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The map label for this gene is gatD [H]

Identifier: 124027801

GI number: 124027801

Start: 933516

End: 934889

Strand: Reverse

Name: gatD [H]

Synonym: Hbut_0928

Alternate gene names: 124027801

Gene position: 934889-933516 (Counterclockwise)

Preceding gene: 124027803

Following gene: 124027800

Centisome position: 56.08

GC content: 52.98

Gene sequence:

>1374_bases
TTGGAGACTGTTCCAGGCTACACTGGTATTGCCAGGGCTTTGCTTGAAAGAGCTGGTGCAAGGGTTGGTGATAGGATAAG
GATTGTGCAACGTGATGGCAGGGTTTTCGAGGGCATACTGATGCCACGCTACGCGCTTAGTGCCAAGCCAATTATAGTCA
TTAAGCTGGATAACGGCTATAATATCGGTGTTAGGGTCGACGAGCACACCGAGATAACTGTTGTGAAGACACGCGAGGCC
ATATTGAGGGAGGCCGCTGCCGGCGCTCCAGCCGTGGTTCCTCTCCTGGAGGAGCAACCACCGCTAGAGAAGAGGCCACG
AGTTATGATCATCGGTACTGGGGGCACTATTGCTAGTAAGATTGAGTACGAGACCGGCGCTGTTAAGCCTGCAATGTCTG
CACGTGATCTTGTAGAGGCTGTTCCCGAGATCGGGTTTATAGCCGAAGTTTCAGCCGAGGTTCTGATGAATATTCTAAGC
GAGAACATGAGACCAGAGTACTGGGAACGTATAGTGGATGCCGTTGCCAAGAGTATTGCTGAGGGTTTTGAGGGCATCGT
AGTAGCACACGGAACCGATACAATGGCTTATACAGCTTCTGCTCTAGCATTTGCGTTTAGAGGGCTTCCAGTGCCTATTG
CCTTCGTGGGTGCCCAGAGGAGTAGTGATAGGCCCAGCAGCGATGCCTCCTTCAACCTCATATCGGCTGTGCTCGTAGCA
GCCAGGGCCCCCTTTGCCGAGGTTACCGTGGTTATGCACGGAGAGACCGGCGACACCTACGCCTTAGCCCATAGAGCCAC
GAAGGTTAGAAAGATGCATACAAGCCGGCGTGACGCCTTCCAGAGTATTAATGCTAGGCCACTAGCAAGGGTTTACCCGT
TTGAGAGGAGGATAGAACTCGTCTCAGAGCCCCTGCAACGCAGAGGCGAACAAGAGCTAAGAGTTATGAATGGGTTTGAG
CGCAAGGTTGCACTGGTAAAGTATTATCCCGGAATGGATTCGGAGATCATAGACTTTCTCGTGGATCGCGGCTACCGAGG
CATAGTCCTCGAAGGTACTGGGCTTGGCCACGTGGGCGAATGGCTCGTGGACAGTATTAGGAGGGCTGTGGAGAACGAAA
TTGTAGTCGTCATGACTTCACAAACTCTCTTCGGCAGGGTTAACATGAACGTCTACGCTACCGGCCGCAAGTTACTAGCT
GCAGGTGTCGTGCCAGGTGACGATATGCTCCCCGAGACAGCCTTCGTCAAGCTTTCATGGGTTCTAGCGCACTCCTCGAG
TTTTGAGGAGGCAAAGAGACTCATGCGCGAGAACCTAGCTGGAGAGATCAATCCGAGACACACCGTAGACCTATACCCGA
GGTGGCCGCATTGA

Upstream 100 bases:

>100_bases
AACCGTGGCTTGGCATAGTGTTCCCCCAGAACGTTGCTAGAGTGGCGTGGAGTCCTTATATAGGCTTTTATTCTTGGCTT
TTGCTGGGGCTAGGTCTGCC

Downstream 100 bases:

>100_bases
TCGAGCCCAAGGTAGACCCTTCAAAGTATGACCCTCGGGAGCTGGGGCTAAAGGCTGGCCTTGAGATACACCAACAGCTA
AATACTAGAGCCAAGCTGTT

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
METVPGYTGIARALLERAGARVGDRIRIVQRDGRVFEGILMPRYALSAKPIIVIKLDNGYNIGVRVDEHTEITVVKTREA
ILREAAAGAPAVVPLLEEQPPLEKRPRVMIIGTGGTIASKIEYETGAVKPAMSARDLVEAVPEIGFIAEVSAEVLMNILS
ENMRPEYWERIVDAVAKSIAEGFEGIVVAHGTDTMAYTASALAFAFRGLPVPIAFVGAQRSSDRPSSDASFNLISAVLVA
ARAPFAEVTVVMHGETGDTYALAHRATKVRKMHTSRRDAFQSINARPLARVYPFERRIELVSEPLQRRGEQELRVMNGFE
RKVALVKYYPGMDSEIIDFLVDRGYRGIVLEGTGLGHVGEWLVDSIRRAVENEIVVVMTSQTLFGRVNMNVYATGRKLLA
AGVVPGDDMLPETAFVKLSWVLAHSSSFEEAKRLMRENLAGEINPRHTVDLYPRWPH

Sequences:

