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The map label for this gene is gid [H]

Identifier: 123968755

GI number: 123968755

Start: 1039754

End: 1041166

Strand: Reverse

Name: gid [H]

Synonym: A9601_12221

Alternate gene names: 123968755

Gene position: 1041166-1039754 (Counterclockwise)

Preceding gene: 123968756

Following gene: 123968754

Centisome position: 62.35

GC content: 33.4

Gene sequence:

>1413_bases
TTGTCATATAAACAAGTAATAATTATTGGAGCTGGTCTCGCAGGATCAGAAGCAGCTTGGCAAGTTGCAAGTTCTGGTGT
TCCAGTTAAATTAGTTGAGATGAGGCCTATTAAATCAACTCCAGCTCATCATACAAGTGAATTTGGAGAATTGGTTTGTA
GTAATAGCTTTGGAGCTCTAAGCCCTGATAGAGCTGCCGGTTTATTACAAAAAGAACTTAGAATTTTTAATTCATTGATA
GTTCAAACAGCAGATAAATTTGCTGTTCCAGCTGGAGGCGCTTTGGCGGTTGATAGATCTAAATTCAGTATTGCTTTGAC
TGAAGCTTTATCTAATCATCCTTTAATTGAAATTAAGAGATTTGAACAATTAGATTTGCCAAGCAAAGAAAATATAACTA
TCCTTGCAACTGGTCCATTAACTGCAGATGAATTATCCTATAAAATTCAAGCTTTTACAGGTATAGATGCGTGTCATTTT
TTTGATGCCGCTAGTCCTATTATTTATGGAGATACTATTGATCAAGAGATTGTATTTAAAGCTAGTAGATATGACAAAGG
AGATCCTGCATATTTTAATTGCCCTATGGGAAAAAATGATTATATCAATTTCAGAAATGAACTAATAAAAGGTGAACAAG
TTAATTTAAAAGACTTTGAGAAAGAATCAGCTAATTTCTTTGAAGCTTGTTTACCAATTGAAGAAATTGCTAGAAGAGGA
GTTGATACAATGAGGTACGGACCACTAAAATCAATTGGTTTGTGGAATCCAAAATGGGGAGATTTATTTGATAGGGAAAA
TAGATTAAAAAAGCGACCTCATGCAGTTGTCCAATTAAGGAAAGAAGATTTAGAAGGAAAATTGCTAAATATGGTAGGTT
TTCAAACTAACCTCAAATGGTCTGAGCAAAAAAGAATATTTAGGTTGATTCCTGGTTTAGAAAAGGCTGAGTTTGTACGT
TTTGGAGTAATGCATAGAAATACTTTTTTAGAATCTCCAAAATTACTTTTACCGACATTACAATTTTTGAAAAGAGAAAA
CCTTTTTGCTGCAGGCCAAATAACGGGTACCGAAGGTTATGCAGCAGCAGCAGCAGGGGGCTTGCTTGCAGGAATAAATG
CATCCTTATTAGCTAAGGGTAAAAAAACAGTAAGTTTCCCTGATCAATCAATGATTGGTTCTCTAATGAATTTTATCAGT
AACAAAAATCAAATATTATCTAATCAGCAAAAGAATAAATTCCAACCAATGCCCGCTTCATTTGGTTTAGTTCCAGAGCT
AATTAAAAGAATAAAAGATAAAAGATTAAGGTACAAAGCTTATCAAGAAAGATCTACAGAAGCCTTGAATGACTTTAAAA
ATCAACTAGATTCTTGTTTTGATAAAGACCACTTACTTAGCAAAATTTACTAA

Upstream 100 bases:

>100_bases
TGCACCCATTATTGCGGCTTTAGCTTGGGTCATATTCAACATACAAAAACCAGCAAGAGAGCAATTCAATAGGGACTTTT
TGGGCAAGGATTAATCCAAT

Downstream 100 bases:

>100_bases
GATTAATTATCAAAGATCCAAAAAATGGAATTAAATAAGGAAAACTTCGATGCAATTATTATTGGCTCAGGAATTGGAGG
ATTAGTAACTGCTTCACAAT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSYKQVIIIGAGLAGSEAAWQVASSGVPVKLVEMRPIKSTPAHHTSEFGELVCSNSFGALSPDRAAGLLQKELRIFNSLI
VQTADKFAVPAGGALAVDRSKFSIALTEALSNHPLIEIKRFEQLDLPSKENITILATGPLTADELSYKIQAFTGIDACHF
FDAASPIIYGDTIDQEIVFKASRYDKGDPAYFNCPMGKNDYINFRNELIKGEQVNLKDFEKESANFFEACLPIEEIARRG
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAVVQLRKEDLEGKLLNMVGFQTNLKWSEQKRIFRLIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFLKRENLFAAGQITGTEGYAAAAAGGLLAGINASLLAKGKKTVSFPDQSMIGSLMNFIS
NKNQILSNQQKNKFQPMPASFGLVPELIKRIKDKRLRYKAYQERSTEALNDFKNQLDSCFDKDHLLSKIY

Sequences:

