Click here to switch to the map view.

The map label for this gene is gatE [H]

Identifier: 124027800

GI number: 124027800

Start: 931570

End: 933519

Strand: Reverse

Name: gatE [H]

Synonym: Hbut_0927

Alternate gene names: 124027800

Gene position: 933519-931570 (Counterclockwise)

Preceding gene: 124027801

Following gene: 124027798

Centisome position: 55.99

GC content: 51.9

Gene sequence:

>1950_bases
TTGATCGAGCCCAAGGTAGACCCTTCAAAGTATGACCCTCGGGAGCTGGGGCTAAAGGCTGGCCTTGAGATACACCAACA
GCTAAATACTAGAGCCAAGCTGTTCTGCAATTGCCCCACAAGGCTCTCCAGCGAGGACGAGCCAGGAGACGAGTTTGTTC
GGCAACTAAGGCCTACCCGTAGCGAGCTAGGCGAGGTAGACATTGCTGCACTGTTCGAATGGCGTAAGGGTAGAATGTAC
CACTACCATGCGCCAAGGGCTACTAGCTGCCTAGTAGAGGCTGACGAGGAACCTCCACATGAGATTAACCAGGAGGCAGT
GGTTATAGGGCTCGCTGTAGCCCTGGCTCTTGGCGCTACACCCGTAGACGAGATACATGTCATGAGGAAGATTGTCATCG
ATGGCTCAAATACTACAGGGTTCCAGCGCACCGCAATAATAGCCCTGGGAGGCTCGATAGAACTCGAGTCGGGCAAGAAG
ATAGGCATAGCAACTATAGCTGTAGAGGAGGATGCCTCCCGTAAGCTACGGGAGGAGGGCCGCTACGTACACTACATGCT
TGACCGGCTAGGCATTCCACTCATAGAGATATCGACTGAGCCTGACATTGAGACTCCCGAGGAGGCCTATGAGGCTGCCC
TAGCCATAGGCCAGCTGCTGAGGCTGACCGGCAGGGTAAAGAGGGGTATAGGTACTATACGCCAGGACCTTAACGTCTCA
ATAAGGGGCGGAGTAAAGACCGAGATTAAGGGTGTACAGAGGCTAGAACTCATACCGAAGATAGTCCTCTACGAAGCTAT
TAGACAGAAGAGGCTCCTGGAGATACGCGACGAGCTAAAGAGGAGGGGTGTAAGGCAGGAAGATATCGATGCCCAGGAGA
TAGCCGACGTGACAGATGTGTTTAAGAAGACCAAGTCAAAGATCATAGCTAGAGCAGTCAAGCGTGGAGGCAGGGTTCTA
GCTCTCAAGCTGCCAGGTTTCCACGGCCTACTCGGTGTTGAGGTGCAACCAGGCAGAAGGTTTGGAACAGAGCTAGCAGA
CTATGCACGCTTCTGGGGTGGTGTAGGAGGCCTCTTCCACACCGATGAACTACCAGGCTACGGTATATCAGCTGAAGAAG
TCGAGGAGCTATATAAACTTCTTAATGCCAAGAAGGGCTGGGATGCAATAGTCATAGTAGCGGATGAAGAGCCCAAAGCC
AGGAAGGCACTGGAAGCTGTAAAGGAGAGGGCAAAAATGGCGCTCGAAGGCATACCTAAGGAAACTAGAGCTGCCCAGGA
AGATGGCACAACCAAGTTTATGAGACCACAGCCTGGCTCGGCTAGAATGTATCCAGAGACTGATATACCACCACTAGAAG
TTACCAAGGAGCTGCTCAACAAAGCACGCAAAATAGTGCCGGAAAAGCCCCACGAGAAACTAGAAAAGCTGGTTAGGAAG
TATGGCCTCAGCGAACAGCTAGCAAGACAGATCATAAGGGACATACACCTAGACCTCTTTGAAAAACTCGCCGAGAAGTA
TGGGAGCCGCGTAGCACCATCCTACATAGCATCATTATTCACAAACATACTGAGAAGCCTTGCAAGGGAAGGAGTACCCG
TTAGCAACCTTTCAGACACCCATATAGAAGAGATCGTGAGGGCTATAGCAGAGGGCAAAGTCTCCAAGGATGCTGTGCCA
GACCTCATAAAGTACCTGGCAGAGAATCCATCAAGGAGCGTCGAAGAGGCTGTACGCGAGCTGGGCATAGAGACGGTACC
TGTGGAGAAGGTCGAGAAACTTGTCGAGGAGGCTGTAAAGCAGCTCGAAGCCGAGATTAGGGCGAGAGGGCTGGCGGCAC
TAAACAAGGTTATGGGCCGTGTAATGCCAAAGCTAAGAGGCAAGGTCGACGGTAAGCTGGTAGCAAGTATAGCTAAGCGC
AAAATCGAAGAGCTGCTGGGTGGTAACTAG

