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The map label for this gene is proB [H]

Identifier: 123966028

GI number: 123966028

Start: 715311

End: 716393

Strand: Direct

Name: proB [H]

Synonym: P9515_07931

Alternate gene names: 123966028

Gene position: 715311-716393 (Clockwise)

Preceding gene: 123966027

Following gene: 123966029

Centisome position: 41.97

GC content: 29.27

Gene sequence:

>1083_bases
ATGAAAACGTGGGTCGTTAAAGTTGGTACTAGTATTCTCAGAGGGACAAATGAGAAATCTACAGAACAAGTAATAGAAAG
TTTATGTAAATCTTTAACAAGTTTTATTTTGAGGGGTAATAAAGTGGTTCTAGTAACCAGTGGAGCAGTTGGATTAGGAT
GTAATAAATTAAATTTGAATAGAAGGCCAAAGGAACTTAGTTCTCTACAAGCTGTAGCTGCTGTAGGACAAGTAAATTTA
ATGACTTTGTATGATAAAACAATGAAAAAGCTAGGTCATAATATAGCACAAATACTCATAACAAAAACTGATTTCAATTC
GCGTGAGTCTTTCAATAATGCATCCAAAACATTTAAAAAATTAATTGATCTTAATGTCATCCCGATAGTTAATGAAAATG
ATTCTATTGCTAATGAAGAGCTAAAATATGGAGATAATGATACTCTCTCTGCATTAGTTGCTTTAGCAATTAATGCAAAT
AAGCTTATTTTATTAACTGATATAGAAAATTTATATTCTAAAGATCCCAGAAATAATAAGGATGCTCATCCAATAAGAGA
AGTTAATACTAATGAATTAAAAATTATTAGAGATAAAAATAATAAAATTTATAATAATGAATGGGGGACAGGAGGCATTA
CTACAAAACTTATTGCTGCTGAAATTGCTACAAAAGGAGGAATAGAAGTTCAATTGGCCGATGGCAGAAATGAAAATAAT
TTAATAGATATTTTTAATGATAAAAAAATAGGTACAATATTTCATCCTTTAGATAAACCTGTTGGTAATAAAAAAAATTG
GTTATCTCATGCGATTAAAACTGTTGGAGAAATTACTTTAGATGAAGGTGCATGTTTAGCTATTGTAAAAAAAGGAGCGT
CTCTTTTAGTCGTTGGAGTTAAAGAAGTAGAGGGAGATTTTACGGTTAATCAAGCTGTTAGAATTGTAAATACAGATAAG
AAAGAAGTCGCAAAAGGTATAACATCAATGAGTAGTGATTCTCTAAAAAGAATTTTAAATAAAAAAGAAACCATTAATTC
ATCAATGATAGTGGTACATAGAGATGTTCTTGCTCTTTCTTAA

Upstream 100 bases:

>100_bases
ATATCCAGACGATCTTAGTAAAAAGATTAAGCAGAGAGGGTTTGCCGATAAAAATAAATTTAACATTAATACTAGAAAAA
TTGATTTCTACTTTTTTAAA

Downstream 100 bases:

>100_bases
AAAATAAATTAAAAAATGTTATTAAGTAAATTGGTTGATCTTATAAAAAGTGGAAATTCAAACTTTATTAAAGCAAATAT
ATTTGAAAATATAGATATAC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTWVVKVGTSILRGTNEKSTEQVIESLCKSLTSFILRGNKVVLVTSGAVGLGCNKLNLNRRPKELSSLQAVAAVGQVNL
MTLYDKTMKKLGHNIAQILITKTDFNSRESFNNASKTFKKLIDLNVIPIVNENDSIANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRNNKDAHPIREVNTNELKIIRDKNNKIYNNEWGTGGITTKLIAAEIATKGGIEVQLADGRNENN
LIDIFNDKKIGTIFHPLDKPVGNKKNWLSHAIKTVGEITLDEGACLAIVKKGASLLVVGVKEVEGDFTVNQAVRIVNTDK
KEVAKGITSMSSDSLKRILNKKETINSSMIVVHRDVLALS

Sequences:

>Translated_360_residues
MKTWVVKVGTSILRGTNEKSTEQVIESLCKSLTSFILRGNKVVLVTSGAVGLGCNKLNLNRRPKELSSLQAVAAVGQVNL
MTLYDKTMKKLGHNIAQILITKTDFNSRESFNNASKTFKKLIDLNVIPIVNENDSIANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRNNKDAHPIREVNTNELKIIRDKNNKIYNNEWGTGGITTKLIAAEIATKGGIEVQLADGRNENN
LIDIFNDKKIGTIFHPLDKPVGNKKNWLSHAIKTVGEITLDEGACLAIVKKGASLLVVGVKEVEGDFTVNQAVRIVNTDK
KEVAKGITSMSSDSLKRILNKKETINSSMIVVHRDVLALS
>Mature_360_residues
MKTWVVKVGTSILRGTNEKSTEQVIESLCKSLTSFILRGNKVVLVTSGAVGLGCNKLNLNRRPKELSSLQAVAAVGQVNL
MTLYDKTMKKLGHNIAQILITKTDFNSRESFNNASKTFKKLIDLNVIPIVNENDSIANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRNNKDAHPIREVNTNELKIIRDKNNKIYNNEWGTGGITTKLIAAEIATKGGIEVQLADGRNENN
LIDIFNDKKIGTIFHPLDKPVGNKKNWLSHAIKTVGEITLDEGACLAIVKKGASLLVVGVKEVEGDFTVNQAVRIVNTDK
KEVAKGITSMSSDSLKRILNKKETINSSMIVVHRDVLALS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=384, Percent_Identity=26.8229166666667, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI21361368, Length=386, Percent_Identity=26.6839378238342, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=37.9120879120879, Blast_Score=255, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17569967, Length=321, Percent_Identity=28.6604361370716, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17569969, Length=321, Percent_Identity=28.6604361370716, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=30.7692307692308, Blast_Score=179, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321822, Length=228, Percent_Identity=40.7894736842105, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=32.7526132404181, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39499; Mature: 39499

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTWVVKVGTSILRGTNEKSTEQVIESLCKSLTSFILRGNKVVLVTSGAVGLGCNKLNLN
CCEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
RRPKELSSLQAVAAVGQVNLMTLYDKTMKKLGHNIAQILITKTDFNSRESFNNASKTFKK
CCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCHHEEEEEEECCCCCHHHCCHHHHHHHH
LIDLNVIPIVNENDSIANEELKYGDNDTLSALVALAINANKLILLTDIENLYSKDPRNNK
HHCCCEEEEECCCCCCCCCHHCCCCCHHHHHHHHHEECCCEEEEEECCHHHHCCCCCCCC
DAHPIREVNTNELKIIRDKNNKIYNNEWGTGGITTKLIAAEIATKGGIEVQLADGRNENN
CCCCCCCCCCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCC
LIDIFNDKKIGTIFHPLDKPVGNKKNWLSHAIKTVGEITLDEGACLAIVKKGASLLVVGV
EEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCEEECCCCEEEEECCCCEEEEEEE
KEVEGDFTVNQAVRIVNTDKKEVAKGITSMSSDSLKRILNKKETINSSMIVVHRDVLALS
ECCCCCEEHHHEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECEECCC
>Mature Secondary Structure
MKTWVVKVGTSILRGTNEKSTEQVIESLCKSLTSFILRGNKVVLVTSGAVGLGCNKLNLN
CCEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
RRPKELSSLQAVAAVGQVNLMTLYDKTMKKLGHNIAQILITKTDFNSRESFNNASKTFKK
CCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCHHEEEEEEECCCCCHHHCCHHHHHHHH
LIDLNVIPIVNENDSIANEELKYGDNDTLSALVALAINANKLILLTDIENLYSKDPRNNK
HHCCCEEEEECCCCCCCCCHHCCCCCHHHHHHHHHEECCCEEEEEECCHHHHCCCCCCCC
DAHPIREVNTNELKIIRDKNNKIYNNEWGTGGITTKLIAAEIATKGGIEVQLADGRNENN
CCCCCCCCCCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCC
LIDIFNDKKIGTIFHPLDKPVGNKKNWLSHAIKTVGEITLDEGACLAIVKKGASLLVVGV
EEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCEEECCCCEEEEECCCCEEEEEEE
KEVEGDFTVNQAVRIVNTDKKEVAKGITSMSSDSLKRILNKKETINSSMIVVHRDVLALS
ECCCCCEEHHHEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]