>Translated_457_residues
METVPGYTGIARALLERAGARVGDRIRIVQRDGRVFEGILMPRYALSAKPIIVIKLDNGYNIGVRVDEHTEITVVKTREA
ILREAAAGAPAVVPLLEEQPPLEKRPRVMIIGTGGTIASKIEYETGAVKPAMSARDLVEAVPEIGFIAEVSAEVLMNILS
ENMRPEYWERIVDAVAKSIAEGFEGIVVAHGTDTMAYTASALAFAFRGLPVPIAFVGAQRSSDRPSSDASFNLISAVLVA
ARAPFAEVTVVMHGETGDTYALAHRATKVRKMHTSRRDAFQSINARPLARVYPFERRIELVSEPLQRRGEQELRVMNGFE
RKVALVKYYPGMDSEIIDFLVDRGYRGIVLEGTGLGHVGEWLVDSIRRAVENEIVVVMTSQTLFGRVNMNVYATGRKLLA
AGVVPGDDMLPETAFVKLSWVLAHSSSFEEAKRLMRENLAGEINPRHTVDLYPRWPH
>Mature_457_residues
METVPGYTGIARALLERAGARVGDRIRIVQRDGRVFEGILMPRYALSAKPIIVIKLDNGYNIGVRVDEHTEITVVKTREA
ILREAAAGAPAVVPLLEEQPPLEKRPRVMIIGTGGTIASKIEYETGAVKPAMSARDLVEAVPEIGFIAEVSAEVLMNILS
ENMRPEYWERIVDAVAKSIAEGFEGIVVAHGTDTMAYTASALAFAFRGLPVPIAFVGAQRSSDRPSSDASFNLISAVLVA
ARAPFAEVTVVMHGETGDTYALAHRATKVRKMHTSRRDAFQSINARPLARVYPFERRIELVSEPLQRRGEQELRVMNGFE
RKVALVKYYPGMDSEIIDFLVDRGYRGIVLEGTGLGHVGEWLVDSIRRAVENEIVVVMTSQTLFGRVNMNVYATGRKLLA
AGVVPGDDMLPETAFVKLSWVLAHSSSFEEAKRLMRENLAGEINPRHTVDLYPRWPH

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=290, Percent_Identity=31.3793103448276, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1788065, Length=288, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789327, Length=325, Percent_Identity=28.3076923076923, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI193207374, Length=395, Percent_Identity=28.3544303797468, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI193207372, Length=389, Percent_Identity=28.5347043701799, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17558278, Length=395, Percent_Identity=28.3544303797468, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17558280, Length=395, Percent_Identity=28.3544303797468, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6320527, Length=342, Percent_Identity=26.9005847953216, Blast_Score=109, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323189, Length=280, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323187, Length=280, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323186, Length=280, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323184, Length=280, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24645530, Length=372, Percent_Identity=31.1827956989247, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24639609, Length=289, Percent_Identity=30.1038062283737, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: 3.5.1.1

Molecular weight: Translated: 50415; Mature: 50415

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVPGYTGIARALLERAGARVGDRIRIVQRDGRVFEGILMPRYALSAKPIIVIKLDNGY
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHCCHHHCCCCCEEEEEECCCC
NIGVRVDEHTEITVVKTREAILREAAAGAPAVVPLLEEQPPLEKRPRVMIIGTGGTIASK
EEEEEECCCCEEEEEECHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCEEEE
IEYETGAVKPAMSARDLVEAVPEIGFIAEVSAEVLMNILSENMRPEYWERIVDAVAKSIA
EEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EGFEGIVVAHGTDTMAYTASALAFAFRGLPVPIAFVGAQRSSDRPSSDASFNLISAVLVA
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
ARAPFAEVTVVMHGETGDTYALAHRATKVRKMHTSRRDAFQSINARPLARVYPFERRIEL
HCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHH
VSEPLQRRGEQELRVMNGFERKVALVKYYPGMDSEIIDFLVDRGYRGIVLEGTGLGHVGE
HHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHHHH
WLVDSIRRAVENEIVVVMTSQTLFGRVNMNVYATGRKLLAAGVVPGDDMLPETAFVKLSW
HHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCCHHEEEEE
VLAHSSSFEEAKRLMRENLAGEINPRHTVDLYPRWPH
EEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
METVPGYTGIARALLERAGARVGDRIRIVQRDGRVFEGILMPRYALSAKPIIVIKLDNGY
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHCCHHHCCCCCEEEEEECCCC
NIGVRVDEHTEITVVKTREAILREAAAGAPAVVPLLEEQPPLEKRPRVMIIGTGGTIASK
EEEEEECCCCEEEEEECHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCEEEE
IEYETGAVKPAMSARDLVEAVPEIGFIAEVSAEVLMNILSENMRPEYWERIVDAVAKSIA
EEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EGFEGIVVAHGTDTMAYTASALAFAFRGLPVPIAFVGAQRSSDRPSSDASFNLISAVLVA
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
ARAPFAEVTVVMHGETGDTYALAHRATKVRKMHTSRRDAFQSINARPLARVYPFERRIEL
HCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHH
VSEPLQRRGEQELRVMNGFERKVALVKYYPGMDSEIIDFLVDRGYRGIVLEGTGLGHVGE
HHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHHHH
WLVDSIRRAVENEIVVVMTSQTLFGRVNMNVYATGRKLLAAGVVPGDDMLPETAFVKLSW
HHHHHHHHHHCCCEEEEEECCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCCHHEEEEE
VLAHSSSFEEAKRLMRENLAGEINPRHTVDLYPRWPH
EEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]