Upstream 100 bases:

>100_bases
ACTCCTCGAGTTTTGAGGAGGCAAAGAGACTCATGCGCGAGAACCTAGCTGGAGAGATCAATCCGAGACACACCGTAGAC
CTATACCCGAGGTGGCCGCA

Downstream 100 bases:

>100_bases
TACCGCCACTATATGGATTAGGGAGGCTTCCTCCCCTTTCCTCTTCTGAATGGGCTGTGACGAGTACTCCCGTCGCTGAA
TCGGGAACCGTGATGAGAAG

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E [H]

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MIEPKVDPSKYDPRELGLKAGLEIHQQLNTRAKLFCNCPTRLSSEDEPGDEFVRQLRPTRSELGEVDIAALFEWRKGRMY
HYHAPRATSCLVEADEEPPHEINQEAVVIGLAVALALGATPVDEIHVMRKIVIDGSNTTGFQRTAIIALGGSIELESGKK
IGIATIAVEEDASRKLREEGRYVHYMLDRLGIPLIEISTEPDIETPEEAYEAALAIGQLLRLTGRVKRGIGTIRQDLNVS
IRGGVKTEIKGVQRLELIPKIVLYEAIRQKRLLEIRDELKRRGVRQEDIDAQEIADVTDVFKKTKSKIIARAVKRGGRVL
ALKLPGFHGLLGVEVQPGRRFGTELADYARFWGGVGGLFHTDELPGYGISAEEVEELYKLLNAKKGWDAIVIVADEEPKA
RKALEAVKERAKMALEGIPKETRAAQEDGTTKFMRPQPGSARMYPETDIPPLEVTKELLNKARKIVPEKPHEKLEKLVRK
YGLSEQLARQIIRDIHLDLFEKLAEKYGSRVAPSYIASLFTNILRSLAREGVPVSNLSDTHIEEIVRAIAEGKVSKDAVP
DLIKYLAENPSRSVEEAVRELGIETVPVEKVEKLVEEAVKQLEAEIRARGLAALNKVMGRVMPKLRGKVDGKLVASIAKR
KIEELLGGN

Sequences:

>Translated_649_residues
MIEPKVDPSKYDPRELGLKAGLEIHQQLNTRAKLFCNCPTRLSSEDEPGDEFVRQLRPTRSELGEVDIAALFEWRKGRMY
HYHAPRATSCLVEADEEPPHEINQEAVVIGLAVALALGATPVDEIHVMRKIVIDGSNTTGFQRTAIIALGGSIELESGKK
IGIATIAVEEDASRKLREEGRYVHYMLDRLGIPLIEISTEPDIETPEEAYEAALAIGQLLRLTGRVKRGIGTIRQDLNVS
IRGGVKTEIKGVQRLELIPKIVLYEAIRQKRLLEIRDELKRRGVRQEDIDAQEIADVTDVFKKTKSKIIARAVKRGGRVL
ALKLPGFHGLLGVEVQPGRRFGTELADYARFWGGVGGLFHTDELPGYGISAEEVEELYKLLNAKKGWDAIVIVADEEPKA
RKALEAVKERAKMALEGIPKETRAAQEDGTTKFMRPQPGSARMYPETDIPPLEVTKELLNKARKIVPEKPHEKLEKLVRK
YGLSEQLARQIIRDIHLDLFEKLAEKYGSRVAPSYIASLFTNILRSLAREGVPVSNLSDTHIEEIVRAIAEGKVSKDAVP
DLIKYLAENPSRSVEEAVRELGIETVPVEKVEKLVEEAVKQLEAEIRARGLAALNKVMGRVMPKLRGKVDGKLVASIAKR
KIEELLGGN
>Mature_649_residues
MIEPKVDPSKYDPRELGLKAGLEIHQQLNTRAKLFCNCPTRLSSEDEPGDEFVRQLRPTRSELGEVDIAALFEWRKGRMY
HYHAPRATSCLVEADEEPPHEINQEAVVIGLAVALALGATPVDEIHVMRKIVIDGSNTTGFQRTAIIALGGSIELESGKK
IGIATIAVEEDASRKLREEGRYVHYMLDRLGIPLIEISTEPDIETPEEAYEAALAIGQLLRLTGRVKRGIGTIRQDLNVS
IRGGVKTEIKGVQRLELIPKIVLYEAIRQKRLLEIRDELKRRGVRQEDIDAQEIADVTDVFKKTKSKIIARAVKRGGRVL
ALKLPGFHGLLGVEVQPGRRFGTELADYARFWGGVGGLFHTDELPGYGISAEEVEELYKLLNAKKGWDAIVIVADEEPKA
RKALEAVKERAKMALEGIPKETRAAQEDGTTKFMRPQPGSARMYPETDIPPLEVTKELLNKARKIVPEKPHEKLEKLVRK
YGLSEQLARQIIRDIHLDLFEKLAEKYGSRVAPSYIASLFTNILRSLAREGVPVSNLSDTHIEEIVRAIAEGKVSKDAVP
DLIKYLAENPSRSVEEAVRELGIETVPVEKVEKLVEEAVKQLEAEIRARGLAALNKVMGRVMPKLRGKVDGKLVASIAKR
KIEELLGGN

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 72272; Mature: 72272

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEPKVDPSKYDPRELGLKAGLEIHQQLNTRAKLFCNCPTRLSSEDEPGDEFVRQLRPTR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHCCHH
SELGEVDIAALFEWRKGRMYHYHAPRATSCLVEADEEPPHEINQEAVVIGLAVALALGAT
HHHCCHHHHHHHHHHCCCEEEECCCCHHHHHEECCCCCCCHHCCHHHHHHHHHHHHHCCC
PVDEIHVMRKIVIDGSNTTGFQRTAIIALGGSIELESGKKIGIATIAVEEDASRKLREEG
CHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEEEECCCCEEEEEEEEECCHHHHHHHHCC
RYVHYMLDRLGIPLIEISTEPDIETPEEAYEAALAIGQLLRLTGRVKRGIGTIRQDLNVS
CHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
IRGGVKTEIKGVQRLELIPKIVLYEAIRQKRLLEIRDELKRRGVRQEDIDAQEIADVTDV
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FKKTKSKIIARAVKRGGRVLALKLPGFHGLLGVEVQPGRRFGTELADYARFWGGVGGLFH
HHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
TDELPGYGISAEEVEELYKLLNAKKGWDAIVIVADEEPKARKALEAVKERAKMALEGIPK
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCH
ETRAAQEDGTTKFMRPQPGSARMYPETDIPPLEVTKELLNKARKIVPEKPHEKLEKLVRK
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YGLSEQLARQIIRDIHLDLFEKLAEKYGSRVAPSYIASLFTNILRSLAREGVPVSNLSDT
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
HIEEIVRAIAEGKVSKDAVPDLIKYLAENPSRSVEEAVRELGIETVPVEKVEKLVEEAVK
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QLEAEIRARGLAALNKVMGRVMPKLRGKVDGKLVASIAKRKIEELLGGN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEPKVDPSKYDPRELGLKAGLEIHQQLNTRAKLFCNCPTRLSSEDEPGDEFVRQLRPTR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHCCHH
SELGEVDIAALFEWRKGRMYHYHAPRATSCLVEADEEPPHEINQEAVVIGLAVALALGAT
HHHCCHHHHHHHHHHCCCEEEECCCCHHHHHEECCCCCCCHHCCHHHHHHHHHHHHHCCC
PVDEIHVMRKIVIDGSNTTGFQRTAIIALGGSIELESGKKIGIATIAVEEDASRKLREEG
CHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEEEECCCCEEEEEEEEECCHHHHHHHHCC
RYVHYMLDRLGIPLIEISTEPDIETPEEAYEAALAIGQLLRLTGRVKRGIGTIRQDLNVS
CHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
IRGGVKTEIKGVQRLELIPKIVLYEAIRQKRLLEIRDELKRRGVRQEDIDAQEIADVTDV
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FKKTKSKIIARAVKRGGRVLALKLPGFHGLLGVEVQPGRRFGTELADYARFWGGVGGLFH
HHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
TDELPGYGISAEEVEELYKLLNAKKGWDAIVIVADEEPKARKALEAVKERAKMALEGIPK
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCH
ETRAAQEDGTTKFMRPQPGSARMYPETDIPPLEVTKELLNKARKIVPEKPHEKLEKLVRK
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YGLSEQLARQIIRDIHLDLFEKLAEKYGSRVAPSYIASLFTNILRSLAREGVPVSNLSDT
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
HIEEIVRAIAEGKVSKDAVPDLIKYLAENPSRSVEEAVRELGIETVPVEKVEKLVEEAVK
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QLEAEIRARGLAALNKVMGRVMPKLRGKVDGKLVASIAKRKIEELLGGN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]