>Translated_470_residues
MSYKQVIIIGAGLAGSEAAWQVASSGVPVKLVEMRPIKSTPAHHTSEFGELVCSNSFGALSPDRAAGLLQKELRIFNSLI
VQTADKFAVPAGGALAVDRSKFSIALTEALSNHPLIEIKRFEQLDLPSKENITILATGPLTADELSYKIQAFTGIDACHF
FDAASPIIYGDTIDQEIVFKASRYDKGDPAYFNCPMGKNDYINFRNELIKGEQVNLKDFEKESANFFEACLPIEEIARRG
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAVVQLRKEDLEGKLLNMVGFQTNLKWSEQKRIFRLIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFLKRENLFAAGQITGTEGYAAAAAGGLLAGINASLLAKGKKTVSFPDQSMIGSLMNFIS
NKNQILSNQQKNKFQPMPASFGLVPELIKRIKDKRLRYKAYQERSTEALNDFKNQLDSCFDKDHLLSKIY
>Mature_469_residues
SYKQVIIIGAGLAGSEAAWQVASSGVPVKLVEMRPIKSTPAHHTSEFGELVCSNSFGALSPDRAAGLLQKELRIFNSLIV
QTADKFAVPAGGALAVDRSKFSIALTEALSNHPLIEIKRFEQLDLPSKENITILATGPLTADELSYKIQAFTGIDACHFF
DAASPIIYGDTIDQEIVFKASRYDKGDPAYFNCPMGKNDYINFRNELIKGEQVNLKDFEKESANFFEACLPIEEIARRGV
DTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAVVQLRKEDLEGKLLNMVGFQTNLKWSEQKRIFRLIPGLEKAEFVRF
GVMHRNTFLESPKLLLPTLQFLKRENLFAAGQITGTEGYAAAAAGGLLAGINASLLAKGKKTVSFPDQSMIGSLMNFISN
KNQILSNQQKNKFQPMPASFGLVPELIKRIKDKRLRYKAYQERSTEALNDFKNQLDSCFDKDHLLSKIY

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=421, Percent_Identity=25.1781472684086, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17534255, Length=437, Percent_Identity=25.629290617849, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321202, Length=519, Percent_Identity=25.4335260115607, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 52537; Mature: 52406

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKQVIIIGAGLAGSEAAWQVASSGVPVKLVEMRPIKSTPAHHTSEFGELVCSNSFGAL
CCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCC
SPDRAAGLLQKELRIFNSLIVQTADKFAVPAGGALAVDRSKFSIALTEALSNHPLIEIKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHCCCCEEEEEC
FEQLDLPSKENITILATGPLTADELSYKIQAFTGIDACHFFDAASPIIYGDTIDQEIVFK
HHHCCCCCCCCEEEEEECCCCHHHHCEEEEEECCCCHHHHHCCCCCEEECCCCCHHHHEE
ASRYDKGDPAYFNCPMGKNDYINFRNELIKGEQVNLKDFEKESANFFEACLPIEEIARRG
HHCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCC
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAVVQLRKEDLEGKLLNMVGFQTNLKW
CCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHCCCCCCCH
SEQKRIFRLIPGLEKAEFVRFGVMHRNTFLESPKLLLPTLQFLKRENLFAAGQITGTEGY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECEEECCCCC
AAAAAGGLLAGINASLLAKGKKTVSFPDQSMIGSLMNFISNKNQILSNQQKNKFQPMPAS
HHHHHCCHHHCCCHHHHHCCCCEECCCCHHHHHHHHHHHCCCHHHHHCCHHCCCCCCCCC
FGLVPELIKRIKDKRLRYKAYQERSTEALNDFKNQLDSCFDKDHLLSKIY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure 
SYKQVIIIGAGLAGSEAAWQVASSGVPVKLVEMRPIKSTPAHHTSEFGELVCSNSFGAL
CCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCC
SPDRAAGLLQKELRIFNSLIVQTADKFAVPAGGALAVDRSKFSIALTEALSNHPLIEIKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHCCCCEEEEEC
FEQLDLPSKENITILATGPLTADELSYKIQAFTGIDACHFFDAASPIIYGDTIDQEIVFK
HHHCCCCCCCCEEEEEECCCCHHHHCEEEEEECCCCHHHHHCCCCCEEECCCCCHHHHEE
ASRYDKGDPAYFNCPMGKNDYINFRNELIKGEQVNLKDFEKESANFFEACLPIEEIARRG
HHCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCC
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAVVQLRKEDLEGKLLNMVGFQTNLKW
CCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHCCCCCCCH
SEQKRIFRLIPGLEKAEFVRFGVMHRNTFLESPKLLLPTLQFLKRENLFAAGQITGTEGY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECEEECCCCC
AAAAAGGLLAGINASLLAKGKKTVSFPDQSMIGSLMNFISNKNQILSNQQKNKFQPMPAS
HHHHHCCHHHCCCHHHHHCCCCEECCCCHHHHHHHHHHHCCCHHHHHCCHHCCCCCCCCC
FGLVPELIKRIKDKRLRYKAYQERSTEALNDFKNQLDSCFDKDHLLSKIY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA