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The map label for this gene is nudF [H]

Identifier: 124025079

GI number: 124025079

Start: 339173

End: 339733

Strand: Reverse

Name: nudF [H]

Synonym: NATL1_03661

Alternate gene names: 124025079

Gene position: 339733-339173 (Counterclockwise)

Preceding gene: 124025080

Following gene: 124025078

Centisome position: 18.22

GC content: 37.61

Gene sequence:

>561_bases
ATGGCAATTCCAGGACCTGAACCCTCAGAAATTATTGAGACAAAAGCTTGTCTTGATGCAAAAAAAATTCGTTTTGAAAT
TAATAAATTTCTTTTACCTAATTCAATGAAGGGTGAATTTGGGATCATTCGTCATCCTGGGGCTGCATTAGCCGTACCAA
TAACAGATTCTGGAGAAGTCGTTATTCTTCGTCAATACAGATTTGCTTGCTCGAGAAGAATTCTTGAGTTTCCTGCTGGA
ACATTAGAATCAGGCGAAAGCCCCCTTGAATCAATCAAAAGAGAAGTTCAAGAAGAAAGTGGTTACTCTGCAAAAAGATG
GGACAAACTAGGAGAAATGCTTCCTTGCCCAGGATATTCTGACGAAACAATTCATCTTTTCCTTGCAAGAGATCTAACAA
AACTTGAAAAAAAACCTGAGGGCGATGCAGATGAAGATATTGAAGTATTAAAAATTTCTCCAAGAAAGTTAAATGAAATC
ATTGCAAGTGGGGAAGAGTCACTTGATGGGAAAACTATTACAGCATGGTTCAGAGCATGCCAACTCTTGAATATAAGATG
A

Upstream 100 bases:

>100_bases
GAATCAGTTCTCAAAATATTTGGCCTGAATAAAACTCGTAACCAAAATCACCAACCTTTTCTAAAAAAATCAGCCATCCT
ATAAGGATAGAAAAAAAAGC

Downstream 100 bases:

>100_bases
CAAAATTTCAATCAATATTTTGGCATAGGAGAGATTTAAGATTTGGAGATAATATCGGCCTATTCGAAGCATCAAAAAAT
TCAAAAAGCCTCATAGGAGT

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MAIPGPEPSEIIETKACLDAKKIRFEINKFLLPNSMKGEFGIIRHPGAALAVPITDSGEVVILRQYRFACSRRILEFPAG
TLESGESPLESIKREVQEESGYSAKRWDKLGEMLPCPGYSDETIHLFLARDLTKLEKKPEGDADEDIEVLKISPRKLNEI
IASGEESLDGKTITAWFRACQLLNIR

Sequences:

>Translated_186_residues
MAIPGPEPSEIIETKACLDAKKIRFEINKFLLPNSMKGEFGIIRHPGAALAVPITDSGEVVILRQYRFACSRRILEFPAG
TLESGESPLESIKREVQEESGYSAKRWDKLGEMLPCPGYSDETIHLFLARDLTKLEKKPEGDADEDIEVLKISPRKLNEI
IASGEESLDGKTITAWFRACQLLNIR
>Mature_185_residues
AIPGPEPSEIIETKACLDAKKIRFEINKFLLPNSMKGEFGIIRHPGAALAVPITDSGEVVILRQYRFACSRRILEFPAGT
LESGESPLESIKREVQEESGYSAKRWDKLGEMLPCPGYSDETIHLFLARDLTKLEKKPEGDADEDIEVLKISPRKLNEII
ASGEESLDGKTITAWFRACQLLNIR

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788810, Length=137, Percent_Identity=35.7664233576642, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20801; Mature: 20670

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPGPEPSEIIETKACLDAKKIRFEINKFLLPNSMKGEFGIIRHPGAALAVPITDSGEV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEECCCCCE
VILRQYRFACSRRILEFPAGTLESGESPLESIKREVQEESGYSAKRWDKLGEMLPCPGYS
EEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
DETIHLFLARDLTKLEKKPEGDADEDIEVLKISPRKLNEIIASGEESLDGKTITAWFRAC
CCEEEEEEHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHH
QLLNIR
HHHCCC
>Mature Secondary Structure 
AIPGPEPSEIIETKACLDAKKIRFEINKFLLPNSMKGEFGIIRHPGAALAVPITDSGEV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEECCCCCE
VILRQYRFACSRRILEFPAGTLESGESPLESIKREVQEESGYSAKRWDKLGEMLPCPGYS
EEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
DETIHLFLARDLTKLEKKPEGDADEDIEVLKISPRKLNEIIASGEESLDGKTITAWFRAC
CCEEEEEEHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHH
QLLNIR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]