEKRKVYTFLDQLIVNPSDIKTNNDEPYK
VYGPFYRKWIDIINRTKSSDNNLIQTSETAKKLTGLNERELSSIKNSDLNYCITKKSKSIYELLSSNRFSNTSLCPCKPG
ESESIKQLNSFIHSGVINSYNQARDIPSLENTSNLSAALSLGTISCRAVWNGAQVSKSSTHDEYKINSIDTWIKELAWRE
FYQNALINFPELEKGPYREKWLNFPWQNRPDWFEAWGDGLTGIPIIDAAMRQLKCSGWMHNRCRMIVASFLVKDLLIDWR
LGELFFMKSLVDGDLAANNGGWQWSASSGMDPKPMRIFNPFRQASKFDEDGEYIRKWIPELSHISTPNLLSGEISSAERN
SYPNPIINHKNQTSIFKELYSNIK

Sequences:

>Translated_504_residues
MVQSMPTLEYKMTKFQSIFWHRRDLRFGDNIGLFEASKNSKSLIGVYVLDPNLLDLNRTTSEAKNWFLGESLLELQKNWE
IRGSLLLILNGDPIELISKLAELVHAECIYWNENIEPYEINRDKQIAEKLSKEKRKVYTFLDQLIVNPSDIKTNNDEPYK
VYGPFYRKWIDIINRTKSSDNNLIQTSETAKKLTGLNERELSSIKNSDLNYCITKKSKSIYELLSSNRFSNTSLCPCKPG
ESESIKQLNSFIHSGVINSYNQARDIPSLENTSNLSAALSLGTISCRAVWNGAQVSKSSTHDEYKINSIDTWIKELAWRE
FYQNALINFPELEKGPYREKWLNFPWQNRPDWFEAWGDGLTGIPIIDAAMRQLKCSGWMHNRCRMIVASFLVKDLLIDWR
LGELFFMKSLVDGDLAANNGGWQWSASSGMDPKPMRIFNPFRQASKFDEDGEYIRKWIPELSHISTPNLLSGEISSAERN
SYPNPIINHKNQTSIFKELYSNIK
>Mature_504_residues
MVQSMPTLEYKMTKFQSIFWHRRDLRFGDNIGLFEASKNSKSLIGVYVLDPNLLDLNRTTSEAKNWFLGESLLELQKNWE
IRGSLLLILNGDPIELISKLAELVHAECIYWNENIEPYEINRDKQIAEKLSKEKRKVYTFLDQLIVNPSDIKTNNDEPYK
VYGPFYRKWIDIINRTKSSDNNLIQTSETAKKLTGLNERELSSIKNSDLNYCITKKSKSIYELLSSNRFSNTSLCPCKPG
ESESIKQLNSFIHSGVINSYNQARDIPSLENTSNLSAALSLGTISCRAVWNGAQVSKSSTHDEYKINSIDTWIKELAWRE
FYQNALINFPELEKGPYREKWLNFPWQNRPDWFEAWGDGLTGIPIIDAAMRQLKCSGWMHNRCRMIVASFLVKDLLIDWR
LGELFFMKSLVDGDLAANNGGWQWSASSGMDPKPMRIFNPFRQASKFDEDGEYIRKWIPELSHISTPNLLSGEISSAERN
SYPNPIINHKNQTSIFKELYSNIK

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=525, Percent_Identity=25.9047619047619, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4758072, Length=509, Percent_Identity=24.5579567779961, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI188536103, Length=469, Percent_Identity=25.3731343283582, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1786926, Length=490, Percent_Identity=35.3061224489796, Blast_Score=260, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324962, Length=507, Percent_Identity=32.3471400394477, Blast_Score=213, Evalue=7e-56,
Organism=Drosophila melanogaster, GI17137248, Length=458, Percent_Identity=27.9475982532751, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24585455, Length=458, Percent_Identity=27.9475982532751, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24648152, Length=498, Percent_Identity=24.6987951807229, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 58181; Mature: 58181

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQSMPTLEYKMTKFQSIFWHRRDLRFGDNIGLFEASKNSKSLIGVYVLDPNLLDLNRTT
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEEEEECCCEEECCCCH
SEAKNWFLGESLLELQKNWEIRGSLLLILNGDPIELISKLAELVHAECIYWNENIEPYEI
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEECCCCCCEEC
NRDKQIAEKLSKEKRKVYTFLDQLIVNPSDIKTNNDEPYKVYGPFYRKWIDIINRTKSSD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCC
NNLIQTSETAKKLTGLNERELSSIKNSDLNYCITKKSKSIYELLSSNRFSNTSLCPCKPG
CCEEECHHHHHHHHCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCC
ESESIKQLNSFIHSGVINSYNQARDIPSLENTSNLSAALSLGTISCRAVWNGAQVSKSST
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCEEEEEEECCCCCCCCCC
HDEYKINSIDTWIKELAWREFYQNALINFPELEKGPYREKWLNFPWQNRPDWFEAWGDGL
CCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHCCCC
TGIPIIDAAMRQLKCSGWMHNRCRMIVASFLVKDLLIDWRLGELFFMKSLVDGDLAANNG
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GWQWSASSGMDPKPMRIFNPFRQASKFDEDGEYIRKWIPELSHISTPNLLSGEISSAERN
CEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHCC
SYPNPIINHKNQTSIFKELYSNIK
CCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVQSMPTLEYKMTKFQSIFWHRRDLRFGDNIGLFEASKNSKSLIGVYVLDPNLLDLNRTT
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEEEEECCCEEECCCCH
SEAKNWFLGESLLELQKNWEIRGSLLLILNGDPIELISKLAELVHAECIYWNENIEPYEI
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEECCCCCCEEC
NRDKQIAEKLSKEKRKVYTFLDQLIVNPSDIKTNNDEPYKVYGPFYRKWIDIINRTKSSD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCC
NNLIQTSETAKKLTGLNERELSSIKNSDLNYCITKKSKSIYELLSSNRFSNTSLCPCKPG
CCEEECHHHHHHHHCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCC
ESESIKQLNSFIHSGVINSYNQARDIPSLENTSNLSAALSLGTISCRAVWNGAQVSKSST
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCEEEEEEECCCCCCCCCC
HDEYKINSIDTWIKELAWREFYQNALINFPELEKGPYREKWLNFPWQNRPDWFEAWGDGL
CCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHCCCC
TGIPIIDAAMRQLKCSGWMHNRCRMIVASFLVKDLLIDWRLGELFFMKSLVDGDLAANNG
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GWQWSASSGMDPKPMRIFNPFRQASKFDEDGEYIRKWIPELSHISTPNLLSGEISSAERN
CEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHCC
SYPNPIINHKNQTSIFKELYSNIK
CCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2837735; 2110564; 9360600 [H]