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The map label for this gene is purA [H]

Identifier: 123968098

GI number: 123968098

Start: 489451

End: 490761

Strand: Reverse

Name: purA [H]

Synonym: A9601_05631

Alternate gene names: 123968098

Gene position: 490761-489451 (Counterclockwise)

Preceding gene: 123968099

Following gene: 123968097

Centisome position: 29.39

GC content: 38.22

Gene sequence:

>1311_bases
TTGGCCAATGTTGTTGTAATCGGAGCCCAATGGGGTGACGAAGGAAAAGGTAAAATAACGGATTTACTTAGTCGTTCGGC
AGATGTTGTCGTTCGCTATCAAGGGGGAGTAAATGCAGGTCATACAATAGTGGTAGATGATAAAGTCTTAAAATTACATT
TAATTCCCTCAGGGATACTTTATAAAAATACTTCTTGTCTAATTGGTTCGGGAACTGTTATAGATCCAAAAATCTTGCTT
AAAGAAATTGACATGCTAATTGATAATGGAATTGATATCTCAGGATTAAAAATTTCATCAACATCACATGTAACAATGCC
CTACCACCGAATATTAGATGAGGCGATGGAGGCTGATAGAGGTTCAAACAAAATAGGGACAACAGGTCGTGGAATTGGCC
CAACTTATGCGGATAAGTCACAAAGAAATGGCATTAGGATAAGAGACTTGCTCAATAAAGAAAGGCTAAGTGATGTGATC
GAAATTCCATTAAGAGAAAAAAACGGTCTTCTAGAAAAAATCTATGGCATAAAACCACTTAAATTAGAGGATATTGTTGA
AGAATACCTTGACTATGGGGAAAGATTATCAAAGCATGTTGTTGACTGTACGAGGACTATCCATGCAGCCTCAAAAAACA
AGAAGAATATTCTTTTCGAAGGTGCTCAAGGGACTCTACTTGACTTAGATCATGGTACTTATCCTTTTGTTACCTCATCA
AACCCTATATCAGGAGGGGCATGTATTGGGGCTGGAGTGGGTCCAACTTTAATCGATAGAGTCATAGGTGTCGCAAAAGC
TTACACCACAAGAGTAGGTGAAGGGCCATTCCCAACAGAACTGCAAGGGAGTATTAATGATCAACTCTGTGATAGAGGCA
GTGAATTTGGAACCACTACTGGGAGAAGGAGAAGATGTGGGTGGTTTGATGGAGTTATTGGTAAATATGCTGTATCTGTA
AATGGTCTTGATTGTTTAGCCGTTACAAAACTTGATGTGTTAGATGAGTTAGATGAGATTCAAGTTTGCATTGCATATGA
CCTCAATGGAGAGGAAATAGACTACTTTCCTACAAATTCAGATGACTTAAAGAAATGTAAGCCAATCTTCAAAAAATTAA
AAGGTTGGCAATGTTCAACTGCAGATTGCAGAAAACTATCTGATCTCCCAGAGAATGCTATGAATTATCTAAGATTTTTA
GCTGAATTAATGGAGGTTCCAATTGCCATTGTCTCGTTGGGGGCAAATAGAGATCAAACCATTGTAATTGAAGACCCAAT
ACATGGTCCTAAAAGAGCACTGCTAAGGTAA

Upstream 100 bases:

>100_bases
GGTTCTAAGAGAAAGTTGATACAATTCATTTACTTTTTAAAAGTAAACCAAGATTTTTGAATATATTAAATATTCGCACA
AAAATAATGCTCTTCTAAAA

Downstream 100 bases:

>100_bases
TTTAGTTCCAAATTAATGAAGGAATCCTTTAGACATTTTGAACATAAAAAAGTTGATCTCATTGGTCTGGGCAACGCAAT
AGTAGATATTATTGTAAATA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYKNTSCLIGSGTVIDPKILL
KEIDMLIDNGIDISGLKISSTSHVTMPYHRILDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNKERLSDVI
EIPLREKNGLLEKIYGIKPLKLEDIVEEYLDYGERLSKHVVDCTRTIHAASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVSV
NGLDCLAVTKLDVLDELDEIQVCIAYDLNGEEIDYFPTNSDDLKKCKPIFKKLKGWQCSTADCRKLSDLPENAMNYLRFL
AELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR

Sequences:

>Translated_436_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYKNTSCLIGSGTVIDPKILL
KEIDMLIDNGIDISGLKISSTSHVTMPYHRILDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNKERLSDVI
EIPLREKNGLLEKIYGIKPLKLEDIVEEYLDYGERLSKHVVDCTRTIHAASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVSV
NGLDCLAVTKLDVLDELDEIQVCIAYDLNGEEIDYFPTNSDDLKKCKPIFKKLKGWQCSTADCRKLSDLPENAMNYLRFL
AELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR
>Mature_435_residues
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYKNTSCLIGSGTVIDPKILLK
EIDMLIDNGIDISGLKISSTSHVTMPYHRILDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNKERLSDVIE
IPLREKNGLLEKIYGIKPLKLEDIVEEYLDYGERLSKHVVDCTRTIHAASKNKKNILFEGAQGTLLDLDHGTYPFVTSSN
PISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVSVN
GLDCLAVTKLDVLDELDEIQVCIAYDLNGEEIDYFPTNSDDLKKCKPIFKKLKGWQCSTADCRKLSDLPENAMNYLRFLA
ELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=42.2535211267606, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=44.6009389671362, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=42.6065162907268, Blast_Score=291, Evalue=1e-78,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=46.6042154566745, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=42.5925925925926, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=42.5925925925926, Blast_Score=312, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=43.6194895591647, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=42.5882352941177, Blast_Score=338, Evalue=4e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47725; Mature: 47594

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGIL
CCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCEE
YKNTSCLIGSGTVIDPKILLKEIDMLIDNGIDISGLKISSTSHVTMPYHRILDEAMEADR
EECCEEEEECCCEECHHHHHHHHHHHHHCCCCCCCEEECCCCCEECHHHHHHHHHHHHCC
GSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNKERLSDVIEIPLREKNGLLEKIYGIKPL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHCCCCC
KLEDIVEEYLDYGERLSKHVVDCTRTIHAASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCEEECC
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
GRRRRCGWFDGVIGKYAVSVNGLDCLAVTKLDVLDELDEIQVCIAYDLNGEEIDYFPTNS
CCCCCCCHHHHHHHHEEEEECCCEEEEEHHHHHHHHHCCEEEEEEEECCCCEEEECCCCC
DDLKKCKPIFKKLKGWQCSTADCRKLSDLPENAMNYLRFLAELMEVPIAIVSLGANRDQT
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCE
IVIEDPIHGPKRALLR
EEEECCCCCCHHHHCC
>Mature Secondary Structure 
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGIL
CCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCEE
YKNTSCLIGSGTVIDPKILLKEIDMLIDNGIDISGLKISSTSHVTMPYHRILDEAMEADR
EECCEEEEECCCEECHHHHHHHHHHHHHCCCCCCCEEECCCCCEECHHHHHHHHHHHHCC
GSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNKERLSDVIEIPLREKNGLLEKIYGIKPL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHCCCCC
KLEDIVEEYLDYGERLSKHVVDCTRTIHAASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCEEECC
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
GRRRRCGWFDGVIGKYAVSVNGLDCLAVTKLDVLDELDEIQVCIAYDLNGEEIDYFPTNS
CCCCCCCHHHHHHHHEEEEECCCEEEEEHHHHHHHHHCCEEEEEEEECCCCEEEECCCCC
DDLKKCKPIFKKLKGWQCSTADCRKLSDLPENAMNYLRFLAELMEVPIAIVSLGANRDQT
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCE
IVIEDPIHGPKRALLR
EEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA