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The map label for this gene is capI [H]

Identifier: 123968930

GI number: 123968930

Start: 1165644

End: 1166672

Strand: Reverse

Name: capI [H]

Synonym: A9601_13971

Alternate gene names: 123968930

Gene position: 1166672-1165644 (Counterclockwise)

Preceding gene: 123968935

Following gene: 123968927

Centisome position: 69.87

GC content: 27.89

Gene sequence:

>1029_bases
TTGAATAATAATCGCATCTTGATAACTGGGGCTGCAGGATTTATAGGTTCTGCTCTAATTTTAAGATTATTAGAAAACGA
AAAAACTATTATTGGAGTTGATAATTTAAATAATTATTATGATGTAAGACTTAAAAAATCTAGATTAAAATTAATTACTG
AAAAATCAAAAAAGCTTAAAGCAAATTGGATTTTTCATGAATTCCATATTGAAGATAAGAAATCATTAGATTTTATTACA
GAAAAATATAGCCCTAGCATCGTTATTCATCTTGCAGCTCAAGCAGGTGTCAGATATTCATTAGATAATCCAAAATCTTA
TGCAGATTCAAACTTAATCGGATTCTTTAATATTTTGGAATTCTGCAAAGAAAATAAAGTTAAAAATTTTGTTTTTGCAT
CTAGTAGCTCAGTTTATGGACTTAATAAAAAAATTCCATTTGTAGAGGATGATAATGTTGATCATCCAATAAGTTTTTAT
GCAGCAACAAAAAAGTCCAATGAACTTATGGCTCATAGTTACAGTCACCTTTATGATATACCAACTACAGGATTAAGATT
TTTTACGGTATATGGTCCTTTTGGTAGGCCAGATATGGCACCAATGATTTTTGCTAATGCGATCTTAAATTCTAAACCAA
TAAATATTTTTAATTATGGGAATTTACATAGAGACTTCACCTATATTGATGATATCGTAAATGGCTTGTTTGGTTGCTGT
TATAAGCCTGCAATAAAAAGCGAAAATTTTAGTTCAAACTATCAAAATAAATCTTATTCAAACGCACCTTTTCAAATATT
CAATATTGGAAATAGTAATCCTATAAAAATAGATTATTTTATAAGTATGCTTGAGCTAAATTTCAACAAAAAGGCAATTA
TAAACTTAATGCCTCTTCAGCCAGGAGATGTAAAATTTACCTACGCTGATATATCAAAAATTCAAAAATGGATAGGTTAT
AAACCAAAAGTATCTTTTGAAAAGGGGATTAGAGAATTCTCAAAATGGTATTTAGATTTCTATGAATAG

Upstream 100 bases:

>100_bases
AGAATAACAGGTATGATCACTTTGGAATTCATAAATATATATATAATTATTTATTATATTAATAAAGTAGTTATAATATT
TAAACAGAAAAGAATCCTTT

Downstream 100 bases:

>100_bases
AAAGTTTAAGTTCTTAACTTAGGCTAATAATTTTTTCTATTGTTAAATATTAAAAATTTATGAACTTGGTTAGGAAACTA
CTCAAGAATAATAGTATTGA

Product: putative nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNNNRILITGAAGFIGSALILRLLENEKTIIGVDNLNNYYDVRLKKSRLKLITEKSKKLKANWIFHEFHIEDKKSLDFIT
EKYSPSIVIHLAAQAGVRYSLDNPKSYADSNLIGFFNILEFCKENKVKNFVFASSSSVYGLNKKIPFVEDDNVDHPISFY
AATKKSNELMAHSYSHLYDIPTTGLRFFTVYGPFGRPDMAPMIFANAILNSKPINIFNYGNLHRDFTYIDDIVNGLFGCC
YKPAIKSENFSSNYQNKSYSNAPFQIFNIGNSNPIKIDYFISMLELNFNKKAIINLMPLQPGDVKFTYADISKIQKWIGY
KPKVSFEKGIREFSKWYLDFYE

Sequences:

>Translated_342_residues
MNNNRILITGAAGFIGSALILRLLENEKTIIGVDNLNNYYDVRLKKSRLKLITEKSKKLKANWIFHEFHIEDKKSLDFIT
EKYSPSIVIHLAAQAGVRYSLDNPKSYADSNLIGFFNILEFCKENKVKNFVFASSSSVYGLNKKIPFVEDDNVDHPISFY
AATKKSNELMAHSYSHLYDIPTTGLRFFTVYGPFGRPDMAPMIFANAILNSKPINIFNYGNLHRDFTYIDDIVNGLFGCC
YKPAIKSENFSSNYQNKSYSNAPFQIFNIGNSNPIKIDYFISMLELNFNKKAIINLMPLQPGDVKFTYADISKIQKWIGY
KPKVSFEKGIREFSKWYLDFYE
>Mature_342_residues
MNNNRILITGAAGFIGSALILRLLENEKTIIGVDNLNNYYDVRLKKSRLKLITEKSKKLKANWIFHEFHIEDKKSLDFIT
EKYSPSIVIHLAAQAGVRYSLDNPKSYADSNLIGFFNILEFCKENKVKNFVFASSSSVYGLNKKIPFVEDDNVDHPISFY
AATKKSNELMAHSYSHLYDIPTTGLRFFTVYGPFGRPDMAPMIFANAILNSKPINIFNYGNLHRDFTYIDDIVNGLFGCC
YKPAIKSENFSSNYQNKSYSNAPFQIFNIGNSNPIKIDYFISMLELNFNKKAIINLMPLQPGDVKFTYADISKIQKWIGY
KPKVSFEKGIREFSKWYLDFYE

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=355, Percent_Identity=25.9154929577465, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI56118217, Length=355, Percent_Identity=25.9154929577465, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI189083684, Length=355, Percent_Identity=25.9154929577465, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI7657641, Length=360, Percent_Identity=25.2777777777778, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=26.1127596439169, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=25.414364640884, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI48994969, Length=360, Percent_Identity=28.0555555555556, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786974, Length=350, Percent_Identity=25.1428571428571, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=357, Percent_Identity=24.6498599439776, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=358, Percent_Identity=25.4189944134078, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17539532, Length=337, Percent_Identity=27.299703264095, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=360, Percent_Identity=25.2777777777778, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=142, Percent_Identity=34.5070422535211, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19923002, Length=353, Percent_Identity=26.628895184136, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356223, Length=341, Percent_Identity=25.2199413489736, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 39338; Mature: 39338

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNRILITGAAGFIGSALILRLLENEKTIIGVDNLNNYYDVRLKKSRLKLITEKSKKLK
CCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHCHHEE
ANWIFHEFHIEDKKSLDFITEKYSPSIVIHLAAQAGVRYSLDNPKSYADSNLIGFFNILE
EEEEEEEEECCCCCCHHHHHHCCCCEEEEEEECCCCCEEECCCCHHHCCCCHHHHHHHHH
FCKENKVKNFVFASSSSVYGLNKKIPFVEDDNVDHPISFYAATKKSNELMAHSYSHLYDI
HHHCCCCCEEEEECCCCEEECCCCCCEECCCCCCCCEEEEEECCCCCHHHHHHHHHEECC
PTTGLRFFTVYGPFGRPDMAPMIFANAILNSKPINIFNYGNLHRDFTYIDDIVNGLFGCC
CCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
YKPAIKSENFSSNYQNKSYSNAPFQIFNIGNSNPIKIDYFISMLELNFNKKAIINLMPLQ
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEEEEECCC
PGDVKFTYADISKIQKWIGYKPKVSFEKGIREFSKWYLDFYE
CCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNNRILITGAAGFIGSALILRLLENEKTIIGVDNLNNYYDVRLKKSRLKLITEKSKKLK
CCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHCHHEE
ANWIFHEFHIEDKKSLDFITEKYSPSIVIHLAAQAGVRYSLDNPKSYADSNLIGFFNILE
EEEEEEEEECCCCCCHHHHHHCCCCEEEEEEECCCCCEEECCCCHHHCCCCHHHHHHHHH
FCKENKVKNFVFASSSSVYGLNKKIPFVEDDNVDHPISFYAATKKSNELMAHSYSHLYDI
HHHCCCCCEEEEECCCCEEECCCCCCEECCCCCCCCEEEEEECCCCCHHHHHHHHHEECC
PTTGLRFFTVYGPFGRPDMAPMIFANAILNSKPINIFNYGNLHRDFTYIDDIVNGLFGCC
CCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
YKPAIKSENFSSNYQNKSYSNAPFQIFNIGNSNPIKIDYFISMLELNFNKKAIINLMPLQ
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEEEEECCC
PGDVKFTYADISKIQKWIGYKPKVSFEKGIREFSKWYLDFYE
CCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]